{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T23:58:29Z","timestamp":1792540709327,"version":"4.5.0"},"reference-count":36,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2021,11,10]],"date-time":"2021-11-10T00:00:00Z","timestamp":1636502400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>A mild and versatile method based on Cu-catalyzed [2+3] cycloaddition (Huisgen-Meldal-Sharpless reaction) was developed to tether 3,3\u2019-((4-(prop-2-yn-1-yloxy)phenyl)methylene)bis(4-hydroxyquinolin-2(1H)-ones) with 4-azido-2-quinolones in good yields. This methodology allowed attaching three quinolone molecules via a triazole linker with the proposed mechanism. The products are interesting precursors for their anti-proliferative activity. Compound 8g was the most active one, achieving IC50 = 1.2 \u00b1 0.2 \u00b5M and 1.4 \u00b1 0.2 \u00b5M against MCF-7 and Panc-1 cell lines, respectively. Moreover, cell cycle analysis of cells MCF-7 treated with 8g showed cell cycle arrest at the G2\/M phase (supported by Caspase-3,8,9, Cytochrome C, BAX, and Bcl-2 studies). Additionally, significant pro-apoptotic activity is indicated by annexin V-FITC staining.<\/jats:p>","DOI":"10.3390\/molecules26226798","type":"journal-article","created":{"date-parts":[[2021,11,11]],"date-time":"2021-11-11T23:04:46Z","timestamp":1636671886000},"page":"6798","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Design and Synthesis of (2-oxo-1,2-Dihydroquinolin-4-yl)-1,2,3-triazole Derivatives via Click Reaction: Potential Apoptotic Antiproliferative Agents"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3804-9729","authenticated-orcid":false,"given":"Essmat M.","family":"El-Sheref","sequence":"first","affiliation":[{"name":"Chemistry Department, Faculty of Science, Minia University, El-Minia 61519, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3460-0667","authenticated-orcid":false,"given":"Mohammed A. I.","family":"Elbastawesy","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Organic Chemistry, Faculty of Pharmacy, Al-Azhar University, Assiut 71524, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan B.","family":"Brown","sequence":"additional","affiliation":[{"name":"Program in Chemistry, Florida Institute of Technology, 150 W University Blvd, Melbourne, FL 32901, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3035-661X","authenticated-orcid":false,"given":"Ahmed M.","family":"Shawky","sequence":"additional","affiliation":[{"name":"Science and Technology Unit (STU), Umm Al-Qura University, Makkah 21955, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7510-8428","authenticated-orcid":false,"given":"Hesham A. M.","family":"Gomaa","sequence":"additional","affiliation":[{"name":"Pharmacology Department, College of Pharmacy, Jouf University, Sakaka 72314, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4845-3191","authenticated-orcid":false,"given":"Stefan","family":"Br\u00e4se","sequence":"additional","affiliation":[{"name":"Institute of Organic Chemistry, Karlsruhe Institute of Technology, 76131 Karlsruhe, Germany"},{"name":"Institute of Biological and Chemical Systems (IBCS-FMS), Karlsruhe Institute of Technology, 76344 Eggenstein-Leopoldshafen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6834-6548","authenticated-orcid":false,"given":"Bahaa G. M.","family":"Youssif","sequence":"additional","affiliation":[{"name":"Pharmaceutical Organic Chemistry Department, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,11,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4064","DOI":"10.1021\/acssuschemeng.6b01010","article-title":"Synthesis of quinolines: A green perspective","volume":"4","author":"Batista","year":"2016","journal-title":"ACS Sustain. Chem. Eng."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"103291","DOI":"10.1016\/j.bioorg.2019.103291","article-title":"Recent advances in the synthetic and medicinal perspective of quinolones. A review","volume":"92","author":"Dhiman","year":"2019","journal-title":"Bioorg. 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