{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T01:51:07Z","timestamp":1769910667966,"version":"3.49.0"},"reference-count":64,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2021,12,5]],"date-time":"2021-12-05T00:00:00Z","timestamp":1638662400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001804","name":"Canada Research Chairs","doi-asserted-by":"publisher","award":["950-232164"],"award-info":[{"award-number":["950-232164"]}],"id":[{"id":"10.13039\/501100001804","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Amaryllidaceae plants are rich in alkaloids with biological properties. Pancratium trianthum is an Amaryllidaceae species widely used in African folk medicine to treat several diseases such as central nervous system disorders, tumors, and microbial infections, and it is used to heal wounds. The current investigation explored the biological properties of alkaloid extracts from bulbs of P. trianthum collected in the Senegalese flora. Alkaloid extracts were analyzed and identified by chromatography and mass spectrometry. Alkaloid extracts from P. trianthum displayed pleiotropic biological properties. Cytotoxic activity of the extracts was determined on hepatocarcinoma Huh7 cells and on acute monocytic leukemia THP-1 cells, while agar diffusion and microdilution assays were used to evaluate antibacterial activity. Antiviral activity was measured by infection of extract-treated cells with dengue virus (DENVGFP) and human immunodeficiency virus-1 (HIV-1GFP) reporter vectors. Cytotoxicity and viral inhibition were the most striking of P. trianthum\u2019s extract activities. Importantly, non-cytotoxic concentrations were highly effective in completely preventing DENVGFP replication and in reducing pseudotyped HIV-1GFP infection levels. Our results show that P. trianthum is a rich source of molecules for the potential discovery of new treatments against various diseases. Herein, we provide scientific evidence to rationalize the traditional uses of P. trianthum for wound treatment as an anti-dermatosis and antiseptic agent.<\/jats:p>","DOI":"10.3390\/molecules26237382","type":"journal-article","created":{"date-parts":[[2021,12,6]],"date-time":"2021-12-06T03:10:38Z","timestamp":1638760238000},"page":"7382","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Biological Investigation of Amaryllidaceae Alkaloid Extracts from the Bulbs of Pancratium trianthum Collected in the Senegalese Flora"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7697-4088","authenticated-orcid":false,"given":"Seydou","family":"Ka","sequence":"first","affiliation":[{"name":"D\u00e9partement de Chimie, Biochimie et Physique, Universit\u00e9 du Qu\u00e9bec \u00e0 Trois-Rivi\u00e8res, Trois-Rivi\u00e8res, QC G8Z 4M3, Canada"},{"name":"Laboratoire de Chimie Organique et Th\u00e9rapeutique, Facult\u00e9 de M\u00e9decine, de Pharmacie, et d\u2019Odontologie de Dakar, Dakar B.P. 5005, Senegal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6235-6465","authenticated-orcid":false,"given":"Natacha","family":"M\u00e9rindol","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Chimie, Biochimie et Physique, Universit\u00e9 du Qu\u00e9bec \u00e0 Trois-Rivi\u00e8res, Trois-Rivi\u00e8res, QC G8Z 4M3, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8544-8741","authenticated-orcid":false,"given":"Insa","family":"Seck","sequence":"additional","affiliation":[{"name":"Laboratoire de Chimie Organique et Th\u00e9rapeutique, Facult\u00e9 de M\u00e9decine, de Pharmacie, et d\u2019Odontologie de Dakar, Dakar B.P. 5005, Senegal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7888-9581","authenticated-orcid":false,"given":"Simon","family":"Ricard","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Chimie, Biochimie et Physique, Universit\u00e9 du Qu\u00e9bec \u00e0 Trois-Rivi\u00e8res, Trois-Rivi\u00e8res, QC G8Z 4M3, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdoulaye","family":"Diop","sequence":"additional","affiliation":[{"name":"Laboratoire Bact\u00e9riologie-Virologie, CHU Aristide Le Dantec, Universit\u00e9 Cheikh Anta Diop de Dakar, Dakar B.P. 5005, Senegal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheikh Saad Bouh","family":"Boye","sequence":"additional","affiliation":[{"name":"Laboratoire Bact\u00e9riologie-Virologie, CHU Aristide Le Dantec, Universit\u00e9 Cheikh Anta Diop de Dakar, Dakar B.P. 5005, Senegal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karima","family":"Landelouci","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Biologie