{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T20:20:38Z","timestamp":1792182038211,"version":"4.4.0"},"reference-count":63,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2021,6,15]],"date-time":"2021-06-15T00:00:00Z","timestamp":1623715200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Artemisia ludoviciana subsp. mexicana has been traditionally used for the treatment of digestive ailments such as gastritis, whose main etiological agent is Helicobacter pylori. In a previous screening study, the aqueous extract exhibited a good in vitro anti-H. pylori activity. With the aim of determining the efficacy of this species as a treatment for H. pylori related diseases and finding bioactive compounds, its aqueous extract was subjected to solvent partitioning and the fractions obtained were tested for their in vitro anti-H. pylori effect, as well as for their in vivo gastroprotective and anti-inflammatory activities. The aqueous extract showed a MIC = 250 \u00b5g\/mL. No acute toxicity was induced in mice. A gastroprotection of 69.8 \u00b1 3.8%, as well as anti-inflammatory effects of 47.6 \u00b1 12.4% and 38.8 \u00b1 10.2% (by oral and topical administration, respectively), were attained. Estafiatin and eupatilin were isolated and exhibited anti-H. pylori activity with MBCs of 15.6 and 31.2 \u00b5g\/mL, respectively. The finding that A. ludoviciana aqueous extract has significant anti-H. pylori, gastroprotective and anti-inflammatory activities is a relevant contribution to the ethnopharmacological knowledge of this species. This work is the first report about the in vivo gastroprotective activity of A. ludoviciana and the anti-H. pylori activity of eupatilin and estafiatin.<\/jats:p>","DOI":"10.3390\/molecules26123654","type":"journal-article","created":{"date-parts":[[2021,6,15]],"date-time":"2021-06-15T21:24:29Z","timestamp":1623792269000},"page":"3654","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Anti-Helicobacter pylori Activity of Artemisia ludoviciana subsp. mexicana and Two of Its Bioactive Components, Estafiatin and Eupatilin"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9650-004X","authenticated-orcid":false,"given":"Juan Francisco","family":"Palacios-Espinosa","sequence":"first","affiliation":[{"name":"Departamento de Sistemas Biol\u00f3gicos, Divisi\u00f3n de Ciencias Biol\u00f3gicas y de la Salud, Universidad Aut\u00f3noma Metropolitana-Xochimilco (UAM-X), Ciudad de M\u00e9xico 04960, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo No\u00e9","family":"N\u00fa\u00f1ez-Arag\u00f3n","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico (UNAM), Ciudad Universitaria, Ciudad de M\u00e9xico 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5506-1367","authenticated-orcid":false,"given":"Erika","family":"Gomez-Chang","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico (UNAM), Ciudad Universitaria, Ciudad de M\u00e9xico 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edelmira","family":"Linares","sequence":"additional","affiliation":[{"name":"Jard\u00edn Bot\u00e1nico, Instituto de Biolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico (UNAM), Ciudad Universitaria, Ciudad de M\u00e9xico 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Bye","sequence":"additional","affiliation":[{"name":"Jard\u00edn Bot\u00e1nico, Instituto de Biolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico (UNAM), Ciudad Universitaria, Ciudad de M\u00e9xico 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2317-5466","authenticated-orcid":false,"given":"Irma","family":"Romero","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico (UNAM), Ciudad Universitaria, Ciudad de M\u00e9xico 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"868","DOI":"10.1111\/apt.14561","article-title":"Systematic review with meta-analysis: The worldwide prevalence of Helicobacter pylori infection","volume":"47","author":"Zamani","year":"2018","journal-title":"Aliment. Pharmacol. Ther."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1146\/annurev.pathol.1.110304.100125","article-title":"The Pathogenesis of Helicobacter pylori\u2013Induced Gastro-Duodenal Diseases","volume":"1","author":"Atherton","year":"2006","journal-title":"Annu. Rev. Pathol. Mech. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"3204","DOI":"10.3748\/wjg.v24.i29.3204","article-title":"Helicobacter pyloriand extragastric diseases: A review","volume":"24","author":"Gravina","year":"2018","journal-title":"World J. Gastroenterol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1941","DOI":"10.1002\/ijc.31937","article-title":"Estimating the global cancer incidence and mortality in 2018: GLOBOCAN sources and methods","volume":"144","author":"Ferlay","year":"2019","journal-title":"Int. J. Cancer"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.bj.2015.06.002","article-title":"Helicobacter pylori infection: An overview of bacterial virulence factors and pathogenesis","volume":"39","author":"Kao","year":"2016","journal-title":"Biomed. J."