{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T17:45:57Z","timestamp":1792431957848,"version":"4.5.0"},"reference-count":43,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2022,2,3]],"date-time":"2022-02-03T00:00:00Z","timestamp":1643846400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The transient vanilloid receptor potential type 1 (TRPV1) regulates neuronal and vascular functions mediated by nitric oxide (NO) and by the calcitonin gene-related peptide (CGRP). Here, we study the participation of TRPV1 in the regulation of myocardial injury caused by ischemia-reperfusion and in the control of NO, tetrahydrobiopterin (BH4), the cGMP pathway, CGRP, total antioxidant capacity (TAC), malondialdehyde (MDA) and phosphodiesterase-3 (PDE-3). Isolated hearts of Wistar rats perfused according to the Langendorff technique were used to study the effects of an agonist of TRPV1, capsaicin (CS), an antagonist, capsazepine (CZ), and their combination CZ+CS. The hearts were subjected to three conditions: (1) control, (2) ischemia and (3) ischemia-reperfusion. We determined cardiac mechanical activity and the levels of NO, cGMP, BH4, CGRP, TAC, MDA and PDE-3 in ventricular tissue after administration of CS, CZ and CZ+CS. Western blots were used to study the expressions of eNOS, iNOS and phosphorylated NOS (pNOS). Structural changes were determined by histological evaluation. CS prevented damage caused by ischemia-reperfusion by improving cardiac mechanical activity and elevating the levels of NO, cGMP, BH4, TAC and CGRP. TRPV1 and iNOS expression were increased under ischemic conditions, while eNOS and pNOS were not modified. We conclude that the activation of TRPV1 constitutes a therapeutic possibility to counteract the damage caused by ischemia and reperfusion by regulating the NO pathway through CGRP.<\/jats:p>","DOI":"10.3390\/molecules27031031","type":"journal-article","created":{"date-parts":[[2022,2,3]],"date-time":"2022-02-03T05:42:33Z","timestamp":1643866953000},"page":"1031","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["TRPV1 Contributes to Modulate the Nitric Oxide Pathway and Oxidative Stress in the Isolated and Perfused Rat Heart during Ischemia and Reperfusion"],"prefix":"10.3390","volume":"27","author":[{"given":"Vicente","family":"Castrej\u00f3n-T\u00e9llez","sequence":"first","affiliation":[{"name":"Departamento de Fisiolog\u00eda, Ciudad de M\u00e9xico 14080, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2999-1050","authenticated-orcid":false,"given":"Leonardo","family":"del Valle-Mondrag\u00f3n","sequence":"additional","affiliation":[{"name":"Departamento de Farmacolog\u00eda Dr. Rafael M\u00e9ndez Mart\u00ednez, Ciudad de M\u00e9xico 14080, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6510-2954","authenticated-orcid":false,"given":"Israel","family":"P\u00e9rez-Torres","sequence":"additional","affiliation":[{"name":"Departamento de Biomedicina Cardiovascular, Ciudad de M\u00e9xico 14080, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2655-7590","authenticated-orcid":false,"given":"Ver\u00f3nica","family":"Guarner-Lans","sequence":"additional","affiliation":[{"name":"Departamento de Fisiolog\u00eda, Ciudad de M\u00e9xico 14080, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo","family":"Pastel\u00edn-Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Departamento de Farmacolog\u00eda Dr. Rafael M\u00e9ndez Mart\u00ednez, Ciudad de M\u00e9xico 14080, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ang\u00e9lica","family":"Ruiz-Ram\u00edrez","sequence":"additional","affiliation":[{"name":"Departamento de Biomedicina Cardiovascular, Ciudad de M\u00e9xico 14080, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julieta Anabell","family":"D\u00edaz-Ju\u00e1rez","sequence":"additional","affiliation":[{"name":"Departamento de Farmacolog\u00eda Dr. Rafael M\u00e9ndez Mart\u00ednez, Ciudad de M\u00e9xico 14080, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elvira","family":"Varela-L\u00f3pez","sequence":"additional","affiliation":[{"name":"Laboratorio de Cardiolog\u00eda Translacional, Unidad de Investigaci\u00f3n en Medicina Translacional UNAM\/INCAR, Ciudad de M\u00e9xico 14080, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8910-5671","authenticated-orcid":false,"given":"V\u00edctor Hugo","family":"Oidor-Chan","sequence":"additional","affiliation":[{"name":"Departamento de Farmacolog\u00eda Dr. Rafael M\u00e9ndez Mart\u00ednez, Ciudad de M\u00e9xico 14080, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alvaro","family":"Vargas-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Departamento de Fisiolog\u00eda, Ciudad de M\u00e9xico 14080, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ra\u00fal","family":"Mart\u00ednez-Memije","sequence":"additional","affiliation":[{"name":"Departamento de Instrumentaci\u00f3n Electromec\u00e1nica, Instituto Nacional de Cardiolog\u00eda Ignacio Ch\u00e1vez, Ciudad de M\u00e9xico 14080, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro","family":"Flores-Ch\u00e1vez","sequence":"additional","affiliation":[{"name":"Departamento de Instrumentaci\u00f3n Electromec\u00e1nica, Instituto Nacional de Cardiolog\u00eda Ignacio Ch\u00e1vez, Ciudad de M\u00e9xico 14080, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4255-352X","authenticated-orcid":false,"given":"Bruno","family":"Le\u00f3n-Ru\u00edz","sequence":"additional","affiliation":[{"name":"Departamento de Atenci\u00f3n a la Salud, Universidad Aut\u00f3noma Metropolitana Unidad Xochimilco, Ciudad de M\u00e9xico 04960, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Arriaga-Carrillo","sequence":"additional","affiliation":[{"name":"Departamento de Atenci\u00f3n a la Salud, Universidad Aut\u00f3noma Metropolitana Unidad Xochimilco, Ciudad de M\u00e9xico 04960, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3669-7482","authenticated-orcid":false,"given":"Juan Carlos","family":"Torres-Narv\u00e1ez","sequence":"additional","affiliation":[{"name":"Departamento de Farmacolog\u00eda Dr. Rafael M\u00e9ndez Mart\u00ednez, Ciudad de M\u00e9xico 14080, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Zhao, C.N., Meng, X., Li, Y., Li, S., Liu, Q., Tang, G.Y., and Li, H.B. 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