{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T16:37:26Z","timestamp":1781195846315,"version":"3.54.1"},"reference-count":32,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2021,9,13]],"date-time":"2021-09-13T00:00:00Z","timestamp":1631491200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Previous studies of Ganoderma lucidum have focused on its medicinal applications. Limited information is available about its antibacterial activity against plant pathogens. Thus, the goal of this study was to purify and characterize the antibacterial activity against plant pathogenic bacteria from culture fluids of G. lucidum. The nature of the bioactive components was determined using heat boiling, organic solvents, dialysis tubing, gel exclusion chromatography (GEC), proteinase sensitivity, HPLC, HPLC-APCI-MS, and GC-MS. The bioactive compounds were neither lipid, based on their solubility, nor proteic in nature, based on proteinase digestion and heat stability. The putative-bioactive polysaccharides have molecular weights that range from 3500 to 4500 Daltons as determined by dialysis tubing, GEC and APCI-MS analysis. The composition of the antibacterial compounds was determined by GC-MS. This is the first report of small polysaccharides produced by G. lucidum with activity against bacterial plant pathogens.<\/jats:p>","DOI":"10.3390\/molecules26185553","type":"journal-article","created":{"date-parts":[[2021,9,13]],"date-time":"2021-09-13T23:32:23Z","timestamp":1631575943000},"page":"5553","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Purification and Characterization of Antibacterial Activity against Phytopathogenic Bacteria in Culture Fluids from Ganoderma lucidum"],"prefix":"10.3390","volume":"26","author":[{"given":"Loreto","family":"Robles-Hern\u00e1ndez","sequence":"first","affiliation":[{"name":"Facultad de Ciencias Agrotecnol\u00f3gicas, Universidad Aut\u00f3noma de Chihuahua, Ciudad Universitaria S\/N Campus 1, Chihuahua 31310, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8002-4878","authenticated-orcid":false,"given":"Nora A.","family":"Salas-Salazar","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Agrotecnol\u00f3gicas, Universidad Aut\u00f3noma de Chihuahua, Ciudad Universitaria S\/N Campus 1, Chihuahua 31310, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana C.","family":"Gonzalez-Franco","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Agrotecnol\u00f3gicas, Universidad Aut\u00f3noma de Chihuahua, Ciudad Universitaria S\/N Campus 1, Chihuahua 31310, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,9,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/S0031-9422(01)00450-2","article-title":"Structural features of immunologically active polysaccharides from Ganoderma lucidum","volume":"59","author":"Bao","year":"2002","journal-title":"Phytochemistry"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1016\/S0142-9612(97)00048-3","article-title":"Fungal mycelia as the source of chitin and polysaccharides and their applications as skin substitutes","volume":"18","author":"Su","year":"1997","journal-title":"Biomaterials"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1006\/bbrc.1998.9175","article-title":"Ligand binding to the (1-->3) beta-D-glucan receptor stimulates NFkappaB activation, but not apoptosis in U937 cells","volume":"249","author":"Battle","year":"1998","journal-title":"Biochem. 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