{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T04:46:28Z","timestamp":1770957988714,"version":"3.50.1"},"reference-count":41,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2021,10,4]],"date-time":"2021-10-04T00:00:00Z","timestamp":1633305600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["MOST-109-2314-B-038-122"],"award-info":[{"award-number":["MOST-109-2314-B-038-122"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004700","name":"Taipei Medical University","doi-asserted-by":"publisher","award":["TMU-DP2-110-21121-03-C-06-02"],"award-info":[{"award-number":["TMU-DP2-110-21121-03-C-06-02"]}],"id":[{"id":"10.13039\/501100004700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Oral cancers, hepatocellular carcinoma, and colorectal cancers are the three most common cancers, leading to 18,000 cases of cancer-related mortality in Taiwan per year. To bridge the gap towards clinical translation, we developed a circulating tumor cell (CTC) organoid culture workflow that efficiently expands CTC from patients to test Antrodia Cinnamomea mycelium-derived bioactive compounds. Three ACM-derived bioactive compounds were evaluated for tumor chemosensitization characteristics. Significant and consistent cytotoxic\/5-FU sensitizing effects of GKB202 were found on 8 different patient-derived tumors. Acute toxicity profile and hepatic metabolism of GKB202 in rats suggest GKB202 is rapidly cleared by liver and is well tolerated up to the dose of 20 mg\/kg. This comprehensive study provides new evidence that liquid fermentation of Antrodia cinnamomea mycelium (ACM) contains bioactive compounds that lead to effective control of CTC, especially when combined with 5-FU. Together, these data suggest ACM-derived GKB202 may be considered for further clinical investigation in the context of 5-FU-based combination therapy.<\/jats:p>","DOI":"10.3390\/molecules26196018","type":"journal-article","created":{"date-parts":[[2021,10,8]],"date-time":"2021-10-08T21:23:55Z","timestamp":1633728235000},"page":"6018","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Patient-Derived Tumor Chemosensitization of GKB202, an Antrodia Cinnamomea Mycelium-Derived Bioactive Compound"],"prefix":"10.3390","volume":"26","author":[{"given":"Tsung-Ju","family":"Li","sequence":"first","affiliation":[{"name":"Biotech Research Institute, Grape King Bio, Taoyuan 32542, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ting-Wei","family":"Lin","sequence":"additional","affiliation":[{"name":"Biotech Research Institute, Grape King Bio, Taoyuan 32542, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shih-Pei","family":"Wu","sequence":"additional","affiliation":[{"name":"CancerFree Biotech Ltd., Taipei City 106, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hsin-Tung","family":"Chu","sequence":"additional","affiliation":[{"name":"Biotech Research Institute, Grape King Bio, Taoyuan 32542, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu-Hsuan","family":"Kuo","sequence":"additional","affiliation":[{"name":"Biotech Research Institute, Grape King Bio, Taoyuan 32542, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeng-Fong","family":"Chiou","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, Taipei Medical University Hospital, Taipei City 110, Taiwan"},{"name":"TMU Research Center of Cancer Translational Medicine, Taipei Medical University, Taipei City 110, Taiwan"},{"name":"Department of Radiology, School of Medicine, College of Medicine, Taipei Medical University, Taipei City 110, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4247-1981","authenticated-orcid":false,"given":"Long-Sheng","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, Taipei Medical University Hospital, Taipei City 110, Taiwan"},{"name":"Graduate Institute of Biomedical Materials and Tissue Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei City 110, Taiwan"},{"name":"International Ph.D. Program in Biomedical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei City 110, Taiwan"},{"name":"International Ph.D. Program for Cell Therapy and Regenerative Medicine, College of Medicine, Taipei Medical University, Taipei City 11031, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chin-Chu","family":"Chen","sequence":"additional","affiliation":[{"name":"Biotech Research Institute, Grape King Bio, Taoyuan 32542, Taiwan"},{"name":"Department of Food Science, Nutrition, and Nutraceutical Biotechnology, Shih Chien University, Taipei City 104, Taiwan"},{"name":"Institute of Food Science and Technology, National Taiwan University, Taipei City 10617, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,10,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.pharmthera.2013.04.001","article-title":"Recent Research and Development of Antrodia Cinnamomea","volume":"139","author":"Lu","year":"2013","journal-title":"Pharmacol. 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