{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T16:50:42Z","timestamp":1789750242310,"version":"4.0.1"},"reference-count":55,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2021,9,25]],"date-time":"2021-09-25T00:00:00Z","timestamp":1632528000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2019\/05856-7"],"award-info":[{"award-number":["2019\/05856-7"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["308127\/2018-80"],"award-info":[{"award-number":["308127\/2018-80"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The increasing incidence of antifungal resistance represents a great challenge in the medical area and, for this reason, new therapeutic alternatives for the treatment of fungal infections are urgently required. Cold atmospheric plasma (CAP) has been proposed as a promising alternative technique for the treatment of superficial candidiasis, with inhibitory effect both in vitro and in vivo. However, little is known on the association of CAP with conventional antifungals. The aim of this study was to evaluate the effects of the association between CAP and conventional polyene antifungals on Candida albicans biofilms. C. albicans SC 5314 and a clinical isolate were used to grow 24 or 48 h biofilms, under standardized conditions. After that, the biofilms were exposed to nystatin, amphotericin B and CAP, separately or in combination. Different concentrations of the antifungals and sequences of treatment were evaluated to establish the most effective protocol. Biofilms viability after the treatments was compared to negative control. Data were compared by One-way ANOVA and post hoc Tukey (5%). The results demonstrate that 5 min exposure to CAP showed more effective antifungal effect on biofilms when compared to nystatin and amphotericin B. Additionally, it was detected that CAP showed similar (but smaller in magnitude) effects when applied in association with nystatin and amphotericin B at 40 \u00b5g\/mL and 60 \u00b5g\/mL. Therefore, it can be concluded that the application of CAP alone was more effective against C. albicans biofilms than in combination with conventional polyene antifungal agents.<\/jats:p>","DOI":"10.3390\/molecules26195815","type":"journal-article","created":{"date-parts":[[2021,9,27]],"date-time":"2021-09-27T21:40:30Z","timestamp":1632778830000},"page":"5815","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Effect of Cold Atmospheric Plasma Jet Associated to Polyene Antifungals on Candida albicans Biofilms"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4961-6848","authenticated-orcid":false,"given":"Lady Daiane Pereira","family":"Leite","sequence":"first","affiliation":[{"name":"Science Applied to Oral Health, Graduate Program of Institute of Science and Technology, S\u00e3o Paulo State University (Unesp), S\u00e3o Jos\u00e9 dos Campos 12245-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7060-7604","authenticated-orcid":false,"given":"Maria Alcion\u00e9ia Carvalho de","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Oral Biopathology, Graduate Program of Institute of Science and Technology, S\u00e3o Paulo State University (Unesp), S\u00e3o Jos\u00e9 dos Campos 12245-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1819-3598","authenticated-orcid":false,"given":"Mariana Raquel da Cruz","family":"Vegian","sequence":"additional","affiliation":[{"name":"Science Applied to Oral Health, Graduate Program of Institute of Science and Technology, S\u00e3o Paulo State University (Unesp), S\u00e3o Jos\u00e9 dos Campos 12245-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9453-3905","authenticated-orcid":false,"given":"Aline da Gra\u00e7a","family":"Sampaio","sequence":"additional","affiliation":[{"name":"Science Applied to Oral Health, Graduate Program of Institute of Science and Technology, S\u00e3o Paulo State University (Unesp), S\u00e3o Jos\u00e9 dos Campos 12245-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2844-3156","authenticated-orcid":false,"given":"Thalita Mayumi Castaldelli","family":"Nishime","sequence":"additional","affiliation":[{"name":"Leibniz Institute for Plasma Science and Technology, 17489 Greifswald, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9821-8088","authenticated-orcid":false,"given":"Konstantin Georgiev","family":"Kostov","sequence":"additional","affiliation":[{"name":"Department of Physics, Guaratinguet\u00e1 Faculty of Engineering, S\u00e3o Paulo State University (Unesp), Guaratinguet\u00e1 12516-410, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2416-2173","authenticated-orcid":false,"given":"Cristiane Yumi","family":"Koga-Ito","sequence":"additional","affiliation":[{"name":"Science Applied to Oral Health, Graduate Program of Institute of Science and Technology, S\u00e3o Paulo State University (Unesp), S\u00e3o Jos\u00e9 dos Campos 12245-000, Brazil"},{"name":"Oral Biopathology, Graduate Program of Institute of Science and Technology, S\u00e3o Paulo State University (Unesp), S\u00e3o Jos\u00e9 dos Campos 12245-000, Brazil"},{"name":"Department of Environment Engineering, Institute of Science and Technology, S\u00e3o Paulo State University (Unesp), S\u00e3o Jos\u00e9 dos