{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T19:54:10Z","timestamp":1791921250991,"version":"4.3.4"},"reference-count":32,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2021,3,10]],"date-time":"2021-03-10T00:00:00Z","timestamp":1615334400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81573573"],"award-info":[{"award-number":["81573573"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81473338"],"award-info":[{"award-number":["81473338"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the Classical Prescription Basic Research Team of the Beijing University of Chinese Medicine","award":["2019-JYB-TD-001"],"award-info":[{"award-number":["2019-JYB-TD-001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Glycyrrhizae Radix et Rhizoma (GRR) is one of the commonly used traditional Chinese medicines in clinical practice, which has been applied to treat digestive system diseases for hundreds of years. GRR is preferred for anti-gastric ulcer, however, the main active compounds are still unknown. In this study, GRR was used as precursor to synthesize carbon dots (CDs) by a environment-friendly one-step pyrolysis process. GRR-CDs were characterized by using transmission electron microscopy, high-resolution TEM, fourier transform infrared, ultraviolet-visible and fluorescence spectroscopy, X-ray photoelectron spectroscopy, X-ray diffraction and high-performance liquid chromatography. In addition, cellular toxicity of GRR-CDs was studied by using CCK-8 in RAW264.7 cells, and the anti-gastric ulcer activity was evaluated and confirmed using mice model of acute alcoholic gastric ulcer. The experiment confirmed that GRR-CDs were the spherical structure with a large number of active groups on the surface and their particle size ranged from 2 to 10 nm. GRR-CDs had no toxicity to RAW264.7 cells at concentration of 19.5 to 5000 \u03bcg\/mL and could reduce the oxidative damage of gastric mucosa and tissues caused by alcohol, as demonstrated by restoring expression of malondialdehyde, superoxide dismutase and nitric oxide in serum and tissue of mice. The results indicated the explicit anti-ulcer activity of GRR-CDs, which provided a new insights for the research on effective material basis of GRR.<\/jats:p>","DOI":"10.3390\/molecules26061512","type":"journal-article","created":{"date-parts":[[2021,3,10]],"date-time":"2021-03-10T20:51:42Z","timestamp":1615409502000},"page":"1512","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["Novel Carbon Dots Derived from Glycyrrhizae Radix et Rhizoma and Their Anti-Gastric Ulcer Effect"],"prefix":"10.3390","volume":"26","author":[{"given":"Yuhan","family":"Liu","sequence":"first","affiliation":[{"name":"School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meiling","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinjun","family":"Cheng","sequence":"additional","affiliation":[{"name":"School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Beijing University of Chinese Medicine, Beijing 100029, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Kong","sequence":"additional","affiliation":[{"name":"School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8323-5264","authenticated-orcid":false,"given":"Yan","family":"Zhao","sequence":"additional","affiliation":[{"name":"School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huihua","family":"Qu","sequence":"additional","affiliation":[{"name":"Center of Scientific Experiment, Beijing University of Chinese Medicine, Beijing 100029, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,10]]},"reference":[{"key":"ref_1","first-page":"1261","article-title":"Clinical experience and academic thoughts of Professor LIU Feng-bin on case series of gastroesophageal reflux disease based on data mining","volume":"43","author":"Hou","year":"2018","journal-title":"Zhongguo Zhong Yao Za Zhi"},{"key":"ref_2","first-page":"269","article-title":"Anti-ulcer and antioxidant activity of GutGard","volume":"48","author":"Mukherjee","year":"2010","journal-title":"Indian J. Exp. Biol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"E74","DOI":"10.1208\/pt060113","article-title":"Licorice: A possible anti-inflammatory and anti-ulcer drug","volume":"6","author":"Aly","year":"2005","journal-title":"AAPS PharmSciTech"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1997","DOI":"10.2174\/0929867325666181001104550","article-title":"A Review: The Anti-inflammatory, Anticancer and Antibacterial Properties of Four Kinds of Licorice Flavonoids Isolated from Licorice","volume":"27","author":"Wang","year":"2020","journal-title":"Curr. Med. Chem."