{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T13:28:06Z","timestamp":1785850086111,"version":"3.56.0"},"reference-count":67,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"science and technology research of Chongqing Municipal Education Commission","award":["kjzd-k201902802"],"award-info":[{"award-number":["kjzd-k201902802"]}]},{"name":"Education Science Research Program for the 13th Five-year Plan (2018-GX-462),","award":["2018-GX-462"],"award-info":[{"award-number":["2018-GX-462"]}]},{"name":"education and teaching reform in Chongqing Medical and Pharmaceutical College (CQYGZJG1909)","award":["CQYGZJG1909"],"award-info":[{"award-number":["CQYGZJG1909"]}]},{"name":"Talent introduction project of Chongqing Medical and Pharmaceutical College (ygz2016302)","award":["ygz2016302"],"award-info":[{"award-number":["ygz2016302"]}]},{"name":"Scientific Re-search at King Khalid University for funding the work through the research project RGP.2\/156\/42.","award":["RGP.2\/156\/42"],"award-info":[{"award-number":["RGP.2\/156\/42"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Therapeutic effects of anticancer medicines can be improved by targeting the specific receptors on cancer cells. Folate receptor (FR) targeting with antibody (Ab) is an effective tool to deliver anticancer drugs to the cancer cell. In this research project, a novel formulation of targeting drug delivery was designed, and its anticancer effects were analyzed. Folic acid-conjugated magnetic nanoparticles (MNPs) were used for the purification of folate receptors through a novel magnetic affinity purification method. Antibodies against the folate receptors and methotrexate (MTX) were developed and characterized with enzyme-linked immunosorbent assay and Western blot. Targeting nanomedicines (MNP-MTX-FR Ab) were synthesized by engineering the MNP with methotrexate and anti-folate receptor antibody (anti-FR Ab). The cytotoxicity of nanomedicines on HeLa cells was analyzed by calculating the % age cell viability. A fluorescent study was performed with HeLa cells and tumor tissue sections to analyze the binding efficacy and intracellular tracking of synthesized nanomedicines. MNP-MTX-FR Ab demonstrated good cytotoxicity along all the nanocomposites, which confirms that the antibody-coated medicine possesses the potential affinity to destroy cancer cells in the targeted drug delivery process. Immunohistochemical approaches and fluorescent study further confirmed their uptake by FRs on the tumor cells\u2019 surface in antibody-mediated endocytosis. The current approach is a useful addition to targeted drug delivery for better management of cancer therapy along with immunotherapy in the future.<\/jats:p>","DOI":"10.3390\/molecules27010261","type":"journal-article","created":{"date-parts":[[2022,1,7]],"date-time":"2022-01-07T03:46:27Z","timestamp":1641527187000},"page":"261","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["A Novel Method of Magnetic Nanoparticles Functionalized with Anti-Folate Receptor Antibody and Methotrexate for Antibody Mediated Targeted Drug Delivery"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1472-1939","authenticated-orcid":false,"given":"Madeeha Shahzad","family":"Lodhi","sequence":"first","affiliation":[{"name":"School of Biochemistry and Biotechnology, University of the Punjab, Lahore 54890, Pakistan"},{"name":"Institute of molecular Biology and Biotechnology, The University of Lahore, Lahore 58810, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatima","family":"Khalid","sequence":"additional","affiliation":[{"name":"School of Biochemistry and Biotechnology, University of the Punjab, Lahore 54890, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1158-2133","authenticated-orcid":false,"given":"Muhammad Tahir","family":"Khan","sequence":"additional","affiliation":[{"name":"Institute of molecular Biology and Biotechnology, The University of Lahore, Lahore 58810, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zahoor Qadir","family":"Samra","sequence":"additional","affiliation":[{"name":"School of Biochemistry and Biotechnology, University of the Punjab, Lahore 54890, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4908-3313","authenticated-orcid":false,"given":"Shabbir","family":"Muhammad","sequence":"additional","affiliation":[{"name":"Department of Physics, College of Science, King Khalid University, Abha 61413, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6361-0840","authenticated-orcid":false,"given":"Yu-Juan","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Chongqing Normal University, Shapingba, Chongqing 401331, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kejie","family":"Mou","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Bishan Hospital of Chongqing, Chongqing 402760, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,1,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Ferlay, J., Colombet, M., Soerjomataram, I., Parkin, D.M., Pi\u00f1eros, M., Znaor, A., and Bray, F. 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