Campos 12247-016, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,9,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Mount, H.O., Revie, N.M., Todd, R.T., Anstett, K., Collins, C., Costanzo, M., Boone, C., Robbins, N., Selmecki, A., and Cowen, L.E. (2018). Global analysis of genetic circuitry and adaptive mechanisms enabling resistance to the azole antifungal drugs. PLoS Genet., 14.","DOI":"10.1371\/journal.pgen.1007319"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1783","DOI":"10.1158\/0008-5472.CAN-16-2929","article-title":"Primer and perspective for non-microbiologists","volume":"77","author":"Thomas","year":"2017","journal-title":"Cancer Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"22434","DOI":"10.3402\/jom.v5i0.22434","article-title":"Interactions of Candida albicans with host epithelial surfaces","volume":"5","author":"Williams","year":"2013","journal-title":"J. Oral Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1086\/596757","article-title":"Clinical practice guidelines for the management of candidiasis: 2009 Update by the Infectious Diseases Society of America","volume":"48","author":"Pappas","year":"2009","journal-title":"Clin. Infect. Dis."},{"key":"ref_5","first-page":"263","article-title":"Oral candidiasis: Pathogenesis, clinical presentation, diagnosis and treatment strategies","volume":"41","author":"Lalla","year":"2013","journal-title":"J. Calif. Dent. Assoc."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1391","DOI":"10.3389\/fmicb.2015.01391","article-title":"Clinical appearance of oral Candida infection and therapeutic strategies","volume":"6","author":"Patil","year":"2015","journal-title":"Front. Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Ferreira, J.N.A.R., Fricton, J., and Rhodus, N. (2017). Orofacial disorders. Current Therapies in Orofacial Pain and Oral Medicine, Springer.","DOI":"10.1007\/978-3-319-51508-3"},{"key":"ref_8","first-page":"e172","article-title":"Therapeutic tools for oral candidiasis: Current and new antifungal drugs","volume":"24","author":"Sevillano","year":"2019","journal-title":"Med. Oral Patol. Oral Cir. Bucal"},{"key":"ref_9","first-page":"e576","article-title":"Current treatment of oral candidiasis: A literature review","volume":"6","year":"2014","journal-title":"J. Clin. Exp. Dent."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"3","DOI":"10.2174\/187152107779314142","article-title":"Unique applications of novel antifungal drug combinations","volume":"6","author":"Onyewu","year":"2007","journal-title":"Antiinfect. Agents Med. Chem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1007\/s10096-013-2009-3","article-title":"Candida glabrata: A review of its features and resistance","volume":"33","author":"Rodrigues","year":"2014","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1165","DOI":"10.1097\/00006454-200212000-00017","article-title":"Comparison of fluconazole and nystatin oral suspensions for treatment of oral candidiasis in infants","volume":"21","author":"Goins","year":"2002","journal-title":"Pediatr. Infect. Dis. J."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1063\/1.2940325","article-title":"Cold atmospheric pressure air plasma jet for medical applications","volume":"92","author":"Kolb","year":"2008","journal-title":"Appl. Phys. Lett."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1592\/phco.28.5.614","article-title":"Current options in antifungal pharmacotherapy","volume":"28","author":"Mohr","year":"2008","journal-title":"Pharmacotherapy"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.pdpdt.2015.12.007","article-title":"Cold atmospheric plasma inhibits the growth of Candida albicans by affecting ergosterol biosynthesis and suppresses the fungal virulence factors in vitro","volume":"13","author":"Shapoorzadeh","year":"2016","journal-title":"Photodiagn. Photodyn. Ther."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"e383","DOI":"10.1016\/S1473-3099(17)30316-X","article-title":"The global problem of antifungal resistance: Prevalence, mechanisms, and management","volume":"17","author":"Perlin","year":"2017","journal-title":"Lancet Infect. Dis."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Butts, A., and Krysan, D.J. (2012). Antifungal Drug Discovery: Something Old and Something New. PLoS Pathog., 8.","DOI":"10.1371\/journal.ppat.1002870"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"115019","DOI":"10.1088\/1367-2630\/11\/11\/115019","article-title":"Nosocomial infections\u2013a new approach towards preventive medicine using plasmas","volume":"11","author":"Morfill","year":"2009","journal-title":"New J. Phys."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"4242","DOI":"10.1128\/AEM.07235-11","article-title":"Contact-free inactivation of Candida albicans biofilms by cold atmospheric air plasma","volume":"78","author":"Maisch","year":"2012","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"384","DOI":"10.4012\/dmj.2010-190","article-title":"Microbicidal activities of low frequency atmospheric pressure plasma jets on oral pathogens","volume":"30","author":"Yamazaki","year":"2011","journal-title":"Dent. Mater. J."