},{"key":"ref_6","first-page":"151","article-title":"The Role of Host Genetic Polymorphisms in Helicobacter pylori Mediated Disease Outcome","volume":"Volume 11","author":"Kamiya","year":"2019","journal-title":"Helicobacter pylori in Human Diseases"},{"key":"ref_7","first-page":"173","article-title":"Helicobacter pylori Genetic Polymorphisms in Gastric Disease Development","volume":"Volume 11","author":"Kamiya","year":"2019","journal-title":"Helicobacter pylori in Human Diseases"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"721","DOI":"10.12688\/f1000research.14149.1","article-title":"Current understanding and management of Helicobacter pylori infection: An updated appraisal","volume":"7","author":"Ansari","year":"2018","journal-title":"F1000Research"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1111\/1541-4337.12355","article-title":"Natural Products for the Prevention and Management ofHelicobacter pyloriInfection","volume":"17","author":"Liu","year":"2018","journal-title":"Compr. Rev. Food Sci. Food Saf."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Salehi, B., Sharopov, F., Martorell, M., Rajkovic, J., Ademiluyi, A.O., Sharifi-Rad, M., Fokou, P.V.T., Martins, N., Iriti, M., and Sharifi-Rad, J. (2018). Phytochemicals in Helicobacter pylori Infections: What Are We Doing Now?. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19082361"},{"key":"ref_11","unstructured":"(2021, April 15). WHO Global Report on Traditional and Complementary Medicine 2019, Available online: https:\/\/www.who.int\/publications\/i\/item\/978924151536."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1016\/j.jep.2008.12.021","article-title":"Anti-Helicobacter pylori activity of plants used in Mexican traditional medicine for gastrointestinal disorders","volume":"122","author":"Linares","year":"2009","journal-title":"J. Ethnopharmacol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1016\/j.jep.2012.07.001","article-title":"Contribution to the ethnopharmacological and anti-Helicobacter pylori knowledge of Cyrtocarpa procera Kunth (Anacardiaceae)","volume":"143","author":"Espinosa","year":"2012","journal-title":"J. Ethnopharmacol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"990","DOI":"10.1016\/j.jep.2013.12.012","article-title":"Evidence of the anti-Helicobacter pylori, gastroprotective and anti-inflammatory activities of Cuphea aequipetala infusion","volume":"151","author":"Linares","year":"2014","journal-title":"J. Ethnopharmacol."},{"key":"ref_15","unstructured":"(2021, March 23). The Plant List. Version 1.1. Available online: http:\/\/www.theplantlist.org\/tpl1.1\/record\/gcc-67684."},{"key":"ref_16","unstructured":"Argueta, A., Cano, L.M., and Rodarte, M.E. (1994). Atlas de las Plantas de la Medicina Tradicional Mexicana, Tomo I-III, Instituto Nacional Indigenista. [1st ed.]."},{"key":"ref_17","first-page":"95","article-title":"Ethnomedicinal plants used for the treatment of dermatological affections on the Pur\u00e9pecha Plateau, Michoac\u00e1n, Mexico","volume":"125","year":"2018","journal-title":"Acta Bot. Mex."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1007\/BF02862021","article-title":"The market medicinal plants of Monterrey, Nuevo Le\u00f3n, M\u00e9xico","volume":"47","author":"Nicholson","year":"1991","journal-title":"Econ. Bot."},{"key":"ref_19","unstructured":"Osuna-Torres, L., Tapia P\u00e9rez, M.E., and Aguilar Contreras, A. (2005). Plantas Medicinales de la Medicina Tradicional Mexicana Para Tratar Afecciones Gastrointestinales: Estudio Etnobot\u00e1nico, Fitoqu\u00edmico y Farmacol\u00f3gico, Publicacions i Edicions de la Universitat de Barcelona."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-3-030-01099-7_1","article-title":"Chemistry and Biology of Selected Mexican Medicinal Plants","volume":"Volume 108","author":"Kinghorn","year":"2019","journal-title":"Progress in the Chemistry of Organic Natural Products"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.jep.2016.12.038","article-title":"Use of medicinal plants by health professionals in Mexico","volume":"198","author":"Dominguez","year":"2017","journal-title":"J. Ethnopharmacol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1285","DOI":"10.1016\/S0040-4020(01)98591-6","article-title":"Estafiatin, a new sesquiterpene lactone isolated from Artemisia mexicana (Willd)","volume":"19","author":"Romo","year":"1963","journal-title":"Tetrahedron"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0031-9422(00)85153-5","article-title":"Sesquiterpene lactones of artemisia constituents of A. ludoviciana SSP. mexicana","volume":"9","author":"Lee","year":"1970","journal-title":"Phytochemistry"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.1016\/0031-9422(93)85032-M","article-title":"Sesquiterpene lactones and flavonoids from Artemisia ludoviciana ssp. mexicana","volume":"33","author":"Cano","year":"1993","journal-title":"Phytochemistry"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1732","DOI":"10.1016\/j.phytochem.2008.02.014","article-title":"Screening of chemical composition, antimicrobial and antioxidant activities of Artemisia essential oils","volume":"69","author":"Alviano","year":"2008","journal-title":"Phytochemistry"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1002\/ptr.1377","article-title":"Activity