{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T21:05:30Z","timestamp":1769115930074,"version":"3.49.0"},"reference-count":27,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,3,28]],"date-time":"2022-03-28T00:00:00Z","timestamp":1648425600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Health, Malaysia","award":["JPP-IMR 16-031"],"award-info":[{"award-number":["JPP-IMR 16-031"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Dioscorea hispida Dennst. locally known as \u201cubi gadung\u201d has been used as a traditional remedy and source of carbohydrate among Malaysians. To assess the effect of Dioscorea hispida aqueous extract (DHAE) on the production of reactive oxygen species (ROS) and their effects on DNA damage in Sprague Dawley rat\u2019s placental tissues, pregnant rats were randomly divided into four groups. The animals were orally treated with distilled water (negative control) and three different concentrations of DHAE (250, 500 and 1000 mg\/kg body weight (BW)) from gestation day 6 until 20. The oxidative stress in placental tissues was evaluated at day 21 by measuring the level of ROS, superoxide dismutase (SOD) and lipid peroxidation biomarker, malondialdehyde (MDA) while comet assay was used for DNA damage. There was no significant production of ROS and SOD activities in all groups. Significant changes were observed in the MDA level at 1000 mg\/kg BW DHAE. Comet assay revealed a significant increase (p &lt; 0.05) of DNA damage on animals treated with 250 and 500 mg\/kg BW DHAE but not at the highest concentration. It was postulated that the placental cells could have undergone necrosis which destroys all components including DNA. This occurrence simultaneously reduces the levels of DNA damage which can be represented by lower level of tail moments. This finding correlates with our histopathological examination where necrotic cells of spongiotrophoblast were observed in the basal zone of placental tissue. The high amount of hydrogen cyanide and other compounds in 1000 mg\/kg BW DHAE could elevate the lipid peroxidation and directly induce cell necrosis which requires further investigation.<\/jats:p>","DOI":"10.3390\/molecules27072190","type":"journal-article","created":{"date-parts":[[2022,3,29]],"date-time":"2022-03-29T21:41:30Z","timestamp":1648590090000},"page":"2190","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Oxidative Stress and DNA Damage Effect of Dioscorea hispida Dennst. on Placental Tissues of Rats"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4892-6219","authenticated-orcid":false,"given":"Hussin","family":"Muhammad","sequence":"first","affiliation":[{"name":"Herbal Medicine Research Centre, Institute for Medical Research, National Institutes of Health, Jalan Setia Murni U13\/52, Seksyen U13, Setia Alam, Shah Alam 40170, Selangor, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tengku Aideed Syah Tg Abu","family":"Bakar","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Technology, Faculty of Health Sciences, Universiti Teknologi MARA UiTM Puncak Alam, Bandar Puncak Alam 42300, Selangor, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhamad Faizul Adhzim","family":"Yusery","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Technology, Faculty of Health Sciences, Universiti Teknologi MARA UiTM Puncak Alam, Bandar Puncak Alam 42300, Selangor, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norizah","family":"Awang","sequence":"additional","affiliation":[{"name":"Herbal Medicine Research Centre, Institute for Medical Research, National Institutes of Health, Jalan Setia Murni U13\/52, Seksyen U13, Setia Alam, Shah Alam 40170, Selangor, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wan Mazlina Md.","family":"Saad","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Technology, Faculty of Health Sciences, Universiti Teknologi MARA UiTM Puncak Alam, Bandar Puncak Alam 42300, Selangor, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3570-1208","authenticated-orcid":false,"given":"Elda Nurafnie","family":"Ibnu Rasid","sequence":"additional","affiliation":[{"name":"Herbal Medicine Research Centre, Institute for Medical Research, National Institutes of Health, Jalan Setia Murni U13\/52, Seksyen U13, Setia