{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T02:09:35Z","timestamp":1783649375205,"version":"3.55.0"},"reference-count":73,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2021,9,15]],"date-time":"2021-09-15T00:00:00Z","timestamp":1631664000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Antimicrobial resistance is a dramatic global threat; however, the slow progress of new antibiotic development has impeded the identification of viable alternative strategies. Natural antioxidant-based antibacterial approaches may provide potent therapeutic abilities to effectively block resistance microbes\u2019 pathways. While essential oils (EOs) have been reported as antimicrobial agents, its application is still limited ascribed to its low solubility and stability characters; additionally, the related biomolecular mechanisms are not fully understood. Hence, the study aimed to develop a nano-gel natural preparation with multiple molecular mechanisms that could combat bacterial resistance in an acne vulgaris model. A nano-emulgel of thyme\/clove EOs (NEG8) was designed, standardized, and its antimicrobial activity was screened in vitro and in vivo against genetically identified skin bacterial clinical isolates (Pseudomonas stutzeri, Enterococcus faecium and Bacillus thuringiensis). As per our findings, NEG8 exhibited bacteriostatic and potent biofilm inhibition activities. An in vivo model was also established using the commercially available therapeutic, adapalene in contra genetically identified microorganism. Improvement in rat behavior was reported for the first time and NEG8 abated the dermal contents\/protein expression of IGF-1, TGF-\u03b2\/collagen, Wnt\/\u03b2-catenin, JAK2\/STAT-3, NE, 5-HT, and the inflammatory markers; p(Ser536) NF-\u03baBp65, TLR-2, and IL-6. Moreover, the level of dopamine, protective anti-inflammatory cytokine, IL-10 and PPAR-\u03b3 protein were enhanced, also the skin histological structures were improved. Thus, NEG8 could be a future potential topical clinical alternate to synthetic agents, with dual merit mechanism as bacteriostatic antibiotic action and non-antibiotic microbial pathway inhibitor.<\/jats:p>","DOI":"10.3390\/molecules26185608","type":"journal-article","created":{"date-parts":[[2021,9,15]],"date-time":"2021-09-15T21:47:11Z","timestamp":1631742431000},"page":"5608","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Topical Nano Clove\/Thyme Gel against Genetically Identified Clinical Skin Isolates: In Vivo Targeting Behavioral Alteration and IGF-1\/pFOXO-1\/PPAR \u03b3 Cues"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1149-8222","authenticated-orcid":false,"given":"Jilan A.","family":"Nazeam","sequence":"first","affiliation":[{"name":"Pharmacognosy Department, Faculty of Pharmacy, October 6 University, Giza 12585, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ghada M.","family":"Ragab","sequence":"additional","affiliation":[{"name":"Pharmacology and Toxicological Department, Faculty of Pharmacy, Misr University, Giza 12585, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8372-4682","authenticated-orcid":false,"given":"Amira A.","family":"El-Gazar","sequence":"additional","affiliation":[{"name":"Pharmacology and Toxicological Department, Faculty of Pharmacy, October 6 University, Giza 12585, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8151-6778","authenticated-orcid":false,"given":"Shereen S.","family":"El-Mancy","sequence":"additional","affiliation":[{"name":"Pharmaceutics Department, Faculty of Pharmacy, October 6 University, Giza 12585, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lina","family":"Jamil","sequence":"additional","affiliation":[{"name":"Microbiology and Immunology Department, Faculty of Pharmacy, October 6 University, Giza 12585, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0001-6147","authenticated-orcid":false,"given":"Sahar M.","family":"Fayez","sequence":"additional","affiliation":[{"name":"Pharmaceutics Department, Faculty of Pharmacy, October 6 University, Giza 12585, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,9,15]]},"reference":[{"key":"ref_1","unstructured":"Ledingham, K., Hinchliffe, S., Jackson, M., Thomas, F., Tomson, G., and World Health Organization (2021, September 06). Antibiotic Resistance: Using a Cultural Contexts of Health Approach to Address a Global Health Challenge. Available online: https:\/\/apps.who.int\/iris\/handle\/10665\/330029."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1669","DOI":"10.3389\/fmicb.2020.01669","article-title":"The demand for new antibiotics: Antimicrobial peptides, nanoparticles, and combinatorial therapies as future strategies in antibacterial agent design","volume":"11","year":"2020","journal-title":"Front Microbiol."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Lagadinou, M., Onisor, M.O., Rigas, A., Musetescu, D.