{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T09:57:00Z","timestamp":1768816620594,"version":"3.49.0"},"reference-count":31,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,3,27]],"date-time":"2022-03-27T00:00:00Z","timestamp":1648339200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>A new biodegradable platform-based sensor for formaldehyde assay is proposed. Natural rubber latex was modified to polylactic acid\u2013chloroacetated natural rubber polymer blend sheets. The polymer blend sheet was grafted using a water-based system with amine monomers as a platform, with a spot exhibiting positive polarity for immobilizing with anionic dye (Acid Red 27). The sensor was exposed to formaldehyde. The color intensity of the dye on the sensor spot would decrease. Using a smartphone with image processing (via ImageJ program), the color intensity change (\u2206B) could be followed. A linear calibration, \u2206B intensity = 0.365 [FA] + 6.988, R2 = 0.997, was obtained for 10\u2013150 mM FA with LOD and LOQ at 3 and 10 mM, respectively (linear regression method). The precision was lower than 20% RSD. Application to real seafood samples was demonstrated. The ready-to-use sensor with the proposed method was cost-effective, was portable for on-site analysis, and demonstrated green chemical analysis.<\/jats:p>","DOI":"10.3390\/molecules27072159","type":"journal-article","created":{"date-parts":[[2022,3,27]],"date-time":"2022-03-27T21:29:36Z","timestamp":1648416576000},"page":"2159","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Modified Natural Rubber as a Simple Chemical Sensor with Smartphone Detection for Formaldehyde Content in a Seafood Sample"],"prefix":"10.3390","volume":"27","author":[{"given":"Chonnipa","family":"Yeerum","sequence":"first","affiliation":[{"name":"Department of Chemistry, Faculty of Science, King Mongkut\u2019s University of Technology Thonburi, Bangkok 10140, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9151-301X","authenticated-orcid":false,"given":"Piyanat","family":"Issarangkura Na Ayutthaya","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science, King Mongkut\u2019s University of Technology Thonburi, Bangkok 10140, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kullapon","family":"Kesonkan","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science, King Mongkut\u2019s University of Technology Thonburi, Bangkok 10140, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kanokwan","family":"Kiwfo","sequence":"additional","affiliation":[{"name":"Center of Excellence for Innovation in Analytical Science and Technology for Biodiversity-Based Economic and Society (I-ANALY-S-T_B.BES-CMU) and Department of Chemistry, Faculty of Sciences, Chiang Mai University, Chiang Mai 50200, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ploenpit","family":"Boochathum","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science, King Mongkut\u2019s University of Technology Thonburi, Bangkok 10140, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5871-8291","authenticated-orcid":false,"given":"Kate","family":"Grudpan","sequence":"additional","affiliation":[{"name":"Center of Excellence for Innovation in Analytical Science and Technology for Biodiversity-Based Economic and Society (I-ANALY-S-T_B.BES-CMU) and Department of Chemistry, Faculty of Sciences, Chiang Mai University, Chiang Mai 50200, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monnapat","family":"Vongboot","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science, King Mongkut\u2019s University of Technology Thonburi, Bangkok 10140, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,27]]},"reference":[{"key":"ref_1","unstructured":"International Agency for Research on Cancer (IARC) (2006). Formaldehyde, 2-Butoxyethanol and 1-tert- Butoxypropan-2-ol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1016\/j.foodchem.2008.10.027","article-title":"Rapid detection of formaldehyde concentration in food on a polydimethylsiloxane (PDMS) microfluidic chip","volume":"114","author":"Weng","year":"2009","journal-title":"Food Chem."},{"key":"ref_3","first-page":"87","article-title":"Formaldehyde in Seafood: A review","volume":"55","author":"Laly","year":"2018","journal-title":"Fish. Technol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"108882","DOI":"10.1016\/j.foodcont.2022.108882","article-title":"A review of the presence of formaldehyde in fish and seafood","volume":"136","author":"Jinadasa","year":"2022","journal-title":"Food Control"},{"key":"ref_5","first-page":"127","article-title":"Determination of formaldehyde in squid by high performance liquid chromatography","volume":"16","author":"Li","year":"2007","journal-title":"Asia Pac. J. Clin. Nutr."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1016\/j.foodchem.2011.08.056","article-title":"Determination of formaldehyde in fruit juice based on magnetic strong cation-exchange resin modified with 2,4-dinitrophenylhydrazine","volume":"131","author":"Wang","year":"2012","journal-title":"Food Chem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"3421","DOI":"10.1002\/jssc.201500420","article-title":"Extraction and preconcentration of formaldehyde in water by polypyrrole-coated magnetic nanoparticles and determination by high-performance liquid chromatography","volume":"38","author":"Safari","year":"2015","journal-title":"J. Sep. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1016\/j.foodchem.2016.05.069","article-title":"On-cartridge derivatisation using a calixarene solid-phase extraction sorbent for facile, sensitive and fast determination of formaldehyde in beer","volume":"211","author":"Deng","year":"2016","journal-title":"Food Chem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/j.jfda.2013.05.010","article-title":"Analysis of free and bound formaldehyde in squid and squid products by gas chromatography-mass spectrometry","volume":"21","author":"Yeh","year":"2013","journal-title":"J. Food Drug Anal."