{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T03:30:25Z","timestamp":1792294225897,"version":"4.4.0"},"reference-count":38,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2021,2,2]],"date-time":"2021-02-02T00:00:00Z","timestamp":1612224000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The Moroccan flora is rich in medicinal plants that are commonly used in folk medicine for the treatment of various diseases. The present study was designed to investigate the total phenolic and flavonoid contents, as well as the antioxidant properties of leaves extracts from Globularia alypum L. colected from the Taza region in northeast Morocco. Additionally, the individual phenolics and volatiles of the extracts were also evaluated. The organic extracts of this plant were obtained by Soxhlet extraction using two different solvents, namely ethyl acetate and chloroform. The antioxidant capacity of leaves extracts was measured using DPPH, ABTS and FRAP assays; the phenolic profile was determined by HPLC-DAD\/ESI-MS analysis, whereas the volatile composition was elucidated by GC-MS. The ethyl acetate extract analysis showed a total of 20 phenolic compounds and the determination of phenolic contents showed a significant value of 56.5 \u00b1 0.61 \u00b5g GAE\/mg of extract in comparison with the chloroform extract (18.9 \u00b1 0.48 \u00b5g GAE\/mg of extract). Also, the determination of the flavonoid contents revealed that the ethyl acetate extract contained the highest value (30.2 \u00b1 0.55 \u00b5g CE\/mg of extract) in comparison with the chloroform extract (18.0 \u00b1 0.36 \u00b5g CE\/mg of extract). Concerning the antioxidant properties, interesting values were attained for the ethyl acetate extract which exhibited higher antioxidant activity, namely IC50 = 12.3 \u00b1 3.83 \u00b5g\/mL and IC50 = 37.0 \u00b1 2.45 \u00b5g\/mL for the DPPH and ABTS assays, respectively, whereas a value of 531.1 \u00b1 17.08 (mg AAE\/g DW) was obtained for the FRAP assay. Concerning the volatile profile, a total of 73 compounds were positively detected and among them n-hexadecanoic acid (13.5%) was the most abundant one. The results achieved confirm the important role of this plant as a source of natural antioxidants.<\/jats:p>","DOI":"10.3390\/molecules26030759","type":"journal-article","created":{"date-parts":[[2021,2,2]],"date-time":"2021-02-02T05:44:42Z","timestamp":1612244682000},"page":"759","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":52,"title":["Phytochemical Investigation and Antioxidant Activity of Globularia alypum L."],"prefix":"10.3390","volume":"26","author":[{"given":"Fadoua","family":"Asraoui","sequence":"first","affiliation":[{"name":"Laboratory of Applied Biology and Pathology, Department of Biology, Faculty of Sciences of Tetouan, Abd Al-Malek Essaadi University, Tetouan 93000, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayoub","family":"Kounnoun","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Biology and Pathology, Department of Biology, Faculty of Sciences of Tetouan, Abd Al-Malek Essaadi University, Tetouan 93000, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0370-5482","authenticated-orcid":false,"given":"Hafssa El","family":"Cadi","sequence":"additional","affiliation":[{"name":"Laboratory of Valorization of Resources and Chemical Engineering, Department of Chemistry, Abdelmalek Essaadi University, Tangier 90000, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1296-7633","authenticated-orcid":false,"given":"Francesco","family":"Cacciola","sequence":"additional","affiliation":[{"name":"Department of Biomedical, Dental, Morphological and Functional Imaging Sciences, University of Messina, 98125 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yassine Oulad El","family":"Majdoub","sequence":"additional","affiliation":[{"name":"Department of Chemical Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filippo","family":"Alibrando","sequence":"additional","affiliation":[{"name":"Chromaleont s.r.l., c\/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filippo","family":"Mandolfino","sequence":"additional","affiliation":[{"name":"Department of Chemical Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168 Messina, Italy"},{"name":"Foundation A. Imbesi c\/o, University of Messina, 98122 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Dugo","sequence":"additional","affiliation":[{"name":"Department of Chemical Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168 Messina, Italy"},{"name":"Chromaleont s.r.l., c\/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8890-675X","authenticated-orcid":false,"given":"Luigi","family":"Mondello","sequence":"additional","affiliation":[{"name":"Department of Chemical Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168 Messina, Italy"},{"name":"Chromaleont s.r.l., c\/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168 Messina, Italy"},{"name":"Department of Sciences and Technologies for Human and Environment, University Campus Bio-Medico of Rome, 00128 Rome, Italy"},{"name":"BeSep s.r.l., c\/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adnane","family":"Louajri","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Biology and Pathology, Department of Biology, Faculty of Sciences of Tetouan, Abd Al-Malek Essaadi University, Tetouan 93000, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,2,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2157","DOI":"10.1016\/j.lfs.2003.09.047","article-title":"Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer","volume":"74","author":"Cai","year":"2004","journal-title":"Life Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.foodchem.2003.05.007","article-title":"Screening of radical scavenging activity of some medicinal and aromatic plant extracts","volume":"85","author":"Miliauskas","year":"2004","journal-title":"Food Chem."