-V., Gkentzi, D., Assimakopoulos, S.F., Panos, G., and Marangos, M. (2020). Antimicrobial properties on non-antibiotic drugs in the era of increased bacterial resistance. Antibiotics, 9.","DOI":"10.3390\/antibiotics9030107"},{"key":"ref_4","first-page":"133","article-title":"Antibiotic development and the changing role of the pharmaceutical industry","volume":"1","author":"Monnet","year":"2005","journal-title":"Int. J. Risk Saf. Med."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Saleem, M., Nazir, M., Ali, M.S., Hussain, H., Lee, Y.S., Riaz, N., and Jabbar, A. (2010). ChemInform Abstract: Antimicrobial natural products: An update on future antibiotic drug candidates. ChemInform, 41.","DOI":"10.1002\/chin.201021265"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Naqvi, S.A., Sana, N., Sana, K., Syed, A.N., Muhammad, S.M., Sajid, Y.Q., and Shahzad, A. (2019). Antioxidants: Natural antibiotics. Antioxidants, IntechOpen.","DOI":"10.5772\/intechopen.84864"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"142","DOI":"10.2478\/hepo-2013-0030","article-title":"Application of essential oils as natural cosmetic preservatives","volume":"59","author":"Dreger","year":"2013","journal-title":"Herba Pol."},{"key":"ref_8","first-page":"100013","article-title":"Formulation, and development of lipid nanoparticle antifungal packaging films to control postharvest disease","volume":"1","author":"McDaniel","year":"2019","journal-title":"J. Agric. Food Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2014\/651593","article-title":"Essential oils loaded in nanosystems: A developing strategy for a successful therapeutic approach","volume":"2014","author":"Bilia","year":"2014","journal-title":"Evidence-Based Complement. Altern. Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2545","DOI":"10.1056\/NEJMoa1607033","article-title":"A placebo-controlled trial of antibiotics for smaller skin abscesses","volume":"376","author":"Daum","year":"2017","journal-title":"N. Engl. J. Med."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.arr.2003.08.003","article-title":"Skin infections and ageing","volume":"3","author":"Laube","year":"2004","journal-title":"Ageing Res. Rev."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"122","DOI":"10.4103\/0019-5154.43213","article-title":"Bacterial resistance to antibiotics in acne vulgaris: An in vitro study","volume":"53","author":"Hassanzadeh","year":"2008","journal-title":"Indian J. Dermatol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1007\/s13555-018-0224-7","article-title":"A Qualitative investigation of the impact of Acne on health-related quality of life (HRQL): Development of a conceptual model","volume":"8","author":"Fabbrocini","year":"2018","journal-title":"Dermatol. Ther."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/S1473-3099(15)00527-7","article-title":"Systematic review of antibiotic resistance in acne: An increasing topical and oral threat","volume":"16","author":"Walsh","year":"2016","journal-title":"Lancet Infect. Dis."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/S0140-6736(11)60321-8","article-title":"Acne vulgaris","volume":"28","author":"Williams","year":"2012","journal-title":"Lancet"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Bueno, J., Demirci, F., and Baser, K.H.C. (2017). Essential oils against microbial resistance mechanisms, challenges, and applications in drug discovery. Essential Oils and Nanotechnology for Treatment of Microbial Diseases, CRC Press.","DOI":"10.1201\/9781315209241-6"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"6","DOI":"10.2174\/1874285801408010006","article-title":"Essential oils, a new horizon in combating bacterial antibiotic resistance","volume":"8","author":"Yap","year":"2014","journal-title":"Open Microbiol. J."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/S0023-6438(95)80008-5","article-title":"Use of a free radical method to evaluate antioxidant activity","volume":"28","author":"Cuvelier","year":"1995","journal-title":"LWT"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2083","DOI":"10.1007\/s00706-016-1837-0","article-title":"Essential oils as antioxidants: Their evaluation by DPPH, ABTS, FRAP, CUPRAC, and \u03b2-carotene bleaching methods","volume":"147","author":"Olszowy","year":"2016","journal-title":"Monatsh. Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.foodchem.2017.11.032","article-title":"Thymol nanoemulsions incorporated in quinoa protein\/chitosan edible films; antifungal effect in cherry tomatoes","volume":"246","author":"Robledo","year":"2018","journal-title":"Food Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1038\/176510b0","article-title":"Theory of antibiotic inhibition zones in agar media","volume":"176","author":"Cooper","year":"1955","journal-title":"Nature"},{"key":"ref_22","unstructured":"Gehardt, P., Murray, E., and Costilow, N. (1981). Manual of Methods for General Biotechnology, American society for microbiology."