{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T18:20:36Z","timestamp":1775499636887,"version":"3.50.1"},"reference-count":42,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2022,2,3]],"date-time":"2022-02-03T00:00:00Z","timestamp":1643846400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Witt Italia S.p.A.","award":["Not applicable"],"award-info":[{"award-number":["Not applicable"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Vitamin D (VD) is a calcium- and phosphate-controlling hormone used to treat bone disorders; yet, several other effects are progressively emerging. VD deficiency is highly prevalent worldwide, with suboptimal exposure to sunlight listed among the leading causes: oral supplementation with either cholecalciferol or calcitriol is used. However, there is a scarcity of clinical studies investigating how quickly VD concentrations can increase after supplementation. In this pilot study, the commercial supplement ImmuD3 (by Erboristeria Magentina\u00ae) was chosen as the source of VD and 2000 IU\/day was administered for one month to 21 healthy volunteers that had not taken any other VD supplements in the previous 30 days. Plasma VD levels were measured through liquid chromatography coupled to tandem mass spectrometry after 7, 14, and 28 days of supplementation. We found that 95% of the participants had insufficient VD levels at baseline (&lt;30 ng\/mL; median 23.72 ng\/mL; IQR 18.10\u201326.15), but after 28 days of supplementation, this percentage dropped to 62% (median 28.35 ng\/mL; IQR 25.78\u201335.20). The median increase in VD level was 3.09 ng\/mL (IQR 1.60\u20135.68) after 7 days and 8.85 ng\/mL (IQR 2.85\u201313.97F) after 28 days. This study suggests the need for continuing VD supplementation and for measuring target level attainment.<\/jats:p>","DOI":"10.3390\/molecules27031042","type":"journal-article","created":{"date-parts":[[2022,2,6]],"date-time":"2022-02-06T20:38:40Z","timestamp":1644179920000},"page":"1042","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Monthly Increase in Vitamin D Levels upon Supplementation with 2000 IU\/Day in Healthy Volunteers: Result from \u201cIntegriamoci\u201d, a Pilot Pharmacokinetic Study"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4102-5218","authenticated-orcid":false,"given":"Valeria","family":"Avataneo","sequence":"first","affiliation":[{"name":"Laboratory of Clinical Pharmacology and Pharmacogenetics, Department of Medical Sciences, Amedeo di Savoia Hospital, University of Turin, 10126 Turin, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0860-280X","authenticated-orcid":false,"given":"Alice","family":"Palermiti","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Pharmacology and Pharmacogenetics, Department of Medical Sciences, Amedeo di Savoia Hospital, University of Turin, 10126 Turin, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5973-9948","authenticated-orcid":false,"given":"Amedeo","family":"De Nicol\u00f2","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Pharmacology and Pharmacogenetics, Department of Medical Sciences, Amedeo di Savoia Hospital, University of Turin, 10126 Turin, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1977-9694","authenticated-orcid":false,"given":"Jessica","family":"Cusato","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Pharmacology and Pharmacogenetics, Department of Medical Sciences, Amedeo di Savoia Hospital, University of Turin, 10126 Turin, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gloria","family":"Giussani","sequence":"additional","affiliation":[{"name":"Witt Italia S.p.A., 10046 Poirino, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2534-8815","authenticated-orcid":false,"given":"Andrea","family":"Calcagno","sequence":"additional","affiliation":[{"name":"Unit of Infectious Diseases, Department of Medical Sciences, University of Turin, 10124 Turin, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1321-4126","authenticated-orcid":false,"given":"Antonio","family":"D\u2019Avolio","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Pharmacology and Pharmacogenetics, Department of Medical Sciences, Amedeo di Savoia Hospital, University of Turin, 10126 Turin, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1414","DOI":"10.1002\/ijc.25439","article-title":"Meta-analysis of observational studies of serum 25-hydroxyvitamin D levels and colorectal, breast and prostate cancer and colorectal adenoma","volume":"128","author":"Gandini","year":"2011","journal-title":"Int. J. Cancer"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"b5500","DOI":"10.1136\/bmj.b5500","article-title":"Association between pre-diagnostic circulating vitamin D concentration and risk of colorectal cancer in European populations:a nested case-control study","volume":"340","author":"Jenab","year":"2010","journal-title":"BMJ"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"3775","DOI":"10.1200\/JCO.2011.35.7566","article-title":"Association between vitamin D and risk of colorectal cancer: A systematic review of prospective studies","volume":"29","author":"Ma","year":"2011","journal-title":"J. Clin. Oncol."},{"key":"ref_4","first-page":"2515","article-title":"Vitamin D and cancer","volume":"26","author":"Spina","year":"2006","journal-title":"Anticancer Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1158\/1055-9965.EPI-09-0475","article-title":"Plasma 25-hydroxyvitamin D levels and the risk of colorectal cancer: The multiethnic cohort study","volume":"19","author":"Woolcott","year":"2010","journal-title":"Cancer Epidemiol. Biomark. Prev."