{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T14:35:41Z","timestamp":1780497341380,"version":"3.54.1"},"reference-count":37,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2022,3,17]],"date-time":"2022-03-17T00:00:00Z","timestamp":1647475200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Our experiment determined the immunotropic activity of a natural, iridoid-anthocyanin extract from honeysuckle berry (Lonicera caerulea L.) (LC). The extract was administered to mice infected with Trichinella spiralis, orally at a dose of 2 g\/kg bw, six times at 24 h intervals (from day 3 prior to the infection to day 3 post-infection (dpi) with T. spiralis. At 5, 7, 14, and 21 dpi, samples of blood, spleen, and mesenteric lymph nodes (MLN) were collected, and isolated lymphocytes were analyzed by flow cytometry. The splenocyte proliferation was estimated with MTT testing, and the intensity of intestinal and muscle infection was also studied. LC stimulated the local immune system by inducing lymphocyte proliferation in the spleen 7 dpi and altered the percentage and absolute count of B (CD19+) and T (CD3+, CD8+) cells 7, 14, and 21 dpi in the peripheral blood. LC extract affected the dynamics of expulsion of adult Trichinella from the intestines and prolonged the intestinal phase of the infection but did not change the number of larvae in the muscles. These results suggest that Lonicera caerulea L. fruit extract modulates murine cellular immune response during intestinal phase of T. spiralis infection but shows no antiparasitic activity.<\/jats:p>","DOI":"10.3390\/molecules27061949","type":"journal-article","created":{"date-parts":[[2022,3,20]],"date-time":"2022-03-20T21:34:27Z","timestamp":1647812067000},"page":"1949","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Evaluation of Immunotropic Activity of Iridoid-Anthocyanin Extract of Honeysuckle Berries (Lonicera caerulea L.) in the Course of Experimental Trichinellosis in Mice"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8195-0790","authenticated-orcid":false,"given":"Jolanta","family":"Piekarska","sequence":"first","affiliation":[{"name":"Division of Parasitology, Department of Internal Medicine and Clinic of Horses, Dogs and Cats, Wroc\u0142aw University of Environmental and Life Sciences, Norwida 31, 50-375 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7526-3230","authenticated-orcid":false,"given":"Marianna","family":"Szczypka","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Wroc\u0142aw University of Environmental and Life Sciences, Norwida 31, 50-375 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Micha\u0142","family":"Gorczykowski","sequence":"additional","affiliation":[{"name":"Division of Parasitology, Department of Internal Medicine and Clinic of Horses, Dogs and Cats, Wroc\u0142aw University of Environmental and Life Sciences, Norwida 31, 50-375 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2785-2791","authenticated-orcid":false,"given":"Anna","family":"Sok\u00f3\u0142-\u0141\u0119towska","sequence":"additional","affiliation":[{"name":"Department of Fruit, Vegetable and Plant Nutraceutical Technology, Wroc\u0142aw University of Environmental and Life Sciences, Che\u0142mo\u0144skiego 37, 51-630 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2172-0408","authenticated-orcid":false,"given":"Alicja Z.","family":"Kucharska","sequence":"additional","affiliation":[{"name":"Department of Fruit, Vegetable and Plant Nutraceutical Technology, Wroc\u0142aw University of Environmental and Life Sciences, Che\u0142mo\u0144skiego 37, 51-630 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4","DOI":"10.2174\/187153012799279081","article-title":"Immunomodulation in trichinellosis: Does Trichinella really escape the host immune system?","volume":"12","author":"Bruschi","year":"2012","journal-title":"Endocr. Metab. Immune Disord. Drug Targets"},{"key":"ref_2","first-page":"523875","article-title":"Secretory products of Trichinella spiralis muscle larvae and immunomodulation: Implication for autoimmune diseases, allergies, and malignancies","volume":"2015","author":"Ilic","year":"2015","journal-title":"J. Immunol. Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.vetpar.2013.01.030","article-title":"Intestinal infection with Trichinella spiralis induces distinct, regional immune responses","volume":"194","author":"Blum","year":"2013","journal-title":"Vet. Parasitol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"195","DOI":"10.2478\/helm-2018-0015","article-title":"Modulation of TLR2 and TLR4 in macrophages following Trichinella Spiralis infection","volume":"55","author":"Han","year":"2018","journal-title":"Helminthologia"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1186\/s13567-020-0741-0","article-title":"Primary characterization of the immune response in pigs infected with Trichinella spiralis","volume":"51","author":"Wang","year":"2020","journal-title":"Vet. Res."