{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T19:22:17Z","timestamp":1790882537171,"version":"4.1.0"},"reference-count":51,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2021,7,2]],"date-time":"2021-07-02T00:00:00Z","timestamp":1625184000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The use of natural compounds with biocidal activity to fight the growth of bacteria responsible for foodborne illness is one of the main research challenges in the food sector. This study reports the preparation and physicochemical characterization of chitosan nanoparticles loaded with Thymus capitatus (Th-CNPs) and Origanum vulgare (Or-CNPs) essential oils. The nanosystems were obtained by ionotropic gelation technique with high encapsulation efficiency (80\u201383%) and loading capacity (26\u201327%). Nanoparticles showed a spherical shape, bimodal particle size distribution, and good stability (zeta potential values &gt; 40 mV). The treatment of the nanosuspensions at different temperatures (4 and 40 \u00b0C) and storage times (7, 15, 21, and 30 days) did not affect their physicochemical parameters and highlights their reservoir ability for essential oils also under stressful conditions. Both Or-CNPs and Th-CNPs exhibited an enhanced bactericidal activity against foodborne pathogens (S. aureus, E. coli, L. monocytogenes) than pure essential oils. These ecofriendly nanosystems could represent a valid alternative to synthetic preservatives and be of interest for health and food safety.<\/jats:p>","DOI":"10.3390\/molecules26134055","type":"journal-article","created":{"date-parts":[[2021,7,2]],"date-time":"2021-07-02T10:06:34Z","timestamp":1625220394000},"page":"4055","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":109,"title":["Oregano and Thyme Essential Oils Encapsulated in Chitosan Nanoparticles as Effective Antimicrobial Agents against Foodborne Pathogens"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9428-3985","authenticated-orcid":false,"given":"Giuseppe","family":"Granata","sequence":"first","affiliation":[{"name":"Istituto di Chimica Biomolecolare-C.N.R., Via Paolo Gaifami 18, 95126 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0653-9560","authenticated-orcid":false,"given":"Stefano","family":"Stracquadanio","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Biotechnological Sciences, University of Catania, Via S. Sofia 97, 95123 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9045-4289","authenticated-orcid":false,"given":"Marco","family":"Leonardi","sequence":"additional","affiliation":[{"name":"Istituto di Chimica Biomolecolare-C.N.R., Via Paolo Gaifami 18, 95126 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4281-3256","authenticated-orcid":false,"given":"Edoardo","family":"Napoli","sequence":"additional","affiliation":[{"name":"Istituto di Chimica Biomolecolare-C.N.R., Via Paolo Gaifami 18, 95126 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7483-3070","authenticated-orcid":false,"given":"Graziella","family":"Malandrino","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University off Catania, Via A. Doria 6, 95125 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viviana","family":"Cafiso","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Biotechnological Sciences, University of Catania, Via S. Sofia 97, 95123 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefania","family":"Stefani","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Biotechnological Sciences, University of Catania, Via S. Sofia 97, 95123 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5850-0372","authenticated-orcid":false,"given":"Corrada","family":"Geraci","sequence":"additional","affiliation":[{"name":"Istituto di Chimica Biomolecolare-C.N.R., Via Paolo Gaifami 18, 95126 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,7,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1532","DOI":"10.1080\/10408398.2013.777021","article-title":"Most common foodborne pathogens and mycotoxins on fresh produce: A review of recent outbreaks","volume":"56","author":"Yeni","year":"2016","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/bs.afnr.2018.02.006","article-title":"Listeria monocytogenes in Foods","volume":"86","author":"Jordan","year":"2018","journal-title":"Adv. Food Nutr. Res."},{"key":"ref_3","unstructured":"Reineccius, G. (2016). Flavor Chemistry and Technology, CRC Press\/Taylor & Francis Group. [2nd ed.]