{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T01:22:07Z","timestamp":1775784127713,"version":"3.50.1"},"reference-count":35,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2022,1,18]],"date-time":"2022-01-18T00:00:00Z","timestamp":1642464000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The National Natural Science Foundation","award":["No.81971514, 81803202"],"award-info":[{"award-number":["No.81971514, 81803202"]}]},{"DOI":"10.13039\/501100012245","name":"Science and Technology Planning Project of Guangdong Province","doi-asserted-by":"publisher","award":["2020A1515010029"],"award-info":[{"award-number":["2020A1515010029"]}],"id":[{"id":"10.13039\/501100012245","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Science and Technology Program of Shenzhen","award":["KQTD20170331145453160, JCYJ20180306171550045, GJHZ20190822095605512"],"award-info":[{"award-number":["KQTD20170331145453160, JCYJ20180306171550045, GJHZ20190822095605512"]}]},{"name":"Science and Technology Program of Nanshan","award":["LHTD20180007"],"award-info":[{"award-number":["LHTD20180007"]}]},{"DOI":"10.13039\/100013262","name":"State Key Laboratory of Respiratory Disease","doi-asserted-by":"publisher","award":["SKLRD-Z-202111, SKLRD-OP-201910, SKLRD-Z-202216"],"award-info":[{"award-number":["SKLRD-Z-202111, SKLRD-OP-201910, SKLRD-Z-202216"]}],"id":[{"id":"10.13039\/100013262","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Key Science and Technology Plan Project of Zigong City","award":["2019YYJC26"],"award-info":[{"award-number":["2019YYJC26"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Ambrosia artemisiifolia (Amb a) contains many allergens. Allergic conjunctivitis caused by Ambrosia artemisiifolia and its related allergen-specific immunotherapy (AIT) are seldom studied at present. poly(DL-lactide-co-glycolide)-polyethylene glycol (PLGA-PEG) is a very good nano-carrier, which has been applied in the medical field. In this context, we studied the immunotherapy effect and potential mechanism of recombinant Amb a 1 (rAmb a 1)-loaded PLGA-PEG nanoparticles. A mouse allergic conjunctivitis model was established with Ambrosia artemisiifolia crude extract, and the nanoparticles were used for AIT through direct observation of conjunctival tissue, degranulation of mast cells in conjunctival tissue, serum-specific antibodies, cytokines and other assessment models. The treatment of nanoparticles enhanced the secretion of T-helper 1 (Th1) cytokine Interferon-gama (IFN-\u03b3) and the production of immunoglobulin G (IgG)2a (IgG2a), inhibited the secretion of T-helper 2 (Th2) cytokine Interleukin (IL)-13 and IL-4 and the level of IgE. Especially, degranulation of mast cells and expression of mast cell protease-1 (MCP-1) in conjunctival tissue was reduced significantly. In this study, we proved that the nanoparticles prepared by rAmb a 1 and PLGA-PEG have an immunotherapy effect on allergic conjunctivitis in mice.<\/jats:p>","DOI":"10.3390\/molecules27030598","type":"journal-article","created":{"date-parts":[[2022,1,18]],"date-time":"2022-01-18T22:46:32Z","timestamp":1642545992000},"page":"598","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Effects of rAmb a 1-Loaded PLGA-PEG Nanoparticles in a Murine Model of Allergic Conjunctivitis"],"prefix":"10.3390","volume":"27","author":[{"given":"Hui","family":"Cao","sequence":"first","affiliation":[{"name":"State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen Key Laboratory of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen 518061, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen Key Laboratory of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen 518061, China"},{"name":"Sichuan Vocational College of Health and Rehabilitation, Zigong 643000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junyi","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen Key Laboratory of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen 518061, China"},{"name":"Laboratory of Allergy and Precision Medicine, Chengdu Institute of Respiratory Health, The Third People\u2019s Hospital of Chengdu, Chengdu 610031, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miao","family":"Gong","sequence":"additional","affiliation":[{"name":"Department of Respirology & Allergy, Third Affiliated Hospital of Shenzhen University, Shenzhen 518020, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruyi","family":"Yuan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen Key Laboratory of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen 518061, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiahua","family":"Lu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen Key Laboratory of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen 518061, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaojun","family":"Xiao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen Key Laboratory of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen 518061, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyu","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen Key Laboratory of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen 518061, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,1,18]]},"reference":[{"key":"ref_1","unstructured":"Baab, S., Le, P.H., and Kinzer, E.E. 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