{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T08:05:37Z","timestamp":1784966737571,"version":"3.55.0"},"reference-count":49,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2021,3,28]],"date-time":"2021-03-28T00:00:00Z","timestamp":1616889600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Croton hirtus L\u2019H\u00e9r methanol extract was studied by NMR and two different LC-DAD-MSn using electrospray (ESI) and atmospheric pressure chemical ionization (APCI) sources to obtain a quali-quantitative fingerprint. Forty different phytochemicals were identified, and twenty of them were quantified, whereas the main constituents were dihydro \u03b1 ionol-O-[arabinosil(1-6) glucoside] (133 mg\/g), dihydro \u03b2 ionol-O-[arabinosil(1-6) glucoside] (80 mg\/g), \u03b2-sitosterol (49 mg\/g), and isorhamnetin-3-O-rutinoside (26 mg\/g). C. hirtus was extracted with different solvents\u2014namely, water, methanol, dichloromethane, and ethyl acetate\u2014and the extracts were assayed using different in vitro tests. The methanolic extracts presented the highest 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2\u2032-azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS), and ferric reducing antioxidant power (FRAP) values. All the tested extracts exhibited inhibitory effects on acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), with a higher activity observed for dichloromethane (AChE: 5.03 and BChE: 16.41 mgGALAE\/g), while the methanolic extract showed highest impact against tyrosinase (49.83 mgKAE\/g). Taken together, these findings suggest C. hirtus as a novel source of bioactive phytochemicals with potential for commercial development.<\/jats:p>","DOI":"10.3390\/molecules26071902","type":"journal-article","created":{"date-parts":[[2021,3,28]],"date-time":"2021-03-28T22:09:01Z","timestamp":1616969341000},"page":"1902","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Evaluation of Antioxidant and Enzyme Inhibition Properties of Croton hirtus L\u2019H\u00e9r. Extracts Obtained with Different Solvents"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8264-6953","authenticated-orcid":false,"given":"Stefano","family":"Dall\u2019Acqua","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Via Marzolo 5, 35131 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kouadio Ibrahime","family":"Sinan","sequence":"additional","affiliation":[{"name":"Department of Biology, Science Faculty, Selcuk University, Campus, 42130 Konya, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefania","family":"Sut","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Via Marzolo 5, 35131 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irene","family":"Ferrarese","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Via Marzolo 5, 35131 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ouattara Katinan","family":"Etienne","sequence":"additional","affiliation":[{"name":"Laboratoire de Botanique, UFR Biosciences, Universit\u00e9 F\u00e9lix Houphou\u00ebt-Boigny, 00225 Abidjan, C\u00f4te d\u2019Ivoire"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3962-8666","authenticated-orcid":false,"given":"Mohamad Fawzi","family":"Mahomoodally","sequence":"additional","affiliation":[{"name":"Department of Health Sciences, Faculty of Medicine and Health Sciences, University of Mauritius, 230 R\u00e9duit, Mauritius"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Devina","family":"Lobine","sequence":"additional","affiliation":[{"name":"Department of Health Sciences, Faculty of Medicine and Health Sciences, University of Mauritius, 230 R\u00e9duit, Mauritius"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6548-7823","authenticated-orcid":false,"given":"Gokhan","family":"Zengin","sequence":"additional","affiliation":[{"name":"Department of Biology, Science Faculty, Selcuk University, Campus, 42130 Konya, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1590\/S0103-50532007000100002","article-title":"Traditional uses, chemistry and pharmacology of Croton species (Euphorbiaceae)","volume":"18","author":"Salatino","year":"2007","journal-title":"J. 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