{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T01:52:59Z","timestamp":1760233979702,"version":"build-2065373602"},"reference-count":50,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2021,3,12]],"date-time":"2021-03-12T00:00:00Z","timestamp":1615507200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>In this study, we examined the Aureobasidium pullulans strains DSM 14940 and DSM 14941 included in the Blossom Protect\u2122 agent to be used in the bioreduction reaction of a symmetrical dicarbonyl compound. Both chiral 2-hydroxy-1,2-diphenylethanone antipodes were obtained with a high enantiomeric purity. Mild conditions (phosphate buffer [pH 7.0, 7.2], 30 \u00b0C) were successfully employed in the synthesis of (S)-benzoin using two different methodologies: benzyl desymmetrization and rac-benzoin deracemization. Bioreduction carried out with higher reagent concentrations, lower pH values and prolonged reaction time, and in the presence of additives, enabled enrichment of the reaction mixture with (R)-benzoin. The described procedure is a potentially useful tool in the synthesis of chiral building blocks with a defined configuration in a simple and economical process with a lower environmental impact, enabling one-pot biotransformation.<\/jats:p>","DOI":"10.3390\/molecules26061578","type":"journal-article","created":{"date-parts":[[2021,3,14]],"date-time":"2021-03-14T23:52:06Z","timestamp":1615765926000},"page":"1578","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Microbial Synthesis of (S)- and (R)-Benzoin in Enantioselective Desymmetrization and Deracemization Catalyzed by Aureobasidium pullulans Included in the Blossom Protect\u2122 Agent"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7277-721X","authenticated-orcid":false,"given":"Renata","family":"Ko\u0142odziejska","sequence":"first","affiliation":[{"name":"Department of Medical Biology and Biochemistry, Faculty of Medicine, Nicolaus Copernicus University in Toru\u0144, Collegium Medicum in Bydgoszcz, 24 Kar\u0142owicz Street, 85-092 Bydgoszcz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5853-4214","authenticated-orcid":false,"given":"Renata","family":"Studzi\u0144ska","sequence":"additional","affiliation":[{"name":"Department of Organic Chemistry, Faculty of Pharmacy, Nicolaus Copernicus University in Toru\u0144, Collegium Medicum in Bydgoszcz, 2 Jurasz Street, 85-089 Bydgoszcz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2015-7510","authenticated-orcid":false,"given":"Agnieszka","family":"Tafelska-Kaczmarek","sequence":"additional","affiliation":[{"name":"Department of Organic Chemistry, Faculty of Chemistry, Nicolaus Copernicus University in Toru\u0144, 7 Gagarin Street, 87-100 Toru\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanna","family":"Pawluk","sequence":"additional","affiliation":[{"name":"Department of Medical Biology and Biochemistry, Faculty of Medicine, Nicolaus Copernicus University in Toru\u0144, Collegium Medicum in Bydgoszcz, 24 Kar\u0142owicz Street, 85-092 Bydgoszcz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dominika","family":"Mlicka","sequence":"additional","affiliation":[{"name":"Department of Medical Biology and Biochemistry, Faculty of Medicine, Nicolaus Copernicus University in Toru\u0144, Collegium Medicum in Bydgoszcz, 24 Kar\u0142owicz Street, 85-092 Bydgoszcz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4492-4796","authenticated-orcid":false,"given":"Alina","family":"Wo\u017aniak","sequence":"additional","affiliation":[{"name":"Department of Medical Biology and Biochemistry, Faculty of Medicine, Nicolaus Copernicus University in Toru\u0144, Collegium Medicum in Bydgoszcz, 24 Kar\u0142owicz Street, 85-092 Bydgoszcz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1016\/S0960-894X(02)01058-2","article-title":"Inhibitors of A\u03b2 production: Solid-phase synthesis and SAR of \u03b1-hydroxycarbonyl derivatives","volume":"13","author":"Wallace","year":"2003","journal-title":"Bioorganic Med. 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