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2052","DOI":"10.1128\/AEM.70.4.2052-2060.2004","article-title":"Identification of the bacterial community of Maple sap by using amplified ribosomal DNA (rDNA) restriction analysis and rDNA sequencing","volume":"70","author":"Pitre","year":"2004","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"Altschul","year":"1990","journal-title":"J. Mol. Biol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"4673","DOI":"10.1093\/nar\/22.22.4673","article-title":"Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice","volume":"11","author":"Thompson","year":"1994","journal-title":"Nucleic Acids Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1002\/path.1700580110","article-title":"The diffusion of antiseptics through agar gels, with special reference to the agar cup assay method of estimating the activity of penicillin","volume":"58","author":"Cooper","year":"1946","journal-title":"J. Pathol. Bacteriol."},{"key":"ref_27","first-page":"21","article-title":"Antibacterial, antifungal activity and phytochemical analysis of Sanseviveria roxburghiana root","volume":"3","author":"Moideen","year":"2012","journal-title":"Int. J. Phytopharmacol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1111\/j.1600-0463.2007.apm_630.x","article-title":"Quantification of biofilm in microtiter plates: Overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci","volume":"115","author":"Vukovic","year":"2007","journal-title":"APMIS"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.mimet.2010.08.018","article-title":"In vitro and in vivo model systems to study microbial biofilm formation","volume":"83","author":"Coenye","year":"2010","journal-title":"J. Microbiol. Methods"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.peptides.2017.11.001","article-title":"The antimicrobial effect of CEN1HC-Br against Propionibacterium acnes and its therapeutic and anti-inflammatory effects on acne vulgaris","volume":"1","author":"Han","year":"2018","journal-title":"Peptides"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"101418","DOI":"10.1016\/j.jddst.2019.101418","article-title":"Exploring therapeutic potential of azelaic acid loaded NLCs for the treatment of acne vulgaris","volume":"55","author":"Malik","year":"2019","journal-title":"J. Drug Deliv. Sci. Technol."},{"key":"ref_32","first-page":"598","article-title":"Anti-amnestic properties of Ginkgo biloba extract on impaired memory function induced by aluminum in rats","volume":"31","author":"Elsheikh","year":"2012","journal-title":"Int. J. Dev. Neurosci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"103329","DOI":"10.1016\/j.etap.2020.103329","article-title":"Analgesic and anti-inflammatory effects and mechanism of action of borneol on photodynamic therapy of acne","volume":"75","author":"Ji","year":"2020","journal-title":"Environ. Toxicol. Pharmacol."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Sarkic, A., and Stappen, I. (2018). Essential oils and their single compounds in cosmetics\u2014A critical review. Cosmetics, 5.","DOI":"10.3390\/cosmetics5010011"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"989","DOI":"10.1002\/ptr.2179","article-title":"Antimicrobial activity of clove and rosemary essential oils alone and in combination","volume":"21","author":"Fu","year":"2007","journal-title":"Phytother. Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1688","DOI":"10.1002\/ptr.6109","article-title":"Thymol, thyme, and other plant sources: Health and potential uses","volume":"32","author":"Salehi","year":"2018","journal-title":"Phytother Res."},{"key":"ref_37","first-page":"S875","article-title":"Antioxidant activity of the essential oil and its major terpenes of Satureja macrostema (Moc. and Sess\u00e9 ex Benth.) Briq","volume":"13","author":"Garciglia","year":"2018","journal-title":"Pharmacogn. Mag."},{"key":"ref_38","first-page":"338","article-title":"Evaluation of burn wound healing activity of novel fusidic acid loaded microemulsion based gel in male wistar albino rats. Saudi","volume":"1","author":"Okur","year":"2020","journal-title":"Pharm. J."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"781","DOI":"10.4172\/pharmaceutical-sciences.1000422","article-title":"Review of nanoemulsion formulation and characterization techniques","volume":"80","author":"Gurpret","year":"2018","journal-title":"Indian J. Pharm. Sci."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"18","DOI":"10.20510\/ukjpb\/3\/i1\/89220","article-title":"A comparative study of the antibacterial activity of clove and rosemary essential oils on multidrug-resistant bacteria. UK","volume":"3","author":"Abdullah","year":"2015","journal-title":"J. Pharm. Biosci."