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1016\/j.coph.2010.04.001","article-title":"Vitamin D: Modulator of the immune system","volume":"10","author":"Baeke","year":"2010","journal-title":"Curr. Opin. Pharmacol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1433","DOI":"10.1017\/S0007114511001991","article-title":"Vitamin D status has a linear association with seasonal infections and lung function in British adults","volume":"106","author":"Berry","year":"2011","journal-title":"Br. J. Nutr."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1017\/S0950268806007175","article-title":"Epidemic influenza and vitamin D","volume":"134","author":"Cannell","year":"2006","journal-title":"Epidemiol. Infect."},{"key":"ref_9","first-page":"4048","article-title":"Editorial\u2014Vitamin D status: A key modulator of innate immunity and natural defense from acute viral respiratory infections","volume":"24","author":"Fabbri","year":"2020","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1001\/archinternmed.2008.560","article-title":"Association between serum 25-hydroxyvitamin D level and upper respiratory tract infection in the Third National Health and Nutrition Examination Survey","volume":"169","author":"Ginde","year":"2009","journal-title":"Arch. Intern. Med."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1093\/ajcn\/86.3.714","article-title":"An association of serum vitamin D concentrations <40 nmol\/L with acute respiratory tract infection in young Finnish men","volume":"86","author":"Laaksi","year":"2007","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"i6583","DOI":"10.1136\/bmj.i6583","article-title":"Vitamin D supplementation to prevent acute respiratory tract infections: Systematic review and meta-analysis of individual participant data","volume":"356","author":"Martineau","year":"2017","journal-title":"BMJ"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Sabetta, J.R., DePetrillo, P., Cipriani, R.J., Smardin, J., Burns, L.A., and Landry, M.L. (2010). Serum 25-hydroxyvitamin d and the incidence of acute viral respiratory tract infections in healthy adults. PLoS ONE, 5.","DOI":"10.1371\/journal.pone.0011088"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1007\/s00394-010-0138-3","article-title":"Association of serum 25-hydroxyvitamin D with the risk of death in a general older population in Finland","volume":"50","author":"Virtanen","year":"2011","journal-title":"Eur. J. Nutr."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"DACH (2012). New reference values for vitamin D. Ann. Nutr. Metab., 60, 241\u2013246.","DOI":"10.1159\/000337547"},{"key":"ref_16","unstructured":"IOM (2021, November 23). US Institute of Medicine\u2014Dietary Reference Intakes for Calcium and Vitamin D, Available online: https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/21796828."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"EFSA (2012). Scientific Opinion on the Tolerable Upper Intake Level of vitamin D. EFSA J., 10, 2813.","DOI":"10.2903\/j.efsa.2012.2813"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1911","DOI":"10.1210\/jc.2011-0385","article-title":"Evaluation, treatment, and prevention of vitamin D deficiency: An Endocrine Society clinical practice guideline","volume":"96","author":"Holick","year":"2011","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_19","unstructured":"SACN (2021, October 18). SACN Vitamin D and Health Report, Available online: https:\/\/www.gov.uk\/government\/publications\/sacn-vitamin-d-and-health-report."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"246","DOI":"10.3389\/fendo.2018.00246","article-title":"Vitamin D Supplementation Guidelines for General Population and Groups at Risk of Vitamin D Deficiency in Poland-Recommendations of the Polish Society of Pediatric Endocrinology and Diabetes and the Expert Panel with Participation of National Specialist Consultants and Representatives of Scientific Societies-2018 Update","volume":"9","author":"Rusinska","year":"2018","journal-title":"Front. Endocrinol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1093\/ajcn\/77.1.204","article-title":"Human serum 25-hydroxycholecalciferol response to extended oral dosing with cholecalciferol","volume":"77","author":"Heaney","year":"2003","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1678S","DOI":"10.1093\/ajcn\/80.6.1678S","article-title":"Sunlight and vitamin D for bone health and prevention of autoimmune diseases, cancers, and cardiovascular disease","volume":"80","author":"Holick","year":"2004","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1359\/jbmr.090728","article-title":"Bone mineralization defects and vitamin D deficiency: Histomorphometric analysis of iliac crest bone biopsies and circulating 25-hydroxyvitamin D in 675 patients","volume":"25","author":"Priemel","year":"2010","journal-title":"J. Bone Miner. Res."},{"key":"ref_24","first-page":"1145","article-title":"Vitamin D: Current Guidelines and Future Outlook","volume":"38","author":"Pilz","year":"2018","journal-title":"Anticancer Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"550","DOI":"10.3389\/fendo.2018.00550","article-title":"Vitamin D Toxicity-A Clinical Perspective","volume":"9","author":"Lukaszkiewicz","year":"2018","journal-title":"Front. Endocrinol."