{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T22:23:37Z","timestamp":1788215017789,"version":"build-2803163510"},"reference-count":33,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2022,2,13]],"date-time":"2022-02-13T00:00:00Z","timestamp":1644710400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31902253"],"award-info":[{"award-number":["31902253"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20180921"],"award-info":[{"award-number":["BK20180921"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]},{"name":"individual technology research and development of modern agricultural industry of Jiangsu Province","award":["CX(19)3024"],"award-info":[{"award-number":["CX(19)3024"]}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2018M632399"],"award-info":[{"award-number":["2018M632399"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012246","name":"Priority Academic Program Development of Jiangsu Higher Education Institutions","doi-asserted-by":"publisher","award":["000000"],"award-info":[{"award-number":["000000"]}],"id":[{"id":"10.13039\/501100012246","id-type":"DOI","asserted-by":"publisher"}]},{"name":"earmarked fund for Jiangsu Agricultural Industry Technology System","award":["000000"],"award-info":[{"award-number":["000000"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Porcine pseudorabies (PR) is an important infectious disease caused by pseudorabies virus (PRV), which poses a major threat to food safety and security. Vaccine immunization has become the main means to prevent and control the disease. However, since 2011, a new PRV variant has caused huge economic losses to the Chinese pig industry. Panax notoginseng polysaccharides have immunomodulatory activity and other functions, but the antiviral effect has not been reported. We studied the anti-PRV activity of Panax notoginseng polysaccharides in vitro. A less cytopathic effect was observed by increasing the concentration of Panax notoginseng polysaccharides. Western blot, TCID50, plaque assay, and IFA revealed that Panax notoginseng polysaccharides could significantly inhibit the infectivity of PRV XJ5 on PK15 cells. In addition, we also found that Panax notoginseng polysaccharides blocked the adsorption and replication of PRV to PK15 cells in a dose-dependent manner. These results show that Panax notoginseng polysaccharides play an antiviral effect mainly by inhibiting virus adsorption and replication in vitro. Therefore, Panax notoginseng polysaccharides may be a potential anti-PRV agent.<\/jats:p>","DOI":"10.3390\/molecules27041254","type":"journal-article","created":{"date-parts":[[2022,2,13]],"date-time":"2022-02-13T20:34:45Z","timestamp":1644784485000},"page":"1254","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["The Antiviral Effect of Panax Notoginseng Polysaccharides by Inhibiting PRV Adsorption and Replication In Vitro"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9904-8893","authenticated-orcid":false,"given":"Changchao","family":"Huan","sequence":"first","affiliation":[{"name":"Institutes of Agricultural Science and Technology Development, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"},{"name":"Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China"},{"name":"Key Laboratory of Avian Bioproduct Development, Ministry of Agriculture and Rural Affairs, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Institutes of Agricultural Science and Technology Development, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"},{"name":"Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China"},{"name":"Key Laboratory of Avian Bioproduct Development, Ministry of Agriculture and Rural Affairs, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingting","family":"Yao","sequence":"additional","affiliation":[{"name":"Institutes of Agricultural Science and Technology Development, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"},{"name":"Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China"},{"name":"Key Laboratory of Avian Bioproduct Development, Ministry of Agriculture and Rural Affairs, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Ni","sequence":"additional","affiliation":[{"name":"China Animal Health and Epidemiology Center, Qingdao 266000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Song","family":"Gao","sequence":"additional","affiliation":[{"name":"Institutes of Agricultural Science and Technology Development, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"},{"name":"Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China"},{"name":"Key Laboratory of Avian Bioproduct Development, Ministry of Agriculture and Rural Affairs, Yangzhou 225009, China"},{"name":"Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1186\/s40425-018-0343-9","article-title":"Cytokine release syndrome","volume":"6","author":"Godel","year":"2018","journal-title":"J. Immunother. Cancer"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1080\/22221751.2018.1563459","article-title":"Pseudorabies virus: A neglected zoonotic pathogen in humans?","volume":"8","author":"Wong","year":"2019","journal-title":"Emerg. Microbes. Infect."