Alam, Shah Alam 40170, Selangor, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3962-8666","authenticated-orcid":false,"given":"Mohamad Fawzi","family":"Mahomoodally","sequence":"additional","affiliation":[{"name":"Department of Health Sciences, Faculty of Medicine and Health Science, University of Mauritius, R\u00e9duit 80837, Mauritius"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maizatul Hasyima","family":"Omar","sequence":"additional","affiliation":[{"name":"Herbal Medicine Research Centre, Institute for Medical Research, National Institutes of Health, Jalan Setia Murni U13\/52, Seksyen U13, Setia Alam, Shah Alam 40170, Selangor, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"7404082","DOI":"10.1155\/2018\/7404082","article-title":"The Role of Oxidative Stress in the Pathomechanism of Congenital Malformations","volume":"2018","author":"Laforgia","year":"2018","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1097\/WOX.0b013e3182439613","article-title":"Oxidative Stress and Antioxidant Defense","volume":"5","author":"Birben","year":"2012","journal-title":"World Allergy Organ. J."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1080\/10715760802510069","article-title":"Oxidative Stress Early in Pregnancy and Pregnancy Outcome","volume":"42","author":"Stein","year":"2008","journal-title":"Free Radic. Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1080\/jmf.10.3.182.185-10","article-title":"Oxidant Damage to DNA and Pregnancy Outcome","volume":"10","author":"Scholl","year":"2001","journal-title":"J. Matern. Fetal Med."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"37","DOI":"10.37757\/MR2013V15.N1.9","article-title":"Oxidative Stress and Birth Defects in Infants of Women with Pregestational Diabetes","volume":"15","year":"2013","journal-title":"MEDICC Rev."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1016\/j.placenta.2009.02.007","article-title":"Effect of Oxidative Stress on Birth Sizes: Consideration of Window from Mid Pregnancy to Delivery","volume":"30","author":"Min","year":"2009","journal-title":"Placenta"},{"key":"ref_7","first-page":"15","article-title":"Food and Medicinal Values of Certain Species of Dioscorea with Special Reference to Assam","volume":"3","author":"Dutta","year":"2015","journal-title":"J. Pharmacogn. Phytochem."},{"key":"ref_8","first-page":"33","article-title":"Anatomical Study of Stem, Petiole, Leaf, Tuber, Root and Flower of Dioscorea hispida Dennst. (Dioscoreaceae) by Using Optical Microscope, SEM and TEM","volume":"4","author":"Tajuddin","year":"2013","journal-title":"J. Agrobiotechnol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1177\/0883911515597704","article-title":"Novel Dioscorea hispida Starch-Based Hydrogels and Their Beneficial Use as Disinfectants","volume":"31","author":"Azman","year":"2016","journal-title":"J. Bioact. Compat. Polym."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1055\/s-0033-1360330","article-title":"Characterization of Steroidal Saponins from Dioscorea villosa and D. cayenensis Using Ultrahigh Performance Liquid Chromatography\/Electrospray Ionization Quadrupole Time-of-Flight Mass Spectrometry","volume":"80","author":"Avula","year":"2014","journal-title":"Planta Med."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1016\/j.whi.2015.03.001","article-title":"Women\u2019s Use and Self-Prescription of Herbal Medicine during Pregnancy: An Examination of 1835 Pregnant Women","volume":"25","author":"Frawley","year":"2015","journal-title":"Women\u2019s Health Issues"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1279","DOI":"10.1016\/j.fct.2012.02.027","article-title":"A 90-Day Subchronic Toxicological Assessment of Dioscin, a Natural Steroid Saponin, in Sprague-Dawley Rats","volume":"50","author":"Xu","year":"2012","journal-title":"Food Chem. Toxicol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1016\/j.jep.2009.08.047","article-title":"Acute Toxicity and Sub-Chronic Toxicity of Steroidal Saponins from Dioscorea zingiberensis C.H.Wright in Rodents","volume":"126","author":"Qin","year":"2009","journal-title":"J. Ethnopharmacol."