."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.ijfoodmicro.2004.03.022","article-title":"Essential oils: Their antibacterial properties and potential applications in foods\u2014A review","volume":"94","author":"Burt","year":"2004","journal-title":"Int. J. Food Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.meatsci.2007.09.005","article-title":"Effect of dietary oregano oil supplementation on lamb meat characteristics","volume":"79","author":"Simitzis","year":"2008","journal-title":"Meat Sci."},{"key":"ref_6","unstructured":"Rai, M., and Kon, K. (2013). Fighting Multidrug Resistance with Herbal Extracts, Essential Oils and Their Components, Academic Press."},{"key":"ref_7","unstructured":"Kon, K., and Rai, M. (2016). A natural alternative to combat antibiotics resistance. Antibiotic Resistance-Mechanisms and New Antimicrobial Approaches, Elsevier Inc."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"e1900677","DOI":"10.1002\/cbdv.201900677","article-title":"New tricks for old guys: Recent developments in the chemistry, biochemistry, applications and exploitation of selected species from the Lamiaceae Family","volume":"17","author":"Napoli","year":"2020","journal-title":"Chem. Biodivers."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.tifs.2020.04.025","article-title":"Chemistry, bioactivities, mode of action and industrial applications of essential oils","volume":"101","author":"Jugreet","year":"2020","journal-title":"Trends Food Sci. Technol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"601","DOI":"10.4315\/0362-028X-63.5.601","article-title":"Antimicrobial effect of herb extracts against Escherichia coli O157: H7, Listeria monocytogenes, and Salmonella typhimurium Associated with Beef","volume":"63","author":"Cutter","year":"2000","journal-title":"J. Food Prot."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"3989","DOI":"10.3390\/molecules17043989","article-title":"Essential oils in combination and their antimicrobial properties","volume":"17","author":"Juliani","year":"2012","journal-title":"Molecules"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.cofs.2015.05.008","article-title":"The antimicrobial paradox: Why preservatives lose activity in foods","volume":"4","author":"Weiss","year":"2015","journal-title":"Curr. Opin. Food Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1023\/A:1008934020409","article-title":"Ecophysiological attributes of Salmonella typhimurium in liquid culture and within a gelatin gel with or without the addition of oregano essential oil","volume":"16","author":"Skandamis","year":"2000","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Weiss, J., Gaysinsky, S., Davidson, M., and McClements, J. (2009). Nanostructured encapsulation systems: Food antimicrobials. Global Issues in Food Science and Technology, Academic Press.","DOI":"10.1016\/B978-0-12-374124-0.00024-7"},{"key":"ref_15","unstructured":"FDA (2021, June 06). GRAS Notices: Shrimp-Derived Chitosan, Available online: http:\/\/www.accessdata.fda.gov\/scripts\/fdcc\/?set=GRASNotices&id=443&sort=GRN_No&order=DESC&startrow=1&type=basic&search=chitosan."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.ijbiomac.2017.12.078","article-title":"A review on chitosan and its nanocomposites in drug delivery","volume":"109","author":"Ali","year":"2018","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Guti\u00e9rrez, T.J. (2017). Chitosan applications for the food industry. Chitosan: Derivatives, Composites and Applications, Scrivener Publishing, LLC\/Wiley.","DOI":"10.1002\/9781119364849.ch8"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1016\/j.lwt.2015.03.049","article-title":"Effect of chitosan nanoparticles loaded with cinnamon essential oil on the quality of chilled pork","volume":"63","author":"Hu","year":"2015","journal-title":"LWT Food Sci. Technol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"5112","DOI":"10.1002\/jsfa.9050","article-title":"Chitosan nanoparticles-loaded Citrus aurantium essential oil: A novel delivery system for preserving the postharvest quality of Agaricus bisporus","volume":"98","author":"Karimirad","year":"2018","journal-title":"J. Sci. Food Agric."