{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T21:08:04Z","timestamp":1789420084335,"version":"build-2803163510"},"reference-count":53,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2021,12,17]],"date-time":"2021-12-17T00:00:00Z","timestamp":1639699200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The increase in antibiotic resistance and the emergence of new bacterial infections have intensified the research for natural products from plants with associated therapy. This study aimed to verify the antibacterial and antioxidant activity of crude extracts of the genus Plectranthus species, being the first report on the modulation of aminoglycosides antibiotic activity by Plectranthus amboinicus extracts. The chemical composition was obtained by chemical prospecting and High-Performance Liquid Chromatography with diode arrangement detector (HPLC\/DAD). The antibacterial activities of the extracts alone or in association with aminoglycosides were analyzed using the microdilution test. The antioxidant activity was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging. The phytochemical prospection allowed the flavonoids, saponins, tannins and triterpenoids to be identified. Quercetin, rutin, gallic acid, chlorogenic acid, caffeic acid, catechin, kaempferol, glycosylated kaempferol, quercitrin, and isoquercitrin were identified and quantified. The principal component analysis (PCA) observed the influence of flavonoids and phenolic acids from Plectranthus species on studied activities. Phytochemical tests with the extracts indicated, especially, the presence of flavonoids, confirmed by quantitative analysis by HPLC. The results revealed antibacterial activities, and synergistic effects combined with aminoglycosides, as well as antioxidant potential, especially for P. ornatus species, with IC50 of 32.21 \u00b5g\/mL. Multivariate analyzes show that the inclusion of data from the antioxidant and antibacterial activity suggests that the antioxidant effect of these species presents a significant contribution to the synergistic effect of phytoconstituents, especially based on the flavonoid contents. The results of this study suggest the antibacterial activity of Plectranthus extracts, as well as their potential in modifying the resistance of the analyzed aminoglycosides.<\/jats:p>","DOI":"10.3390\/molecules26247665","type":"journal-article","created":{"date-parts":[[2021,12,20]],"date-time":"2021-12-20T02:50:18Z","timestamp":1639968618000},"page":"7665","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["HPLC\/DAD, Antibacterial and Antioxidant Activities of Plectranthus Species (Lamiaceae) Combined with the Chemometric Calculations"],"prefix":"10.3390","volume":"26","author":[{"given":"Fab\u00edola F. G.","family":"Rodrigues","sequence":"first","affiliation":[{"name":"Northeast Biotechnology Network, Postgraduate Program in Biotechnology, State University of Cear\u00e1, Fortaleza 60740-000, Brazil"},{"name":"Health Unit, University Center Dr. Le\u00e3o Sampaio, Juazeiro do Norte 63040-000, Brazil"},{"name":"Department of Biological Chemistry, Regional University of Cariri, Crato 63105-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aline A.","family":"Boligon","sequence":"additional","affiliation":[{"name":"Department of Industrial Pharmacy, Federal University of Santa Maria, Santa Maria 97105-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1065-9581","authenticated-orcid":false,"given":"Irwin R. A.","family":"Menezes","sequence":"additional","affiliation":[{"name":"Northeast Biotechnology Network, Postgraduate Program in Biotechnology, State University of Cear\u00e1, Fortaleza 60740-000, Brazil"},{"name":"Department of Biological Chemistry, Regional University of Cariri, Crato 63105-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0137-9441","authenticated-orcid":false,"given":"F\u00e1bio F.","family":"Galv\u00e3o-Rodrigues","sequence":"additional","affiliation":[{"name":"Department of Biological Chemistry, Regional University of Cariri, Crato 63105-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0908-0919","authenticated-orcid":false,"given":"Gerson J. 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R.","family":"Caldas","sequence":"additional","affiliation":[{"name":"Health Unit, University Center Dr. Le\u00e3o Sampaio, Juazeiro do Norte 63040-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6634-4207","authenticated-orcid":false,"given":"Henrique D. M.","family":"Coutinho","sequence":"additional","affiliation":[{"name":"Northeast Biotechnology Network, Postgraduate Program in Biotechnology, State University of Cear\u00e1, Fortaleza 60740-000, Brazil"},{"name":"Department of Biological Chemistry, Regional University of Cariri, Crato 63105-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abolghasem","family":"Siyadatpanah","sequence":"additional","affiliation":[{"name":"Ferdows School of Paramedical and Health, Birjand University of Medical Sciences, Birjand 9717853577, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8678-156X","authenticated-orcid":false,"given":"Bonglee","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Patology, College of Korean Medicine, Kyung Hee University, Seoul 02447, Korea"},{"name":"Korean Medicine-Based Drug Repositioning Cancer Research Center, College of Korean Medicine, Kyung Hee University, Seoul 02447, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 G. M.","family":"Costa","sequence":"additional","affiliation":[{"name":"Northeast Biotechnology Network, Postgraduate Program in Biotechnology, State University of Cear\u00e1, Fortaleza 60740-000, Brazil"},{"name":"Department of Biological Chemistry, Regional University of Cariri, Crato 63105-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana R. 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