{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T05:33:50Z","timestamp":1773034430430,"version":"3.50.1"},"reference-count":55,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2021,11,18]],"date-time":"2021-11-18T00:00:00Z","timestamp":1637193600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Ferritin, a spherically shaped protein complex, is responsible for iron storage in bacteria, plants, animals, and humans. Various ferritin iron core compositions in organisms are associated with specific living requirements, health state, and different biochemical roles of ferritin isomers. Magnetoferritin, a synthetic ferritin derivative, serves as an artificial model system of unusual iron phase structures found in humans. We present the results of a complex structural study of magnetoferritins prepared by controlled in vitro synthesis. Using various complementary methods, it was observed that manipulation of the synthesis technology can improve the physicochemical parameters of the system, which is useful in applications. Thus, a higher synthesis temperature leads to an increase in magnetization due to the formation of the magnetite phase. An increase in the iron loading factor has a more pronounced impact on the protein shell structure in comparison with the pH of the aqueous medium. On the other hand, a higher loading factor at physiological temperature enhances the formation of an amorphous phase instead of magnetite crystallization. It was confirmed that the iron-overloading effect alone (observed during pathological events) cannot contribute to the formation of magnetite.<\/jats:p>","DOI":"10.3390\/molecules26226960","type":"journal-article","created":{"date-parts":[[2021,11,19]],"date-time":"2021-11-19T02:43:09Z","timestamp":1637289789000},"page":"6960","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The Impact of Redox, Hydrolysis and Dehydration Chemistry on the Structural and Magnetic Properties of Magnetoferritin Prepared in Variable Thermal Conditions"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2284-3482","authenticated-orcid":false,"given":"Lucia","family":"Balej\u010d\u00edkov\u00e1","sequence":"first","affiliation":[{"name":"Institute of Hydrology SAS, D\u00fabravsk\u00e1 Cesta 9, 84104 Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8133-5352","authenticated-orcid":false,"given":"Karel","family":"Saksl","sequence":"additional","affiliation":[{"name":"Institute of Materials Research Slovak Academy of Sciences, Watsonova 47, 04001 Ko\u0161ice, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7793-9564","authenticated-orcid":false,"given":"Jozef","family":"Kov\u00e1\u010d","sequence":"additional","affiliation":[{"name":"Institute of Experimental Physic SAS, Watsonova 47, 04001 Ko\u0161ice, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1232-8519","authenticated-orcid":false,"given":"Anne","family":"Martel","sequence":"additional","affiliation":[{"name":"Institut Laue-Langevin, 71 Avenue des Martyrs, 38042 Grenoble, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9315-4188","authenticated-orcid":false,"given":"Vasil M.","family":"Garamus","sequence":"additional","affiliation":[{"name":"Helmholtz-Zentrum Hereon, Max-Planck-Street 1, 21502 Geesthacht, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0721-4653","authenticated-orcid":false,"given":"Mikhail V.","family":"Avdeev","sequence":"additional","affiliation":[{"name":"Joint Institute for Nuclear Research, Joliot-Curie Str. 6, 141980 Dubna, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6532-8902","authenticated-orcid":false,"given":"Viktor I.","family":"Petrenko","sequence":"additional","affiliation":[{"name":"BCMaterials, Basque Centre for Materials, Applications and Nanostructures, 48940 Leioa, Spain"},{"name":"IKERBASQUE, Basque Foundation for Science, 48009 Bilbao, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5750-5788","authenticated-orcid":false,"given":"L\u00e1szl\u00f3","family":"Alm\u00e1sy","sequence":"additional","affiliation":[{"name":"Institute for Energy Security and Environmental Safety, Centre for Energy Research, Konkoly Thege Mi-klos ut 29-33, 1121 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5278-9504","authenticated-orcid":false,"given":"Peter","family":"Kop\u010dansk\u00fd","sequence":"additional","affiliation":[{"name":"Institute of Experimental Physic SAS, Watsonova 47, 04001 Ko\u0161ice, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,11,18]]},"reference":[{"key":"ref_1","first-page":"1575","article-title":"Sur la cristallisation de la ferritine","volume":"19","author":"Laufberger","year":"1937","journal-title":"Bull. Soc. Chim. Biol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Palombarini, F., Di Fabio, E., Boffi, A., Macone, A., and Bonamore, A. (2020). Ferritin Nanocages for Protein Delivery to Tumor Cells. Molecules, 