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Go\u0142ba, M., Sok\u00f3\u0142-\u0141\u0119towska, A., and Kucharska, A.Z. (2020). Health properties and composition of honeysuckle berry Lonicera caerulea L. An Update on Recent Studies. Molecules, 25.","DOI":"10.3390\/molecules25030749"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1157","DOI":"10.3390\/molecules21091157","article-title":"Identification of iridoids in edible honeysuckle berries (Lonicera caerulea L. var. kamtschatica Sevast.) by UPLC-ESI-qTOF-MS\/MS","volume":"21","author":"Kucharska","year":"2016","journal-title":"Molecules"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Kucharska, A.Z., Sok\u00f3\u0142-\u0141\u0119towska, A., Oszmia\u0144ski, J., Pi\u00f3recki, N., and Fecka, I. (2017). Iridoids, phenolic compounds and antioxidant activity of edible honeysuckle berries (Lonicera caerulea var. kamtschatica Sevast.). Molecules, 22.","DOI":"10.3390\/molecules22030405"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1016\/j.biopha.2018.05.057","article-title":"Anti-tumor properties of anthocyanins from Lonicera caerulea \u2018Beilei\u2019 fruit on human hepatocellular carcinoma: In vitro and in vivo study","volume":"104","author":"Zhou","year":"2018","journal-title":"Biomed. Pharmacother."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"860","DOI":"10.1016\/j.exer.2005.10.024","article-title":"Effects of blue honeysuckle (Lonicera caerulea L.) extract on lipopolysaccharide-induced inflammation in vitro and in vivo","volume":"82","author":"Jin","year":"2006","journal-title":"Exp. Eye Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1555","DOI":"10.1016\/j.fct.2010.03.024","article-title":"Polyphenolic fraction of Lonicera caerulea L. fruits reduces oxidative stress and inflammatory markers induced by lipopolysaccharide in gingival fibroblasts","volume":"48","year":"2010","journal-title":"Food Chem. Toxicol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1600858","DOI":"10.1002\/mnfr.201600858","article-title":"Polyphenols from Lonicera caerulea L. berry attenuate experimental nonalcoholic steatohepatitis by inhibiting proinflammatory cytokines productions and lipid peroxidation","volume":"61","author":"Wu","year":"2017","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e1901315","DOI":"10.1002\/mnfr.201901315","article-title":"Lonicera caerulea L. polyphenols alleviate oxidative stress-induced intestinal environment imbalance and lipopolysaccharide-induced liver injury in HFD-fed rats by regulating the Nrf2\/HO-1\/NQO1 and MAPK pathways","volume":"64","author":"Li","year":"2020","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"39","DOI":"10.20463\/jenb.2018.0029","article-title":"Hepatoprotective and anti-obesity effects of Korean blue honeysuckle extracts in high fat diet-fed mice","volume":"22","author":"Chun","year":"2018","journal-title":"J. Exerc. Nutr. Biochem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"898","DOI":"10.1016\/j.nut.2012.11.006","article-title":"An anthocyanin-rich extract from Kamchatka honeysuckle increases enzymatic activity within the gut and ameliorates abnormal lipid and glucose metabolism in rats","volume":"29","year":"2013","journal-title":"Nutrition"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Wu, S., Hu, R., Nakano, H., Chen, K., Liu, M., He, X., Zhang, H., He, J., and Hou, D.X. (2018). Modulation of gut microbiota by Lonicera caerulea L. berry polyphenols in a mouse Model of fatty liver induced by high fat diet. Molecules, 23.","DOI":"10.3390\/molecules23123213"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.exppara.2018.03.012","article-title":"Effects of iridoid-anthocyanin extract of Cornus mas L. on hematological parameters, population and proliferation of lymphocytes during experimental infection of mice with Trichinella spiralis","volume":"188","author":"Piekarska","year":"2018","journal-title":"Exp. Parasitol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.intimp.2010.10.017","article-title":"Possible role of macrophages induced by an irridoid glycoside (RLJ-NE-299A) in host defense mechanism","volume":"11","author":"Sidiq","year":"2011","journal-title":"Int. Immunopharmacol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1016\/j.jep.2012.03.005","article-title":"In vitro and in vivo immunomodulatory activities of iridoids fraction from Barleria prionitis Linn","volume":"141","author":"Ghule","year":"2012","journal-title":"J. Ethnopharmacol."},{"key":"ref_20","first-page":"597","article-title":"Humoral and cellular immune responses induced in mice by purified iridoid mixture that inhibits penetration of Schistosoma mansoni cercariae upon topical treatment of mice tails","volume":"35","author":"Bahgat","year":"2005","journal-title":"J. Egypt Soc. Parasitol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.exppara.2016.02.001","article-title":"In vitro activity of Sorghum bicolor extracts, 3-deoxyanthocyanidins, against Toxoplasma gondii","volume":"164","author":"Abugri","year":"2016","journal-title":"Exp. Parasitol."