{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,17]],"date-time":"2026-05-17T07:59:51Z","timestamp":1779004791164,"version":"3.51.4"},"reference-count":49,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T00:00:00Z","timestamp":1617235200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Ascorbic acid (AA) has antioxidant properties. However, in the presence of Fe2+\/Fe3+ ions and H2O2, it may behave as a pro-oxidant by accelerating and enhancing the formation of hydroxyl radicals (\u2022OH). Therefore, in this study we evaluated the effect of AA at concentrations of 1 to 200 \u00b5mol\/L on \u2022OH-induced light emission (at a pH of 7.4 and temperature of 37 \u00b0C) from 92.6 \u00b5mol\/L Fe2+\u2014185.2 \u00b5mol\/L EGTA (ethylene glycol-bis (\u03b2-aminoethyl ether)-N,N,N\u2032,N\u2032-tetraacetic acid)\u20142.6 mmol\/L H2O2, and 92.6 \u00b5mol\/L Fe3+\u2014185.2 \u00b5mol\/L EGTA\u20142.6 mmol\/L H2O2 systems. Dehydroascorbic acid (DHAA) at the same range of concentrations served as the reference compound. Light emission was measured with multitube luminometer (AutoLumat Plus LB 953) for 120 s after automatic injection of H2O2. AA at concentrations of 1 to 50 \u00b5mol\/L and of 1 to 75 \u00b5mol\/L completely inhibited light emission from Fe2+-EGTA-H2O2 and Fe3+-EGTA-H2O2, respectively. Concentrations of 100 and 200 \u00b5mol\/L did not affect chemiluminescence of Fe3+-EGTA-H2O2 but tended to increase light emission from Fe2+-EGTA-H2O2. DHAA at concentrations of 1 to 100 \u00b5mol\/L had no effect on chemiluminescence of both systems. These results indicate that AA at physiological concentrations exhibits strong antioxidant activity in the presence of chelated iron and H2O2.<\/jats:p>","DOI":"10.3390\/molecules26071993","type":"journal-article","created":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T10:44:01Z","timestamp":1617273841000},"page":"1993","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Effect of Physiological Concentrations of Vitamin C on the Inhibitation of Hydroxyl Radical Induced Light Emission from Fe2+-EGTA-H2O2 and Fe3+-EGTA-H2O2 Systems In Vitro"],"prefix":"10.3390","volume":"26","author":[{"given":"Michal","family":"Nowak","sequence":"first","affiliation":[{"name":"Radiation Protection, University Hospital No. 2, Medical University of Lodz, Zeromskiego 113, 90-549 Lodz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wieslaw","family":"Tryniszewski","sequence":"additional","affiliation":[{"name":"Department of Radiological and Isotopic Diagnostics and Therapy, Medical University of Lodz, Zeromskiego 113, 90-549 Lodz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agata","family":"Sarniak","sequence":"additional","affiliation":[{"name":"Department of General Physiology, Medical University of Lodz, Mazowiecka 6\/8, 92-215 Lodz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Wlodarczyk","sequence":"additional","affiliation":[{"name":"Department of Sleep Medicine and Metabolic Disorders, Medical University of Lodz, Mazowiecka 6\/8, 92-215 Lodz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8876-0682","authenticated-orcid":false,"given":"Piotr J.","family":"Nowak","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Hypertension, and Kidney Transplantation, Medical University of Lodz, Pomorska 251, 92-213 Lodz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3445-6930","authenticated-orcid":false,"given":"Dariusz","family":"Nowak","sequence":"additional","affiliation":[{"name":"Department of Clinical Physiology, Medical University of Lodz, Mazowiecka 6\/8, 92-215 Lodz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,4,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3460","DOI":"10.2174\/138161211798072463","article-title":"Importance of iron chelation in free radical-induced oxidative stress and human disease","volume":"17","author":"Jomova","year":"2011","journal-title":"Curr. 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