{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T02:51:54Z","timestamp":1790909514726,"version":"4.1.0"},"reference-count":34,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2021,12,30]],"date-time":"2021-12-30T00:00:00Z","timestamp":1640822400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"This research was funded by Project for Science and Development plan for Jilin prov-ince (NO: 202110401123YY)","award":["NO: 202110401123YY"],"award-info":[{"award-number":["NO: 202110401123YY"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>This study focused on developing Panos nanoemulsion (P-NE) and enhancing the anti-inflammatory efficacy for the treatment of inflammation. The effects of P-NE were evaluated in terms of Nitric oxide (NO production) in Lipopolysaccharide (LPS), induced RAW 264.7 cells, Reactive oxygen species (ROS) generation using Human Keratinocyte cells (HaCaT), and quantitative polymerase chain reaction (qPCR) analysis. Sea buckthorn oil, Tween 80, and span 80 were used and optimize the process. Panos extract (P-Ext) was prepared using the fermentation process. Further high-energy ultra-sonication was used for the preparation of P-NE. The developed nanoemulsion (NE) was characterized using different analytical methods. Field emission transmission electron microscopy (FE-TEM) analyzed the spherical shape and morphology. In addition, stability was analyzed by Dynamic light scattering (DLS) analysis, where particle size was analyzed 83 nm, and Zeta potential \u221228.20 \u00b1 2 (mV). Furthermore, 90 days of stability was tested using different temperatures conditions where excellent stability was observed. P-NE are non-toxic in (HaCaT), and RAW264.7 cells up to 100 \u00b5g\/mL further showed effects on ROS and NO production of the cells at 50 \u00b5g\/mL. The qPCR analysis demonstrated the suppression of pro-inflammatory mediators for (Cox 2, IL-6, IL-1\u03b2, and TNF-\u03b1, NF-\u03baB, Ikk\u03b1, and iNOS) gene expression. The prepared NE exhibited anti-inflammatory effects, demonstrating its potential as a safe and non-toxic nanomedicine.<\/jats:p>","DOI":"10.3390\/molecules27010218","type":"journal-article","created":{"date-parts":[[2021,12,30]],"date-time":"2021-12-30T23:29:07Z","timestamp":1640906947000},"page":"218","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Panos-Fermented Extract-Mediated Nanoemulsion: Preparation, Characterization, and In Vitro Anti-Inflammatory Effects on RAW 264.7 Cells"],"prefix":"10.3390","volume":"27","author":[{"given":"Rui","family":"Zhang","sequence":"first","affiliation":[{"name":"State Local Joint Engineering Research Center of Ginseng Breeding and Application, Jilin Agricultural University, Changchun 130118, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1334-3876","authenticated-orcid":false,"given":"Esrat Jahan","family":"Rupa","sequence":"additional","affiliation":[{"name":"Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung Hee University, Yongin-si 17104, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siwen","family":"Zheng","sequence":"additional","affiliation":[{"name":"State Local Joint Engineering Research Center of Ginseng Breeding and Application, Jilin Agricultural University, Changchun 130118, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinnatun","family":"Nahar","sequence":"additional","affiliation":[{"name":"Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung Hee University, Yongin-si 17104, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2394-4728","authenticated-orcid":false,"given":"Deok Chun","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung Hee University, Yongin-si 17104, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0790-2237","authenticated-orcid":false,"given":"Se Chan","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung Hee University, Yongin-si 17104, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingping","family":"Wang","sequence":"additional","affiliation":[{"name":"State Local Joint Engineering Research Center of Ginseng Breeding and Application, Jilin Agricultural University, Changchun 130118, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,12,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1012","DOI":"10.1007\/s11626-016-0057-6","article-title":"Silver nanoparticles from Dendropanax morbifera L\u00e9veille inhibit cell migration, induce apoptosis, and increase generation of reactive oxygen species in A549 lung cancer cells","volume":"52","author":"Aceituno","year":"2016","journal-title":"Vitr. Cell. Dev. Biol. Anim."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Mathiyalagan, R., Wang, C., Kim, Y.J., Castro-Aceituno, V., Ahn, S., Subramaniyam, S., Simu, S.Y., Jim\u00e9nez-P\u00e9rez, Z.E., Yang, D.C., and Jung, S.