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.cpme.2017.06.002","article-title":"Cold atmospheric pressure plasma jet modulates Candida albicans virulence traits","volume":"7\u20138","author":"Borges","year":"2017","journal-title":"Clin. Plasma Med."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1099\/jmm.0.013714-0","article-title":"Killing of adherent oral microbes by a non-thermal atmospheric plasma jet","volume":"59","author":"Rupf","year":"2010","journal-title":"J. Med. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"021501","DOI":"10.1063\/1.3530434","article-title":"Atmospheric pressure cold plasma as an antifungal therapy","volume":"98","author":"Sun","year":"2011","journal-title":"Appl. Phys. Lett."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Borges, A.C., Lima, G.D.M.G., Nishime, T.M.C., Gontijo, A.V.L., Kostov, K.G., and Koga-Ito, C.Y. (2018). Amplitude-modulated cold atmospheric pressure plasma jet for treatment of oral candidiasis: In vivo study. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0199832"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"870","DOI":"10.2174\/0929867321666131218094848","article-title":"Synergy against fungal pathogens: Working together is better than working alone","volume":"21","author":"Musiol","year":"2014","journal-title":"Curr. Med. Chem."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Sun, Y., Yu, S., Sun, P., Wu, H., Zhu, W., Liu, W., Zhang, J., Fang, J., and Li, R. (2012). Inactivation of candida biofilms by non-thermal plasma and its enhancement for fungistatic effect of antifungal drugs. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0040629"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"108772","DOI":"10.1016\/j.abb.2021.108772","article-title":"Inhibitory effects of cold atmospheric plasma on the growth, virulence factors and HSP90 gene expression in Candida albicans","volume":"700","author":"Noormohammadi","year":"2021","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"111","DOI":"10.14295\/bds.2018.v21i1.1489","article-title":"Effect of ozonized olive oil on oral levels of Candida spp. in patients with denture stomatitis","volume":"21","author":"Crastechini","year":"2018","journal-title":"Braz. Dent. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1016\/j.bjid.2018.10.278","article-title":"Time of catheter removal in candidemia and mortality","volume":"22","author":"Nucci","year":"2018","journal-title":"Braz. J. Infect. Dis."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1717","DOI":"10.1007\/s10096-018-3304-9","article-title":"Clinical characteristics and predictors of mortality in patients with candidemia: A six-year retrospective study","volume":"37","author":"Jia","year":"2018","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Silva, S., Rodrigues, C.F., Ara\u00fajo, D., Rodrigues, M.E., and Henriques, M. (2017). Candida species biofilms\u2019 antifungal resistance. J. Fungi, 3.","DOI":"10.3390\/jof3010008"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"ftw018","DOI":"10.1093\/femspd\/ftw018","article-title":"Pathogenesis of Candida albicans biofilm","volume":"74","author":"Tsui","year":"2016","journal-title":"Pathog. Dis."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Tumbarello, M., Fiori, B., Trecarichi, E.M., Posteraro, P., Losito, A.R., De Luca, A., Sanguinetti, M., Fadda, G., Cauda, R., and Posteraro, B. (2012). Risk factors and outcomes of candidemia caused by biofilm-forming isolates in a tertiary care hospital. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0033705"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Pawlat, J., Kwiatkowski, M., Terebun, P., Chudzik, B., and Gagos, M. (2017, January 3\u20136). Candida albicans inactivation with DBD He\/O2 plasma jet. Proceedings of the 2017 International Conference on Electromagnetic Devices and Processes in Environment Protection with Seminar Applications Superconductors ELMECO and AoS, Naleczow, Poland.","DOI":"10.1109\/ELMECO.2017.8267745"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"e01163-18","DOI":"10.1128\/AEM.01163-18","article-title":"Nonthermal plasma jet treatment negatively affects the viability and structure of Candida albicans SC5314 biofilms","volume":"84","author":"Handorf","year":"2018","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1111\/j.1574-695X.2012.00942.x","article-title":"Application of atmospheric pressure nonthermal plasma for the in vitro eradication of bacterial biofilms","volume":"65","author":"Alkawareek","year":"2012","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1016\/j.ijantimicag.2014.01.025","article-title":"Atmospheric pressure plasmas: Infection control and bacterial responses","volume":"43","author":"Murphy","year":"2014","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Aparecida Delben, J., Evelin Zago, C., Tyhovych, N., Duarte, S., and Eduardo Vergani, C. (2016). Effect of atmospheric-pressure cold plasma on pathogenic oral biofilms and in vitro reconstituted oral epithelium. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0155427"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"462","DOI":"10.12968\/jowc.2017.26.8.462","article-title":"Cold atmospheric pressure plasmas exhibit antimicrobial properties against critical bacteria and yeast species","volume":"26","author":"Wiegand","year":"2017","journal-title":"J. Wound Care"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"6891","DOI":"10.1021\/acsomega.8b03640","article-title":"Comparative study of Candida albicans inactivation by nonthermal plasma on stainless steel with and without diamond-like carbon film","volume":"4","author":"Sene","year":"2019","journal-title":"ACS Omega"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.cmi.2015.09.018","article-title":"Biofilm formation is a risk factor for mortality in patients with Candida albicans bloodstream infection\u2014Scotland, 2012\u20132013","volume":"22","author":"Rajendran","year":"2016","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1128\/microbiolspec.FUNK-0002-2016","article-title":"Antifungal Drugs: The Current Armamentarium and Development of New Agents","volume":"4","author":"Robbins","year":"2016","journal-title":"Microbiol. Spectr."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1016\/j.diagmicrobio.2016.08.002","article-title":"Synthesis, antifungal activity of caffeic acid derivative esters, and their synergism with fluconazole and nystatin against Candida spp","volume":"86","author":"Sardi","year":"2016","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"5603","DOI":"10.1128\/AAC.00612-12","article-title":"Toxicity mechanisms of amphotericin B and its neutralization by conjugation with arabinogalactan","volume":"56","author":"Kagan","year":"2012","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1016\/j.tibtech.2017.07.012","article-title":"Tracking the Penetration of Plasma Reactive Species in Tissue Models","volume":"36","author":"Szili","year":"2018","journal-title":"Trends Biotechnol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"024001","DOI":"10.1088\/2058-6272\/19\/2\/024001","article-title":"A novel approach to regulate cell membrane permeability for ATP and NADH formation in Saccharomyces cerevisiae induced by air cold plasma","volume":"19","author":"Dong","year":"2017","journal-title":"Plasma Sci. Technol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1111\/jam.13780","article-title":"Antibacterial efficacy and mechanisms of action of low power atmospheric pressure cold plasma: Membrane permeability, biofilm penetration and antimicrobial sensitization","volume":"125","author":"Brun","year":"2018","journal-title":"J. Appl. Microbiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1038\/sj.bdj.2017.886","article-title":"Diagnosis and management of oral candidosis","volume":"223","author":"Lewis","year":"2017","journal-title":"Br. Dent. J."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"6627","DOI":"10.1128\/AAC.03570-14","article-title":"The production of reactive oxygen species is a universal action mechanism of Amphotericin B against pathogenic yeasts and contributes to the fungicidal effect of this drug","volume":"58","author":"Casas","year":"2014","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1111\/jam.13429","article-title":"Microbiological interactions with cold plasma","volume":"123","author":"Bourke","year":"2017","journal-title":"J. Appl. Microbiol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1093\/mmy\/myx143","article-title":"Oxidative and nitrosative stress responses during macrophage Candida albicans biofilm interaction","volume":"57","author":"Baronetti","year":"2019","journal-title":"Med. Mycol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1080\/08927014.2016.1212021","article-title":"Impact of oxidative and osmotic stresses on Candida albicans biofilm formation","volume":"32","author":"Pemmaraju","year":"2016","journal-title":"Biofouling"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Komalapriya, C., Kaloriti, D., Tillmann, A.T., Yin, Z., Herrero-de-Dios, C., Jacobsen, M.D., Belmonte, R.C., Cameron, G., Haynes, K., and Grebogi, C. (2015). Integrative model of oxidative stress adaptation in the fungal pathogen Candida albicans. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0137750"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1128\/AAC.46.1.245-247.2002","article-title":"In vitro synergy of caspofungin and amphotericin B against Aspergillus and Fusarium spp","volume":"46","author":"Arikan","year":"2002","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_55","unstructured":"(2008). CLSI M38-A2 Reference Method for Broth Dilution Antifungal Susceptibility Testing of Filamentous Fungi; Approved Standard\u2014Second Edition. Clin. Lab. Stand. Inst., 28, 29."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/26\/19\/5815\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:04:59Z","timestamp":1760166299000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/26\/19\/5815"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,25]]},"references-count":55,"journal-issue":{"issue":"19","published-online":{"date-parts":[[2021,10]]}},"alternative-id":["molecules26195815"],"URL":"https:\/\/doi.org\/10.3390\/molecules26195815","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,9,25]]}}}