against multidrug-resistantMycobacterium tuberculosis in Mexican plants used to treat respiratory diseases","volume":"17","author":"Meckes","year":"2003","journal-title":"Phytotherapy Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.jep.2005.02.022","article-title":"Antibacterial properties of some plants used in Mexican traditional medicine for the treatment of gastrointestinal disorders","volume":"100","author":"Calzada","year":"2005","journal-title":"J. Ethnopharmacol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1007\/s11274-005-9087-z","article-title":"Extracts of Acacia farnesiana and Artemisia ludoviciana inhibit growth, enterotoxin production and adhesion of Vibrio cholerae","volume":"22","author":"Garcia","year":"2006","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"M421","DOI":"10.1111\/j.1750-3841.2011.02229.x","article-title":"Extracts of Edible and Medicinal Plants in Inhibition of Growth, Adherence, and Cytotoxin Production of Campylobacter jejuni and Campylobacter coli","volume":"76","author":"Castillo","year":"2011","journal-title":"J. Food Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/j.jep.2006.05.025","article-title":"In vitro susceptibility of Entamoeba histolytica and Giardia lamblia to plants used in Mexican traditional medicine for the treatment of gastrointestinal disorders","volume":"108","author":"Calzada","year":"2006","journal-title":"J. Ethnopharmacol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1016\/j.fitote.2005.04.009","article-title":"In vitro antiprotozoal activity of the leaves of Artemisia ludoviciana","volume":"76","author":"Guerra","year":"2005","journal-title":"Fitoterapia"},{"key":"ref_32","first-page":"3","article-title":"Antimalaric effect of an alcoholic extract of Artemisia ludoviciana mexicana in a rodent malaria model","volume":"39","author":"Delgado","year":"1997","journal-title":"Parassitologia"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1076\/phbi.40.4.263.8465","article-title":"Antidiarrhoeal Activity of Nonanal, an Aldehyde Isolated from Artemisia ludoviciana","volume":"40","year":"2002","journal-title":"Pharm. Biol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1016\/j.jep.2011.11.041","article-title":"Relaxant effects of Artemisia ludoviciana on isolated rat smooth muscle tissues","volume":"139","year":"2012","journal-title":"J. Ethnopharmacol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/j.jep.2016.01.008","article-title":"Antinociceptive activity of the essential oil from Artemisia ludoviciana","volume":"179","author":"Bye","year":"2016","journal-title":"J. Ethnopharmacol."},{"key":"ref_36","first-page":"1531","article-title":"Quantitative Analysis and Pharmacological Effects of Artemisia ludoviciana Aqueous Extract and Compounds","volume":"12","author":"Mata","year":"2017","journal-title":"Nat. Prod. Commun."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1016\/j.jep.2014.05.051","article-title":"Hypoglycemic properties of some preparations and compounds from Artemisia ludoviciana Nutt","volume":"155","author":"Mata","year":"2014","journal-title":"J. Ethnopharmacol."},{"key":"ref_38","first-page":"361","article-title":"Medicinal plants from Argentina with gastro protective activity","volume":"48","author":"Bucciarelli","year":"2007","journal-title":"Ars Pharm."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2013\/796405","article-title":"In Vivo Models Used for Evaluation of Potential Antigastroduodenal Ulcer Agents","volume":"2013","author":"Adinortey","year":"2013","journal-title":"Ulcers"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"10368","DOI":"10.3748\/wjg.v20.i30.10368","article-title":"Medicinal plant activity onHelicobacter pylorirelated diseases","volume":"20","author":"Wang","year":"2014","journal-title":"World J. Gastroenterol."},{"key":"ref_41","first-page":"1","article-title":"Animal Models as Tools to Investigate Antidiabetic and Anti-Inflammatory Plants","volume":"2012","author":"Eddouks","year":"2012","journal-title":"Evidence-Based Complement. Altern. Med."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/S0014-5793(96)01502-5","article-title":"Sesquiterpene lactone containing Mexican Indian medicinal plants and pure sesquiterpene lactones as potent inhibitors of transcription factor NF-\u03baB","volume":"402","author":"Bork","year":"1997","journal-title":"FEBS Lett."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"153","DOI":"10.22270\/jddt.v10i4.4253","article-title":"Gastroprotective effect and in vitro anti-oxidant activities of the aqueous extract from Artemisia absinthium L aerial parts","volume":"10","author":"Kaoudoune","year":"2020","journal-title":"J. Drug Deliv. Ther."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1016\/j.biopha.2018.01.118","article-title":"The aqueous extract from Artemisia capillaris inhibits acute gastric mucosal injury by inhibition of ROS and NF-kB","volume":"99","author":"Yeo","year":"2018","journal-title":"Biomed. Pharmacother."