{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:09:49Z","timestamp":1790813389651,"version":"4.1.0"},"reference-count":30,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,3,4]],"date-time":"2022-03-04T00:00:00Z","timestamp":1646352000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Cyclodextrins (CDs) are cyclic oligosaccharides which can trap hydrophobic molecules and improve their chemical, physical, and biological properties. \u03b3-CD showed the highest aqueous solubility with the largest cavity diameter among other CD types. The current study describes a direct and easy method for nucleophilic mono-aminos to be substituted with \u03b3-CD and tested for their ability to host the guest curcumin (CUR) as a hydrophobic drug model. The mass spectrometry and NMR analyses showed the successful synthesis of three amino-modified \u03b3-CDs: mono-6-amino-6-deoxy-cyclodextrine (\u03b3-CD-NH2), mono-6-deoxy-6-ethanolamine-\u03b3-cyclodextrine (\u03b3-CD-NHCH2CH2OH), and mono-6-deoxy-6-aminoethylamino)-\u03b3-cyclodextrin (\u03b3-CD-NHCH2CH2NH2). These three amino-modified \u03b3-CDs were proven to be able to host CUR as native \u03b3-CDs with formation constants equal to 6.70 \u00b1 1.02, 5.85 \u00b1 0.80, and 8.98 \u00b1 0.90 mM\u22121, respectively. Moreover, these amino-modified \u03b3-CDs showed no significant toxicity against human dermal fibroblast cells. In conclusion, the current work describes a mono-substitution of amino-modified \u03b3-CDs that can still host guests and showed low toxicity in human dermal fibroblasts cells. Therefore, the amino-modified \u03b3-CDs can be used as a carrier host and be conjugated with a wide range of molecules for different biomedical applications, especially for active loading methods.<\/jats:p>","DOI":"10.3390\/molecules27051683","type":"journal-article","created":{"date-parts":[[2022,3,6]],"date-time":"2022-03-06T20:40:02Z","timestamp":1646599202000},"page":"1683","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Synthesis of Mono-Amino Substituted \u03b3-CD: Host\u2013Guest Complexation and In Vitro Cytotoxicity Investigation"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9341-2359","authenticated-orcid":false,"given":"Fadwa","family":"Odeh","sequence":"first","affiliation":[{"name":"Department of Chemistry, School of Science, The University of Jordan, Amman 11942, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fedaa","family":"Adaileh","sequence":"additional","affiliation":[{"name":"Department of Chemistry, School of Science, The University of Jordan, Amman 11942, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2946-7328","authenticated-orcid":false,"given":"Walhan","family":"Alshaer","sequence":"additional","affiliation":[{"name":"Cell Therapy Center, The University of Jordan, Amman 11942, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5916-5879","authenticated-orcid":false,"given":"Hamdi","family":"Nsairat","sequence":"additional","affiliation":[{"name":"Pharmacological and Diagnostic Research Centre, Faculty of Pharmacy, Al-Ahliyya Amman University, Amman 19328, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dana A.","family":"Alqudah","sequence":"additional","affiliation":[{"name":"Cell Therapy Center, The University of Jordan, Amman 11942, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Areej M.","family":"Jaber","sequence":"additional","affiliation":[{"name":"Pharmacological and Diagnostic Research Centre, Faculty of Pharmacy, Al-Ahliyya Amman University, Amman 19328, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2131-1791","authenticated-orcid":false,"given":"Abeer","family":"Al Bawab","sequence":"additional","affiliation":[{"name":"Department of Chemistry, School of Science, The University of Jordan, Amman 11942, Jordan"},{"name":"Hamdi Mango Center for Scientific Research, The University of Jordan, Amman 11942, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Wupper, S., L\u00fcersen, K., and Rimbach, G. 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