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1177\/146642400012000203","article-title":"Liquorice and its health implications","volume":"120","author":"Olukoga","year":"2000","journal-title":"J. R. Soc. Promot. Health"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1093\/aje\/kwh005","article-title":"Risk of uncomplicated peptic ulcer among users of aspirin and nonaspirin nonsteroidal antiinflammatory drugs","volume":"159","year":"2004","journal-title":"Am. J. Epidemiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1111\/j.2042-7158.1980.tb12879.x","article-title":"Aspirin-induced gastric mucosal damage in rats: Cimetidine and deglycyrrhizinated liquorice together give greater protection than low doses of either drug alone","volume":"32","author":"Bennett","year":"1980","journal-title":"J. Pharm. Pharmacol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/S0021-5198(19)52712-3","article-title":"Effects of FM100, a fraction of licorice root, on serum gastrin concentration in rats and dogs","volume":"32","author":"Ishii","year":"1982","journal-title":"Jpn. J. Pharmacol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"6707","DOI":"10.1021\/acs.jafc.5b02319","article-title":"Green Synthesis of Fluorescent Carbon Dots for Selective Detection of Tartrazine in Food Samples","volume":"63","author":"Xu","year":"2015","journal-title":"J. Agric. Food Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"19153","DOI":"10.1021\/acsami.5b04866","article-title":"Blood Compatibility Evaluations of Fluorescent Carbon Dots","volume":"7","author":"Li","year":"2015","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"10201","DOI":"10.1021\/acsami.5b00405","article-title":"Mitochondria-targeting nanoplatform with fluorescent carbon dots for long time imaging and magnetic field-enhanced cellular uptake","volume":"7","author":"Zhang","year":"2015","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/j.talanta.2016.11.030","article-title":"One pot synthesis of gold\u2014Carbon dots nanocomposite and its application for cytosensing of metals for cancer cells","volume":"166","author":"Abdelhamid","year":"2017","journal-title":"Talanta"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.bios.2017.02.034","article-title":"Fabrication of nitrogen-doped carbon dots for screening the purine metabolic disorder in human fluids","volume":"94","author":"Gowthaman","year":"2017","journal-title":"Biosens. Bioelectron."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.jcis.2015.11.059","article-title":"Carbon dots on based folic acid coated with PAMAM dendrimer as platform for Pt(IV) detection","volume":"465","author":"Campos","year":"2016","journal-title":"J. Colloid Interface Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"12215","DOI":"10.1002\/anie.201206791","article-title":"A biocompatible fluorescent ink based on water-soluble luminescent carbon nanodots","volume":"51","author":"Qu","year":"2012","journal-title":"Angew. Chem. (Int. Ed. Engl.)"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"8510","DOI":"10.1021\/acs.analchem.5b02019","article-title":"Development of a carbon dot (C-Dot)-linked immunosorbent assay for the detection of human \u03b1-fetoprotein","volume":"87","author":"Wu","year":"2015","journal-title":"Anal. Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2925","DOI":"10.2217\/nnm-2019-0255","article-title":"Antihyperuricemic and anti-gouty arthritis activities of Aurantii fructus immaturus carbonisata-derived carbon dots","volume":"14","author":"Wang","year":"2019","journal-title":"Nanomedicine"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1186\/s11671-019-3198-1","article-title":"Protective Effects of Carbon Dots Derived from Phellodendri Chinensis Cortex Carbonisata against Deinagkistrodon acutus Venom-Induced Acute Kidney Injury","volume":"14","author":"Zhang","year":"2019","journal-title":"Nanoscale Res. Lett."