},{"key":"ref_3","unstructured":"Pensaert, M. (1989). Virus Infections of Porcines, Elsevier."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2432","DOI":"10.1016\/j.vaccine.2015.03.094","article-title":"Construction of a triple gene-deleted Chinese Pseudorabies virus variant and its efficacy study as a vaccine candidate on suckling piglets","volume":"33","author":"Zhang","year":"2015","journal-title":"Vaccine"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"363","DOI":"10.4142\/jvs.2013.14.3.363","article-title":"Emergence of virulent pseudorabies virus infection in northern China","volume":"14","author":"Wu","year":"2013","journal-title":"J. Vet. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1749","DOI":"10.3201\/eid1911.130177","article-title":"Pseudorabies virus variant in Bartha-K61-vaccinated pigs, China, 2012","volume":"19","author":"An","year":"2013","journal-title":"Emerg. Infect. Dis."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1087","DOI":"10.3201\/eid2406.171612","article-title":"Human Endophthalmitis Caused By Pseudorabies Virus Infection, China, 2017","volume":"24","author":"Ai","year":"2018","journal-title":"Emerg. Infect. Dis."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"e3690","DOI":"10.1093\/cid\/ciaa987","article-title":"A novel human acute encephalitis caused by pseudorabies virus variant strain","volume":"73","author":"Liu","year":"2021","journal-title":"Clin. Infect. Dis"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.ijid.2019.08.007","article-title":"Characteristics of human encephalitis caused by pseudorabies virus: A case series study","volume":"87","author":"Yang","year":"2019","journal-title":"Int. J. Infect. Dis."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"160","DOI":"10.5142\/jgr.2010.34.3.160","article-title":"Chemical and pharmacological studies of saponins with a focus on American ginseng","volume":"34","author":"Yuan","year":"2010","journal-title":"J. Ginseng Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/j.jep.2016.05.005","article-title":"Traditional uses, botany, phytochemistry, pharmacology and toxicology of Panax notoginseng (Burk.) F.H. Chen: A review","volume":"188","author":"Wang","year":"2016","journal-title":"J. Ethnopharmacol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1411","DOI":"10.3389\/fphar.2019.01411","article-title":"Anti-Cancer Effects of Panax ginseng Berry Polysaccharides via Activation of Immune-Related Cells","volume":"10","author":"Lee","year":"2019","journal-title":"Front. Pharmacol"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1142\/S0192415X19500435","article-title":"Immunostimulating and Antimetastatic Effects of Polysaccharides Purified from Ginseng Berry","volume":"47","author":"Lee","year":"2019","journal-title":"Am. J. Chin. Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1016\/j.ijbiomac.2018.11.140","article-title":"The core structure characterization and of ginseng neutral polysaccharide with the immune-enhancing activity","volume":"123","author":"Li","year":"2019","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Ghosh, R., Bryant, D.L., and Farone, A.L. (2020). Panax quinquefolius (North American Ginseng) Polysaccharides as Immunomodulators: Current Research Status and Future Directions. Molecules, 25.","DOI":"10.3390\/molecules25245854"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1016\/j.jgr.2019.05.002","article-title":"Structural characteristics of a red ginseng acidic polysaccharide rhamnogalacturonan I with immunostimulating activity from red ginseng","volume":"44","author":"Lee","year":"2020","journal-title":"J. Ginseng. Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"169","DOI":"10.4196\/kjpp.2009.13.3.169","article-title":"Immunomodulatory activity of ginsan, a polysaccharide of panax ginseng, on dendritic cells","volume":"13","author":"Kim","year":"2009","journal-title":"Korean J. Physiol. Pharmacol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1002\/ptr.1262","article-title":"Panax notoginseng (Burk.) effects on fibrinogen and lipid plasma level in rats fed on a high-fat diet","volume":"17","author":"Cicero","year":"2003","journal-title":"Phytother. Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1055\/s-2004-815483","article-title":"Anti-inflammatory compounds of plant origin. Part II. modulation of pro-inflammatory cytokines, chemokines and adhesion molecules","volume":"70","author":"Calixto","year":"2004","journal-title":"Planta Med."