{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:45:07Z","timestamp":1785437107404,"version":"3.56.0"},"reference-count":33,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2021,10,25]],"date-time":"2021-10-25T00:00:00Z","timestamp":1635120000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001655","name":"German Academic Exchange Service","doi-asserted-by":"publisher","award":["00 DAAR Rise Program"],"award-info":[{"award-number":["00 DAAR Rise Program"]}],"id":[{"id":"10.13039\/501100001655","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Studies on polyphenols and flavonoids in natural products reveal benefits in the prevention of multiple diseases. Proper extraction, treatment of extracts, and quantification of polyphenols and flavonoids demand attention from the scientific community in order to report more specific biological action. Total polyphenol content (TPC) and total flavonoid content (TFC) (measured at three different times) of ethanol, methanol and acetone extracts of Mauritia flexuosa (aguaje) and Theobroma grandiflorum (copoaz\u00fa) fresh pulp, from the Colombian Amazon region, were evaluated with the purpose of focusing in the polyphenol\/flavonoid proportion and its effective antioxidant activity. This objective could help to explain specific flavonoid biological action based on higher flavonoid proportion rather than higher total polyphenol content. Differences in extracting solvents resulted in statistically significant different yields; the highest TPC was observed with acetone 70% in Mauritia flexuosa and ethanol 80% for T. grandiflorum. The best flavonoid\/polyphenol ratio in M. flexuosa was about 1:2.4 and 1:12.8 in T. grandiflorum and the antioxidant efficacy was proportionally higher for flavonoids extracted from T. grandiflorum. HPLC analysis revealed 54 \u00b5g\/g of the flavonoid kaempferol in M. Flexuosa and 29 \u00b5g\/g in T. grandiflorum. Further studies evaluating this proportionality, in seeds or peel of fruits, as well as, other specific biological activities, could help to understand the detailed flavonoid action without focusing on the high total polyphenol content.<\/jats:p>","DOI":"10.3390\/molecules26216431","type":"journal-article","created":{"date-parts":[[2021,10,25]],"date-time":"2021-10-25T21:42:05Z","timestamp":1635198125000},"page":"6431","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Flavonoid\/Polyphenol Ratio in Mauritia flexuosa and Theobroma grandiflorum as an Indicator of Effective Antioxidant Action"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9944-4573","authenticated-orcid":false,"given":"Juan Carlos","family":"Carmona-Hernandez","sequence":"first","affiliation":[{"name":"Grupo de Investigaci\u00f3n M\u00e9dica, L\u00ednea Metabolismo-Nutrici\u00f3n-Polifenoles (MeNutrO), Universidad de Manizales, Manizales 17000, Colombia"},{"name":"Research Group on Nutrition, Metabolism and Food Security, Universidad de Caldas, Manizales 17000, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mai","family":"Le","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy and Molecular Biotechnology, Faculty of Biosciences, Heidelberg University, 69120 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Mar\u00eda","family":"Id\u00e1rraga-Mej\u00eda","sequence":"additional","affiliation":[{"name":"Research Group on Nutrition, Metabolism and Food Security, Universidad de Caldas, Manizales 17000, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5621-2166","authenticated-orcid":false,"given":"Clara Helena","family":"Gonz\u00e1lez-Correa","sequence":"additional","affiliation":[{"name":"Research Group on Nutrition, Metabolism and Food Security, Universidad de Caldas, Manizales 17000, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,10,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"980","DOI":"10.1016\/j.foodchem.2010.03.017","article-title":"Chemical composition, anthocyanins, non-anthocyanin phenolics and antioxidant activity of wild bilberry (Vaccinium meridionale Swartz) from Colombia","volume":"122","author":"Riedl","year":"2010","journal-title":"Food Chem."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Solverson, P.M., Rumpler, W.V., Leger, J.L., Redan, B.W., Ferruzzi, M.G., Baer, D.J., Castonguay, T.W., and Novotny, J.A. 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