{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T05:58:05Z","timestamp":1782626285641,"version":"3.54.5"},"reference-count":21,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2021,7,10]],"date-time":"2021-07-10T00:00:00Z","timestamp":1625875200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000313","name":"British Society for Antimicrobial Chemotherapy","doi-asserted-by":"publisher","award":["GA2016_020P"],"award-info":[{"award-number":["GA2016_020P"]}],"id":[{"id":"10.13039\/501100000313","id-type":"DOI","asserted-by":"publisher"}]},{"name":"universit\u00e9 de Tours","award":["Annual endowment"],"award-info":[{"award-number":["Annual endowment"]}]},{"DOI":"10.13039\/501100009469","name":"Conseil R\u00e9gional du Centre-Val de Loire","doi-asserted-by":"publisher","award":["PhD fellowship"],"award-info":[{"award-number":["PhD fellowship"]}],"id":[{"id":"10.13039\/501100009469","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Treatments currently used to prevent congenital toxoplasmosis are non-specific of Toxoplasma gondii and have grievous side effects. To develop a more specific and less toxic drug, we have designed SP230, an imidazo[1,2-b]pyridazine salt targeting the Toxoplasma gondii calcium-dependent protein kinase 1 (TgCDPK1) and active against acute toxoplasmosis in mice. Efficiency of SP230 to inhibit foetal transmission of the parasite was evaluated in a mouse model of congenital toxoplasmosis. Swiss mice were infected at mid-pregnancy with tachyzoites or cysts of the ME49 strain of T. gondii by intraperitoneal and oral route, respectively, and treated with SP230 at 50 mg\/kg for 5 days by the same routes. Parasite burden in organs of dams and in foetuses was measured by quantitative PCR. Intraperitoneal administration of SP230 drastically reduced the number of parasites (more than 97% of reduction) in the brain and lungs of dams, and led to a reduction of 66% of parasite burden in foetuses. Oral administration of SP230 was particularly efficient with 97% of reduction of parasite burdens in foetuses. SP230 did not impact number and weight of offspring in our conditions. This inhibitor of TgCDPK1 is a promising candidate for the development of alternative therapeutics to treat infected pregnant women.<\/jats:p>","DOI":"10.3390\/molecules26144203","type":"journal-article","created":{"date-parts":[[2021,7,12]],"date-time":"2021-07-12T00:23:36Z","timestamp":1626049416000},"page":"4203","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["A Novel Calcium-Dependent Protein Kinase 1 Inhibitor Potently Prevents Toxoplasma gondii Transmission to Foetuses in Mouse"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8300-6226","authenticated-orcid":false,"given":"H\u00e9lo\u00efse","family":"D\u00e9bare","sequence":"first","affiliation":[{"name":"Infectiologie et Sant\u00e9 Publique, National Research Institute for Agriculture, Food and the Environment, Universit\u00e9 de Tours, 37000 Tours, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Moir\u00e9","sequence":"additional","affiliation":[{"name":"Infectiologie et Sant\u00e9 Publique, National Research Institute for Agriculture, Food and the Environment, Universit\u00e9 de Tours, 37000 Tours, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6199-0894","authenticated-orcid":false,"given":"Firmin","family":"Baron","sequence":"additional","affiliation":[{"name":"Infectiologie et Sant\u00e9 Publique, National Research Institute for Agriculture, Food and the Environment, Universit\u00e9 de Tours, 37000 Tours, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3642-7500","authenticated-orcid":false,"given":"Louis","family":"Lantier","sequence":"additional","affiliation":[{"name":"Infectiologie et Sant\u00e9 Publique, National Research Institute for Agriculture, Food and the Environment, Universit\u00e9 de Tours, 37000 Tours, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"H\u00e9raut","sequence":"additional","affiliation":[{"name":"Infectiologie et Sant\u00e9 Publique, National Research Institute for Agriculture, Food and the Environment, Universit\u00e9 de Tours, 37000 Tours, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Van Langendonck","sequence":"additional","affiliation":[{"name":"Service de Parasitologie-Mycologie-M\u00e9decine Tropicale, Centre Hospitalier R\u00e9gional Universitaire de Tours, 37000 Tours, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Denevault-Sabourin","sequence":"additional","affiliation":[{"name":"Groupe Innovation et Ciblage Cellulaire EA7501, Universit\u00e9 de Tours, 37200 Tours, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Dimier-Poisson","sequence":"additional","affiliation":[{"name":"Infectiologie et Sant\u00e9 Publique, National Research Institute for Agriculture, Food and the Environment, Universit\u00e9 de Tours, 37000 Tours, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7050-3891","authenticated-orcid":false,"given":"Fran\u00e7oise","family":"Debierre-Grockiego","sequence":"additional","affiliation":[{"name":"Infectiologie et Sant\u00e9 Publique, National Research Institute for Agriculture, Food and the Environment, Universit\u00e9 de Tours, 37000 Tours, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,7,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1016\/j.parint.2018.07.004","article-title":"Congenital toxoplasmosis: An overview of the neurological and ocular manifestations","volume":"67","author":"Khan","year":"2018","journal-title":"Parasitol. 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