{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T19:50:39Z","timestamp":1790884239131,"version":"4.1.0"},"reference-count":34,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2022,3,17]],"date-time":"2022-03-17T00:00:00Z","timestamp":1647475200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Union","doi-asserted-by":"publisher","award":["765115"],"award-info":[{"award-number":["765115"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["Lorraine universit\u00e9 d'excellence"],"award-info":[{"award-number":["Lorraine universit\u00e9 d'excellence"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"name":"R\u00e9gion Grand Est","award":["FRCR"],"award-info":[{"award-number":["FRCR"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Bryophytes produce rare and bioactive compounds with a broad range of therapeutic potential, and many species are reported in ethnomedicinal uses. However, only a few studies have investigated their potential as natural anti-inflammatory drug candidate compounds. The present study investigates the anti-inflammatory effects of thirty-two species of bryophytes, including mosses and liverworts, on Raw 264.7 murine macrophages stimulated with lipopolysaccharide (LPS) or recombinant human peroxiredoxin (hPrx1). The 70% ethanol extracts of bryophytes were screened for their potential to reduce the production of nitric oxide (NO), an important pro-inflammatory mediator. Among the analyzed extracts, two moss species significantly inhibited LPS-induced NO production without cytotoxic effects. The bioactive extracts of Dicranum majus and Thuidium delicatulum inhibited NO production in a concentration-dependent manner with IC50 values of 1.04 and 1.54 \u00b5g\/mL, respectively. The crude 70% ethanol and ethyl acetate extracts were then partitioned with different solvents in increasing order of polarity (n-hexane, diethyl ether, chloroform, ethyl acetate, and n-butanol). The fractions were screened for their inhibitory effects on NO production stimulated with LPS at 1 ng\/mL or 10 ng\/mL. The NO production levels were significantly affected by the fractions of decreasing polarity such as n-hexane and diethyl ether ones. Therefore, the potential of these extracts to inhibit the LPS-induced NO pathway suggests their effective properties in attenuating inflammation and could represent a perspective for the development of innovative therapeutic agents.<\/jats:p>","DOI":"10.3390\/molecules27061940","type":"journal-article","created":{"date-parts":[[2022,3,20]],"date-time":"2022-03-20T21:34:27Z","timestamp":1647812067000},"page":"1940","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Anti-Inflammatory Activity of Bryophytes Extracts in LPS-Stimulated RAW264.7 Murine Macrophages"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6959-640X","authenticated-orcid":false,"given":"Ra\u00edssa Volpatto","family":"Marques","sequence":"first","affiliation":[{"name":"Department of Biotechnology and Biomedicine, Technical University of Denmark, S\u00f8ltoft Plads 223, 2800 Kongens Lyngby, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6273-6118","authenticated-orcid":false,"given":"Stefania Enza","family":"Sestito","sequence":"additional","affiliation":[{"name":"UMR 7365 CNRS, Ing\u00e9nierie Mol\u00e9culaire et Physiopathologie Articulaire IMoPA, Universit\u00e9 de Lorraine, 54500 Vand\u0153uvre-l\u00e8s-Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9898-2625","authenticated-orcid":false,"given":"Fr\u00e9d\u00e9ric","family":"Bourgaud","sequence":"additional","affiliation":[{"name":"Plant Advanced Technologies, 19 Avenue de la For\u00eat de Haye, 54500 Vand\u0153uvre-l\u00e8s-Nancy, France"},{"name":"Cellengo, 19 Avenue de la For\u00eat de Haye, 54500 Vand\u0153uvre-l\u00e8s-Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sissi","family":"Miguel","sequence":"additional","affiliation":[{"name":"Cellengo, 19 Avenue de la For\u00eat de Haye, 54500 Vand\u0153uvre-l\u00e8s-Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fr\u00e9d\u00e9ric","family":"Cailotto","sequence":"additional","affiliation":[{"name":"UMR 7365 CNRS, Ing\u00e9nierie Mol\u00e9culaire et Physiopathologie Articulaire IMoPA, Universit\u00e9 de Lorraine, 54500 Vand\u0153uvre-l\u00e8s-Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascal","family":"Reboul","sequence":"additional","affiliation":[{"name":"UMR 7365 CNRS, Ing\u00e9nierie Mol\u00e9culaire et Physiopathologie Articulaire IMoPA, Universit\u00e9 de Lorraine, 54500 Vand\u0153uvre-l\u00e8s-Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Yves","family":"Jouzeau","sequence":"additional","affiliation":[{"name":"UMR 7365 CNRS, Ing\u00e9nierie Mol\u00e9culaire et Physiopathologie Articulaire IMoPA, Universit\u00e9 de Lorraine, 54500 Vand\u0153uvre-l\u00e8s-Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5143-0105","authenticated-orcid":false,"given":"Sophie","family":"Rahuel-Clermont","sequence":"additional","affiliation":[{"name":"UMR 7365 CNRS, Ing\u00e9nierie Mol\u00e9culaire et Physiopathologie Articulaire IMoPA, Universit\u00e9 de Lorraine, 54500 Vand\u0153uvre-l\u00e8s-Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8962-4749","authenticated-orcid":false,"given":"Sandrine","family":"Boschi-Muller","sequence":"additional","affiliation":[{"name":"UMR 7365 CNRS, Ing\u00e9nierie Mol\u00e9culaire et Physiopathologie Articulaire IMoPA, Universit\u00e9 de Lorraine, 54500 Vand\u0153uvre-l\u00e8s-Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3070-807X","authenticated-orcid":false,"given":"Henrik Toft","family":"Simonsen","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Biomedicine, Technical University of Denmark, S\u00f8ltoft Plads 223, 2800 Kongens Lyngby, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6619-5769","authenticated-orcid":false,"given":"David","family":"Moulin","sequence":"additional","affiliation":[{"name":"UMR 7365 CNRS, Ing\u00e9nierie Mol\u00e9culaire et Physiopathologie Articulaire IMoPA, Universit\u00e9 de Lorraine, 54500 Vand\u0153uvre-l\u00e8s-Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1017\/S1479262116000320","article-title":"Bryophytes-an Emerging Source for Herbal Remedies and Chemical Production","volume":"14","author":"Ikram","year":"2016","journal-title":"Plant Genet. 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