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1042\/bj0550416","article-title":"The colorimetric estimation of formaldehyde by means of the Hantzsch reaction","volume":"55","author":"Nash","year":"1953","journal-title":"Biochem. J."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1006\/fstl.1998.0380","article-title":"Comparison of the formaldehyde content found in boiled and raw mince of frozen saithe using different analytical methods","volume":"31","author":"Bechmann","year":"1998","journal-title":"Lebensm.-Wiss. Technol."},{"key":"ref_12","first-page":"52","article-title":"Health risk assessment of adults consuming commercial fish contaminated with formaldehyde","volume":"3","author":"Abdullah","year":"2013","journal-title":"Food Public Health"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1016\/j.foodchem.2018.01.021","article-title":"A biodegradable colorimetric film for rapid low-cost field determination of formaldehyde contamination by digital image colorimetry","volume":"249","author":"Wongniramaikul","year":"2018","journal-title":"Food Chem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"100310","DOI":"10.1016\/j.sbsr.2019.100310","article-title":"Development of biodegradable hybrid polymer film for detection of formaldehyde in seafood products","volume":"27","author":"Rovina","year":"2020","journal-title":"Sens. Bio-Sens. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"3623","DOI":"10.1016\/j.snb.2017.09.080","article-title":"Rapid microfluidic paper-based platform for low concentration formaldehyde detection","volume":"255","author":"Guzman","year":"2018","journal-title":"Sens. Actuators B."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1016\/j.cej.2017.09.128","article-title":"Microfluidic paper-based chip platform for formaldehyde concentration detection","volume":"332","author":"Liu","year":"2018","journal-title":"Chem. Eng. J."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"280","DOI":"10.3358\/shokueishi.3.280","article-title":"Determination of formaldehyde in food (I)","volume":"3","author":"Sawamura","year":"1962","journal-title":"Shokuhin Eiseigaku Zasshi (Food Hyg. Saf. Sci.)"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1111\/j.1365-2621.1993.tb01274.x","article-title":"Formaldehyde instead of dimethylamine determination as a measure of total formaldehyde formed in frozen Argentine hake (Merluccius hubbsi)","volume":"28","author":"Boeri","year":"1993","journal-title":"Int. J. Food Sci. Technol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1097\/DER.0000000000000478","article-title":"Formaldehyde release from baby wipes: Analysis using the chromotropic acid method","volume":"30","author":"Liou","year":"2019","journal-title":"Dermatitis"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.aca.2019.03.063","article-title":"Rapid screening of formaldehyde in food using paper-based titration","volume":"1069","author":"Taarab","year":"2019","journal-title":"Anal. Chim. Acta."},{"key":"ref_21","unstructured":"Walker, J.F. (1944). Formaldehyde, Reinhold Publishing Corporation. [1st ed.]."},{"key":"ref_22","unstructured":"US Department of Health and Human Services (2017). NIOSH Manual of Analytical Methods (NMAM)."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1016\/j.talanta.2015.06.012","article-title":"Thin films exhibiting multicolor changes induced by formaldehyde-responsive release of anionic dyes","volume":"144","author":"Denda","year":"2015","journal-title":"Talanta"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.tifs.2021.08.029","article-title":"Paper-based microfluidics for food safety and quality analysis","volume":"118","author":"Nilghaz","year":"2021","journal-title":"Trends Food Sci. Technol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1627","DOI":"10.1111\/1541-4337.12914","article-title":"Multiplex optical bioassays for food safety analysis: Toward on-site detection","volume":"21","author":"Guan","year":"2022","journal-title":"Compr. Rev. Food Sci Food Saf."},{"key":"ref_26","unstructured":"Hermanson, G.T. (2008). Bioconjugate Techniques, Academic Press. [2nd ed.]."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.2134\/jeq2013.08.0336","article-title":"Evaluation and ap plication of a paper-based device for the determination of reactive phosphate in soil solution","volume":"43","author":"Jayawardane","year":"2014","journal-title":"J. Environ. Qual."},{"key":"ref_28","unstructured":"Miller, J.N., and Miller, J.C. (2010). Statistics and Chemometrics for Analytical Chemistry, Pearson Education Limited. [6th ed.]."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"43076","DOI":"10.1002\/app.43076","article-title":"Influence of chloroacetate group on physical properties of natural rubber and its interaction with fillers","volume":"133","author":"Boochathum","year":"2016","journal-title":"J. Appl. Polym. Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"107956","DOI":"10.1016\/j.foodcont.2021.107956","article-title":"Smartphone-based digital images as a novel approach to determine formaldehyde as a milk adulterant","volume":"125","author":"Silva","year":"2021","journal-title":"Food Control"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"104178","DOI":"10.1016\/j.jfca.2021.104178","article-title":"Novel approach for screening milk based on fast and environmentally friendly determination of protein and fat","volume":"104","author":"Silva","year":"2021","journal-title":"J. Food Compost. 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