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"35586","DOI":"10.1038\/srep35586","article-title":"The ethanopharmacological aspect of carbon nanodots in turmeric smoke","volume":"6","author":"Chun","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1186\/s12951-017-0296-z","article-title":"Hemostatic bioactivity of novel Pollen Typhae Carbonisata-derived carbon quantum dots","volume":"15","author":"Yan","year":"2017","journal-title":"J. Nanobiotechnol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"391","DOI":"10.2217\/nnm-2017-0297","article-title":"Novel Phellodendri Cortex (Huang Bo)-derived carbon dots and their hemostatic effect","volume":"13","author":"Liu","year":"2018","journal-title":"Nanomedicine"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"431","DOI":"10.2217\/nnm-2018-0285","article-title":"Hemostatic and hepatoprotective bioactivity of Junci Medulla Carbonisata-derived Carbon Dots","volume":"14","author":"Cheng","year":"2019","journal-title":"Nanomedicine"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"4139","DOI":"10.2147\/IJN.S248467","article-title":"Effect of Lonicerae japonicae Flos Carbonisata-Derived Carbon Dots on Rat Models of Fever and Hypothermia Induced by Lipopolysaccharide","volume":"15","author":"Wu","year":"2020","journal-title":"Int. J. Nanomed."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1166\/jbn.2019.2675","article-title":"Novel Carbon Dots-Derived from Radix Puerariae Carbonisata Significantly Improve the Solubility and Bioavailability of Baicalin","volume":"15","author":"Luo","year":"2019","journal-title":"J. Biomed. Nanotechnol."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Zhao, Y., Zhang, Y., Kong, H., Zhang, M., Cheng, J., Luo, J., Zhao, Y., and Qu, H. (2020). Haemostatic Nanoparticles-Derived Bioactivity of from Selaginella tamariscina Carbonisata. Molecules, 25.","DOI":"10.3390\/molecules25030446"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"110739","DOI":"10.1016\/j.fct.2019.110739","article-title":"Protective effects of Aronia melanocarpa juices either alone or combined with extracts from Rosa canina or Alchemilla vulgaris in a rat model of indomethacin-induced gastric ulcers","volume":"132","author":"Denev","year":"2019","journal-title":"Food Chem. Toxicol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.cbi.2016.03.003","article-title":"Evaluation of protective effects of costunolide and dehydrocostuslactone on ethanol-induced gastric ulcer in mice based on multi-pathway regulation","volume":"250","author":"Zheng","year":"2016","journal-title":"Chem.-Biol. Interact."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"110539","DOI":"10.1016\/j.fct.2019.05.047","article-title":"Chemical characterization and gastroprotective effect of an isolated polysaccharide fraction from Bletilla striata against ethanol-induced acute gastric ulcer","volume":"131","author":"Zhang","year":"2019","journal-title":"Food Chem. Toxicol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.cbi.2019.05.011","article-title":"Hesperidin, a citrus flavanone glycoside, accelerates the gastric healing process of acetic acid-induced ulcer in rats","volume":"308","author":"Pezzini","year":"2019","journal-title":"Chem.-Biol. Interact."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Alvarez-Suarez, J.M., Dekanski, D., Risti\u0107, S., Radonji\u0107, N.V., Petronijevi\u0107, N.D., Giampieri, F., Astolfi, P., Gonz\u00e1lez-Param\u00e1s, A.M., Santos-Buelga, C., and Tulipani, S. (2011). Strawberry polyphenols attenuate ethanol-induced gastric lesions in rats by activation of antioxidant enzymes and attenuation of MDA increase. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0025878"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1364","DOI":"10.1016\/j.ultrasmedbio.2017.02.008","article-title":"Evaluation of Transabdominal Ultrasound with Oral Cellulose-Based Contrast Agent in the Detection and Surveillance of Gastric Ulcer","volume":"43","author":"Liu","year":"2017","journal-title":"Ultrasound Med. Biol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"3643824","DOI":"10.1155\/2016\/3643824","article-title":"Antioxidant, Anti-inflammatory, and Antiulcer Potential of Manuka Honey against Gastric Ulcer in Rats","volume":"2016","author":"Almasaudi","year":"2016","journal-title":"Oxid. Med. Cell. Longev."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/26\/6\/1512\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:33:28Z","timestamp":1760160808000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/26\/6\/1512"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3,10]]},"references-count":32,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2021,3]]}},"alternative-id":["molecules26061512"],"URL":"https:\/\/doi.org\/10.3390\/molecules26061512","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,3,10]]}}}