25.","DOI":"10.3390\/molecules25040825"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"17080","DOI":"10.1021\/acsnano.0c06670","article-title":"His-mediated reversible self-assembly of ferritin nanocages through two different switches for encapsulation of cargo molecules","volume":"14","author":"Gu","year":"2020","journal-title":"ACS Nano"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1016\/j.biomaterials.2018.09.015","article-title":"Spatially controlled assembly of affinity ligand and enzyme cargo enables targeting ferritin nanocarriers to caveolae","volume":"185","author":"Shuvaev","year":"2018","journal-title":"Biomaterials"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.biomaterials.2016.05.006","article-title":"Ferritin-mediated siRNA delivery and gene silencing in human tumor and primary cells","volume":"98","author":"Li","year":"2016","journal-title":"Biomaterials"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1430","DOI":"10.1016\/j.biomaterials.2010.09.029","article-title":"Human ferritin cages for imaging vascular macrophages","volume":"32","author":"Terashima","year":"2011","journal-title":"Biomaterials"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"12223","DOI":"10.1021\/ic400484n","article-title":"Ferritin: The protein nanocage and iron biomineral in health and in disease","volume":"52","author":"Theil","year":"2013","journal-title":"Inorg. Chem."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/0005-2728(96)00022-9","article-title":"The ferritins:Molecular properties, iron storage function and cellular regulation","volume":"1275","author":"Harrison","year":"1996","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1126\/science.1636086","article-title":"Magnetoferritin: In vitro synthesis of a novel magnetic protein","volume":"257","author":"Meldrum","year":"1992","journal-title":"Science"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1016\/j.nbt.2014.12.006","article-title":"Ferritin family proteins and their use in bionanotechnology","volume":"32","author":"He","year":"2015","journal-title":"New Biotechnol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"4705","DOI":"10.1021\/nn500454n","article-title":"A smart platform for hyperthermia application in cancer treatment:Cobalt-doped ferrite nanoparticles mineralized in human ferritin cages","volume":"8","author":"Fantechi","year":"2014","journal-title":"ACS Nano"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"6988","DOI":"10.1021\/nn402199g","article-title":"Ferritin nanocages to encapsulate and deliver photosensitizers for efficient photodynamic therapy against cancer","volume":"7","author":"Zhen","year":"2013","journal-title":"ACS Nano"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1038\/s41423-021-00643-6","article-title":"Ferritin nanoparticle-based SARS-CoV-2 RBD vaccine induces a persistent antibody response and long-term memory in mice","volume":"18","author":"Wang","year":"2021","journal-title":"Cell Mol. Immunol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1021\/acscentsci.0c01405","article-title":"A single immunization with spike-functionalized ferritin vaccines elicits neutralizing antibody responses against SARS-CoV-2 in mice","volume":"7","author":"Powell","year":"2021","journal-title":"ACS Cent. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Caixia, Y., Tian, R., Liu, T., and Liu, G. (2016). MRI Reporter Genes for Noninvasive Molecular Imaging. Molecules, 21.","DOI":"10.3390\/molecules21050580"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"4184","DOI":"10.1021\/acsnano.5b07408","article-title":"Bioengineered magnetoferritin nanoprobes for single-dose nuclear-magnetic resonance tumor imaging","volume":"10","author":"Zhao","year":"2016","journal-title":"ACS Nano"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.biomaterials.2016.07.032","article-title":"Targeted iron oxide nanoparticles for the enhancement of radiation therapy","volume":"105","author":"Hauser","year":"2016","journal-title":"Biomaterials"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"7683","DOI":"10.1073\/pnas.89.16.7683","article-title":"Magnetite biomineralization in the human brain","volume":"89","author":"Kirschvink","year":"1992","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"294","DOI":"10.2497\/jjspm.44.294","article-title":"Studies of inorganic crystals in biological tissue:Magnetite in human tumor","volume":"44","author":"Kobayashi","year":"1997","journal-title":"J. Jpn. Soc. Powder Powder Metall."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"5085","DOI":"10.1039\/C6CS00432F","article-title":"Bioinspired synthesis of magnetite nanoparticles","volume":"45","author":"Mirabello","year":"2016","journal-title":"Chem. Soc. Rev."