{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:58:12Z","timestamp":1787029092790,"version":"build-2736575974"},"reference-count":45,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2021,9,18]],"date-time":"2021-09-18T00:00:00Z","timestamp":1631923200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006507","name":"Funda\u00e7\u00e3o Oswaldo Cruz","doi-asserted-by":"publisher","award":["PAEF"],"award-info":[{"award-number":["PAEF"]}],"id":[{"id":"10.13039\/501100006507","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["302160\/2019-1"],"award-info":[{"award-number":["302160\/2019-1"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["400043\/2017-3"],"award-info":[{"award-number":["400043\/2017-3"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"publisher","award":["CNE"],"award-info":[{"award-number":["CNE"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Chagas disease (CD) affects more than 6 million people worldwide. The available treatment is far from ideal, creating a demand for new alternative therapies. Botanical diversity provides a wide range of novel potential therapeutic scaffolds. Presently, our aim was to evaluate the mammalian host toxicity and anti-Trypanosoma cruzi activity of botanic natural products including extracts, fractions and purified compounds obtained from Brazilian flora. In this study, 36 samples of extracts and fractions and eight pure compounds obtained from seven plant species were evaluated. The fraction dichloromethane from Aureliana fasciculata var. fasciculata (AFfPD) and the crude extract of Piper tectoniifolium (PTFrE) showed promising trypanosomicidal activity. AFfPD and PTFrE presented EC50 values 10.7 \u00b1 2.8 \u03bcg\/mL and 12.85 \u00b1 1.52 \u03bcg\/mL against intracellular forms (Tulahuen strain), respectively. Additionally, both were active upon bloodstream trypomastigotes (Y strain), exhibiting EC50 2.2 \u00b1 1.0 \u03bcg\/mL and 38.8 \u00b1 2.1 \u03bcg\/mL for AFfPD and PTFrE, respectively. Importantly, AFfPD is about five-fold more potent than Benznidazole (Bz), the reference drug for CD, also reaching lower EC90 value (7.92 \u00b1 2.2 \u03bcg\/mL) as compared to Bz (23.3 \u00b1 0.6 \u03bcg\/mL). Besides, anti-parasitic effect of eight purified botanic substances was also investigated. Aurelianolide A and B (compounds 1 and 2) from A. fasciculata and compound 8 from P. tuberculatum displayed the best trypanosomicidal effect. Compounds 1, 2 and 8 showed EC50 of 4.6 \u00b1 1.3 \u03bcM, 1.6 \u00b1 0.4 \u03bcM and 8.1 \u00b1 0.9 \u03bcM, respectively against intracellular forms. In addition, in silico analysis of these three biomolecules was performed to predict parameters of absorption, distribution, metabolism and excretion. The studied compounds presented similar ADMET profile as Bz, without presenting mutagenicity and hepatotoxicity aspects as predicted for Bz. Our findings indicate that these natural products have promising anti-T. cruzi effect and may represent new scaffolds for future lead optimization.<\/jats:p>","DOI":"10.3390\/molecules26185676","type":"journal-article","created":{"date-parts":[[2021,9,21]],"date-time":"2021-09-21T22:34:01Z","timestamp":1632263641000},"page":"5676","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["In Vitro Phenotypic Activity and In Silico Analysis of Natural Products from Brazilian Biodiversity on Trypanosoma cruzi"],"prefix":"10.3390","volume":"26","author":[{"given":"Raiza B.","family":"Peres","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Biologia Celular, Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz (FIOCRUZ), Avenida Brasil 4365, Manguinhos, Rio de Janeiro 210360-040, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ludmila F. de A.","family":"Fiuza","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Celular, Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz (FIOCRUZ), Avenida Brasil 4365, Manguinhos, Rio de Janeiro 210360-040, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patr\u00edcia B.","family":"da Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Celular, Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz (FIOCRUZ), Avenida Brasil 4365, Manguinhos, Rio de Janeiro 210360-040, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcos M.","family":"Batista","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Celular, Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz (FIOCRUZ), Avenida Brasil 4365, Manguinhos, Rio de Janeiro 210360-040, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fl\u00e1via da C.","family":"Camillo","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Tecnologia para Biodiversidade em Sa\u00fade\/LDFito, Instituto de Tecnologia em F\u00e1rmacos (Farmanguinhos), Funda\u00e7\u00e3o Oswaldo Cruz (FIOCRUZ), Avenida Brasil 4365, Manguinhos, Rio de Janeiro 210360-040, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9 M.","family":"Marques","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Tecnologia para Biodiversidade em Sa\u00fade\/LDFito, Instituto de Tecnologia em F\u00e1rmacos (Farmanguinhos), Funda\u00e7\u00e3o Oswaldo Cruz (FIOCRUZ), Avenida Brasil 4365, Manguinhos, Rio de Janeiro 210360-040, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lav\u00ednia","family":"de C. Brito","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Tecnologia para Biodiversidade em Sa\u00fade\/LDFito, Instituto de Tecnologia em F\u00e1rmacos (Farmanguinhos), Funda\u00e7\u00e3o Oswaldo Cruz (FIOCRUZ), Avenida Brasil 4365, Manguinhos, Rio de Janeiro 210360-040, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria R.","family":"Figueiredo","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Tecnologia para Biodiversidade em Sa\u00fade\/LDFito, Instituto de Tecnologia em F\u00e1rmacos (Farmanguinhos), Funda\u00e7\u00e3o Oswaldo Cruz (FIOCRUZ), Avenida Brasil 4365, Manguinhos, Rio de Janeiro 210360-040, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria de N. C.","family":"Soeiro","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Celular, Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz (FIOCRUZ), Avenida Brasil 4365, Manguinhos, Rio de Janeiro 210360-040, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,9,18]]},"reference":[{"key":"ref_1","unstructured":"WHO (2020, November 20). Chagas Disease (American Trypanosomiasis). Available online: https:\/\/www.who.int\/westernpacific\/health-topics\/chagas-disease."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"P\u00e9rez-Molina, J.A., Crespillo-And\u00fajar, C., Bosch-Nicolau, P., and Molina, I. (2020). Trypanocidal Treatment of Chagas Disease. Enferm. Infecc. Microbiol. 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