M\u00e9dicale, Universit\u00e9 du Qu\u00e9bec \u00e0 Trois-Rivi\u00e8res, Trois-Rivi\u00e8res, QC G8Z 4M3, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6486-3647","authenticated-orcid":false,"given":"Benoit","family":"Daoust","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Chimie, Biochimie et Physique, Universit\u00e9 du Qu\u00e9bec \u00e0 Trois-Rivi\u00e8res, Trois-Rivi\u00e8res, QC G8Z 4M3, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7776-5058","authenticated-orcid":false,"given":"Lionel","family":"Berthoux","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Biologie M\u00e9dicale, Universit\u00e9 du Qu\u00e9bec \u00e0 Trois-Rivi\u00e8res, Trois-Rivi\u00e8res, QC G8Z 4M3, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Genevi\u00e8ve","family":"P\u00e9pin","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Biologie M\u00e9dicale, Universit\u00e9 du Qu\u00e9bec \u00e0 Trois-Rivi\u00e8res, Trois-Rivi\u00e8res, QC G8Z 4M3, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0082-5817","authenticated-orcid":false,"given":"Matar","family":"Seck","sequence":"additional","affiliation":[{"name":"Laboratoire de Chimie Organique et Th\u00e9rapeutique, Facult\u00e9 de M\u00e9decine, de Pharmacie, et d\u2019Odontologie de Dakar, Dakar B.P. 5005, Senegal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4355-5503","authenticated-orcid":false,"given":"Isabel","family":"Desgagn\u00e9-Penix","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Chimie, Biochimie et Physique, 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Prod. Rep."},{"key":"ref_2","first-page":"1934578X1300800938","article-title":"Alkaloids of the South African Amaryllidaceae: A review","volume":"8","author":"Nair","year":"2013","journal-title":"Nat. Prod. Commun."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Meerow, A., and Snijman, D. (1998). Amaryllidaceae. Flowering Plants\u00b7Monocotyledons, Springer.","DOI":"10.1007\/978-3-662-03533-7_11"},{"key":"ref_4","unstructured":"Kerharo, J., and Bouquet, A. (1950). Plantes m\u00e9dicinales et toxiques de la C\u00f4te d\u2019Ivoire-Haute-Volta: Mission d\u2019\u00e9tude de la pharmacop\u00e9e indig\u00e8ne en AOF, Editions Vigot."},{"key":"ref_5","unstructured":"Kerharo, J., and Adam, J.-G. (1974). La pharmacop\u00e9e s\u00e9n\u00e9galaise traditionnelle: Plantes m\u00e9dicinales et toxiques, Editions Vigot fr\u00e8res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1982","DOI":"10.1021\/cr078198u","article-title":"Chemistry, biology, and medicinal potential of narciclasine and its congeners","volume":"108","author":"Kornienko","year":"2008","journal-title":"Chem. Rev."},{"key":"ref_7","first-page":"1255","article-title":"GC\/MS analysis of three Amaryllidaceae species and their cholinesterase activity","volume":"6","author":"Opletal","year":"2011","journal-title":"Nat. Prod. Commun."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1016\/j.jep.2012.01.037","article-title":"Antibacterial activity of crinane alkaloids from Boophone disticha (Amaryllidaceae)","volume":"140","author":"Cheesman","year":"2012","journal-title":"J. Ethnopharmacol."},{"key":"ref_9","first-page":"479","article-title":"The effect of Amaryllidaceae alkaloids haemanthamine and haemanthidine on cell cycle progression and apoptosis in p53-negative human leukemic Jurkat cells","volume":"21","author":"Havelek","year":"2014","journal-title":"Phytomed. Int. J. Phytother. Phytopharm."},{"key":"ref_10","first-page":"1587","article-title":"The Biological Activity of Alkaloids from the Amaryllidaceae: From Cholinesterases Inhibition to Anticancer Activity","volume":"11","author":"Havelek","year":"2016","journal-title":"Nat. Prod. Commun."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Cahl\u00edkov\u00e1, L., Van\u011b\u010dkov\u00e1, N., \u0160afratov\u00e1, M., Breiterov\u00e1, K., Blunden, G., Hulcov\u00e1, D., and Opletal, L. (2019). The Genus Nerine Herb. (Amaryllidaceae): Ethnobotany, Phytochemistry, and Biological Activity. Molecules, 24.","DOI":"10.3390\/molecules24234238"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"112055","DOI":"10.1016\/j.phytochem.2019.112055","article-title":"Amaryllidaceae alkaloids from Narcissus pseudonarcissus L. cv. Dutch Master as potential drugs in treatment of Alzheimer\u2019s disease","volume":"165","author":"Hulcova","year":"2019","journal-title":"Phytochemistry"},{"key":"ref_13","first-page":"154","article-title":"Alkaloid profiling of Hippeastrum cultivars by GC-MS, isolation of Amaryllidaceae alkaloids and evaluation of their cytotoxicity","volume":"14","author":"Havelek","year":"2020","journal-title":"Rec. Nat. Prod."