{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T16:18:56Z","timestamp":1790266736164,"version":"4.1.0"},"reference-count":35,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2022,2,11]],"date-time":"2022-02-11T00:00:00Z","timestamp":1644537600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["DUT21RC(3)057"],"award-info":[{"award-number":["DUT21RC(3)057"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>This study developed a detection method based on the strategy of HPLC\/MS3 and verified its suitability by quantifying carbamazepine in human plasma. The high-performance liquid chromatography\u2013tandem mass spectrometry (HPLC\/MS3) system was performed using a Shimadzu UFLC XR liquid chromatography and a SCIEX QTRAP\u00ae 5500 linear ion trap triple quadrupole mass spectrometer. The specific operation was as follows: the sample protein was firstly precipitated using methanol, then carbamazepine and carbamazepine-D2N15 were separated on an ACQUITY UPLC HSS T3 column using the gradient elution with solvent A (0.1% formic acid) and solvent B (0.1% formic acid in acetonitrile) at a flow rate of 0.25 mL\/min. Each sample was run for 7 min. This method was validated for various parameters including accuracy, precision, selectivity, linearity, LLOQ, etc. Only 5 \u03bcL of sample plasma could obtain the result of LLOD 0.5 \u00b5g\/mL. The intra-day and inter-day precision was &lt;8.23%, and accuracy was between \u22121.74% and 2.92%. This method was successfully used for monitoring the blood concentration of epilepsy patients after carbamazepine treatment.<\/jats:p>","DOI":"10.3390\/molecules27041224","type":"journal-article","created":{"date-parts":[[2022,2,13]],"date-time":"2022-02-13T20:34:45Z","timestamp":1644784485000},"page":"1224","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["LC\u2013MS3 Strategy for Quantification of Carbamazepine in Human Plasma and Its Application in Therapeutic Drug Monitoring"],"prefix":"10.3390","volume":"27","author":[{"given":"Dongxiao","family":"Ma","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, The First Hospital of Jilin University, Jilin University, Changchun 130021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengchao","family":"Ji","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The First Hospital of Jilin University, Jilin University, Changchun 130021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiwei","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The First Hospital of Jilin University, Jilin University, Changchun 130021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The First Hospital of Jilin University, Jilin University, Changchun 130021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Zeng","sequence":"additional","affiliation":[{"name":"The Bethune Institute of Epigenetic Medicine, The First Hospital of Jilin University, Jilin University, Changchun 130021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Yin","sequence":"additional","affiliation":[{"name":"School of Life and Pharmaceutical Sciences, Dalian University of Technology, Panjin 124221, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"(1967). 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