{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T05:49:36Z","timestamp":1790142576021,"version":"4.0.1"},"reference-count":31,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2022,1,8]],"date-time":"2022-01-08T00:00:00Z","timestamp":1641600000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Key-Area Research and Development Program of Guangdong Province","award":["2020B1111110006"],"award-info":[{"award-number":["2020B1111110006"]}]},{"name":"Special project of Foshan University of science and technology in 2019","award":["FSUST19-SRI10"],"award-info":[{"award-number":["FSUST19-SRI10"]}]},{"name":"GBA Institute of Collaborate Innovation","award":["GICI-022"],"award-info":[{"award-number":["GICI-022"]}]},{"name":"Shenzhen Science and Technology Innovation Committee","award":["ZDSYS201707281432317; JCYJ20170413173747440; JCYJ2018030 6174903174"],"award-info":[{"award-number":["ZDSYS201707281432317; JCYJ20170413173747440; JCYJ2018030 6174903174"]}]},{"DOI":"10.13039\/501100017291","name":"Zhongshan Municipal Bureau of Science and Technology","doi-asserted-by":"publisher","award":["ZSST20SC03"],"award-info":[{"award-number":["ZSST20SC03"]}],"id":[{"id":"10.13039\/501100017291","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Guangzhou Science and Technology Committee Research Grant","award":["GZSTI16SC02; GZSTI17SC02"],"award-info":[{"award-number":["GZSTI16SC02; GZSTI17SC02"]}]},{"name":"Hong Kong RGC Theme-based Research Scheme","award":["T13-605\/18-W"],"award-info":[{"award-number":["T13-605\/18-W"]}]},{"name":"Hong Kong Innovation Technology Fund","award":["UIM\/340, UIM\/385, ITS\/500\/18FP; ITCPD\/17-9; TUYF19SC02, PD18SC01 and HMRF18SC06; HMRF20SC07; AFD20SC01"],"award-info":[{"award-number":["UIM\/340, UIM\/385, ITS\/500\/18FP; ITCPD\/17-9; TUYF19SC02, PD18SC01 and HMRF18SC06; HMRF20SC07; AFD20SC01"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>To search hair growth-promoting herbal extract, a screening platform of having HEK293T fibroblast being transfected with pTOPFLASH DNA construct was developed over a thousand of herbal extracts and phytochemicals were screened. One of the hits was ethanolic extract of Rhizoma Belamcandae, the rhizome of Belamcanda chinensis (L.) DC. Tectoridin, an isoflavone from Rhizoma Belamcandae, was shown to be responsible for this activation of promoter construct, inducing the transcription of pTOPFLASH in the transfected fibroblasts in a dose-dependent manner. The blockage by DKK-1 suggested the action of tectoridin could be mediated by the Wnt receptor. The hair growth-promoting effects of tectoridin were illustrated in human follicular dermal papilla cells and mouse vibrissae organ cultures. In tectoridin-treated dermal papilla cultures, an activation of Wnt signaling was demonstrated by various indicative markers, including TCF\/LEF1 transcriptional activity, nuclear translocation of \u03b2-catenin, expressions level of mRNAs encoding axin-related protein, (AXIN2), \u03b2-catenin, lymphoid enhancer-binding factor-1 (LEF-1), insulin-like growth factor 1 (IGF-1) and alkaline phosphatase (ALP). In addition, an increase of hair shaft elongation was observed in cultured mouse vibrissae upon the treatment of tectoridin. Tectoridin, as well as the herbal extract of Rhizoma Belamcandae, possesses hair promoting activity, which deserves further development.<\/jats:p>","DOI":"10.3390\/molecules27020400","type":"journal-article","created":{"date-parts":[[2022,1,9]],"date-time":"2022-01-09T23:06:15Z","timestamp":1641769575000},"page":"400","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Tectoridin Stimulates the Activity of Human Dermal Papilla Cells and Promotes Hair Shaft Elongation in Mouse Vibrissae Hair Follicle Culture"],"prefix":"10.3390","volume":"27","author":[{"given":"Gary Ka-Wing","family":"Yuen","sequence":"first","affiliation":[{"name":"Shenzhen Key Laboratory of Edible and Medicinal Bioresources, HKUST Shenzhen Research Institute, Shenzhen 518057, China"},{"name":"Division of Life Science and Centre for Chinese Medicine, The Hong Kong University of Science and Technology, Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bryan Siu-Yin","family":"Ho","sequence":"additional","affiliation":[{"name":"Division of Life Science and Centre for Chinese Medicine, The Hong Kong University of Science and Technology, Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lish Sheng-Ying","family":"Lin","sequence":"additional","affiliation":[{"name":"Division of Life Science and Centre for Chinese Medicine, The Hong Kong University of Science and Technology, Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tina Ting-Xia","family":"Dong","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory of Edible and Medicinal Bioresources, HKUST Shenzhen Research Institute, Shenzhen 518057, China"},{"name":"Division of Life Science and Centre for Chinese Medicine, The Hong Kong University of Science and Technology, Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karl Wah-Keung","family":"Tsim","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory of Edible and Medicinal Bioresources, HKUST Shenzhen Research Institute, Shenzhen 518057, China"},{"name":"Division of Life Science and Centre for Chinese Medicine, The Hong Kong University of Science and Technology, Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,1,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/S1471-4914(01)02027-5","article-title":"Towards a molecular understanding of hair loss and its treatment","volume":"7","author":"Cotsarelis","year":"2001","journal-title":"Trends Mol. 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