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Cahl\u00edkov\u00e1, L., Breiterov\u00e1, K., and Opletal, L. (2020). Chemistry and Biological Activity of Alkaloids from the Genus Lycoris (Amaryllidaceae). Molecules, 25.","DOI":"10.3390\/molecules25204797"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Koutov\u00e1, D., Maafi, N., Havelek, R., Opletal, L., Blunden, G., \u0158ez\u00e1\u010dov\u00e1, M., and Cahl\u00edkov\u00e1, L. (2020). Chemical and Biological Aspects of Montanine-Type Alkaloids Isolated from Plants of the Amaryllidaceae Family. Molecules, 25.","DOI":"10.3390\/molecules25102337"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Marikova, J., Mamun, A.A., Shammari, L.A., Korabecny, J., Kucera, T., Hulcova, D., Kunes, J., Malanik, M., Vaskova, M., and Kohelova, E. (2021). Structure Elucidation and Cholinesterase Inhibition Activity of Two New Minor Amaryllidaceae Alkaloids. Molecules, 26.","DOI":"10.3390\/molecules26051279"},{"key":"ref_17","unstructured":"Ba, D. (2020, November 25). Le Pancratium trianthum. Available online: http:\/\/seyilaabe-htkm.blogspot.com\/2015\/10\/."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/S0367-326X(00)00250-1","article-title":"A methylflavan with free radical scavenging properties from Pancratium littorale","volume":"72","author":"Ioset","year":"2001","journal-title":"Fitoterapia"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1016\/j.indcrop.2012.03.028","article-title":"Variation of the chemical composition of floral volatiles in the endangered Tunisian Pancratium maritimum L. populations (Amaryllidaceae)","volume":"40","author":"Sanaa","year":"2012","journal-title":"Ind. Crops Prod."},{"key":"ref_20","unstructured":"Berhaut, J. (1967). Flora of Senegal, Editions Clairafrique."},{"key":"ref_21","unstructured":"Schultes, E.R. (1978). Atlas des Plantes Hallucinog\u00e8nes du Monde, Editions de l\u2019Aurore."},{"key":"ref_22","unstructured":"Monteil, V. (1953). Contribution \u00e0 l\u2019\u00e9tude de la flore du Sahara occidental II. Institut des Hautes Etudes Marocaines, \u00c9ditions Larose. Notes Documents VI."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1007\/s11101-020-09678-5","article-title":"Biosynthesis of alkaloids in Amaryllidaceae plants: A review","volume":"20","year":"2021","journal-title":"Phytochem. Rev."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Ka, S., Merindol, N., Koirala, M., and Desgagne-Penix, I. (2020). Biosynthesis and biological activities of newly discovered Amaryllidaceae alkaloids. Molecules, 25.","DOI":"10.3390\/molecules25214901"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"418","DOI":"10.2174\/1568026616666160824104052","article-title":"Heterocyclic Amaryllidaceae Alkaloids: Biosynthesis and Pharmacological Applications","volume":"17","author":"Hotchandani","year":"2017","journal-title":"Curr. Top. Med. Chem."},{"key":"ref_26","first-page":"157","article-title":"Galanthamine from Galanthus and other Amaryllidaceae\u2013chemistry and biology based on traditional use","volume":"Volume 68","author":"Heinrich","year":"2010","journal-title":"The Alkaloids: Chemistry and Biology"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"112305","DOI":"10.1016\/j.jep.2019.112305","article-title":"New role for crinamine as a potent, safe and selective inhibitor of human monoamine oxidase B: In vitro and in silico pharmacology and modeling","volume":"248","author":"Naidoo","year":"2020","journal-title":"J. Ethnopharmacol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"e0039821","DOI":"10.1128\/AAC.00398-21","article-title":"Amaryllidaceae alkaloid cherylline inhibits the replication of dengue and Zika viruses","volume":"65","author":"Ka","year":"2021","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"112390","DOI":"10.1016\/j.phytochem.2020.112390","article-title":"Gigantelline, gigantellinine and gigancrinine, cherylline- and crinine-type alkaloids isolated from Crinum jagus with anti-acetylcholinesterase activity","volume":"175","author":"Ka","year":"2020","journal-title":"Phytochemistry"},{"key":"ref_30","first-page":"1","article-title":"Chemistry and biology of Pancratium alkaloids","volume":"Volume 68","author":"Ravelo","year":"2010","journal-title":"The Alkaloids: Chemistry and Biology"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1007\/BF00579673","article-title":"Alkaloid composition of Pancratium trianthum","volume":"18","author":"Frederik","year":"1983","journal-title":"Chem. Nat. Compd."