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"3183","DOI":"10.1021\/cm100289d","article-title":"Synthesis of magnetite nanooctahedra and their magnetic field-induced two-\/three-dimensional superstructure","volume":"22","author":"Li","year":"2010","journal-title":"Chem. Mater."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Baumgartner, J., Bertinetti, L., Widdrat, M., Hirt, A.M., and Faivre, D. (2013). Formation of magnetite nanoparticles at low temperature:From superparamagnetic to stable single domain particles. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0057070"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Ganapathe, L.S., Mohamed, M.A., Yunus, R.M., and Berhanuddin, D.D. (2020). Magnetite (Fe3O4) nanoparticles in biomedical application:From synthesis to surface functionalisation. Magnetochemistry, 6.","DOI":"10.3390\/magnetochemistry6040068"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"10332","DOI":"10.1038\/s41598-020-67117-z","article-title":"Iron stored in ferritin is chemically reduced in the presence of aggregating A\u03b2(1\u201342)","volume":"10","author":"Everett","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"\u0160trb\u00e1k, O., Balejc\u00edkov\u00e1, L., Kmetov\u00e1, M., Gombos, J., Trancikova, A., Pokusa, M., and Kopcansk\u00fd, P. (2020). Quantification of iron release from native ferritin and magnetoferritin induced by vitamins B2 and C. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21176332"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.pbiomolbio.2017.09.018","article-title":"On a generalized Levinthal\u2019s paradox:The role of long- and short range interactions in complex bio-molecular reactions, including protein and DNA folding","volume":"132","author":"Melkikh","year":"2018","journal-title":"Prog. Biophys. Mol. Biol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1868","DOI":"10.1126\/science.282.5395.1868","article-title":"Magnetic microstructure of magnetotactic bacteria by electron holography","volume":"282","author":"McCartney","year":"1998","journal-title":"Science"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1007\/s00114-007-0236-0","article-title":"A novel concept of Fe-mineral based magnetoreception:Histological and physicochemical data from the upper beak of homing pigeons","volume":"94","author":"Fleissner","year":"2007","journal-title":"Naturwissenschaften"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"6069","DOI":"10.1021\/jp211843g","article-title":"Formation pathways of magnetite nanoparticles by coprecipitation method","volume":"116","author":"Ahn","year":"2012","journal-title":"J. Phys. Chem. C."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1021\/cm970421o","article-title":"Biomimetic synthesis and characterization of magnetic proteins (magnetoferritin)","volume":"10","author":"Wong","year":"1998","journal-title":"Chem. Mater."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"465707","DOI":"10.1088\/0957-4484\/21\/46\/465707","article-title":"Size-dependent properties of magnetoferritin","volume":"21","author":"Carbonera","year":"2010","journal-title":"Nanotechnology"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.jmmm.2014.02.053","article-title":"Magnetic anisotropy of polycrystalline magnetoferritin investigated by SQUID and electron magnetic resonance","volume":"361","author":"Moro","year":"2014","journal-title":"J. Magn. Magn. Mater."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1006\/abbi.1996.0007","article-title":"Thermal stability of horse spleen apoferritin and human recombinant H apoferritin","volume":"325","author":"Stefanini","year":"1996","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.mencom.2019.05.012","article-title":"Influence of synthesis temperature on structural and magnetic properties of magnetoferritin","volume":"29","author":"Garamus","year":"2019","journal-title":"Mendeleev Commun."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"10852","DOI":"10.1021\/acsnano.5b03607","article-title":"Self-assembly of ferritin nanoparticles into an enzyme nanocomposite with tunable size for ultrasensitive immunoassay","volume":"9","author":"Men","year":"2015","journal-title":"ACS Nano"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"172","DOI":"10.4028\/www.scientific.net\/KEM.855.172","article-title":"Blocking temperature of magnetite nanoparticles Fe3O4 encapsulated silicon dioxide SiO2","volume":"855","author":"Saragi","year":"2020","journal-title":"Key Eng. Mater."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/j.jmmm.2004.11.044","article-title":"Blocking phenomena studies on ferronematics","volume":"289","author":"Saravia","year":"2005","journal-title":"J. Magn. Magn. Mater."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"134022","DOI":"10.1088\/0022-3727\/41\/13\/134022","article-title":"Bioengineered magnetic crystals","volume":"41","author":"Kasyutich","year":"2008","journal-title":"J. Phys. D: Appl. Phys."