{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T15:28:57Z","timestamp":1787758137760,"version":"build-2784847793"},"reference-count":26,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2021,2,2]],"date-time":"2021-02-02T00:00:00Z","timestamp":1612224000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["PP00P2_163683 and PP00P2_190093"],"award-info":[{"award-number":["PP00P2_163683 and PP00P2_190093"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010661","name":"Horizon 2020 Framework Programme","doi-asserted-by":"publisher","award":["rant Agreement No: 676904, ERC-StG-2015, NanoSCAN; 101001734, ERC-CoG-2020, PhotoPHARMA"],"award-info":[{"award-number":["rant Agreement No: 676904, ERC-StG-2015, NanoSCAN; 101001734, ERC-CoG-2020, PhotoPHARMA"]}],"id":[{"id":"10.13039\/100010661","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004361","name":"Krebsliga Schweiz","doi-asserted-by":"publisher","award":["KLS-4257-08-2017"],"award-info":[{"award-number":["KLS-4257-08-2017"]}],"id":[{"id":"10.13039\/501100004361","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>89Zr-radiolabelled proteins functionalised with desferrioxamine B are a cornerstone of diagnostic positron emission tomography. In the clinical setting, 89Zr-labelled proteins are produced manually. Here, we explore the potential of using a microfluidic photochemical flow reactor to prepare 89Zr-radiolabelled proteins. The light-induced functionalisation and 89Zr-radiolabelling of human serum albumin ([89Zr]ZrDFO-PEG3-Et-azepin-HSA) was achieved by flow photochemistry with a decay-corrected radiochemical yield (RCY) of 31.2 \u00b1 1.3% (n = 3) and radiochemical purity &gt;90%. In comparison, a manual batch photoreactor synthesis produced the same radiotracer in a decay-corrected RCY of 59.6 \u00b1 3.6% (n = 3) with an equivalent RCP &gt; 90%. The results indicate that photoradiolabelling in flow is a feasible platform for the automated production of protein-based 89Zr-radiotracers, but further refinement of the apparatus and optimisation of the method are required before the flow process is competitive with manual reactions.<\/jats:p>","DOI":"10.3390\/molecules26030764","type":"journal-article","created":{"date-parts":[[2021,2,2]],"date-time":"2021-02-02T13:01:12Z","timestamp":1612270872000},"page":"764","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Microfluidic Preparation of 89Zr-Radiolabelled Proteins by Flow Photochemistry"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2879-1583","authenticated-orcid":false,"given":"Daniel F.","family":"Earley","sequence":"first","affiliation":[{"name":"Department of Chemistry, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2455-9293","authenticated-orcid":false,"given":"Amaury","family":"Guillou","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dion","family":"van der Born","sequence":"additional","affiliation":[{"name":"FutureChemistry Agro Business Park 10, 6708 PW Wageningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex J.","family":"Poot","sequence":"additional","affiliation":[{"name":"Department of Radiology and Nuclear Medicine, UMC Utrecht, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0066-219X","authenticated-orcid":false,"given":"Jason P.","family":"Holland","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,2,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"197","DOI":"10.3390\/antib4030197","article-title":"Antibody Conjugates: From Heterogeneous Populations to Defined Reagents","volume":"4","author":"Dennler","year":"2015","journal-title":"Antibodies"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2211","DOI":"10.1021\/acs.bioconjchem.7b00325","article-title":"89Zr-Immuno-Positron Emission Tomography in Oncology: State-of-the-Art 89Zr Radiochemistry","volume":"28","author":"Heskamp","year":"2017","journal-title":"Bioconjug. 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