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"(1996). Chapter 1 Occurrence of Alkaloids in Plant Species. Chem. Nat. Compd., 32, 104\u2013173.","DOI":"10.1007\/BF03327478"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1111\/j.1095-8339.2012.01268.x","article-title":"Phylogenetic and biogeographical inferences for Pancratium (Amaryllidaceae), with an emphasis on the Mediterranean species based on plastid sequence data","volume":"170","author":"Brullo","year":"2012","journal-title":"Bot. J. Linn. Soc."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"W465","DOI":"10.1093\/nar\/gkn180","article-title":"Phylogeny. fr: Robust phylogenetic analysis for the non-specialist","volume":"36","author":"Dereeper","year":"2008","journal-title":"Nucleic Acids Res."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Tallini, L.R., Andrade, J.P., Kaiser, M., Viladomat, F., Nair, J.J., Zuanazzi, J.A.S., and Bastida, J. (2017). Alkaloid Constituents of the Amaryllidaceae Plant Amaryllis belladonna L.. Molecules, 22.","DOI":"10.3390\/molecules22091437"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1002\/pca.2865","article-title":"In vitro micropropagation and alkaloids analysis by GC\u2013MS of Chilean Amaryllidaceae plants: Rhodophiala pratensis","volume":"31","author":"Bustamante","year":"2020","journal-title":"Phytochem. Anal."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2521","DOI":"10.1016\/S0024-3205(02)02034-9","article-title":"Acetylcholinesterase inhibitory activity of some Amaryllidaceae alkaloids and Narcissus extracts","volume":"71","author":"Bastida","year":"2002","journal-title":"Life Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1039\/b508966m","article-title":"Acetylcholinesterase inhibitors from plants and fungi","volume":"23","author":"Houghton","year":"2006","journal-title":"Nat. Prod. Rep."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2893","DOI":"10.1016\/j.phytochem.2004.08.052","article-title":"Choline esterase inhibitory properties of alkaloids from two Nigerian Crinum species","volume":"65","author":"Houghton","year":"2004","journal-title":"Phytochemistry"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1023\/B:CONC.0000003421.65467.9c","article-title":"Bioactivity-directed fractionation of alkaloids from some Amaryllidaceae plants and their anticholinesterase activity","volume":"39","author":"Orhan","year":"2003","journal-title":"Chem. Nat. Compd."},{"key":"ref_41","first-page":"19","article-title":"Egyptian Pancratium maritimum L. flowers as a source of anti-Alzheimer\u2019s agents","volume":"53","author":"Soltan","year":"2015","journal-title":"Bull. Fac. Pharm. Cairo Univ."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"6244","DOI":"10.1021\/jm901031h","article-title":"Lycorine, the main phenanthridine Amaryllidaceae alkaloid, exhibits significant antitumor activity in cancer cells that display resistance to proapoptotic stimuli: An investigation of structure\u2212activity relationship and mechanistic insight","volume":"52","author":"Andolfi","year":"2009","journal-title":"J. Med. Chem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"41","DOI":"10.2174\/138955710791112604","article-title":"Lycorine and its derivatives for anticancer drug design","volume":"10","author":"Decaestecker","year":"2010","journal-title":"Mini-Rev. Med. Chem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1186\/1472-6882-14-409","article-title":"Relevant and selective activity of Pancratium illyricum L. against Candida albicans clinical isolates: A combined effect on yeast growth and virulence","volume":"14","author":"Bonvicini","year":"2014","journal-title":"BMC Complement. Altern. Med."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"303","DOI":"10.3109\/13880209209054019","article-title":"Antibacterial alkaloids in Crinum jagus","volume":"30","author":"Adesanya","year":"1992","journal-title":"Int. J. Pharmacogn."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"5977","DOI":"10.1021\/jf200452z","article-title":"Activity of lycorine analogues against the fish bacterial pathogen Flavobacterium columnare","volume":"59","author":"Tan","year":"2011","journal-title":"J. Agric. Food Chem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2113","DOI":"10.1016\/j.phytochem.2004.03.020","article-title":"(\u2212)-Amarbellisine, a lycorine-type alkaloid from Amaryllis belladonna L. growing in Egypt","volume":"65","author":"Evidente","year":"2004","journal-title":"Phytochemistry"},{"key":"ref_48","unstructured":"UNAIDS (2021, November 03). Country Factsheets SENEGAL. Available online: https:\/\/www.unaids.org\/sites\/default\/files\/media_asset\/2020_aids-data-book_en.pdf."