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2395","DOI":"10.1016\/j.ijbiomac.2017.10.127","article-title":"Gastroprotective effects and structural characterization of a pectic fraction isolated from Artemisia campestris subsp maritima","volume":"107","author":"Dallazen","year":"2018","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"549","DOI":"10.3109\/13880209.2015.1056312","article-title":"Antinociceptive and anti-inflammatory effects of the aerial parts ofArtemisia dracunculusin mice","volume":"54","author":"Eidi","year":"2015","journal-title":"Pharm. Biol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1561","DOI":"10.1007\/s12272-011-0919-0","article-title":"Inhibition of 5-lipoxygenase and skin inflammation by the aerial parts of Artemisia capillaris and its constituents","volume":"34","author":"Kwon","year":"2011","journal-title":"Arch. Pharmacal Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"976","DOI":"10.1111\/j.1440-1746.2008.05333.x","article-title":"Artemisia asiatica extracts protect against ethanol-induced injury in gastric mucosa of rats","volume":"23","author":"Park","year":"2008","journal-title":"J. Gastroenterol. Hepatol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"4293","DOI":"10.1002\/jps.23270","article-title":"Interindividual variation in transdermal and oral drug deliveries","volume":"101","author":"Levin","year":"2012","journal-title":"J. Pharm. Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/0031-9422(82)80045-9","article-title":"Flavonoids from Artemisia ludoviciana var. ludoviciana","volume":"21","author":"Liu","year":"1982","journal-title":"Phytochemistry"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/10286020.2018.1492565","article-title":"Eupatilin: A natural pharmacologically active flavone compound with its wide range applications","volume":"22","author":"Nageen","year":"2018","journal-title":"J. Asian Nat. Prod. Res."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1016\/j.apjtm.2016.04.016","article-title":"Chemical constituents, cytotoxic, antifungal and antimicrobial properties of Centaurea diluta Ait. subsp. algeriensis (Coss. & Dur.) Maire","volume":"9","author":"Zater","year":"2016","journal-title":"Asian Pac. J. Trop. Med."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1701","DOI":"10.1007\/s10068-016-0261-x","article-title":"Sesquiterpene lactones and scopoletins from Artemisia scoparia Waldst. & Kit. and their angiotensin I-converting enzyme inhibitory activities","volume":"25","author":"Cho","year":"2016","journal-title":"Food Sci. Biotechnol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.phytochem.2017.11.010","article-title":"The natural sesquiterpene lactones arglabin, grosheimin, agracin, parthenolide, and estafiatin inhibit T cell receptor (TCR) activation","volume":"146","author":"Schepetkin","year":"2018","journal-title":"Phytochemistry"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1016\/j.jep.2009.04.051","article-title":"Antimicrobial activity of Artemisia douglasiana and dehydroleucodine against Helicobacter pylori","volume":"124","author":"Vega","year":"2009","journal-title":"J. Ethnopharmacol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"4594","DOI":"10.1128\/AAC.00407-12","article-title":"Anti-Helicobacter pylori Potential of Artemisinin and Its Derivatives","volume":"56","author":"Goswami","year":"2012","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_57","unstructured":"(2021, March 23). Missouri Botanical Garden, Tropicos: St. Louis, MO, USA. Available online: http:\/\/www.tropicos.org."},{"key":"ref_58","first-page":"813","article-title":"Antioxidant and anticholinesterase con-stituents of Salvia poculata","volume":"33","author":"Kolak","year":"2009","journal-title":"Turk. J. Chem."},{"key":"ref_59","unstructured":"CLSI (2015). Methods for Antimicrobial Dilution and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria, Clinical and Laboratory Standards Institute. [3rd ed.]. CLSI guideline M45."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-Dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1021\/ac60252a045","article-title":"Phenol-hypochlorite reaction for determination of ammonia","volume":"39","author":"Weatherburn","year":"1967","journal-title":"Anal. Chem."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1007\/BF01234480","article-title":"A new approach to practical acute toxicity testing","volume":"54","author":"Lorke","year":"1983","journal-title":"Arch. Toxicol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.jep.2006.07.037","article-title":"Anti-inflammatory activity of hexane leaf extract of Aspilia africana C.D. Adams","volume":"109","author":"Okoli","year":"2007","journal-title":"J. Ethnopharmacol."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/26\/12\/3654\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:16:28Z","timestamp":1760163388000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/26\/12\/3654"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,15]]},"references-count":63,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2021,6]]}},"alternative-id":["molecules26123654"],"URL":"https:\/\/doi.org\/10.3390\/molecules26123654","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,15]]}}}