{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:42:02Z","timestamp":1790728922244,"version":"4.1.0"},"reference-count":56,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T00:00:00Z","timestamp":1646438400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Growing data suggest that Aspergillus niger, an endophytic fungus, is a rich source of natural compounds with a wide range of biological properties. This study aimed to examine the antimicrobial and antibiofilm capabilities of the Phragmites australis-derived endophyte against a set of pathogenic bacteria and fungi. The endophytic fungus Aspergillus sp. AP5 was isolated from the leaves of P. australis. The chemical profile of the fungal crude extract was identified by spectroscopic analysis using LC-HRESIMS. The fungal-derived extract was evaluated for its antimicrobial activity towards a set of pathogenic bacterial and fungal strains including Staphylococcus aureus, Pseudomonas aeruginosa, Proteus vulgaris, Klebsiella sp., Candida albicans, and Aspergillus niger. Moreover, antibiofilm activity toward four resistant biofilm-forming bacteria was also evaluated. Additionally, a neural-networking pharmacophore-based visual screening predicted the most probable bioactive compounds in the obtained extract. The AP5-EtOAc extract was found to have potent antibacterial activities against S. aureus, E. coli, and Klebsiella sp., while it exhibited low antibacterial activity toward P. Vulgaris and P. aeruginosa and displayed anticandidal activity. The AP5-EtOAc extract had significant antibiofilm activity in S. aureus, followed by P. aeruginosa. The active metabolites\u2019 antifungal and\/or antibacterial activities may be due to targeting the fungal CYP 51 and\/or the bacterial Gyr-B.<\/jats:p>","DOI":"10.3390\/molecules27051704","type":"journal-article","created":{"date-parts":[[2022,3,6]],"date-time":"2022-03-06T20:40:02Z","timestamp":1646599202000},"page":"1704","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["The Chemical Profiling, Docking Study, and Antimicrobial and Antibiofilm Activities of the Endophytic fungi Aspergillus sp. AP5"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9035-5638","authenticated-orcid":false,"given":"Mohamed A.","family":"Abdelgawad","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Chemistry, College of Pharmacy, Jouf University, Sakaka 72341, Aljouf, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9997-9284","authenticated-orcid":false,"given":"Ahmed A.","family":"Hamed","sequence":"additional","affiliation":[{"name":"Microbial Chemistry Department, National Research Centre, 33 El-Buhouth Street, Dokki, Giza 12622, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0531-5194","authenticated-orcid":false,"given":"AbdElAziz A.","family":"Nayl","sequence":"additional","affiliation":[{"name":"Department of chemistry, College of Science, Jouf University, Sakaka 72341, Aljouf, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6431-279X","authenticated-orcid":false,"given":"Mona Shaban E. M.","family":"Badawy","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Faculty of Pharmacy (Girls), Al-Azhar University, Cairo 11754, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9179-4373","authenticated-orcid":false,"given":"Mohammed M.","family":"Ghoneim","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Practice, College of Pharmacy, AlMaarefa University, Ad Diriyah 13713, Riyadh, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1442-183X","authenticated-orcid":false,"given":"Ahmed M.","family":"Sayed","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, Nahda University, Beni-Suef 62513, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0174-4434","authenticated-orcid":false,"given":"Hossam M.","family":"Hassan","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62513, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0611-651X","authenticated-orcid":false,"given":"Noha M.","family":"Gamaleldin","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Pharmacy, The British University in Egypt (BUE), Cairo 11837, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,5]]},"reference":[{"key":"ref_1","first-page":"1","article-title":"The utilisation of reed (Phragmites australis): A review","volume":"13","author":"Thevs","year":"2013","journal-title":"Mires Peat."},{"key":"ref_2","first-page":"179","article-title":"Genetic diversity, ecophysiology and growth dynamics of reed (Phragmites australis)","volume":"64","author":"Brix","year":"1999","journal-title":"Aquat. Bot."},{"key":"ref_3","first-page":"74","article-title":"Materials from excursion flora of Egypt (EFE)","volume":"15","author":"Fayed","year":"1995","journal-title":"Taeckholmia"},{"key":"ref_4","unstructured":"Zahran, M.A., and Willis, A.J. (2009). The Vegetation of Egypt, Springer. [2nd ed.]