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Altemimi, A., Lakhssassi, N., Baharlouei, A., Watson, D.G., and Lightfoot, D.A. (2017). Phytochemicals: Extraction, isolation, and identification of bioactive compounds from plant extracts. Plants, 6.","DOI":"10.3390\/plants6040042"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1203\/PDR.0b013e3181a9eafb","article-title":"Oxygen toxicity and reactive oxygen species: The devil is in the details","volume":"66","author":"Auten","year":"2009","journal-title":"Pediatr. Res."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Satoh, T. (2016). Astaxanthin: Health Benefits and Toxicity in Nutraceuticals, Elsevier.","DOI":"10.1016\/B978-0-12-802147-7.00038-3"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"S1217","DOI":"10.1016\/S2221-1691(12)60388-8","article-title":"Phytochemical screening and antioxidant activity of Lebanese Eryngium creticum L","volume":"2","author":"Farhan","year":"2012","journal-title":"Asian Pac. J. Trop. Biomed."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.ejphar.2008.06.053","article-title":"The impact of high anxiety level on the oxidative status of mouse peripheral blood lymphocytes, granulocytes and monocytes","volume":"589","author":"Rammal","year":"2008","journal-title":"Eur. J. Pharmacol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1859","DOI":"10.1021\/jf0113575","article-title":"Profiling and characterization antioxidant activities in Anoectochilus formosanus Hayata","volume":"50","author":"Wang","year":"2002","journal-title":"J. Agric. Food Chem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1016\/S2221-1691(13)60110-0","article-title":"Chemical composition and antioxidant activity of a Lebanese plant Euphorbia macroclada schyzoceras","volume":"3","author":"Farhan","year":"2013","journal-title":"Asian Pac. J. Trop. Biomed."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Andersen, O.M., and Markham, K.R. (2005). Flavonoids: Chemistry, Biochemistry and Applications, CRC Press.","DOI":"10.1201\/9781420039443"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Hammiche, V., Merad, R., and Azzouz, M. (2013). Plantes Toxiques \u00e0 Usage M\u00e9dicinal du Pourtour M\u00e9diterran\u00e9en, Springer.","DOI":"10.1007\/978-2-8178-0375-3"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/S0378-8741(01)00289-6","article-title":"Ethnobotanical survey of medicinal plants used for the treatment of diabetes, cardiac and renal diseases in the North centre region of Morocco (Fez\u2013Boulemane)","volume":"77","author":"Jouad","year":"2001","journal-title":"J. Ethnopharmacol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1016\/j.jep.2013.03.082","article-title":"Herbalists and wild medicinal plants in M\u2019Sila (North Algeria): An ethnopharmacology survey","volume":"148","author":"Boudjelal","year":"2013","journal-title":"J. Ethnopharmacol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.jaridenv.2015.11.005","article-title":"An ethnopharmacological survey of plants used in traditional diabetes treatment in south-eastern Algeria (Ouargla province)","volume":"127","author":"Telli","year":"2016","journal-title":"J. Arid Environ."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"159","DOI":"10.14419\/ijpt.v4i2.6477","article-title":"Phytochemical composition and antioxidant activity of extracts of some medicinal plants in Tunisia","volume":"4","author":"Athmouni","year":"2016","journal-title":"Int. J. Pharmacol. Toxicol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1111\/aje.12041","article-title":"Polyphenols, flavonoids, antioxidant activity in leaves and flowers of Tunisian Globularia alypum L. (Globulariaceae)","volume":"51","author":"Chograni","year":"2013","journal-title":"Afr. J. Ecol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"274","DOI":"10.4172\/pharmaceutical-sciences.1000355","article-title":"Nutritional Composition, Analysis of Secondary Metabolites and Antioxidative Effects of the Leaves of Globularia alypum L.","volume":"80","author":"Khantouche","year":"2018","journal-title":"Indian J. Pharm. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1016\/j.foodchem.2005.04.028","article-title":"Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds","volume":"97","author":"Djeridane","year":"2006","journal-title":"Food Chem."},{"key":"ref_19","first-page":"478","article-title":"Antioxidant and antimicrobial activities of selected medicinal plants from Algeria","volume":"2","author":"Krimat","year":"2014","journal-title":"J. Coast. Life Med."},{"key":"ref_20","unstructured":"Tiss, M., Souiy, Z., Achour, L., and Hamden, K. (2020). Anti-obesity, anti-hyperglycaemic, anti-antipyretic and analgesic activities of Globularia alypum extracts. Arch. Physiol. Biochem., 1\u20138."},{"key":"ref_21","first-page":"1988","article-title":"In vitro evaluation of the antioxidant and antimicrobial effects of Globularia alypum L. extracts","volume":"7","author":"Taghzouti","year":"2016","journal-title":"J. Mater. Environ. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.foodchem.2005.03.032","article-title":"Antioxidant and free radical scavenging activity of an aqueous extract of Coleus aromaticus","volume":"97","author":"Kumaran","year":"2006","journal-title":"Food Chem."},{"key":"ref_23","first-page":"2","article-title":"Global chemical composition and antioxidative effect of the ethanol extracts prepared from Globularia alypum leaves","volume":"14","author":"Touaibia","year":"2016","journal-title":"Nat. Technol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.jff.2017.02.015","article-title":"Protective effect of Globularia alypum leaves against deltamethrin-induced nephrotoxicity in rats and determination of its bioactive compounds using high-performance liquid chromatography coupled with electrospray ionization tandem quadrupole\u2013time-of-flight mass spectrometry","volume":"32","author":"Feriani","year":"2017","journal-title":"J. Funct. Foods"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1293","DOI":"10.1021\/np0501233","article-title":"Antioxidant constituents of the aerial parts of Globularia a Lypum growing in Morocco","volume":"68","author":"Khlifi","year":"2005","journal-title":"J. Nat. Prod."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1080\/00387010701301576","article-title":"Fragmentation study of globularin through positive and negative ESI\/MS, CID\/MS, and tandem MS\/MS","volume":"40","author":"Kerhoas","year":"2007","journal-title":"Spectrosc. Lett."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1248\/cpb.54.85","article-title":"Iridoid glucosides from the aerial parts of Globularia alypum L. (Globulariaceae)","volume":"54","author":"Khlifi","year":"2006","journal-title":"Chem. Pharm. Bull."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"6299","DOI":"10.1016\/j.arabjc.2020.05.022","article-title":"Wild strawberry (Arbutus unedo): Phytochemical screening and antioxidant properties of fruits collected in northern Morocco","volume":"13","author":"Kounnoun","year":"2020","journal-title":"Arab. J. Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1002\/pca.2506","article-title":"Tentative characterisation of iridoids, phenylethanoid glycosides and flavonoid derivatives from Globularia alypum L. (Globulariaceae) leaves by LC-ESI-QTOF-MS","volume":"25","author":"Madani","year":"2014","journal-title":"Phytochem. Anal."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1016\/S0021-9258(18)84277-6","article-title":"On tyrosine and tryptophane determinations in proteins","volume":"73","author":"Folin","year":"1927","journal-title":"J. Biol. Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"144","DOI":"10.5344\/ajev.1965.16.3.144","article-title":"Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents","volume":"16","author":"Singleton","year":"1965","journal-title":"Am. J. Enol. Vitic."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"3010","DOI":"10.1021\/jf0115589","article-title":"Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity","volume":"50","author":"Dewanto","year":"2002","journal-title":"J. Agric. Food Chem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2090","DOI":"10.1248\/cpb.36.2090","article-title":"Two new flavonoids and other constituents in licorice root: Their relative astringency and radical scavenging effects","volume":"36","author":"Hatano","year":"1988","journal-title":"Chem. Pharm. Bull."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1016\/S0891-5849(98)00315-3","article-title":"Antioxidant activity applying an improved ABTS radical cation decolorization assay","volume":"26","author":"Re","year":"1999","journal-title":"Free Radic. Biol. Med."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"307","DOI":"10.5264\/eiyogakuzashi.44.307","article-title":"Studies on product of browning reaction prepared from glucose amine","volume":"44","year":"1986","journal-title":"Jpn. J. Nutr."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Oulad El Majdoub, Y., Alibrando, F., Cacciola, F., Arena, K., Pagnotta, E., Matteo, R., Micalizzi, G., Dugo, L., Dugo, P., and Mondello, L. (2020). Chemical Characterization of Three Accessions of Brassica juncea L. Extracts from Different Plant Tissues. Molecules, 25.","DOI":"10.3390\/molecules25225421"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1781","DOI":"10.1002\/jssc.202000169","article-title":"Evaluation of matrix effect in one-dimensional and comprehensive two-dimensional liquid chromatography for the determination of the phenolic fraction in extra virgin olive oils","volume":"43","author":"Arena","year":"2020","journal-title":"J. Sep. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"4819","DOI":"10.1007\/s00216-019-01649-w","article-title":"Determination of the polyphenolic fraction of Pistacia vera L. kernel extracts by comprehensive two-dimensional liquid chromatography coupled to mass spectrometry detection","volume":"411","author":"Arena","year":"2019","journal-title":"Anal. Bioanal. Chem."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/26\/3\/759\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:18:42Z","timestamp":1760159922000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/26\/3\/759"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,2,2]]},"references-count":38,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2021,2]]}},"alternative-id":["molecules26030759"],"URL":"https:\/\/doi.org\/10.3390\/molecules26030759","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,2,2]]}}}