-K. (2019). Preparation of polyethylene glycol-ginsenoside Rh1 and Rh2 conjugates and their efficacy against lung cancer and inflammation. Molecules, 24.","DOI":"10.3390\/molecules24234367"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"172","DOI":"10.4047\/jap.2016.8.3.172","article-title":"Antimicrobial, Anti-oxidant and Cytotoxic Activities of Dendropanax morbifera L\u00e9veille extract for mouthwash and denture cleaning solution","volume":"8","author":"Kim","year":"2016","journal-title":"J. Adv. Prosthodont."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1007\/s10787-019-00630-4","article-title":"Pharmacological effects of ginseng on infectious diseases","volume":"27","author":"Nguyen","year":"2019","journal-title":"Inflammopharmacology"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1016\/j.bse.2009.06.004","article-title":"Chemical composition and larvicidal effects of essential oil of Dendropanax morbifera against Aedes aegypti L.","volume":"37","author":"Chung","year":"2009","journal-title":"Biochem. Syst. Ecol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"3691","DOI":"10.2147\/IJN.S97181","article-title":"Rapid green synthesis of silver and gold nanoparticles using Dendropanax morbifera leaf extract and their anticancer activities","volume":"11","author":"Kim","year":"2016","journal-title":"Int. J. Nanomed."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.jbiosc.2014.12.012","article-title":"Anti-thrombotic effect of rutin isolated from Dendropanax morbifera Leveille","volume":"120","author":"Choi","year":"2015","journal-title":"J. Biosci. Bioeng."},{"key":"ref_8","first-page":"2087","article-title":"Anti-inflammatory effects of Dendropanax morbifera in lipopolysaccharide-stimulated RAW264. 7 macrophages and in an animal model of atopic dermatitis","volume":"19","author":"Choo","year":"2019","journal-title":"Mol. Med. Rep."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.biopha.2018.01.050","article-title":"Caspase-3\/MAPK pathways as main regulators of the apoptotic effect of the phyto-mediated synthesized silver nanoparticle from dried stem of Eleutherococcus senticosus in human cancer cells","volume":"99","author":"Kim","year":"2018","journal-title":"Biomed. Pharmacother."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.jcis.2014.03.035","article-title":"Optimization of isothermal low-energy nanoemulsion formation: Hydrocarbon oil, non-ionic surfactant, and water systems","volume":"425","author":"Komaiko","year":"2014","journal-title":"J. Colloid Interface Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1365-2621.2005.00980.x","article-title":"Flavour encapsulation and controlled release\u2014A review","volume":"41","author":"Madene","year":"2006","journal-title":"Int. J. Food Sci. Technol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"559","DOI":"10.3109\/02652048.2015.1065917","article-title":"Formulation and evaluation of Itraconazole nanoemulsion for enhanced oral bioavailability","volume":"32","author":"Thakkar","year":"2015","journal-title":"J. Microencapsul."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.ijpharm.2007.06.035","article-title":"Formulation, antimalarial activity and biodistribution of oral lipid nanoemulsion of primaquine","volume":"347","author":"Singh","year":"2008","journal-title":"Int. J. Pharm."},{"key":"ref_14","first-page":"1","article-title":"Role of nanoemulsion in pharmaceutical sciences-a review","volume":"2","author":"Kumar","year":"2014","journal-title":"AJRPSB"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1007\/s13205-014-0214-0","article-title":"Nanoemulsion: An advanced mode of drug delivery system","volume":"5","author":"Jaiswal","year":"2015","journal-title":"3 Biotech"},{"key":"ref_16","first-page":"393","article-title":"Enhancement of stability and skin permeation by sucrose stearate and cyclodextrins in progesrerone nanoemulsions","volume":"60","author":"Matsko","year":"2010","journal-title":"Int. J. Pharm."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/j.ijpharm.2005.10.002","article-title":"Effect of lipid-containing, positively charged nanoemulsions on skin hydration, elasticity and erythema\u2014An in vivo study","volume":"307","author":"Yilmaz","year":"2006","journal-title":"Int. J. Pharm."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.jab.2017.05.003","article-title":"The role of keratinocytes in inflammation","volume":"15","year":"2017","journal-title":"J. Appl. Biomed."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1016\/j.ejpb.2012.11.027","article-title":"New formulation approaches to improve solubility and drug release from fixed dose combinations: Case examples pioglitazone\/glimepiride and ezetimibe\/simvastatin","volume":"84","author":"Taupitz","year":"2013","journal-title":"Eur. J. Pharm. Biopharm."