{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T04:24:38Z","timestamp":1783571078756,"version":"3.55.0"},"reference-count":50,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2022,1,18]],"date-time":"2022-01-18T00:00:00Z","timestamp":1642464000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Ampelopsis grossedentata (AG) is an ancient medicinal plant that is mainly distributed and used in southwest China. It exerts therapeutic effects, such as antioxidant, anti-diabetic, and anti-inflammatory activities, reductions in blood pressure and cholesterol and hepatoprotective effects. Researchers in China recently reported the anti-obesity effects of AG extract in diet-induced obese mice and rats. To verify these findings, we herein investigated the effects of AG extract and its principal compound, ampelopsin, in high-fat diet (HFD)- and alcohol diet-fed mice, olive oil-loaded mice, and differentiated 3T3-L1 cells. The results obtained showed that AG extract and ampelopsin significantly suppressed increases in the weights of body, livers and abdominal fat and also up-regulated the expression of carnitine palmitoyltransferase 1A in HFD-fed mice. In olive oil-loaded mice, AG extract and ampelopsin significantly attenuated increases in serum triglyceride (TG) levels. In differentiated 3T3-L1 cells, AG extract and ampelopsin promoted TG decomposition, which appeared to be attributed to the expression of hormone-sensitive lipase. In alcohol diet-fed mice, AG extract and ampelopsin reduced serum levels of ethanol, glutamic oxaloacetic transaminase (GOT), and glutamic pyruvic transaminase (GPT) and liver TG. An examination of metabolic enzyme expression patterns revealed that AG extract and ampelopsin mainly enhanced the expression of aldehyde dehydrogenase and suppressed that of cytochrome P450, family 2, subfamily e1. In conclusion, AG extract and ampelopsin suppressed diet-induced intestinal fat accumulation and reduced the risk of fatty liver associated with HFD and alcohol consumption.<\/jats:p>","DOI":"10.3390\/molecules27030607","type":"journal-article","created":{"date-parts":[[2022,1,18]],"date-time":"2022-01-18T22:46:32Z","timestamp":1642545992000},"page":"607","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["The Anti-Adiposity Mechanisms of Ampelopsin and Vine Tea Extract in High Fat Diet and Alcohol-Induced Fatty Liver Mouse Models"],"prefix":"10.3390","volume":"27","author":[{"given":"Jianbo","family":"Wu","sequence":"first","affiliation":[{"name":"Research and Development Division, Oryza Oil and Fat Chemical Co., Ltd., 1 Numata, Kitagata-cho, Ichinomiya 493-8001, Aichi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenchi","family":"Miyasaka","sequence":"additional","affiliation":[{"name":"Research and Development Division, Oryza Oil and Fat Chemical Co., Ltd., 1 Numata, Kitagata-cho, Ichinomiya 493-8001, Aichi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wakana","family":"Yamada","sequence":"additional","affiliation":[{"name":"Research and Development Division, Oryza Oil and Fat Chemical Co., Ltd., 1 Numata, Kitagata-cho, Ichinomiya 493-8001, Aichi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shogo","family":"Takeda","sequence":"additional","affiliation":[{"name":"Research and Development Division, Oryza Oil and Fat Chemical Co., Ltd., 1 Numata, Kitagata-cho, Ichinomiya 493-8001, Aichi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norihito","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Research and Development Division, Oryza Oil and Fat Chemical Co., Ltd., 1 Numata, Kitagata-cho, Ichinomiya 493-8001, Aichi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Shimoda","sequence":"additional","affiliation":[{"name":"Research and Development Division, Oryza Oil and Fat Chemical Co., Ltd., 1 Numata, Kitagata-cho, Ichinomiya 493-8001, Aichi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,1,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"154388","DOI":"10.1016\/j.metabol.2020.154388","article-title":"Factors affecting weight loss variability in obesity","volume":"113","author":"Dent","year":"2020","journal-title":"Metabolism"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.metabol.2018.11.014","article-title":"Obesity and nonalcoholic fatty liver disease: From pathophysiology to therapeutics","volume":"92","author":"Polyzos","year":"2018","journal-title":"Metabolism"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1016\/j.gtc.2016.07.011","article-title":"Pharmacotherapy in Treatment of Obesity","volume":"45","author":"Keith","year":"2016","journal-title":"Gastroenterol. Clin. N. Am."