{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T15:56:42Z","timestamp":1792511802514,"version":"4.5.0"},"reference-count":50,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T00:00:00Z","timestamp":1645574400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100016047","name":"Science Fund of the Republic of Serbia","doi-asserted-by":"publisher","award":["6060592"],"award-info":[{"award-number":["6060592"]}],"id":[{"id":"10.13039\/501100016047","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100016047","name":"Science Fund of the Republic of Serbia","doi-asserted-by":"publisher","award":["7750168"],"award-info":[{"award-number":["7750168"]}],"id":[{"id":"10.13039\/501100016047","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000781","name":"European Research Council","doi-asserted-by":"publisher","award":["ERC-2016-CoG 725034"],"award-info":[{"award-number":["ERC-2016-CoG 725034"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Wild thyme (Thymus serpyllum L.) herbal dust has been recognized as a potential underutilized resource for the recovery of antioxidants. The aim of this paper was to optimize natural deep eutectic solvent (NADES) extraction of polyphenols to obtain improved antioxidant activity of extracts determined by selected in vitro assays (DPPH, FRAP, and ABTS). Twenty different NADES systems were investigated in the first step of the screening of the extraction solvent and l-proline (Pro)\u2013glycerine (Gly) based solvents provided the best results. Preliminary experiments organized by 25\u22121 fractional factorial design narrowed down the number of extraction factors from five (temperature, extraction time, NADES type, water content and L\/S ratio) to three and determined their experimental domain for the final step. A face-centered central composite design with temperature (40\u201355\u201370 \u00b0C), extraction time (60\u2013120\u2013180 min) and L\/S ratio (10\u201320\u201330 g NADES\/g sample) was applied for influence analysis and process optimization. Multi-response optimization suggested a temperature of 65 \u00b0C, time of extraction of 180 min and L\/S ratio of 28 g NADES\/g DW as optimal extraction parameters. Experimental validation confirmed good agreement between experimental and predicted results in the extract obtained at optimal conditions and the interactions in the most suitable NADES (N16; Pro\u2013Gly\u2013H2O; 1:2:1) were confirmed by the 1H-NMR.<\/jats:p>","DOI":"10.3390\/molecules27051508","type":"journal-article","created":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T22:54:02Z","timestamp":1645656842000},"page":"1508","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":104,"title":["Natural Deep Eutectic Solvent (NADES) Extraction Improves Polyphenol Yield and Antioxidant Activity of Wild Thyme (Thymus serpyllum L.) Extracts"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3551-7478","authenticated-orcid":false,"given":"Branimir","family":"Pavli\u0107","sequence":"first","affiliation":[{"name":"Faculty of Technology, University of Novi Sad, Blvd. cara Lazara 1, 21000 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8432-9951","authenticated-orcid":false,"given":"\u017divan","family":"Mrkonji\u0107","sequence":"additional","affiliation":[{"name":"Faculty of Technology, University of Novi Sad, Blvd. cara Lazara 1, 21000 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8219-1909","authenticated-orcid":false,"given":"Nemanja","family":"Tesli\u0107","sequence":"additional","affiliation":[{"name":"Institute of Food Technology, University of Novi Sad, Blvd. cara Lazara 1, 21000 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleksandra Cvetanovi\u0107","family":"Kljaki\u0107","sequence":"additional","affiliation":[{"name":"Faculty of Technology, University of Novi Sad, Blvd. cara Lazara 1, 21000 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milica","family":"Poji\u0107","sequence":"additional","affiliation":[{"name":"Institute of Food Technology, University of Novi Sad, Blvd. cara Lazara 1, 21000 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anamarija","family":"Mandi\u0107","sequence":"additional","affiliation":[{"name":"Institute of Food Technology, University of Novi Sad, Blvd. cara Lazara 1, 21000 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9762-5389","authenticated-orcid":false,"given":"Alena","family":"Stupar","sequence":"additional","affiliation":[{"name":"Institute of Food Technology, University of Novi Sad, Blvd. cara Lazara 1, 21000 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filipa","family":"Santos","sequence":"additional","affiliation":[{"name":"LAQV, REQUIMTE, Departamento de Qu\u00edmica, Nova School of Science and Technology, 2829-516 Caparica, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Rita C.","family":"Duarte","sequence":"additional","affiliation":[{"name":"LAQV, REQUIMTE, Departamento de Qu\u00edmica, Nova School of Science and Technology, 2829-516 Caparica, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3261-0332","authenticated-orcid":false,"given":"Aleksandra","family":"Mi\u0161an","sequence":"additional","affiliation":[{"name":"Institute of Food Technology, University of Novi Sad, Blvd. cara Lazara 1, 21000 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,23]]},"reference":[{"key":"ref_1","first-page":"101978","article-title":"Review of ethnobotanical, phytochemical, and pharmacological study of Thymus serpyllum L.","volume":"2015","year":"2015","journal-title":"Evid.-based Complement. 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