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Rupa, E.J., Li, J.F., Arif, M.H., Yaxi, H., Puja, A.M., Chan, A.J., Hoang, V., Kaliraj, L., Yang, D.C., and Kang, S.C. (2020). Cordyceps militaris Fungus Extracts-Mediated Nanoemulsion for Improvement Antioxidant, Antimicrobial, and Anti-Inflammatory Activities. Molecules, 25.","DOI":"10.3390\/molecules25235733"},{"key":"ref_21","first-page":"145","article-title":"Nanoemulsions: A new vehicle for skincare products","volume":"108","author":"Simonnet","year":"2004","journal-title":"Adv. Colloid Interface Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1016\/j.ultsonch.2012.08.010","article-title":"Ultrasonic emulsification of food-grade nanoemulsion formulation and evaluation of its bactericidal activity","volume":"20","author":"Ghosh","year":"2013","journal-title":"Ultrason. Sonochemistry"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1123","DOI":"10.1038\/sj.ejcn.1602831","article-title":"Effects of sea buckthorn berries on infections and inflammation: A double-blind, randomized, placebo-controlled trial","volume":"62","author":"Larmo","year":"2008","journal-title":"Eur. J. Clin. Nutr."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.jep.2017.11.022","article-title":"The beneficial health aspects of sea buckthorn (Elaeagnus rhamnoides (L.) A. Nelson) oil","volume":"213","author":"Olas","year":"2018","journal-title":"J. Ethnopharmacol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.foodchem.2015.03.053","article-title":"Sea buckthorn seed oil protects against the oxidative stress produced by thermally oxidized lipids","volume":"186","author":"Zeb","year":"2015","journal-title":"Food Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1477-3155-8-4","article-title":"A potential tocopherol acetate loaded palm oil esters-in-water nanoemulsions for nanocosmeceuticals","volume":"8","author":"Teo","year":"2010","journal-title":"J. Nanobiotechnol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.1080\/01932691.2010.498234","article-title":"Accelerated stability and moisturizing capacity of emulsions presenting lamellar gel phase obtained from brazilian natural raw material","volume":"32","author":"Oliveira","year":"2011","journal-title":"J. Dispers. Sci. Technol."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Li, X., Shen, J., Jiang, Y., Shen, T., You, L., Sun, X., Xu, X., Hu, W., Wu, H., and Wang, G. (2016). Anti-inflammatory effects of chloranthalactone B in LPS-stimulated RAW264. 7 cells. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17111938"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1016\/j.jgr.2018.10.003","article-title":"Compound K, a ginsenoside metabolite, plays an anti-inflammatory role in macrophages by targeting the AKT1-mediated signaling pathway","volume":"43","author":"Lee","year":"2019","journal-title":"J. Ginseng Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1016\/j.ijpharm.2008.07.022","article-title":"Nanoemulsions of an anti-oxidant synergy formulation containing gamma tocopherol have enhanced bioavailability and anti-inflammatory properties","volume":"363","author":"Kuo","year":"2008","journal-title":"Int. J. Pharm."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.colsurfb.2016.04.008","article-title":"Pomegranate seed oil nanoemulsions improve the photostability and in vivo antinociceptive effect of a non-steroidal anti-inflammatory drug","volume":"144","author":"Ferreira","year":"2016","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1016\/j.bmcl.2017.01.061","article-title":"Inhibition of LPS-stimulated ROS production by fluorinated and hydroxylated chalcones in RAW 264.7 macrophages with structure-activity relationship study","volume":"27","author":"Bist","year":"2017","journal-title":"Bioorganic Med. Chem. Lett."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1947","DOI":"10.1016\/j.foodchem.2013.05.021","article-title":"Evaluation of anti-oxidant and anti-cancer properties of Dendropanax morbifera L\u00e9veille","volume":"141","author":"Hyun","year":"2013","journal-title":"Food Chem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.jgr.2015.12.009","article-title":"In vitro antioxidative and anti-inflammatory effects of the compound K-rich fraction BIOGF1K, prepared from Panax ginseng","volume":"41","author":"Hossen","year":"2017","journal-title":"J. Ginseng Res."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/1\/218\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:55:58Z","timestamp":1760169358000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/1\/218"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12,30]]},"references-count":34,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2022,1]]}},"alternative-id":["molecules27010218"],"URL":"https:\/\/doi.org\/10.3390\/molecules27010218","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,12,30]]}}}