{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T14:18:09Z","timestamp":1790691489466,"version":"4.1.0"},"reference-count":110,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2021,7,31]],"date-time":"2021-07-31T00:00:00Z","timestamp":1627689600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81803686"],"award-info":[{"award-number":["81803686"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Supporting Project of National Natural Science Foundation of China","award":["2018PP01"],"award-info":[{"award-number":["2018PP01"]}]},{"name":"Cooperative Scientific Research Project of \u2018Chunhui Plan\u2019 of Ministry of Education","award":["HLJ2019035"],"award-info":[{"award-number":["HLJ2019035"]}]},{"name":"University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province","award":["UNPYSCT-2020225"],"award-info":[{"award-number":["UNPYSCT-2020225"]}]},{"name":"Special Fund Project of Postdoctor in Heilongjiang Province","award":["LBH-Q20180"],"award-info":[{"award-number":["LBH-Q20180"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Thin-layer chromatography (TLC) bioautography is an evolving technology that integrates the separation and analysis technology of TLC with biological activity detection technology, which has shown a steep rise in popularity over the past few decades. It connects TLC with convenient, economic and intuitive features and bioautography with high levels of sensitivity and specificity. In this study, we discuss the research progress of TLC bioautography and then establish a definite timeline to introduce it. This review summarizes known TLC bioautography types and practical applications for determining antibacterial, antifungal, antitumor and antioxidant compounds and for inhibiting glucosidase, pancreatic lipase, tyrosinase and cholinesterase activity constitutes. Nowadays, especially during the COVID-19 pandemic, it is important to identify original, natural products with anti-COVID potential compounds from Chinese traditional medicine and natural medicinal plants. We also give an account of detection techniques, including in situ and ex situ techniques; even in situ ion sources represent a major reform. Considering the current technical innovations, we propose that the technology will make more progress in TLC plates with higher separation and detection technology with a more portable and extensive scope of application. We believe this technology will be diffusely applied in medicine, biology, agriculture, animal husbandry, garden forestry, environmental management and other fields in the future.<\/jats:p>","DOI":"10.3390\/molecules26154647","type":"journal-article","created":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T21:44:32Z","timestamp":1627854272000},"page":"4647","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":50,"title":["An Evolving Technology That Integrates Classical Methods with Continuous Technological Developments: Thin-Layer Chromatography Bioautography"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-5784-2598","authenticated-orcid":false,"given":"Meng","family":"Wang","sequence":"first","affiliation":[{"name":"Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin 150000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yirong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin 150000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruijie","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin 150000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhibin","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin 150000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bingyou","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin 150000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haixue","family":"Kuang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin 150000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,7,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1002\/pca.2669","article-title":"Recent Advances in Effect-directed Enzyme Assays based on Thin-layer Chromatography","volume":"28","author":"Wolfram","year":"2017","journal-title":"Phytochem. 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