{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T14:22:33Z","timestamp":1770819753662,"version":"3.50.1"},"reference-count":30,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2021,11,22]],"date-time":"2021-11-22T00:00:00Z","timestamp":1637539200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>We aimed to evaluate the anticancer potential of crude venom (CV), \u03b3 irradiated Certastes cerastes venom (IRRV), and propolis ethanolic extract (PEE). IRRV showed a higher toxicity than CV, while CV-PEE showed higher toxicity than IRRV and CV against lung [A549] and prostate [PC3] cancer cells. Toxicity to [A549] and [PC3] cells was concentration and cell type dependent. In comparison to controls, apoptotic genes showed a significant upregulation of P53 and Casp-3 and a downregulation of Bcl-2. Also, induced elevated DNA accumulation in the [S] phase post PC3 cell treatment with IRRV and CV, as well as a significant DNA accumulation at G2\/M phase after IRRV treatment of A549 cells. In contrast, PC3 cells showed a negligible cellular DNA accumulation after PEE treatment. Glutathione reductase [GR] was reduced in case of PC3 and A549 cell treated with IRRV, CV, and PEE compared with its values in untreated cell control. The Malondialdehyde [MDA] values in both cells recorded a significant elevation post IRRV treatment compared to the rest of the treatment regimen and untreated cell control. Similarly, IRRV and CV-PEE mix showed obviously higher reactive oxygen species [ROS] values than PC3 and A549 cell treatments with CV and PEE.<\/jats:p>","DOI":"10.3390\/molecules26227057","type":"journal-article","created":{"date-parts":[[2021,11,30]],"date-time":"2021-11-30T22:01:11Z","timestamp":1638309671000},"page":"7057","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Evaluation of the Anticancer Potential of Crude, Irradiated Cerastes cerastes Snake Venom and Propolis Ethanolic Extract &amp; Related Biological Alterations"],"prefix":"10.3390","volume":"26","author":[{"given":"Mostafa I.","family":"Abdelglil","sequence":"first","affiliation":[{"name":"Faculty of Sciences, Cairo University, Giza 12613, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanaa O.","family":"Abdallah","sequence":"additional","affiliation":[{"name":"Faculty of Sciences, Cairo University, Giza 12613, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed A.","family":"El-Desouky","sequence":"additional","affiliation":[{"name":"Faculty of Sciences, Cairo University, Giza 12613, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4310-3539","authenticated-orcid":false,"given":"Mohammad Y.","family":"Alfaifi","sequence":"additional","affiliation":[{"name":"Biology Department, Faculty of Science, King Khalid University, Abha 9004, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4680-3215","authenticated-orcid":false,"given":"Serag Eldin I.","family":"Elbehairi","sequence":"additional","affiliation":[{"name":"Biology Department, Faculty of Science, King Khalid University, Abha 9004, Saudi Arabia"},{"name":"Cell Culture Lab, Egyptian Organization for Biological Products and Vaccines (VACSERA Holding Company), 51 Wezaret El-Zeraa St., Agouza, Giza 12654, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aly F.","family":"Mohamed","sequence":"additional","affiliation":[{"name":"The International Center for Training & Advanced Researches (ICTAR\u2013Egypt), Cairo 11647, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,11,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1007\/s10616-014-9820-2","article-title":"Evaluation of cytotoxic activities of snake venoms toward breast (MCF-7) and skin cancer (A-375) cell lines","volume":"68","author":"Bradshaw","year":"2016","journal-title":"Cytotechnology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"204","DOI":"10.3389\/fonc.2017.00204","article-title":"Cancer: Linking powerhouses to suicidal bags","volume":"7","author":"Nasrallah","year":"2017","journal-title":"Front. 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