{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T23:50:36Z","timestamp":1791589836745,"version":"4.3.4"},"reference-count":40,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2022,1,20]],"date-time":"2022-01-20T00:00:00Z","timestamp":1642636800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>A number of studies have confirmed the relationship between constipation and gut microbiota. Additionally, many human and animal experiments have identified probiotics as effectors for the relief of constipation symptoms. In this study, probiotic compounds, including Lactobacillus acidophilus LA11-Onlly, Lacticaseibacillus rhamnosus LR22, Limosilactobacillus reuteri LE16, Lactiplantibacillus plantarum LP-Onlly, and Bifidobacterium animalis subsp. lactis BI516, were administered to mice with loperamide-induced constipation, and the impacts of these strains on constipation-related indicators and gut microbiota were evaluated. The effects of probiotic compounds on constipation relief were associated with various aspects, including gastrointestinal transit rate, number and weight of stools, serum and intestinal gastrointestinal regulatory hormones, and serum cytokines. Some of the probiotic compounds, including Limosilactobacillus reuteri, Lactiplantibacillus plantarum, and Lacticaseibacillus rhamnosus, were found to colonize the intestinal tract. Furthermore, higher dosages promoted the colonization of specific strains. This study yields a new perspective for the clinical use of probiotics to improve constipation symptoms by combining strains with different mechanisms for the alleviation of constipation.<\/jats:p>","DOI":"10.3390\/molecules27030666","type":"journal-article","created":{"date-parts":[[2022,1,20]],"date-time":"2022-01-20T22:51:06Z","timestamp":1642719066000},"page":"666","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["Efficacy of Probiotic Compounds in Relieving Constipation and Their Colonization in Gut Microbiota"],"prefix":"10.3390","volume":"27","author":[{"given":"Yuan","family":"He","sequence":"first","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China"},{"name":"School of Food Science and Technology, Jiangnan University, Wuxi 214122, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leilei","family":"Zhu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jialun","family":"Chen","sequence":"additional","affiliation":[{"name":"Sirio Pharma Co., Ltd., Shantou 515000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Tang","sequence":"additional","affiliation":[{"name":"Sirio Pharma Co., Ltd., Shantou 515000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingluo","family":"Pan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiwei","family":"Yuan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China"},{"name":"School of Food Science and Technology, Jiangnan University, Wuxi 214122, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7148-7555","authenticated-orcid":false,"given":"Hongchao","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China"},{"name":"School of Food Science and Technology, Jiangnan University, Wuxi 214122, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,1,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1111\/apt.15073","article-title":"Randomised clinical trial: Bifidobacterium lactis NCC2818 probiotic vs placebo, and impact on gut transit time, symptoms, and gut microbiology in chronic constipation","volume":"49","author":"Dimidi","year":"2019","journal-title":"Aliment. Pharmacol. Ther."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1038\/nrgastro.2016.53","article-title":"Diagnosis and management of chronic constipation in adults","volume":"13","author":"Rao","year":"2016","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"ref_3","first-page":"646","article-title":"Effect of Descurainia sophia (L.) Webb ex Prantl on Adult Functional Constipation: A Prospective Pilot Study","volume":"22","author":"Choopani","year":"2017","journal-title":"J. Evid. Based Integr. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1007\/164_2016_111","article-title":"Constipation: Pathophysiology and Current Therapeutic Approaches","volume":"239","author":"Sharma","year":"2017","journal-title":"Handb. Exp. Pharmacol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1111\/j.1365-2036.2006.03238.x","article-title":"Chronic constipation: A survey of the patient perspective","volume":"25","author":"Johanson","year":"2007","journal-title":"Aliment. Pharmacol. Ther."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1007\/s11938-017-0118-2","article-title":"Update on the Management of Chronic Constipation","volume":"15","author":"Lacy","year":"2017","journal-title":"Curr. Treat. Options Gastroenterol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.ijsu.2020.04.063","article-title":"The efficacy and safety of probiotics for patients with constipation-predominant irritable bowel syndrome: A systematic review and meta-analysis based on seventeen randomized controlled trials","volume":"79","author":"Wen","year":"2020","journal-title":"Int. J. Surg."