{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T08:47:40Z","timestamp":1790758060656,"version":"4.1.0"},"reference-count":46,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2021,10,21]],"date-time":"2021-10-21T00:00:00Z","timestamp":1634774400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"R\u00e9gion R\u00e9union, FEDER RE00018978 European Funds (EU\u2014R\u00e9gion R\u00e9union\u2014French State national counterpart),","award":["RE00018978"],"award-info":[{"award-number":["RE00018978"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Introduction: The pleiotropic protective effects of high-density lipoproteins (HDLs) on cerebral ischemia have never been tested under acute hyperglycemic conditions. The aim of this study is to evaluate the potential neuroprotective effect of HDL intracarotid injection in a mouse model of middle cerebral artery occlusion (MCAO) under hyperglycemic conditions. Methods: Forty-two mice were randomized to receive either an intracarotid injection of HDLs or saline. Acute hyperglycemia was induced by an intraperitoneal injection of glucose (2.2 g\/kg) 20 min before MCAO. Infarct size (2,3,5-triphenyltetrazolium chloride (TTC)-staining), blood\u2013brain barrier leakage (IgG infiltration), and hemorrhagic changes (hemoglobin assay by ELISA and hemorrhagic transformation score) were analyzed 24 h post-stroke. Brain tissue inflammation (IL-6 by ELISA, neutrophil infiltration and myeloperoxidase by immunohisto-fluorescence) and apoptosis (caspase 3 activation) were also assessed. Results: Intraperitoneal D-glucose injection allowed HDL- and saline-treated groups to reach a blood glucose level of 300 mg\/dl in the acute phase of cerebral ischemia. HDL injection did not significantly reduce mortality (19% versus 29% in the saline-injected group) or cerebral infarct size (p = 0.25). Hemorrhagic transformations and inflammation parameters were not different between the two groups. In addition, HDL did not inhibit apoptosis under acute hyperglycemic conditions. Conclusion: We observed a nonsignificant decrease in cerebral infarct size in the HDL group. The deleterious consequences of reperfusion such as hemorrhagic transformation or inflammation were not improved by HDL infusion. In acute hyperglycemia, HDLs are not potent enough to counteract the adverse effects of hyperglycemia. The addition of antioxidants to therapeutic HDLs could improve their neuroprotective capacity.<\/jats:p>","DOI":"10.3390\/molecules26216365","type":"journal-article","created":{"date-parts":[[2021,10,21]],"date-time":"2021-10-21T23:27:39Z","timestamp":1634858859000},"page":"6365","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Lack of Neuroprotective Effects of High-Density Lipoprotein Therapy in Stroke under Acute Hyperglycemic Conditions"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0305-8681","authenticated-orcid":false,"given":"David","family":"Couret","sequence":"first","affiliation":[{"name":"UMR 1188 Diab\u00e8te Ath\u00e9rothrombose Th\u00e9rapies R\u00e9union Oc\u00e9an Indien (D\u00e9TROI), Universit\u00e9 de la R\u00e9union, Inserm, Plateforme CYROI, F-97490 Sainte-Clotilde, France"},{"name":"Service de Neuror\u00e9animation, Centre Hospitalo-Universitaire de La R\u00e9union, 97410 Saint-Pierre de La R\u00e9union, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cynthia","family":"Planesse","sequence":"additional","affiliation":[{"name":"UMR 1188 Diab\u00e8te Ath\u00e9rothrombose Th\u00e9rapies R\u00e9union Oc\u00e9an Indien (D\u00e9TROI), Universit\u00e9 de la R\u00e9union, Inserm, Plateforme CYROI, F-97490 Sainte-Clotilde, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica","family":"Patche","sequence":"additional","affiliation":[{"name":"UMR 1188 Diab\u00e8te Ath\u00e9rothrombose Th\u00e9rapies R\u00e9union Oc\u00e9an Indien (D\u00e9TROI), Universit\u00e9 de la R\u00e9union, Inserm, Plateforme CYROI, F-97490 Sainte-Clotilde, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2032-518X","authenticated-orcid":false,"given":"Nicolas","family":"Diotel","sequence":"additional","affiliation":[{"name":"UMR 1188 Diab\u00e8te Ath\u00e9rothrombose Th\u00e9rapies R\u00e9union Oc\u00e9an Indien (D\u00e9TROI), Universit\u00e9 de la R\u00e9union, Inserm, Plateforme CYROI, F-97490 Sainte-Clotilde, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3719-7903","authenticated-orcid":false,"given":"Brice","family":"Nativel","sequence":"additional","affiliation":[{"name":"UMR 1188 Diab\u00e8te Ath\u00e9rothrombose Th\u00e9rapies R\u00e9union Oc\u00e9an Indien (D\u00e9TROI), Universit\u00e9 de la R\u00e9union, Inserm, Plateforme CYROI, F-97490 Sainte-Clotilde, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steeve","family":"Bourane","sequence":"additional","affiliation":[{"name":"UMR 1188 Diab\u00e8te Ath\u00e9rothrombose Th\u00e9rapies R\u00e9union Oc\u00e9an Indien (D\u00e9TROI), Universit\u00e9 de la R\u00e9union, Inserm, Plateforme CYROI, F-97490 Sainte-Clotilde, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Meilhac","sequence":"additional","affiliation":[{"name":"UMR 1188 Diab\u00e8te Ath\u00e9rothrombose Th\u00e9rapies R\u00e9union Oc\u00e9an Indien (D\u00e9TROI), Universit\u00e9 de la R\u00e9union, Inserm, Plateforme CYROI, F-97490 Sainte-Clotilde, France"},{"name":"CIC-EC 1410, Centre Hospitalo-Universitaire de La R\u00e9union, 97410 Saint-Pierre de La R\u00e9union, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,10,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1146\/annurev.med.56.082103.104751","article-title":"Metabolic syndrome: A clinical and molecular perspective","volume":"56","author":"Moller","year":"2005","journal-title":"Annu. 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