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"8768","DOI":"10.1128\/JVI.00768-14","article-title":"Deep sequencing of HIV-infected cells: Insights into nascent transcription and host-directed therapy","volume":"88","author":"Peng","year":"2014","journal-title":"J. Virol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1055\/s-2004-827239","article-title":"Alkaloids from Leucojum vernum and antiretroviral activity of Amaryllidaceae alkaloids","volume":"70","author":"Szlavik","year":"2004","journal-title":"Planta Med."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1007\/s11418-013-0767-y","article-title":"Potential anti-dengue medicinal plants: A review","volume":"67","author":"Yaakob","year":"2013","journal-title":"J. Nat. Med."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1522","DOI":"10.1002\/cmdc.201300505","article-title":"Anti-dengue-virus activity and structure\u2013activity relationship studies of lycorine derivatives","volume":"9","author":"Wang","year":"2014","journal-title":"ChemMedChem"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.virol.2020.04.009","article-title":"Antiviral activity of lycorine against Zika virus in vivo and in vitro","volume":"546","author":"Chen","year":"2020","journal-title":"Virology"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1016\/j.virol.2008.11.003","article-title":"A single-amino acid substitution in West Nile virus 2K peptide between NS4A and NS4B confers resistance to lycorine, a flavivirus inhibitor","volume":"384","author":"Zou","year":"2009","journal-title":"Virology"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.phytochem.2014.03.007","article-title":"Crinine-type alkaloids from Hippeastrum aulicum and H. calyptratum","volume":"103","author":"Guo","year":"2014","journal-title":"Phytochemistry"},{"key":"ref_56","first-page":"1","article-title":"Extraction, isolation and characterization of bioactive compounds from plants\u2019 extracts","volume":"8","author":"Sasidharan","year":"2011","journal-title":"Afr. J. Tradit. Complement. Altern. Med."},{"key":"ref_57","first-page":"178","article-title":"Modified Dragendorff reagent for the procurement of lasting coloration of spots in paper chromatographic and electrophoretic investigation of alkaloids","volume":"94","author":"Robles","year":"1959","journal-title":"Pharm. Weekbl."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"17356","DOI":"10.1038\/s41598-017-17724-0","article-title":"Transcriptome and metabolome profiling of Narcissus pseudonarcissus \u2018King Alfred\u2019 reveal components of Amaryllidaceae alkaloid metabolism","volume":"7","author":"Singh","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Tallini, L.R., Torras-Claveria, L., Borges, W.S., Kaiser, M., Viladomat, F., Zuanazzi, J.A.S., and Bastida, J. (2018). N-oxide alkaloids from Crinum amabile (Amaryllidaceae). Molecules, 23.","DOI":"10.3390\/molecules23061277"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1006\/jmbi.1994.1651","article-title":"Domains of interaction between alpha interferon and its receptor components","volume":"243","author":"Monneron","year":"1994","journal-title":"J. Mol. Biol."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Fischl, W., and Bartenschlager, R. (2013). High-throughput screening using dengue virus reporter genomes. Antiviral Methods and Protocols, Springer.","DOI":"10.1007\/978-1-62703-484-5_17"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1038\/385645a0","article-title":"CCR3 and CCR5 are co-receptors for HIV-1 infection of microglia","volume":"385","author":"He","year":"1997","journal-title":"Nature"},{"key":"ref_63","first-page":"531","article-title":"Phytochemical and Biological Investigation of Clivia nobilis Flowers Cultivated in Egypt","volume":"15","author":"Shawky","year":"2016","journal-title":"Iran. J. Pharm. Res. IJPR"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.jpha.2015.11.005","article-title":"Methods for in vitro evaluating antimicrobial activity: A review","volume":"6","author":"Balouiri","year":"2016","journal-title":"J. Pharm. 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