{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T15:21:57Z","timestamp":1780672917386,"version":"3.54.1"},"reference-count":36,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2021,9,23]],"date-time":"2021-09-23T00:00:00Z","timestamp":1632355200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"CONACYT-Mexico","award":["CB\/2006-67005"],"award-info":[{"award-number":["CB\/2006-67005"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Candida\u00a0albicans is the most commonly implicated agent in invasive human fungal infections. The disease could be presented as minimal symptomatic candidemia or can be fulminant sepsis. Candidemia is associated with a high rate of mortality and high healthcare and hospitalization costs. The surveillance programs have reported the distribution of other Candida species reflecting the trends and antifungal susceptibilities. Previous studies have demonstrated that C. glabrata more frequently presents fluconazole-resistant strains. Extracts from Mexican plants have been reported with activity against pulmonary mycosis, among them Colubrina greggii. In the present study, extracts from the aerial parts (leaves, flowers, and fruits) of this plant were evaluated against clinical isolates of several species of Candida (C. albicans, C. glabrata, C. parapsilosis, C. krusei, and C. tropicalis) by the broth microdilution assay. Through bioassay-guided fractionation, three antifungal glycosylated flavonoids were isolated and characterized. The isolated compounds showed antifungal activity only against C. glabrata resistant to fluconazole, and were non-toxic toward brine shrimp lethality bioassay and in vitro Vero cell line assay. The ethyl acetate and butanol extracts, as well as the fractions containing the mixture of flavonoids, were more active against Candida spp.<\/jats:p>","DOI":"10.3390\/molecules26195760","type":"journal-article","created":{"date-parts":[[2021,9,24]],"date-time":"2021-09-24T00:30:33Z","timestamp":1632443433000},"page":"5760","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Active Flavonoids from Colubrina greggii var. greggii S. Watson against Clinical Isolates of Candida spp."],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5434-2765","authenticated-orcid":false,"given":"Elda M.","family":"Melchor-Mart\u00ednez","sequence":"first","affiliation":[{"name":"Departamento de Qu\u00edmica Anal\u00edtica, Facultad de Medicina, Universidad Autonoma de Nuevo Leon, Av. Madero s\/n, Colonia Mitras Centro, Monterrey 64460, Nuevo Le\u00f3n, Mexico"},{"name":"School of Engineering and Sciences, Tecnologico de Monterrey, Monterrey 64849, Nuevo Le\u00f3n, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3128-0060","authenticated-orcid":false,"given":"Juan F.","family":"Tamez-Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica Anal\u00edtica, Facultad de Medicina, Universidad Autonoma de Nuevo Leon, Av. Madero s\/n, Colonia Mitras Centro, Monterrey 64460, Nuevo Le\u00f3n, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gloria Mar\u00eda","family":"Gonz\u00e1lez-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda, Facultad de Medicina, Universidad Autonoma de Nuevo Leon, Av. Madero s\/n, Colonia Mitras Centro, Monterrey 64460, Nuevo Le\u00f3n, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8177-3595","authenticated-orcid":false,"given":"David A.","family":"Silva-Mares","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica Anal\u00edtica, Facultad de Medicina, Universidad Autonoma de Nuevo Leon, Av. Madero s\/n, Colonia Mitras Centro, Monterrey 64460, Nuevo Le\u00f3n, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noem\u00ed","family":"Waksman-Minsky","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica Anal\u00edtica, Facultad de Medicina, Universidad Autonoma de Nuevo Leon, Av. Madero s\/n, Colonia Mitras Centro, Monterrey 64460, Nuevo Le\u00f3n, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis Alejandro","family":"P\u00e9rez-L\u00f3pez","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica Anal\u00edtica, Facultad de Medicina, Universidad Autonoma de Nuevo Leon, Av. Madero s\/n, Colonia Mitras Centro, Monterrey 64460, Nuevo Le\u00f3n, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ver\u00f3nica M.","family":"Rivas-Galindo","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica Anal\u00edtica, Facultad de Medicina, Universidad Autonoma de Nuevo Leon, Av. Madero s\/n, Colonia Mitras Centro, Monterrey 64460, Nuevo Le\u00f3n, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,9,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1016\/j.bmc.2013.12.010","article-title":"Synthesis and antifungal activity of substituted 2,4,6-pyrimidinetrione carbaldehyde hydrazones","volume":"22","author":"Neumann","year":"2014","journal-title":"Bioorg. Med. Chem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"110550","DOI":"10.1016\/j.biopha.2020.110550","article-title":"Immune defence to invasive fungal infections: A comprehensive review","volume":"130","author":"Pathakumari","year":"2020","journal-title":"Biomed. 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