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1542","DOI":"10.3389\/fimmu.2017.01542","article-title":"Protective Effect of Panax notoginseng Root Water Extract against Influenza A Virus Infection by Enhancing Antiviral Interferon-Mediated Immune Responses and Natural Killer Cell Activity","volume":"8","author":"Choi","year":"2017","journal-title":"Front. Immunol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"3049","DOI":"10.1016\/S0024-3205(02)01557-6","article-title":"A xylanase from roots of sanchi ginseng (Panax notoginseng) with inhibitory effects on human immunodeficiency virus-1 reverse transcriptase","volume":"70","author":"Lam","year":"2002","journal-title":"Life Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1055\/s-2006-962664","article-title":"Sanchinan-A, a reticuloendothelial system activating arabinogalactan from sanchi-ginseng (roots of Panax notoginseng)","volume":"53","author":"Ohtani","year":"1987","journal-title":"Planta Med."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1196","DOI":"10.1023\/A:1016060119425","article-title":"Immunostimulating polysaccharides from Panax notoginseng","volume":"13","author":"Gao","year":"1996","journal-title":"Pharm. Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1021\/np070323+","article-title":"Structural characterization and DPPH radical scavenging activity of an arabinoglucogalactan from Panax notoginseng root","volume":"71","author":"Wu","year":"2008","journal-title":"J. Nat. Prod."},{"key":"ref_25","first-page":"3433","article-title":"Administration of polysaccharide from Panax notoginseng prolonged the survival of H22 tumor-bearing mice","volume":"9","author":"Li","year":"2016","journal-title":"Onco. Targets Ther."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1016\/j.carbpol.2014.11.073","article-title":"An arabinogalactan from flowers of Panax notoginseng inhibits angiogenesis by BMP2\/Smad\/Id1 signaling","volume":"121","author":"Wang","year":"2015","journal-title":"Carbohydr. Polym."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1193","DOI":"10.1055\/s-2006-947222","article-title":"Immunoactive polysaccharide-rich fractions from Panax notoginseng","volume":"72","author":"Zhu","year":"2006","journal-title":"Planta Med."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"You, S., Shi, X., Yu, D., Zhao, D., An, Q., Wang, D., Zhang, J., Li, M., and Wang, C. (2021). Fermentation of Panax notoginseng root extract polysaccharides attenuates oxidative stress and promotes type I procollagen synthesis in human dermal fibroblast cells. BMC Complement. Med. Ther., 21.","DOI":"10.1186\/s12906-020-03197-8"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1084","DOI":"10.1016\/j.ijbiomac.2020.02.034","article-title":"A novel acidic polysaccharide from the residue of Panax notoginseng and its hepatoprotective effect on alcoholic liver damage in mice","volume":"149","author":"Wang","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.ijbiomac.2013.10.034","article-title":"Neuroprotective effect of Panax notoginseng plysaccharides against focal cerebral ischemia reperfusion injury in rats","volume":"63","author":"Jia","year":"2014","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1016\/j.virusres.2011.11.012","article-title":"Porcine circovirus type 2 explores the autophagic machinery for replication in PK-15 cells","volume":"163","author":"Zhu","year":"2012","journal-title":"Virus Res."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Gao, R., Zhang, Y., Kang, Y., Xu, W., Jiang, L., Guo, T., and Huan, C. (2020). Glycyrrhizin Inhibits PEDV Infection and Proinflammatory Cytokine Secretion via the HMGB1\/TLR4-MAPK p38 Pathway. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21082961"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1467","DOI":"10.1007\/s00705-017-3259-7","article-title":"Glycyrrhizin inhibits porcine epidemic diarrhea virus infection and attenuates the proinflammatory responses by inhibition of high mobility group box-1 protein","volume":"162","author":"Huan","year":"2017","journal-title":"Arch. Virol."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/4\/1254\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:18:40Z","timestamp":1760134720000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/4\/1254"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,13]]},"references-count":33,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2022,2]]}},"alternative-id":["molecules27041254"],"URL":"https:\/\/doi.org\/10.3390\/molecules27041254","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,13]]}}}