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Shin, S.S., and Yoon, M. (2020). Regulation of Obesity by Antiangiogenic Herbal Medicines. Molecules, 25.","DOI":"10.3390\/molecules25194549"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"802","DOI":"10.4093\/dmj.2020.0258","article-title":"Comprehensive Review of Current and Upcoming Anti-Obesity Drugs","volume":"44","author":"Son","year":"2020","journal-title":"Diabetes Metab. J."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"941","DOI":"10.2165\/00003088-200342110-00002","article-title":"Pharmacokinetics of L-Carnitine","volume":"42","author":"Evans","year":"2003","journal-title":"Clin. Pharmacokinet."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1080\/07315724.2006.10719518","article-title":"Beneficial Effects of Green Tea\u2014A Review","volume":"25","author":"Cabrera","year":"2006","journal-title":"J. Am. Coll. Nutr."},{"key":"ref_8","first-page":"2","article-title":"Research progress on active components and efficacy of vine tea","volume":"3","author":"Zhou","year":"2014","journal-title":"Tea Fujian"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1016\/j.jpha.2020.10.002","article-title":"Recent advances in research on vine tea, a potential and functional herbal tea with dihydromyricetin and myricetin as major bioactive compounds","volume":"11","author":"Zhang","year":"2020","journal-title":"J. Pharm. Anal."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Wan, W., Jiang, B., Sun, L., Xu, L., and Xiao, P. (2017). Metabolomics reveals that vine tea (Ampelopsis grossedentata) prevents high-fat-diet-induced metabolism disorder by improving glucose homeostasis in rats. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0182830"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"5976","DOI":"10.1039\/D0FO00795A","article-title":"Ameliorative effects and molecular mechanisms of vine tea on western diet-induced NAFLD","volume":"11","author":"Xie","year":"2020","journal-title":"Food Funct."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.phrs.2015.05.009","article-title":"Dihy-dromyricetin improves glucose and lipid metabolism and exerts anti-inflammatory effects in nonalcoholic fatty liver disease: A randomized controlled trial","volume":"99","author":"Chen","year":"2015","journal-title":"Pharmacol. Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1046","DOI":"10.1111\/acer.14326","article-title":"Dihydromyricetin Protects the Liver via Changes in Lipid Metabolism and Enhanced Ethanol Metabolism","volume":"44","author":"Silva","year":"2020","journal-title":"Alcohol. Clin. Exp. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/B978-0-12-800213-1.00035-3","article-title":"Chapter 35. Ethanol Metabolism and Implications for Disease","volume":"Volume 1","author":"Rajendram","year":"2016","journal-title":"Neuropathology of Drug Addictions and Substance Misuse"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"3161","DOI":"10.18632\/aging.101623","article-title":"AMPK\u03b1 pathway involved in hepatic triglyceride metabolism disorder in diet-induced obesity mice following Escherichia coli Infection","volume":"10","author":"Fang","year":"2018","journal-title":"Aging"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"67","DOI":"10.59566\/IJBS.2015.11067","article-title":"Purple Tea and Its Extract Suppress Diet-induced Fat Accumulation in Mice and Human Subjects by Inhibiting Fat Absorption and Enhancing Hepatic Carnitine Palmitoyltransferase Expression","volume":"11","author":"Shimoda","year":"2015","journal-title":"Int. J. Biomed. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"103741","DOI":"10.1016\/j.jff.2019.103741","article-title":"Effect of Spirulina lipids on high-fat and high-sucrose diet induced obesity and hepatic lipid accumulation in C57BL\/6J mice","volume":"65","author":"Yang","year":"2020","journal-title":"J. Funct. Foods"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"113927","DOI":"10.1016\/j.bcp.2020.113927","article-title":"Modulation of SIRT1-mediated signaling cascades in the liver contributes to the amelioration of nonalcoholic steatohepatitis in high fat fed middle-aged LDL receptor knockout mice by dihydromyricetin","volume":"175","author":"Zeng","year":"2020","journal-title":"Biochem. Pharmacol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"594574","DOI":"10.3389\/fphar.2020.594574","article-title":"Bilirubin Nanoparticles Reduce Diet-Induced Hepatic Steatosis, Improve Fat Utilization, and Increase Plasma \u03b2-Hydroxybutyrate","volume":"11","author":"Hinds","year":"2020","journal-title":"Front. Pharmacol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/0092-8674(74)90126-3","article-title":"Sublines of mouse 3T3 cells that accumulate lipid","volume":"1","author":"Green","year":"1974","journal-title":"Cell"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1258\/ebm.2010.010063","article-title":"Cell line models for differentiation: Preadipocytes and adipocytes","volume":"235","author":"Poulos","year":"2010","journal-title":"Exp. Biol. Med."