},{"key":"ref_14","first-page":"496","article-title":"Potential Toxicities of Herbal Therapies in the Developing Fetus","volume":"68","author":"Jurgens","year":"2003","journal-title":"Dev. Reprod. Toxicol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"110538","DOI":"10.1016\/j.fct.2019.05.046","article-title":"Histopathological Changes in Placenta and Liver of Pregnant Rats Administered with Aqueous Extract of Dioscorea hispida var. daemona (Roxb)","volume":"131","author":"Muhammad","year":"2019","journal-title":"Food Chem. Toxicol."},{"key":"ref_16","first-page":"69","article-title":"Hydrogen Cyanide Content of Manihort utilissima, Colocasia esculenta, Dioscorea bulbifera and Dioscorea domentorum Tubers Found in Benue State","volume":"4","author":"Anhwange","year":"2011","journal-title":"Int. J. Chem."},{"key":"ref_17","first-page":"103","article-title":"Changes in the Levels of Lipoperoxide and Antioxidant Factors in Human Placenta during Gestation","volume":"44","author":"Takehara","year":"1990","journal-title":"Acta Med. Okayama"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1272","DOI":"10.1101\/gad.1199904","article-title":"Alkylating DNA Damage Stimulates a Regulated Form of Necrotic Cell Death","volume":"18","author":"Zong","year":"2004","journal-title":"Genes Dev."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"360438","DOI":"10.1155\/2014\/360438","article-title":"Lipid Peroxidation: Production, Metabolism, and Signaling Mechanisms of Malondialdehyde and 4-Hydroxy-2-Nonenal","volume":"2014","author":"Ayala","year":"2014","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/0165-1110(90)90014-3","article-title":"Possible Mutagens Derived from Lipids and Lipid Precursors","volume":"238","author":"Esterbauer","year":"1990","journal-title":"Mutat. Res. Genet. Toxicol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"822","DOI":"10.1021\/tx000222a","article-title":"Elucidation of Reaction Scheme Describing Malondialdehyde-Acetaldehyde-Protein Adduct Formation","volume":"14","author":"Tuma","year":"2001","journal-title":"Chem. Res. Toxicol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1016\/j.jpba.2010.05.019","article-title":"Characterization of Steroidal Saponins in Crude Extracts from Dioscorea zingiberensis C. H. Wright by Ultra-Performance Liquid Chromatography\/Electrospray Ionization Quadrupole Time-of-Flight Tandem Mass Spectrometry","volume":"53","author":"Zhu","year":"2010","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_23","first-page":"47","article-title":"Dioscin Prevents the Mitochondrial Apoptosis and Attenuates Oxidative Stress in Cardiac H9c2 Cells","volume":"64","author":"Qin","year":"2014","journal-title":"Drug Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"120","DOI":"10.3389\/fphar.2017.00120","article-title":"Protective Effects of Dioscin against Lipopolysaccharide-Induced Acute Lung Injury through Inhibition of Oxidative Stress and Inflammation","volume":"8","author":"Yao","year":"2017","journal-title":"Front. Pharmacol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.redox.2018.02.026","article-title":"Protective Effect of Dioscin against Doxorubicin-Induced Cardiotoxicity via Adjusting MicroRNA-140-5p-Mediated Myocardial Oxidative Stress","volume":"16","author":"Zhao","year":"2018","journal-title":"Redox Biol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1396","DOI":"10.1104\/pp.112.200345","article-title":"Extending Cassava Root Shelf Life via Reduction of Reactive Oxygen Species Production","volume":"159","author":"Zidenga","year":"2012","journal-title":"Plant Physiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"144","DOI":"10.5344\/ajev.1965.16.3.144","article-title":"Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents","volume":"16","author":"Singleton","year":"1965","journal-title":"Am. J. Enol. Vitic."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/7\/2190\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:45:03Z","timestamp":1760136303000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/7\/2190"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,28]]},"references-count":27,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2022,4]]}},"alternative-id":["molecules27072190"],"URL":"https:\/\/doi.org\/10.3390\/molecules27072190","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3,28]]}}}