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Dayar, E., Cebova, M., Lietava, J., Panghyova, E., and Pechanova, O. (2021). Antioxidant effect of Lonicera caerulea L. in the cardiovascular system of obese zucker rats. Antioxidants, 10.","DOI":"10.3390\/antiox10081199"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"145","DOI":"10.5582\/bst.2019.01005","article-title":"Methanol extract of Lonicera caerulea var. emphyllocalyx fruit has antibacterial and anti-biofilm activity against Streptococcus pyogenes in vitro","volume":"13","author":"Minami","year":"2019","journal-title":"Biosci. Trends"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Molina, A.K., Vega, E.N., Pereira, C., Dias, M.I., Heleno, S.A., Rodrigues, P., Fernandes, I.P., Barreiro, M.F., Kosti\u0107, M., and Sokovi\u0107, M. (2019). Promising antioxidant and antimicrobial food colourants from Lonicera caerulea L. var. Kamtschatica. Antioxidants, 8.","DOI":"10.3390\/antiox8090394"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1080\/09168451.2014.912115","article-title":"Cyanidin-3-glucoside suppresses Th2 cytokines and GATA-3 transcription factor in EL-4 T cells","volume":"78","author":"Pyo","year":"2014","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.jff.2018.02.023","article-title":"The potential health benefits of haskap (Lonicera caerulea L.): Role of cyanidin-3-O-glucoside","volume":"44","author":"Rupasinghe","year":"2018","journal-title":"J. Funct. Foods"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1797930","DOI":"10.1155\/2019\/1797930","article-title":"Effect of Lonicera caerulea var. emphyllocalyx extracts on murine Streptococcus pyogenes infection by modulating immune system","volume":"2019","author":"Minami","year":"2019","journal-title":"Biomed Res. Int."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"5133","DOI":"10.1021\/acs.jafc.7b01599","article-title":"Polyphenols from Lonicera caerulea L. berry inhibit LPS-Induced inflammation through dual modulation of inflammatory and antioxidant mediators","volume":"65","author":"Wu","year":"2017","journal-title":"J. Agric. Food Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.3390\/biom5021079","article-title":"Anti-Inflammatory activity of haskap cultivars is polyphenols-dependent","volume":"5","author":"Rupasinghe","year":"2015","journal-title":"Biomolecules"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"486","DOI":"10.4162\/nrp.2018.12.6.486","article-title":"Evaluation of in vitro anti-oxidant and anti-inflammatory activities of Korean and Chinese Lonicera caerulea","volume":"12","author":"Lee","year":"2018","journal-title":"Nutr. Res. Pract."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1007\/s11010-011-1046-4","article-title":"Regulation of cytokine expression in murine macrophages stimulated by excretory\/secretory products from Trichinella spiralis in vitro","volume":"360","author":"Bai","year":"2012","journal-title":"Mol. Cell. Biochem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"4267","DOI":"10.1039\/C6FO00627B","article-title":"Lonicera caerulea berry extract suppresses lipopolysaccharide-induced inflammation via Toll-like receptor and oxidative stress-associated mitogen-activated protein kinase signaling","volume":"7","author":"Wang","year":"2016","journal-title":"Food Funct."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.exppara.2013.02.014","article-title":"Systemic cytokine profiles and splenic toll-like receptor expression during Trichinella spiralis infection","volume":"134","author":"Yu","year":"2013","journal-title":"Exp. Parasitol."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Sok\u00f3\u0142-\u0141\u0119towska, A., Kucharska, A.Z., Hodun, G., and Go\u0142ba, M. (2020). Chemical composition of 21 cultivars of sour cherry (Prunus cerasus) fruit cultivated in Poland. Molecules, 25.","DOI":"10.3390\/molecules25194587"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"61","DOI":"10.5487\/TR.2015.31.1.061","article-title":"Single oral dose toxicity test of blue honeysuckle concentrate in mice","volume":"31","author":"Kim","year":"2015","journal-title":"Toxicol. Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"87","DOI":"10.4103\/0976-500X.72350","article-title":"Blood sample collection in small laboratory animals","volume":"1","author":"Parasuraman","year":"2010","journal-title":"J. Pharmacol. Pharmacother."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1166","DOI":"10.1111\/jphp.12760","article-title":"Propentofylline, phosphodiesterase and adenosine reuptake inhibitor modulates lymphocyte subsets and lymphocyte activity after in-vivo administration in non-immunized and SRBC-immunized mice","volume":"69","author":"Szczypka","year":"2017","journal-title":"J. Pharm. Pharmacol."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/6\/1949\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:38:16Z","timestamp":1760135896000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/6\/1949"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,17]]},"references-count":37,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2022,3]]}},"alternative-id":["molecules27061949"],"URL":"https:\/\/doi.org\/10.3390\/molecules27061949","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3,17]]}}}