},{"key":"ref_41","first-page":"33","article-title":"Antimicrobial activities of clove and thyme extracts","volume":"6","author":"Nzeako","year":"2006","journal-title":"Sultan Qaboos Univ. Med. J."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Miklasi\u0144ska-Majdanik, M., K\u0119pa, M., Wojtyczka, R.D., Idzik, D., and W\u0105sik, T.J. (2018). Phenolic compounds diminish antibiotic resistance of Staphylococcus aureus clinical strains. Int. J. Environ. Res. Public Health, 15.","DOI":"10.3390\/ijerph15102321"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1106","DOI":"10.3389\/fmicb.2021.676458","article-title":"Bacterial biofilm inhibition: A focused review on recent therapeutic strategies for combating the biofilm mediated infections","volume":"12","author":"Srinivasan","year":"2021","journal-title":"Front. Microbiol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1016\/j.jgar.2019.11.012","article-title":"Non-antibiotic antimicrobial agents to combat bio-film-forming bacteria","volume":"21","author":"Cao","year":"2020","journal-title":"J. Glob. Antimicrob. Resist."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1007\/s00403-019-01908-x","article-title":"From pathogenesis of acne vulgaris to anti-acne agents","volume":"311","author":"Cong","year":"2019","journal-title":"Arch. Dermatol. Res."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"130","DOI":"10.4161\/derm.15025","article-title":"Peroxisome proliferator-activated receptors (PPARs) in dermatology: Challenge and promise","volume":"1","author":"Sertznig","year":"2011","journal-title":"Dermato-Endocrinol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2002","DOI":"10.1038\/sj.jid.5700336","article-title":"Peroxisome prolifera-tor-activated receptors increase human sebum production","volume":"1","author":"Trivedi","year":"2006","journal-title":"J. Investig. Dermatol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"12188","DOI":"10.1074\/jbc.M808915200","article-title":"FOXO1 transrepresses peroxisome prolifera-tor-activated receptor \u03b3 transactivation, coordinating an insulin-induced feed-forward response in adipocytes","volume":"284","author":"Fan","year":"2009","journal-title":"J. Biol. Chem."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1016\/S0003-9861(02)00207-2","article-title":"Regulation of the FoxO family of transcription factors by phosphatidylinositol-3 ki-nase-activated signaling","volume":"403","author":"Arden","year":"2002","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1038\/jid.2013.334","article-title":"Propionibacterium acnes induces an IL-17 response in Acne vulgaris that is regulated by vitamin A and vitamin D","volume":"134","author":"Agak","year":"2014","journal-title":"J. Investig. Dermatol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1582","DOI":"10.1016\/j.bbi.2011.05.007","article-title":"Early and adult hippocampal TGF-\u03b21 overexpression have opposite effects on behavior","volume":"25","author":"Depino","year":"2011","journal-title":"Brain Behav. Immun."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1146\/annurev.cellbio.20.010403.113126","article-title":"The WNT signaling pathway in development and disease","volume":"20","author":"Logan","year":"2004","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"ref_53","first-page":"90579","article-title":"Interactions between TGF-\u03b21, canonical WNT\/\u03b2-catenin pathway and PPAR \u03b3 in radiation-induced fibrosis","volume":"27","author":"Lecarpentier","year":"2017","journal-title":"Oncotarget"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1016\/j.immuni.2008.03.002","article-title":"Cytokine signaling modules in inflammatory responses","volume":"28","author":"Murray","year":"2008","journal-title":"Immunity"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1002\/ptr.3288","article-title":"The potential use of Echinacea in acne: Control of Propionibacterium acnes growth and inflammation","volume":"25","author":"Sharma","year":"2010","journal-title":"Phytother. Res."},{"key":"ref_56","first-page":"734","article-title":"Diagnosis and treatment of acne","volume":"86","author":"Titus","year":"2012","journal-title":"Am. Fam. Physician"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1535","DOI":"10.4049\/jimmunol.169.3.1535","article-title":"Activation of toll-like receptor 2 in acne triggers inflammatory cytokine responses","volume":"1","author":"Kim","year":"2002","journal-title":"J. Immunol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1002\/ptr.2319","article-title":"Anti-acne activity of Selaginella involvens extract and its non-antibiotic antimicrobial potential on Propionibacterium acnes","volume":"22","author":"Joo","year":"2008","journal-title":"Phytotherapy Res."