{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T02:14:27Z","timestamp":1760235267807,"version":"build-2065373602"},"reference-count":46,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2021,8,4]],"date-time":"2021-08-04T00:00:00Z","timestamp":1628035200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["1723\/2018-00  PROCAD-AMAZONIA"],"award-info":[{"award-number":["1723\/2018-00  PROCAD-AMAZONIA"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["Proc.402332\/2013-0"],"award-info":[{"award-number":["Proc.402332\/2013-0"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>This study aimed to evaluate and compare the effects of co-treatment with purified annatto oil (PAO) or its granules (GRA, Chronic\u00ae) with that of testosterone on the orchiectomy-induced osteoporosis in Wistar rats. After surgery, rats were treated from day 7 until day 45 with testosterone only (TES, 7 mg\/kg, IM) or TES + PAO or GRA (200 mg\/kg, p.o.). The following parameters were evaluated: food\/water intake, weight, HDL, LDL, glucose, triglycerides (TG), total cholesterol (TC), alkaline phosphatase levels, blood phosphorus and calcium contents, femur weight, structure (through scanning electron microscopy), and calcium content (through atomic absorption spectrophotometry). Our results show that orchiectomy could significantly change the blood lipid profile and decrease bone integrity parameters. Testosterone reposition alone could improve some endpoints, including LDL, TC, bone weight, and bone calcium concentration. However, other parameters were not significantly improved. Co-treatment with PAO or GRA improved the blood lipid profile and bone integrity more significantly and improved some endpoints not affected by testosterone reposition alone (such as TG levels and trabeculae sizes). The results suggest that co-treatment with annatto products improved the blood lipid profile and the anti-osteoporosis effects of testosterone. Overall, GRA had better results than PAO.<\/jats:p>","DOI":"10.3390\/molecules26164720","type":"journal-article","created":{"date-parts":[[2021,8,4]],"date-time":"2021-08-04T08:47:52Z","timestamp":1628066872000},"page":"4720","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Co-Treatment of Purified Annatto Oil (Bixa orellana L.) and Its Granules (Chronic\u00ae) Improves the Blood Lipid Profile and Bone Protective Effects of Testosterone in the Orchiectomy-Induced Osteoporosis in Wistar Rats"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9504-5081","authenticated-orcid":false,"given":"Arlindo C\u00e9sar Matias","family":"Pereira","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa em F\u00e1rmacos, Departamento de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade, Colegiado de Farm\u00e1cia, Universidade Federal do Amap\u00e1, Macap\u00e1 68902-280, AP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helison","family":"de Oliveira Carvalho","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa em F\u00e1rmacos, Departamento de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade, Colegiado de Farm\u00e1cia, Universidade Federal do Amap\u00e1, Macap\u00e1 68902-280, AP, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Inova\u00e7\u00e3o Farmac\u00eautica, Departamento de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade, Colegiado de Farm\u00e1cia, Universidade Federal do Amap\u00e1, Macap\u00e1 68902-280, AP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danna Emanuelle Santos","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa em F\u00e1rmacos, Departamento de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade, Colegiado de Farm\u00e1cia, Universidade Federal do Amap\u00e1, Macap\u00e1 68902-280, AP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karyny Roberta Tavares","family":"Pican\u00e7o","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa em F\u00e1rmacos, Departamento de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade, Colegiado de Farm\u00e1cia, Universidade Federal do Amap\u00e1, Macap\u00e1 68902-280, AP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1783-3861","authenticated-orcid":false,"given":"Abrah\u00e3o Victor Tavares","family":"de Lima Teixeira dos Santos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa em F\u00e1rmacos, Departamento de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade, Colegiado de Farm\u00e1cia, Universidade Federal do Amap\u00e1, Macap\u00e1 68902-280, AP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heitor Ribeiro","family":"da Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa em F\u00e1rmacos, Departamento de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade, Colegiado de Farm\u00e1cia, Universidade Federal do Amap\u00e1, Macap\u00e1 68902-280, AP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francinaldo Sarges","family":"Braga","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Absor\u00e7\u00e3o At\u00f4mica e Bioprospec\u00e7\u00e3o, Departamento de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade, Colegiado de Farm\u00e1cia, Universidade Federal do Amap\u00e1, Macap\u00e1 68902-280, AP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1836-4246","authenticated-orcid":false,"given":"Roberto Messias","family":"Bezerra","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Absor\u00e7\u00e3o At\u00f4mica e Bioprospec\u00e7\u00e3o, Departamento de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade, Colegiado de Farm\u00e1cia, Universidade Federal do Amap\u00e1, Macap\u00e1 68902-280, AP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandro","family":"de Sousa Nunes","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa em F\u00e1rmacos, Departamento de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade, Colegiado de Farm\u00e1cia, Universidade Federal do Amap\u00e1, Macap\u00e1 68902-280, AP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maira Tiyomi Sacata Tongo","family":"Nazima","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa em F\u00e1rmacos, Departamento de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade, Colegiado de Farm\u00e1cia, Universidade Federal do Amap\u00e1, Macap\u00e1 68902-280, AP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00falia Gomes","family":"Cerqueira","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Talisson","family":"Taglialegna","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janayra Maris","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa em F\u00e1rmacos, Departamento de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade, Colegiado de Farm\u00e1cia, Universidade Federal do Amap\u00e1, Macap\u00e1 68902-280, AP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3662-9794","authenticated-orcid":false,"given":"Jos\u00e9 Carlos 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