."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1007\/s00248-006-9193-y","article-title":"Novel and unexpected prokaryotic diversity in water and sediments of the alkaline, hypersaline lakes of the Wadi AN Natrun, Egypt","volume":"54","author":"Mesbah","year":"2007","journal-title":"Microb. Ecol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1007\/BF02442128","article-title":"Inland saline lakes of Wadi El Natrun depression, Egypt","volume":"8","author":"Taher","year":"1999","journal-title":"Int. J. Salt Lake Res."},{"key":"ref_7","first-page":"507","article-title":"Grasses in ancient Egypt","volume":"62","author":"Boulos","year":"2007","journal-title":"Kew Bull."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"972","DOI":"10.1021\/np070447m","article-title":"Cytotoxic metabolites from the fungal endophyte Alternaria sp. and their subsequent detection in its host plant Polygonum senegalense","volume":"71","author":"Aly","year":"2008","journal-title":"J. Nat. Prod."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1528","DOI":"10.1080\/13880209.2017.1309054","article-title":"Endophytic fungi associated with Monarda citriodora, an aromatic and medicinal plant, and their biocontrol potential","volume":"1","author":"Katoch","year":"2017","journal-title":"Pharm. Biol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.micpath.2015.04.001","article-title":"Fungal endophytes as prolific source of phytochemicals and other bioactive natural products: A review","volume":"82","author":"Nisa","year":"2015","journal-title":"Microb. Pathog."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1080\/07060660109506965","article-title":"Microbial gifts from the rain forest","volume":"24","author":"Strobel","year":"2002","journal-title":"Can. J. Plant Pathol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1039\/b100918o","article-title":"Endophytes: A rich source of functional metabolites","volume":"18","author":"Tan","year":"2001","journal-title":"Nat. Prod. Rep."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/j.fitote.2018.05.033","article-title":"An endophytic Fungi of Ginkgo biloba L. produces antimicrobial metabolites as potential inhibitors of FtsZ of Staphylococcus aureus","volume":"128","author":"Wu","year":"2018","journal-title":"Fitoterapia"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1111\/j.1574-695X.2002.tb00602.x","article-title":"Brefeldin A, a cytotoxin produced by Paecilomyces sp. and Aspergillus clavatus isolated from Taxus mairei and Torreya grandis","volume":"34","author":"Wang","year":"2002","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1016\/j.cclet.2007.05.054","article-title":"A New naphthoquinoneimine derivative from the marinealgal-derived endophytic fungus Aspergillus niger EN13","volume":"18","author":"Zhang","year":"2007","journal-title":"Chin. Chem. Lett."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"5677","DOI":"10.1016\/j.bmcl.2010.08.024","article-title":"Indoloditerpenes from an algicolous isolate of Aspergillus oryzae","volume":"20","author":"Qiao","year":"2010","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1248\/cpb.52.375","article-title":"Golmaenone, a New Diketopiperazin Alkaloid from the Marine-Derived Fungus Aspergillus sp.","volume":"52","author":"Li","year":"2004","journal-title":"Chem. Pharm. Bull."},{"key":"ref_18","unstructured":"Carvalho, C.M. (2005). Recursos Naturais Amaz\u00f4nicos com Perspectivas de uso Biotecnol\u00f3gico Sobre o Mycobacterium tuberculosis. [Master\u2019s Dissertation, Instituto de Pesquisas Tecnol\u00f3gicas, Instituto Butantan, Universidade de S\u00e3o Paulo]."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1146\/annurev.mi.41.100187.002251","article-title":"Bacterial biofilms in nature and disease","volume":"41","author":"Costerton","year":"1987","journal-title":"Annu. Rev. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1318","DOI":"10.1126\/science.284.5418.1318","article-title":"Bacterial biofilms: A common cause of persistent infections","volume":"284","author":"Costerton","year":"1999","journal-title":"Science"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1007\/s13205-017-0735-4","article-title":"Endophytic Nocardiopsis sp. from Zingiber officinale with both antiphytopathogenic mechanisms and antibiofilm activity against clinical isolates","volume":"7","author":"Sabu","year":"2017","journal-title":"3 Biotech"},{"key":"ref_22","unstructured":"Simmons, E.G. (2007). Alternaria: An Identification Manual, CBS Biodiversity Series 6, Centraalbureau voor Schimmelcultures."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"171","DOI":"10.3114\/sim0015","article-title":"Alternaria redefined","volume":"75","author":"Woudenberg","year":"2013","journal-title":"Stud. Mycol."