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"129753","DOI":"10.4061\/2011\/129753","article-title":"Glycogen synthase kinase-3\u03b2: A mediator of inflammation in Alzheimer\u2019s disease?","volume":"2011","author":"Koistinaho","year":"2011","journal-title":"Int. J. Alzheimers Dis."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1016\/0014-2999(68)90023-X","article-title":"Effects of stress on the metabolism of noradrenaline, dopamine and serotonin (5HT) in the central nervous system of the rat (II) modifications of serotonin metabolism","volume":"4","author":"Thierry","year":"1968","journal-title":"Eur. J. Pharmacol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1111\/j.1748-1716.1967.tb03728.x","article-title":"A Possible role played by central monoamine neurones in thermo-regulation","volume":"71","author":"Corrodi","year":"1967","journal-title":"Acta Physiol. Scand."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.it.2005.11.006","article-title":"Cytokines sing the blues: Inflammation and the pathogenesis of depression","volume":"27","author":"Raison","year":"2006","journal-title":"Trends Immunol."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Yap, P.S., Yang, S.K., Lai, K.S., and Lim, S.H. (2017). Essential oils: The ultimate solution to antimicrobial resistance in Escherichia coli. Escherichia coli: Recent Advances on Physiology, Pathogenesis and Biotechnological Applications, IntechOpen.","DOI":"10.5772\/67776"},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Yang, S.-K., Tan, N.-P., Chong, C.-W., Abushelaibi, A., Lim, S.-H., and Lai, K.-S. (2021). The missing piece: Recent approaches investigating the antimicrobial mode of action of essential oils. Evol. Bioinform., 17.","DOI":"10.1177\/1176934320938391"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"5145","DOI":"10.1016\/j.sjbs.2021.05.033","article-title":"Using essential oils to overcome bacterial biofilm formation and their antimicrobial resistance","volume":"28","author":"Alagawany","year":"2021","journal-title":"Saudi J. Biol. Sci."},{"key":"ref_66","first-page":"92","article-title":"Antimicrobial potential of Pectis substriata essential oil (Asteraceae) against drug-resistant Staphylococcus strains","volume":"14","author":"Jesus","year":"2020","journal-title":"An Acad Bras Cienc."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"784","DOI":"10.1111\/j.1365-4632.2012.05654.x","article-title":"A review of applications of tea tree oil in dermatology","volume":"52","author":"Pazyar","year":"2012","journal-title":"Int. J. Dermatol."},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Li, Z.-H., Cai, M., Liu, Y.-S., Sun, P.-L., and Luo, S.-L. (2019). Antibacterial activity, and mechanisms of essential oil from Citrus medica L.. var. sarcodactylis. Molecules, 24.","DOI":"10.3390\/molecules24081577"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"7329","DOI":"10.2147\/IJN.S265934","article-title":"Recent advances in nanotechnology-aided materials in combating microbial resistance and functioning as antibiotics substitutes","volume":"15","author":"Munir","year":"2020","journal-title":"Int. J. Nanomedicine."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.ijpharm.2017.01.013","article-title":"Synergistic antimicrobial potential of essential oils in combination with nanoparticles: Emerging trends and future perspectives","volume":"519","author":"Rai","year":"2017","journal-title":"Int. J. Pharm."},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"Taleb, M.H., Abdeltawab, N.F., Shamma, R.N., Abdelgayed, S.S., Mohamed, S.S., Farag, M.A., and Ramadan, M.A. (2018). Origanum vulgare L. essential oil as a potential anti-acne topical nanoemulsion\u2014In vitro and in vivo study. Molecules, 23.","DOI":"10.3390\/molecules23092164"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1580","DOI":"10.3389\/fmicb.2016.01580","article-title":"Encapsulation of Cardamom essential oil in chitosan nanocomposites: In-vitro Efficacy on antibiotic-resistant bacterial pathogens and cytotoxicity studies","volume":"7","author":"Jamil","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2016\/3012462","article-title":"Antimicrobial properties of plant essential oils against human pathogens and their mode of action: An updated review","volume":"2016","author":"Swamy","year":"2016","journal-title":"Evid. Based Complement. Altern. Med."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/26\/18\/5608\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:00:25Z","timestamp":1760166025000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/26\/18\/5608"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,15]]},"references-count":73,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2021,9]]}},"alternative-id":["molecules26185608"],"URL":"https:\/\/doi.org\/10.3390\/molecules26185608","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,9,15]]}}}