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Aibana, O., Huang, C.C., Aboud, S., Arnedo-Pena, A., Becerra, M.C., Bellido-Blasco, J.B., Bhosale, R., Calderon, R., Chiang, S., and Contreras, C. (2019). Vitamin D status and risk of incident tuberculosis disease: A nested case-control study, systematic review, and individual-participant data meta-analysis. PLoS Med., 16.","DOI":"10.1371\/journal.pmed.1002907"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Esposito, S., and Lelii, M. (2015). Vitamin D and respiratory tract infections in childhood. BMC Infect. Dis., 15.","DOI":"10.1186\/s12879-015-1196-1"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"7190","DOI":"10.4049\/jimmunol.178.11.7190","article-title":"IFN-gamma- and TNF-independent vitamin D-inducible human suppression of mycobacteria: The role of cathelicidin LL-37","volume":"178","author":"Martineau","year":"2007","journal-title":"J. Immunol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"5314","DOI":"10.1128\/IAI.66.11.5314-5321.1998","article-title":"1,25-Dihydroxyvitamin D3 induces nitric oxide synthase and suppresses growth of Mycobacterium tuberculosis in a human macrophage-like cell line","volume":"66","author":"Rockett","year":"1998","journal-title":"Infect. Immun."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"35482","DOI":"10.1074\/jbc.M102876200","article-title":"1alpha,25-Dihydroxyvitamin D3-induced monocyte antimycobacterial activity is regulated by phosphatidylinositol 3-kinase and mediated by the NADPH-dependent phagocyte oxidase","volume":"276","author":"Sly","year":"2001","journal-title":"J. Biol. Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.chom.2009.08.004","article-title":"Vitamin D3 induces autophagy in human monocytes\/macrophages via cathelicidin","volume":"6","author":"Yuk","year":"2009","journal-title":"Cell Host Microbe"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1522","DOI":"10.5588\/ijtld.12.0122","article-title":"Risk and outcome of multidrug-resistant tuberculosis: Vitamin D receptor polymorphisms and serum 25(OH)D","volume":"16","author":"Rathored","year":"2012","journal-title":"Int. J. Tuberc. Lung Dis."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"350","DOI":"10.36118\/pharmadvances.2021.01","article-title":"Vitamin D in the COVID-19 prevention and treatment: Emerging evidence","volume":"3","author":"Isaia","year":"2021","journal-title":"Pharmadvances"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1879","DOI":"10.1007\/s40520-020-01650-9","article-title":"Associations between hypovitaminosis D and COVID-19: A narrative review","volume":"32","author":"Isaia","year":"2020","journal-title":"Aging Clin. Exp. Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"20191","DOI":"10.1038\/s41598-020-77093-z","article-title":"Analysis of vitamin D level among asymptomatic and critically ill COVID-19 patients and its correlation with inflammatory markers","volume":"10","author":"Jain","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Maghbooli, Z., Sahraian, M.A., Ebrahimi, M., Pazoki, M., Kafan, S., Tabriz, H.M., Hadadi, A., Montazeri, M., Nasiri, M., and Shirvani, A. (2020). Vitamin D sufficiency, a serum 25-hydroxyvitamin D at least 30 ng\/mL reduced risk for adverse clinical outcomes in patients with COVID-19 infection. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0239799"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Annweiler, G., Corvaisier, M., Gautier, J., Dubee, V., Legrand, E., Sacco, G., and Annweiler, C. (2020). Vitamin D Supplementation Associated to Better Survival in Hospitalized Frail Elderly COVID-19 Patients: The GERIA-COVID Quasi-Experimental Study. Nutrients, 12.","DOI":"10.3390\/nu12113377"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1136\/postgradmedj-2020-139065","article-title":"Short term, high-dose vitamin D supplementation for COVID-19 disease: A randomised, placebo-controlled, study (SHADE study)","volume":"98","author":"Rastogi","year":"2020","journal-title":"Postgrad. Med. J."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.bone.2013.10.014","article-title":"Pharmacokinetics of oral vitamin D(3) and calcifediol","volume":"59","author":"Jetter","year":"2014","journal-title":"Bone"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1092","DOI":"10.3945\/ajcn.2008.27189","article-title":"Vitamin D supplementation during Antarctic winter","volume":"89","author":"Smith","year":"2009","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1111\/jvh.12288","article-title":"Role of CYP27B1+2838 promoter polymorphism in the treatment of chronic hepatitis B HBeAg negative with PEG-interferon","volume":"22","author":"Boglione","year":"2014","journal-title":"J. Viral Hepat."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.bone.2018.10.006","article-title":"The genetics of vitamin D","volume":"126","author":"Jiang","year":"2018","journal-title":"Bone"}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/3\/1042\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:13:40Z","timestamp":1760134420000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/3\/1042"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,3]]},"references-count":42,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2022,2]]}},"alternative-id":["molecules27031042"],"URL":"https:\/\/doi.org\/10.3390\/molecules27031042","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,3]]}}}