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1247","DOI":"10.1111\/jcmm.13403","article-title":"Dihydromyricetin enhances glucose uptake by inhibition of MEK\/ERK pathway and consequent down-regulation of phosphorylation of PPAR\u03b3 in 3T3-L1 cells","volume":"22","author":"Liu","year":"2018","journal-title":"J. Cell. Mol. Med."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1124\/pr.58.4.5","article-title":"International Union of Pharmacology. LXI. Peroxisome proliferator-activated receptors","volume":"58","author":"Michalik","year":"2006","journal-title":"Pharmacol. Rev."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Dunning, K.R., Anastasi, M.R., Zhang, V.J., Russell, D.L., and Robker, R.L. (2014). Regulation of Fatty Acid Oxidation in Mouse Cumulus-Oocyte Complexes during Maturation and Modulation by PPAR Agonists. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0087327"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1146\/annurev.med.53.082901.104018","article-title":"The Mechanisms of Action of PPARs","volume":"53","author":"Berger","year":"2002","journal-title":"Annu. Rev. Med."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Wagner, N., and Wagner, K.-D. (2020). The Role of PPARs in Disease. Cells, 9.","DOI":"10.3390\/cells9112367"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"759","DOI":"10.33549\/physiolres.934411","article-title":"Peroxisome proliferator-activated receptor \u03b3 in white and brown adipocyte regulation and differentiation","volume":"69","author":"Wu","year":"2020","journal-title":"Physiol. Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1016\/0092-8674(94)90006-X","article-title":"Stimulation of adipogenesis in fibroblasts by PPAR\u03b32, a lipid-activated transcription factor","volume":"79","author":"Tontonoz","year":"1994","journal-title":"Cell"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"eabb5280","DOI":"10.1126\/scitranslmed.abb5280","article-title":"Diet-induced obesity promotes infection by impairment of the innate antimicrobial defense function of dermal adipocyte progenitors","volume":"13","author":"Zhang","year":"2021","journal-title":"Sci. Transl. Med."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"111109","DOI":"10.1016\/j.mce.2020.111109","article-title":"Adipocyte-specific deletion of zinc finger protein 407 results in lipodystrophy and insulin resistance in mice","volume":"521","author":"Charrier","year":"2020","journal-title":"Mol. Cell. Endocrinol."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Zhang, S., Yan, L., and Kim, S.M. (2020). Vanadium-protein complex inhibits human adipocyte differentiation through the activation of \u03b2-catenin and LKB1\/AMPK signaling pathway. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0239547"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-020-63579-3","article-title":"Hepatocyte-specific deletion of Ppar\u03b1 promotes NAFLD in the context of obesity","volume":"10","author":"Regnier","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1202","DOI":"10.1136\/gutjnl-2015-310798","article-title":"Liver PPAR\u03b1 is crucial for whole-body fatty acid homeostasis and is protective against NAFLD","volume":"65","author":"Montagner","year":"2016","journal-title":"Gut"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"9804","DOI":"10.1074\/jbc.RA120.013700","article-title":"Bilirubin remodels murine white adipose tissue by reshaping mito-chondrial activity and the coregulator profile of peroxisome proliferator\u2013activated receptor \u03b1","volume":"29","author":"Gordon","year":"2020","journal-title":"J. Biol. Chem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1007\/s13679-014-0129-4","article-title":"Alcohol Consumption and Obesity: An Update","volume":"4","author":"Traversy","year":"2015","journal-title":"Curr. Obes. Rep."