},{"key":"ref_24","first-page":"512","article-title":"Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees","volume":"10","author":"Tamura","year":"1993","journal-title":"Mol. Biol. Evol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2770","DOI":"10.1038\/s41598-021-82201-8","article-title":"A metabolomic approach to target antimalarial metabolites in the Artemisia annua fungal endophytes","volume":"11","author":"Alhadrami","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"10361","DOI":"10.1039\/C9RA11021F","article-title":"Antibiofilm, antimicrobial and cytotoxic activity of extracellular, green-synthesized silver nanoparticles by two marine-derived actinomycete","volume":"10","author":"Hamed","year":"2020","journal-title":"RSC Adv."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"982","DOI":"10.1016\/j.biortech.2010.09.059","article-title":"Selection of non-conventional yeasts and their use in i obilized form for the bioremediation of olive oil mill wastewaters","volume":"102","author":"Bleve","year":"2011","journal-title":"Bioresour. Technol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1870","DOI":"10.1093\/molbev\/msw054","article-title":"MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets","volume":"33","author":"Kumar","year":"2016","journal-title":"Mol. Biol. Evol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"12558","DOI":"10.1073\/pnas.0605399103","article-title":"Broad-spectrum biofilm inhibition by a secreted bacterial polysaccharide","volume":"103","author":"Valle","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1225","DOI":"10.1007\/s00216-009-3081-5","article-title":"Review of secondary metabolites and mycotoxins from the Aspergillus niger group","volume":"395","author":"Nielsen","year":"2009","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1007\/s11046-006-0049-2","article-title":"Impact of Aspergillus oryzae genomics on industrial production of metabolites","volume":"162","author":"Abe","year":"2006","journal-title":"Mycopathologia"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"S88","DOI":"10.1080\/13693780802409542","article-title":"Effect of competition on the production and activity of secondary metabolites in Aspergillus species","volume":"47","author":"Losada","year":"2009","journal-title":"Med. Mycol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1038\/ja.2014.130","article-title":"Characterization of four new antifungal yanuthones from Aspergillus niger","volume":"68","author":"Petersen","year":"2015","journal-title":"J. Antibiot."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1787","DOI":"10.1021\/np200381u","article-title":"Nigerapyrones A\u2013H, \u03b1-pyrone derivatives from the marine mangrove-derived endophytic fungus Aspergillus niger MA-132","volume":"74","author":"Liu","year":"2021","journal-title":"J. Nat. Prod."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"4743","DOI":"10.1021\/jo00281a010","article-title":"Tubingensin A: An antiviral carbazole alkaloid from the sclerotia of Aspergillus tubingensis","volume":"54","author":"TePaske","year":"1989","journal-title":"J. Org. Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1038\/ja.2007.15","article-title":"Carbonarones A and B, new bioactive \u03b3-pyrone and \u03b1-pyridone derivatives from the marine-derived fungus Aspergillus carbonarius","volume":"60","author":"Zhang","year":"2007","journal-title":"J. Antibiot."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1038\/ja.2007.24","article-title":"Nigerasperones A~C, new monomeric and dimeric naphtho-\u03b3-pyrones from a marine alga-derived endophytic fungus Aspergillus niger EN-13","volume":"60","author":"Zhang","year":"2007","journal-title":"J. Antibiot."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"7078","DOI":"10.1021\/jo970568z","article-title":"Asperazine, a selective cytotoxic alkaloid from a sponge-derived culture of Aspergillus niger","volume":"62","author":"Varoglu","year":"1997","journal-title":"J. Org. Chem."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/j.bmcl.2015.12.005","article-title":"Aspernigrins with anti-HIV-1 activities from the marine-derived fungus Aspergillus niger SCSIO Jcsw6F30","volume":"26","author":"Zhou","year":"2016","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"5457","DOI":"10.1021\/jo00258a011","article-title":"Antiinsectan aflavinine derivatives from the sclerotia of Aspergillus flavus","volume":"53","author":"Gloer","year":"1988","journal-title":"J. Org. Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2289","DOI":"10.1021\/ol010089t","article-title":"Total synthesis of nafuredin, a selective NADH-fumarate reductase inhibitor","volume":"3","author":"Takano","year":"2001","journal-title":"Org. Lett."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1038\/ja.2006.67","article-title":"Tensidols, new potentiators of antifungal miconazole activity, produced by Aspergillus niger FKI-2342","volume":"59","author":"Fukuda","year":"2006","journal-title":"J. Antibiot."