{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T07:43:48Z","timestamp":1782978228572,"version":"3.54.5"},"reference-count":41,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2021,5,22]],"date-time":"2021-05-22T00:00:00Z","timestamp":1621641600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002261","name":"Russian Foundation for Basic Research","doi-asserted-by":"publisher","award":["19-315-90008"],"award-info":[{"award-number":["19-315-90008"]}],"id":[{"id":"10.13039\/501100002261","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002261","name":"Russian Foundation for Basic Research","doi-asserted-by":"publisher","award":["20-03-00234"],"award-info":[{"award-number":["20-03-00234"]}],"id":[{"id":"10.13039\/501100002261","id-type":"DOI","asserted-by":"publisher"}]},{"name":"state order","award":["0259-2019-0004 \u042101"],"award-info":[{"award-number":["0259-2019-0004 \u042101"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>(1) Recently, metabolic profiling of the tissue in the native state or extracts of its metabolites has become increasingly important in the field of metabolomics. An important factor, in this case, is the presence of blood in a tissue sample, which can potentially lead to a change in the concentration of tissue metabolites and, as a result, distortion of experimental data and their interpretation. (2) In this paper, the metabolomic profiling based on NMR spectroscopy was performed to determine the effect of blood contained in the studied samples of brain tissue on their metabolomic profile. We used 13 male laboratory CD-1\u00ae IGS mice for this study. The animals were divided into two groups. The first group of animals (n = 7) was subjected to the perfusion procedure, and the second group of animals (n = 6) was not perfused. The brain tissues of the animals were homogenized, and the metabolite fraction was extracted with a water\/methanol\/chloroform solution. Samples were studied by high-frequency 1H-NMR spectroscopy with subsequent statistical data analysis. The group comparison was performed with the use of the Student\u2019s test. We identified 36 metabolites in the brain tissue with the use of NMR spectroscopy. (3) For the major set of studied metabolites, no significant differences were found in the brain tissue metabolite concentrations in the native state and after the blood removal procedure. (4) Thus, it was shown that the presence of blood does not have a significant effect on the metabolomic profile of the brain in animals without pathologies.<\/jats:p>","DOI":"10.3390\/molecules26113096","type":"journal-article","created":{"date-parts":[[2021,5,24]],"date-time":"2021-05-24T00:01:20Z","timestamp":1621814480000},"page":"3096","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["The Effect of Blood Contained in the Samples on the Metabolomic Profile of Mouse Brain Tissue: A Study by NMR Spectroscopy"],"prefix":"10.3390","volume":"26","author":[{"given":"Anastasia","family":"Glinskikh","sequence":"first","affiliation":[{"name":"The Federal Research Center Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Academician Lavrentiev Avenue, 10, 630090 Novosibirsk, Russia"},{"name":"International Tomography Center, Siberian Branch of the Russian Academy of Sciences, Institutskaya str. 3a, 630090 Novosibirsk, Russia"},{"name":"Faculty of Fundamental Medicine, Novosibirsk State University, Pirogova str. 2, 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"Snytnikova","sequence":"additional","affiliation":[{"name":"International Tomography Center, Siberian Branch of the Russian Academy of Sciences, Institutskaya str. 3a, 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6124-9710","authenticated-orcid":false,"given":"Ekaterina","family":"Zelentsova","sequence":"additional","affiliation":[{"name":"International Tomography Center, Siberian Branch of the Russian Academy of Sciences, Institutskaya str. 3a, 630090 Novosibirsk, Russia"},{"name":"Faculty of Fundamental Medicine, Novosibirsk State University, Pirogova str. 2, 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Borisova","sequence":"additional","affiliation":[{"name":"The Federal Research Center Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Academician Lavrentiev Avenue, 10, 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1380-3000","authenticated-orcid":false,"given":"Yuri","family":"Tsentalovich","sequence":"additional","affiliation":[{"name":"International Tomography Center, Siberian Branch of the Russian Academy of Sciences, Institutskaya str. 3a, 630090 Novosibirsk, Russia"},{"name":"Faculty of Fundamental Medicine, Novosibirsk State University, Pirogova str. 2, 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrey","family":"Akulov","sequence":"additional","affiliation":[{"name":"The Federal Research Center Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Academician Lavrentiev Avenue, 10, 630090 Novosibirsk, Russia"},{"name":"International Tomography Center, Siberian Branch of the Russian Academy of Sciences, Institutskaya str. 3a, 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Lutz, N.W., Sweedler, J.V., and Weyers, R.A. 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