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1111\/j.1753-4887.2012.00548.x","article-title":"Is beer consumption related to measures of abdominal and general obesity? A systematic review and meta-analysis","volume":"71","author":"Bendsen","year":"2012","journal-title":"Nutr. Rev."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1111\/j.1753-4887.2011.00403.x","article-title":"Alcohol consumption and body weight: A systematic review","volume":"69","year":"2011","journal-title":"Nutr. Rev."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.physbeh.2010.01.012","article-title":"Alcohol, appetite and energy balance: Is alcohol intake a risk factor for obesity?","volume":"100","author":"Yeomans","year":"2010","journal-title":"Physiol. Behav."},{"key":"ref_39","unstructured":"National Institute on Alcohol Abuse and Alcoholism (2007, April 01). Alcohol Metabolism: An Update, Available online: https:\/\/pubs.niaaa.nih.gov\/publications\/aa72\/aa72.htm."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"6926","DOI":"10.3748\/wjg.v12.i43.6926","article-title":"Role of ethanol in the regulation of hepatic stellate cell Function","volume":"12","author":"Wang","year":"2006","journal-title":"World J. Gastroenterol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/j.jchromb.2007.07.049","article-title":"Simultaneous determination and pharmacokinetic studies of dihy-dromyricetin and myricetin in rat plasma by HPLC-DAD after oral administration of Ampelopsis grossedentata decoction","volume":"860","author":"Zhang","year":"2007","journal-title":"J. Chromatogr. B"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1039\/a909219f","article-title":"HPLC determination of catechins and caffeine in tea. Differentiation of green, black and instant teas","volume":"125","author":"Pablos","year":"2000","journal-title":"Analyst"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1786","DOI":"10.1021\/jf803441c","article-title":"Effect of Polyphenol-Rich Extract from Walnut on Diet-Induced Hypertriglyceridemia in Mice via Enhancement of Fatty Acid Oxidation in the Liver","volume":"57","author":"Shimoda","year":"2009","journal-title":"J. Agric. Food Chem."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Shimoda, H., Seki, E., and Aitani, M. (2006). Inhibitory effect of green coffee bean extract on fat accumulation and body weight gain in mice. BMC Complement. Altern. Med., 6.","DOI":"10.1186\/1472-6882-6-9"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"780","DOI":"10.1248\/bpb.22.780","article-title":"A vanadyl sulfate-bovine serum albumin complex stimulates the release of lipoprotein lipase activity from isolated rat fat pads through an increase in the cellular content of cAMP and myo-inositol 1, 4, 5-trisphosphate","volume":"22","author":"Motoyashiki","year":"1999","journal-title":"Biol. Pharm. Bull."},{"key":"ref_46","first-page":"335","article-title":"Fatty Acid Release Rate of Adipose Tissue from Rats on a High Carbohydrate or a High Fat Diet and its Response to PTU Administration","volume":"34","author":"Suzuki","year":"1981","journal-title":"J. Jpn. Soc. Food Nutr."},{"key":"ref_47","first-page":"e55625","article-title":"Measurement of Basal and Forskolin-stimulated Lipolysis in Inguinal Adipose Fat Pads","volume":"125","author":"Baskaran","year":"2017","journal-title":"J. Vis. Exp."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1819","DOI":"10.1093\/jn\/132.7.1819","article-title":"Salacia reticulata and Its Polyphenolic Constituents with Lipase Inhibitory and Lipolytic Activities Have Mild Antiobesity Effects in Rats","volume":"132","author":"Yoshikawa","year":"2002","journal-title":"J. Nutr."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1943","DOI":"10.1016\/j.bmcl.2004.01.091","article-title":"Carnosic acid, a new class of lipid absorption inhibitor from sage","volume":"14","author":"Ninomiya","year":"2004","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1038\/nprot.2013.032","article-title":"Mouse model of chronic and binge ethanol feeding (the NIAAA model)","volume":"8","author":"Bertola","year":"2013","journal-title":"Nat. Protoc."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/3\/607\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:03:37Z","timestamp":1760133817000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/3\/607"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1,18]]},"references-count":50,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2022,2]]}},"alternative-id":["molecules27030607"],"URL":"https:\/\/doi.org\/10.3390\/molecules27030607","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1,18]]}}}