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"808","DOI":"10.1038\/ja.2006.108","article-title":"Atromentin and leucomelone, the first inhibitors specific to enoyl-ACP reductase (FabK) of Streptococcus pneumoniae","volume":"59","author":"Zheng","year":"2006","journal-title":"J. Antibiot."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1901862","DOI":"10.1002\/adhm.201901862","article-title":"Artificial intelligence and machine learning in computational nanotoxicology: Unlocking and empowering nanomedicine","volume":"9","author":"Singh","year":"2020","journal-title":"Adv. Healthc. Mater."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2000084","DOI":"10.1002\/aisy.202000084","article-title":"Artificial Intelligence and Machine Learning Empower Advanced Biomedical Material Design to Toxicity Prediction","volume":"2","author":"Singh","year":"2020","journal-title":"Adv. Intell. Syst."},{"key":"ref_47","first-page":"311","article-title":"Machine-Learning-Based Approach to Decode the Influence of Nanomaterial Properties on Their Interaction with Cells","volume":"11","author":"Singh","year":"2020","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Musa, A., Shady, N., Ahmed, S., Alnusaire, T., Sayed, A., Alowaiesh, B., Sabouni, I., Al-Sanea, M., Mostafa, E., and Youssif, K. (2021). Antiulcer Potential of Olea europea L. cv. Arbequina Leaf Extract Supported by Metabolic Profiling and Molecular Docking. Antioxidants, 10.","DOI":"10.3390\/antiox10050644"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Alzarea, S.I., Elmaidomy, A.H., Saber, H., Musa, A., Al-Sanea, M.M., Mostafa, E.M., Hendawy, O.M., Youssif, K.A., Alanazi, A.S., and Alharbi, M. (2021). Potential Anticancer Lipoxygenase Inhibitors from the Red Sea-Derived Brown Algae Sargassum cinereum: An In-Silico-Supported In-Vitro Study. Antibiotics, 10.","DOI":"10.3390\/antibiotics10040416"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"152986","DOI":"10.1016\/j.tetlet.2021.152986","article-title":"New glucose-6-phosphate dehydrogenase inhibitor from the Red Sea sponge Echinoclathria sp.","volume":"72","author":"Abdelhameed","year":"2021","journal-title":"Tetrahedron Lett."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"18009","DOI":"10.1039\/D1RA01725J","article-title":"Green-synthesized zinc oxide nanoparticles, anti-Alzheimer potential and the metabolic profiling of Sabal blackburniana grown in Egypt supported by molecular modelling","volume":"11","author":"Saber","year":"2021","journal-title":"RSC Adv."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Gamaleldin, N.M., Bakeer, W., Sayed, A.M., Shamikh, Y.I., El-Gendy, A.O., Hassan, H.M., Horn, H., Abdelmohsen, U.R., and Hozzein, W.N. (2020). Exploration of Chemical Diversity and Antitrypanosomal Activity of Some Red Sea-Derived Actinomycetes Using the OSMAC Approach Supported by LC-MS-Based Metabolomics and Molecular Modelling. Antibiotics, 9.","DOI":"10.3390\/antibiotics9090629"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1016\/j.bcp.2007.04.021","article-title":"Phospholipase A2 as targets for anti-cancer drugs","volume":"74","author":"Cummings","year":"2007","journal-title":"Biochem. Pharmacol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"W393","DOI":"10.1093\/nar\/gks496","article-title":"idTarget: A web server for identifying protein targets of small chemical molecules with robust scoring functions and a divide-and-conquer docking approach","volume":"40","author":"Wang","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"6728","DOI":"10.1074\/jbc.M117.778308","article-title":"Structural analyses of Candida albicans sterol 14\u03b1-demethylase complexed with azole drugs address the molecular basis of azole-mediated inhibition of fungal sterol biosynthesis","volume":"292","author":"Hargrove","year":"2017","journal-title":"J. Biol. Chem."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"10145","DOI":"10.1021\/acsomega.0c00865","article-title":"Lead identification of 8-(methylamino)-2-oxo-1,2-dihydroquinoline derivatives as DNA gyrase inhibitors: Hit-to-lead generation involving thermodynamic evaluation","volume":"5","author":"Ushiyama","year":"2020","journal-title":"ACS Omega"}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/5\/1704\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:32:35Z","timestamp":1760135555000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/5\/1704"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,5]]},"references-count":56,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2022,3]]}},"alternative-id":["molecules27051704"],"URL":"https:\/\/doi.org\/10.3390\/molecules27051704","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3,5]]}}}