},{"key":"ref_8","first-page":"103","article-title":"Are probiotics useful in the treatment of chronic idiopathic constipation in adults? A review of existing systematic reviews, meta-analyses, and recommendations","volume":"15","author":"Koulaouzidis","year":"2020","journal-title":"Prz. Gastroenterol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1017\/S0029665119000934","article-title":"Probiotics and constipation: Mechanisms of action, evidence for effectiveness and utilisation by patients and healthcare professionals","volume":"79","author":"Dimidi","year":"2020","journal-title":"Proc. Nutr. Soc."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"137","DOI":"10.3920\/BM2018.0050","article-title":"Effects of long-term administration of Lactobacillus reuteri DSM-17938 on circulating levels of 5-HT and BDNF in adults with functional constipation","volume":"10","author":"Riezzo","year":"2019","journal-title":"Benef. Mirbobes"},{"key":"ref_11","first-page":"629","article-title":"Effects of probiotic-containing products on stool frequency and intestinal transit in constipated adults: Systematic review and meta-analysis of randomized controlled trials","volume":"30","author":"Miller","year":"2017","journal-title":"Ann. Gastroenterol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1016\/j.chom.2018.05.004","article-title":"Gut Microbiota-Produced Tryptamine Activates an Epithelial G-Protein-Coupled Receptor to Increase Colonic Secretion","volume":"23","author":"Bhattarai","year":"2018","journal-title":"Cell Host Microbe"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1038\/s41587-019-0209-9","article-title":"Reproducible, interactive, scalable, and extensible microbiome data science using QIIME 2","volume":"37","author":"Bolyen","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Hu, L.J., Lu, W.W., Wang, L.L., Pan, M.L., Zhang, H., Zhao, J.X., and Chen, W. (2017). Assessment of Bifidobacterium Species Using groEL Gene on the Basis of Illumina MiSeq High-Throughput Sequencing. Genes, 8.","DOI":"10.3390\/genes8110336"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Xie, M.Q., Pan, M.L., Jiang, Y., Liu, X.M., Lu, W.W., Zhao, J.X., Zhang, H., and Chen, W. (2019). groEL Gene-Based Phylogenetic Analysis of Lactobacillus Species by High-Throughput Sequencing. Genes, 10.","DOI":"10.3390\/genes10070530"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.jaci.2012.10.026","article-title":"A microbiota signature associated with experimental food allergy promotes allergic sensitization and anaphylaxis","volume":"131","author":"Rivas","year":"2013","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1186\/gb-2011-12-6-r60","article-title":"Metagenomic biomarker discovery and explanation","volume":"12","author":"Segata","year":"2011","journal-title":"Genome Biol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1038\/s41575-019-0222-y","article-title":"Management of functional constipation in children and adults","volume":"17","author":"Vriesman","year":"2020","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1007\/s11894-015-0471-z","article-title":"Chronic Constipation: A Review of Current Literature","volume":"17","author":"Sbahi","year":"2015","journal-title":"Curr. Gastroenterol. Rep."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1016\/j.jpeds.2014.09.067","article-title":"Pharmacologic Treatment in Pediatric Functional Abdominal Pain Disorders: A Systematic Review","volume":"166","author":"Korterink","year":"2015","journal-title":"J. Pediatr."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"3587","DOI":"10.1039\/C6FO01641C","article-title":"Bifidobacteria exert species-specific effects on constipation in BALB\/c mice","volume":"8","author":"Wang","year":"2017","journal-title":"Food Funct."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"e772","DOI":"10.1212\/WNL.0000000000010998","article-title":"Probiotics for Constipation in Parkinson Disease: A Randomized Placebo-Controlled Study","volume":"96","author":"Tan","year":"2021","journal-title":"Neurology"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"423","DOI":"10.3389\/fcimb.2020.00423","article-title":"Lactobacillus rhamnosus Strains Relieve Loperamide-Induced Constipation via Different Pathways Independent of Short-Chain Fatty Acids","volume":"10","author":"Wang","year":"2020","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2754","DOI":"10.1007\/s10620-018-5139-8","article-title":"Probiotics Ameliorate Stool Consistency in Patients with Chronic Constipation: A Randomized, Double-Blind, Placebo-Controlled Study","volume":"63","author":"Yoon","year":"2018","journal-title":"Dig. Dis. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1080\/19490976.2017.1412908","article-title":"Effects of 28-day Bifidobacterium animalis subsp lactis HN019 supplementation on colonic transit time and gastrointestinal symptoms in adults with functional constipation: A double-blind, randomized, placebo-controlled, and dose-ranging trial","volume":"9","author":"Ibarra","year":"2018","journal-title":"Gut Microbes"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1274","DOI":"10.1212\/WNL.0000000000003127","article-title":"Probiotics and prebiotic fiber for constipation associated with Parkinson disease: An RCT","volume":"87","author":"Barichella","year":"2016","journal-title":"Neurology"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1016\/j.cgh.2018.08.054","article-title":"The Gut Microbiome in Adult and Pediatric Functional Gastrointestinal Disorders","volume":"17","author":"Shin","year":"2019","journal-title":"Clin. Gastroenterol. Hepatol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1152\/physiolgenomics.00082.2014","article-title":"Structural changes in the gut microbiome of constipated patients","volume":"46","author":"Zhu","year":"2014","journal-title":"Physiol. Genom."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"G368","DOI":"10.1152\/ajpgi.2001.280.3.G368","article-title":"Influence of microbial species on small intestinal myoelectric activity and transit in germ-free rats","volume":"280","author":"Husebye","year":"2001","journal-title":"Am. J. Physiol. Gastroint. Liver Physiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1016\/j.jbiosc.2011.12.016","article-title":"Effects of administration of Bifidobacterium animalis subsp lactis GCL2505 on defecation frequency and bifidobacterial microbiota composition in humans","volume":"113","author":"Ishizuka","year":"2012","journal-title":"J. Biosci. Bioeng."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1120","DOI":"10.1038\/pr.2018.34","article-title":"Bifidobacterium longum subsp infantis CECT7210-supplemented formula reduces diarrhea in healthy infants: A randomized controlled trial","volume":"83","author":"Escribano","year":"2018","journal-title":"Pediatr. Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1111\/1751-2980.12797","article-title":"Impact of a probiotic product on bowel habits and microbial profile in participants with functional constipation: A randomized controlled trial","volume":"20","author":"Martoni","year":"2019","journal-title":"J. Dig. Dis."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1173","DOI":"10.1016\/j.foodres.2018.09.062","article-title":"Laxative effect of probiotic chocolate on loperamide-induced constipation in rats","volume":"116","author":"Eor","year":"2019","journal-title":"Food Res. Int."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.carbpol.2020.117218","article-title":"Chitosan oligosaccharides attenuate loperamide-induced constipation through regulation of gut microbiota in mice","volume":"253","author":"Zhang","year":"2021","journal-title":"Carbohydr. Polym."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1053\/j.gastro.2013.01.047","article-title":"Complex Interactions Among Diet, Gastrointestinal Transit, and Gut Microbiota in Humanized Mice","volume":"144","author":"Kashyap","year":"2013","journal-title":"Gastroenterology"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1053\/j.gastro.2012.09.055","article-title":"The Receptor TGR5 Mediates the Prokinetic Actions of Intestinal Bile Acids and Is Required for Normal Defecation in Mice","volume":"144","author":"Alemi","year":"2013","journal-title":"Gastroenterology"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1968","DOI":"10.1136\/gutjnl-2016-313271","article-title":"Prebiotic inulin-type fructans induce specific changes in the human gut microbiota","volume":"66","author":"Vandeputte","year":"2017","journal-title":"Gut"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2960","DOI":"10.1016\/j.clnu.2020.01.005","article-title":"Meta-analysis of randomized controlled trials of the effects of probiotics on functional constipation in adults","volume":"39","author":"Zhang","year":"2020","journal-title":"Clin. Nutr."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Liu, A.L.T., Chen, S., Jena, P.K., Sheng, L.L., Hu, Y., and Wan, Y.J.Y. (2021). Probiotics Improve Gastrointestinal Function and Life Quality in Pregnancy. Nutrients, 13.","DOI":"10.3390\/nu13113931"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"3917","DOI":"10.1007\/s00018-019-03190-6","article-title":"Bridging intestinal immunity and gut microbiota by metabolites","volume":"76","author":"Wang","year":"2019","journal-title":"Cell. Mol. Life Sci."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/3\/666\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:04:37Z","timestamp":1760133877000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/3\/666"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1,20]]},"references-count":40,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2022,2]]}},"alternative-id":["molecules27030666"],"URL":"https:\/\/doi.org\/10.3390\/molecules27030666","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1,20]]}}}