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.lwt.2016.11.022","article-title":"Physicochemical characterization of chitosan nanoparticles and nanocapsules incorporated with lime essential oil and their antibacterial activity against food-borne pathogens","volume":"77","year":"2017","journal-title":"LWT Food Sci. Technol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.tifs.2018.05.019","article-title":"Chitosan coatings enriched with essential oils: Effects on fungi involved in fruit decay and mechanisms of action","volume":"78","author":"Serio","year":"2018","journal-title":"Trends Food Sci. Technol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1590\/fst.34519","article-title":"Antibacterial activity of essential oils encapsulated in chitosan nanoparticles","volume":"40","year":"2020","journal-title":"Food Sci. Technol. Campinas"},{"key":"ref_23","first-page":"1781","article-title":"Enhancement of thermal stability and antioxidant activity of thyme essential oil by encapsulation in chitosan nanoparticles","volume":"18","author":"Barzegar","year":"2016","journal-title":"J. Agric. Sci. Technol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"116075","DOI":"10.1016\/j.carbpol.2020.116075","article-title":"Chitosan nanoparticles loaded with clove essential oil: Characterization, antioxidant and antibacterial activities","volume":"236","author":"Hadidi","year":"2020","journal-title":"Carbohydr. Polym."},{"key":"ref_25","unstructured":"(2021, June 06). Electronic Code of Federal Regulations (e-CFR), Available online: https:\/\/www.ecfr.gov\/cgi-bin\/text-idx?SID=42bb02884fc7049a74766606865e2a61&mc=true&node=pt21.6.582&rgn=div5."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/j.carbpol.2007.07.009","article-title":"A review of several reported procedures to determine the degree of N-acetylation for chitin and chitosan using infrared spectroscopy","volume":"71","author":"Kasaai","year":"2008","journal-title":"Carbohydr. Polym."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Foster, L.J.R., Ho, S., Hook, J., Basuki, M., and Marcal, H. (2015). Chitosan as a biomaterial: Influence of degree of deacetylation on its physiochemical, material and biological properties. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0135153"},{"key":"ref_28","unstructured":"(2021, June 06). American Standard Test Method, ASTM International. Available online: http:\/\/www.astm.org."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/j.foodchem.2018.06.140","article-title":"Essential oils encapsulated in polymer-based nanocapsules as potential candidates for application in food preservation","volume":"269","author":"Granata","year":"2018","journal-title":"Food Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/0003-9861(78)90039-5","article-title":"Biosynthesis of aromatic monoterpenes: Conversion of \u03b3-terpinene to p-cymene and thymol in Thymus vulgaris L.","volume":"187","author":"Poulose","year":"1978","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_31","first-page":"4","article-title":"Study of quantitative and qualitative variations in essential oils of sicilian oregano biotypes","volume":"27","author":"Tuttolomondo","year":"2015","journal-title":"J. Essent. Oil Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1080\/10412905.2014.882278","article-title":"Essential oil characteristics of wild Sicilian oregano population in relation to environmental conditions","volume":"26","author":"Tuttolomondo","year":"2014","journal-title":"J. Essent. Oil Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1016\/j.bse.2010.08.008","article-title":"Screening of the essential oil composition of wild Sicilian thyme","volume":"38","author":"Napoli","year":"2010","journal-title":"Biochem. Syst. Ecol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2856","DOI":"10.1021\/mp300162j","article-title":"Insight on the formation of chitosan nanoparticles through ionotropic gelation with tripolyphosphate","volume":"9","author":"Koukaras","year":"2012","journal-title":"Mol. Pharm."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.colsurfb.2005.06.001","article-title":"Modulation of surface charge, particle size and morphological properties of chitosan\u2013TPP nanoparticles intended for gene delivery","volume":"44","author":"Gan","year":"2005","journal-title":"Colloids Surf. B"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1007\/12_2011_117","article-title":"Chitosan and its derivatives for drug delivery perspective","volume":"243","author":"Sonia","year":"2011","journal-title":"Adv. Polym. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Danaei, M., Dehghankhold, M., Ataei, S., Hasanzadeh Davarani, F., Javanmard, R., Dokhani, A., Khorasani, S., and Mozafari, M.R. (2018). Impact of particle size and polydispersity index on the clinical applications of lipidic nanocarrier systems. Pharmaceutics, 10.","DOI":"10.3390\/pharmaceutics10020057"},{"key":"ref_38","first-page":"914","article-title":"Characterization and optimization of colon targeted S-adenosyl-L-methionine loaded chitosan nanoparticles","volume":"23","author":"Ergin","year":"2019","journal-title":"J. Pharm. Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1211\/0022357023691","article-title":"Nanosuspensions: A promising drug delivery strategy","volume":"56","author":"Patravale","year":"2004","journal-title":"J. Pharm. Pharmacol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1016\/j.ijpharm.2007.11.052","article-title":"Preparation, characterization and antibacterial activities of chitosan, N-trimethyl chitosan (TMC) and N-diethylmethyl chitosan (DEMC) nanoparticles loaded with insulin using both the ionotropic gelation and polyelectrolyte complexation methods","volume":"355","author":"Sadeghi","year":"2008","journal-title":"Int. J. Pharm."},{"key":"ref_41","unstructured":"Jafari, S.M. (2020). Rheological Analysis of Solid-Like Nanoencapsulated Food Ingredients by Rheometers. Characterization of Nanoencapsulated Food Ingredients in Nanoencapsulation in the Food Industry, Academic Press."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1016\/j.ijbiomac.2017.05.063","article-title":"Release study and inhibitory activity of thyme essential oil-loaded chitosan nanoparticles and nanocapsules against foodborne bacteria","volume":"103","year":"2017","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.carbpol.2013.02.031","article-title":"Two-step method for encapsulation of oregano essential oil in chitosan nanoparticles: Preparation, characterization and in vitro release study","volume":"95","author":"Hosseini","year":"2013","journal-title":"Carbohydr. Polym."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1016\/j.foodchem.2017.10.106","article-title":"Hydroxycinnamic acids loaded in lipid-core nanocapsules","volume":"245","author":"Granata","year":"2018","journal-title":"Food Chem."},{"key":"ref_45","first-page":"589","article-title":"Characterization of chitosan nanoparticles added with essential oils. In vitro effect on Pectobacterium carotovorum","volume":"14","year":"2015","journal-title":"Rev. Mex. Ing. Qu\u00edm."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1111\/j.1472-765X.2006.01938.x","article-title":"Antimicrobial activity of carvacrol related to its chemical structure","volume":"43","author":"Combes","year":"2006","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"12","DOI":"10.3389\/fmicb.2012.00012","article-title":"Essential oils in food preservation: Mode of action, synergies, and interactions with food matrix components","volume":"3","author":"Hyldgaard","year":"2012","journal-title":"Front. Microbiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1111\/j.1472-765X.2008.02407.x","article-title":"The antibacterial mechanism of carvacrol and thymol against Escherichia coli","volume":"47","author":"Xu","year":"2008","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_49","first-page":"5","article-title":"Determination of degree of deacetylation of chitosan-comparision of methods","volume":"17","author":"Rokita","year":"2012","journal-title":"Prog. Chem. Appl. Chitin Deriv."},{"key":"ref_50","unstructured":"Adams, R.P. (2012). Identification of Essential Oil Components by Gas Chromatographic\/Quadrupole Mass Spectrometry, Allured Publishing Corporation. [4th ed.]."},{"key":"ref_51","unstructured":"Wayne, P.A. (2013). M100\u2013S23: Performance Standards for Antimicrobial Susceptibility Testing. Twenty-Third Informational Supplement, Clinical and Laboratory Standards Institute."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/26\/13\/4055\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:25:07Z","timestamp":1760163907000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/26\/13\/4055"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7,2]]},"references-count":51,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2021,7]]}},"alternative-id":["molecules26134055"],"URL":"https:\/\/doi.org\/10.3390\/molecules26134055","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,7,2]]}}}