{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T19:10:01Z","timestamp":1773429001641,"version":"3.50.1"},"reference-count":52,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2022,1,25]],"date-time":"2022-01-25T00:00:00Z","timestamp":1643068800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fagron do Brasil Pharmaceutical LTDA.","award":["N\/A"],"award-info":[{"award-number":["N\/A"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Endometriosis presents high prevalence and its physiopathology involves hyperactivation of endometrial and vaginal cells, especially by bacteria. The disease has no cure and therapies aiming to inhibit its development are highly desirable. Therefore, this study investigated whether MiodesinTM (10 \u00b5g\/mL = IC80; 200 \u00b5g\/mL = IC50), a natural compound constituted by Uncaria tomentosa, Endopleura uchi, and astaxanthin, could exert anti-inflammatory and anti-proliferative effects against Lipopolysaccharides (LPS) stimulation in endometrial and Candida albicans vaginal cell lines. VK2 E6\/E7 (vaginal) and KLE (epithelial) cell lines were stimulated with Candida albicans (1 \u00d7 107 to 5 \u00d7 107\/mL) and LPS (1 \u03bcg\/mL), respectively. MiodesinTM inhibited mRNA expression for Nuclear factor kappa B (NF-\u03baB), ciclo-oxigenase 1 (COX-1), and phospholipase A2 (PLA2), beyond the C\u2013C motif chemokine ligand 2 (CCL2), CCL3, and CCL5 in VK2 E6\/E7 cells (p &lt; 0.05). In addition, the inhibitory effects of both doses of MiodesinTM (10 \u00b5g\/mL and 200 \u00b5g\/mL) resulted in reduced secretion of interleukin-1\u03b2 (IL-1\u03b2), IL-6, IL-8, tumor necrosis factor \u03b1 (TNF-\u03b1) (24 h, 48 h, and 72 h) and CCL2, CCL3, and CLL5 (p &lt; 0.05) by VK2 E6\/E7 cells. In the same way, COX-1 MiodesinTM inhibited LPS-induced hyperactivation of KLE cells, as demonstrated by reduced secretion of IL-1\u03b2, IL-6, IL-8, TNF-\u03b1 (24 h, 48 h, and 72 h) and CCL2, CCL3, and CLL5 (p &lt; 0.05). Furthermore, MiodesinTM also inhibited mRNA expression and secretion of matrix metalloproteinase-2 (MMP-2), MMP-9, and vascular endothelial growth factor (VEGF), which are key regulators of invasion of endometrial cells. Thus, the study concludes that MiodesinTM presents beneficial effects in the context of endometriosis, positively affecting the inflammatory and proliferative response.<\/jats:p>","DOI":"10.3390\/molecules27030782","type":"journal-article","created":{"date-parts":[[2022,1,25]],"date-time":"2022-01-25T20:40:18Z","timestamp":1643143218000},"page":"782","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["MiodesinTM Positively Modulates the Immune Response in Endometrial and Vaginal Cells"],"prefix":"10.3390","volume":"27","author":[{"given":"Carlos Rocha","family":"Oliveira","sequence":"first","affiliation":[{"name":"School of Medicine, Anhembi Morumbi University, Avenida Deputado Benedito Matarazzo 6070, Sao Jose dos Campos 12231-280, SP, Brazil"},{"name":"Postgraduate Program in Biomedical Engineering, Department of Science and Technology, Federal University of Sao Paulo (UNIFESP), Rua Talim, 330, Vila Nair, Sao Jose dos Campos 12231-280, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2119-3660","authenticated-orcid":false,"given":"Hudson","family":"Polonini","sequence":"additional","affiliation":[{"name":"Fagron BV, Fascinatio Boulevard 350, 3065 WB Rotterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8065-5618","authenticated-orcid":false,"given":"Maria Cristina","family":"Marcucci","sequence":"additional","affiliation":[{"name":"Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, Universidade Estadual Paulista-UNESP, Sao Jose dos Campos 12231-280, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6379-1143","authenticated-orcid":false,"given":"Rodolfo P.","family":"Vieira","sequence":"additional","affiliation":[{"name":"Post-graduation Program in Sciences of Human Movement and Rehabilitation, Federal University of Sao Paulo (UNIFESP), Avenida Ana Costa 95, Santos 11060-001, SP, Brazil"},{"name":"Post-Graduation Program in Human Movement and Rehabilitation, Unievangelica, Avenida Universit\u00e1ria KM 3,5, Anapolis 75083-515, GO, Brazil"},{"name":"Brazilian Institute of Teaching and Research in Pulmonary and Exercise Immunology (IBEPIPE), Rua Pedro Ernesto 240, Sao Jose dos Campos 12231-280, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,1,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1016\/j.jogc.2019.10.031","article-title":"Influence of Ethnicity on Clinical Presentation and Quality of Life in Women with Uterine Fibroids: Results from a Prospective Observational Registry","volume":"42","author":"Murji","year":"2020","journal-title":"J. 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