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"355704","DOI":"10.1088\/0957-4484\/23\/35\/355704","article-title":"The Faraday effect of natural and artificial ferritins","volume":"23","author":"Koralewski","year":"2012","journal-title":"Nanotechnology"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.molliq.2019.03.023","article-title":"Hyperthermic effect in magnetoferritin aqueous colloidal solution","volume":"283","author":"Saksl","year":"2019","journal-title":"J. Mol. Liq."},{"key":"ref_41","first-page":"310","article-title":"Gamma-ray shielding of concretes including magnetite in different rate","volume":"8","author":"Oto","year":"2013","journal-title":"Int. J. Phys. Sci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.jinorgbio.2003.10.001","article-title":"A formula for correlating pKa values determined in D2O and H2O","volume":"98","author":"Krezel","year":"2004","journal-title":"J. Inorg. Biochem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"971","DOI":"10.3367\/UFNe.0180.201010a.1009","article-title":"Small-angle neutron scattering in structure research of magnetic fluids","volume":"53","author":"Avdeev","year":"2010","journal-title":"Phys.\u2013Uspekhi"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"6963","DOI":"10.1016\/S0021-9258(17)32928-9","article-title":"Hydrogen ion interactions of horse spleen ferritin and apoferritin","volume":"251","author":"Stilk","year":"1976","journal-title":"J. Biol. Chem."},{"key":"ref_45","first-page":"115","article-title":"Metaloproteins of the ferritin type","volume":"111","author":"Fodran","year":"2017","journal-title":"Chem. Listy"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1016\/j.colsurfb.2017.05.036","article-title":"The effect of solution pH on the structural stability of magnetoferritin","volume":"156","author":"Garamus","year":"2017","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.jmmm.2014.10.085","article-title":"SANS contrast variation study of magnetoferitin structure at various iron loading","volume":"377","author":"Petrenko","year":"2015","journal-title":"J. Magn. Magn. Mater."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Rosato, E., Bonelli, M., Locatelli, M., de Grazia, U., Tartaglia, A., Savini, F., and D\u2019Ovidio, C. (2021). Forensic Biochemical Markers to Evaluate the Agonal Period: A Literature Review. Molecules, 26.","DOI":"10.3390\/molecules26113259"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"127629","DOI":"10.1016\/j.chemosphere.2020.127629","article-title":"Dechlorination of 2,4,4\u2032-trichlorobiphenyl by magnetoferritin with different loading factors","volume":"260","year":"2020","journal-title":"Chemosphere"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1007\/s00604-013-1105-5","article-title":"Peroxidase-like activity of magnetoferritin","volume":"181","author":"Pospiskova","year":"2014","journal-title":"Microchim. Acta"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Pastore, P., Roverso, M., Tedesco, E., Micheletto, M., Mantovan, E., Zanella, M., and Benetti, F. (2020). Comparative Evaluation of Intestinal Absorption and Functional Value of Iron Dietary Supplements and Drug with Different Delivery Systems. Molecules, 25.","DOI":"10.3390\/molecules25245989"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"3988","DOI":"10.1016\/j.biomaterials.2011.02.031","article-title":"Long term in vivo biotransformation of iron oxide nanoparticles","volume":"32","author":"Levy","year":"2011","journal-title":"Biomaterials"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.jcis.2007.02.058","article-title":"Redox equilibria of iron oxides in aqueous-based magnetite dispersions: Effect of pH and redox potential","volume":"311","author":"Pang","year":"2007","journal-title":"J. Colloid Interface Sci."},{"key":"ref_54","unstructured":"(2021, November 15). Match!-Phase Analysis Using Powder Diffraction, Crystal Impact-Dr. Putz, H. & Dr. Brandenburg, K. GbR, Kreuzherrenstr. 102, 53227 Bonn, Germany. Available online: https:\/\/www.crystalimpact.de\/match."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"D420","DOI":"10.1093\/nar\/gkr900","article-title":"Crystallography Open Database (COD): An open-access collection of crystal structures and platform for World-Wide Collaboration","volume":"40","author":"Merkys","year":"2012","journal-title":"Nucleic Acids Res."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/26\/22\/6960\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:32:23Z","timestamp":1760167943000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/26\/22\/6960"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,18]]},"references-count":55,"journal-issue":{"issue":"22","published-online":{"date-parts":[[2021,11]]}},"alternative-id":["molecules26226960"],"URL":"https:\/\/doi.org\/10.3390\/molecules26226960","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,18]]}}}