{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T04:26:09Z","timestamp":1760502369844,"version":"build-2065373602"},"reference-count":44,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,4,3]],"date-time":"2022-04-03T00:00:00Z","timestamp":1648944000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Oriental Medicine Research Foundation","award":["30952128"],"award-info":[{"award-number":["30952128"]}]},{"name":"Ministry of Education, Culture, Sports and Technology of Japan","award":["#21K08427"],"award-info":[{"award-number":["#21K08427"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Callerya speciosa is widely distributed in tropical and subtropical countries and is traditionally used for preventing numerous disorders. In this study, a bioguided fractionation of ethyl acetate extract (SE) from C. speciosa root was carried out to target antioxidant and cytotoxic activities. Of the four fractions (SE1-SE4) obtained by column chromatography, SE4 had the strongest anti-radical ability in the DPPH and ABTS assays (IC50 = 0.05 and 0.17 mg\/mL, respectively), with results close to butylated hydroxytoluene (BHT), a common antioxidant agent. The cytotoxic activities against the selected cells were analyzed in this study by MTT assay. Accordingly, SE2, SE3, and SE4 significantly inhibited the viability of multiple myeloma cell lines, comprising U266 (IC50 = 0.38, 0.09, and 0.11 mg\/mL, respectively) and KMS11 (IC50 = 0.09, 0.17, and 0.15 mg\/mL, respectively), mantle cell lymphoma Mino (IC50 = 0.08, 0.16, and 0.15 mg\/mL, respectively), and the noncancerous cell line LCL (IC50 = 0.40, 0.32, and 0.21 mg\/mL, respectively). At a concentration of 125 \u00b5g\/mL, SE2, SE3, and SE4 induced the cell apoptosis of U266 (32.2%, 53.2%, and 55.6%, respectively), KMS11 (36.9%, 40.8%, and 47.9%, respectively), Mino (36.6%, 39.8%, and 22.0%, respectively), and LCL (12.4%, 17.5%, and 23.5%, respectively) via annexin V assay. The dominant compounds detected in fractions by high-performance liquid chromatography\u2013electrospray ionization\u2013tandem mass spectrometry (HPLC-ESI-MS\/MS), were identified as isoflavones. This is the first report describing C. speciosa as a promising natural source of antileukemia and antimyeloma agents, which may be useful for the development of blood cancer treatments.<\/jats:p>","DOI":"10.3390\/molecules27072322","type":"journal-article","created":{"date-parts":[[2022,4,4]],"date-time":"2022-04-04T05:50:43Z","timestamp":1649051443000},"page":"2322","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Cytotoxicity of Callerya speciosa Fractions against Myeloma and Lymphoma Cell Lines"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5440-1120","authenticated-orcid":false,"given":"Vu Quang","family":"Lam","sequence":"first","affiliation":[{"name":"Division of Hematology, Department of Internal Medicine, Aichi Medical University School of Medicine, Nagakute 480-1195, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6844-8296","authenticated-orcid":false,"given":"La Hoang","family":"Anh","sequence":"additional","affiliation":[{"name":"Transdisciplinary Science and Engineering Program, Graduate School of Advanced Science and Engineering, Hiroshima University, Hiroshima 739-8529, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6567-9902","authenticated-orcid":false,"given":"Nguyen Van","family":"Quan","sequence":"additional","affiliation":[{"name":"Transdisciplinary Science and Engineering Program, Graduate School of Advanced Science and Engineering, Hiroshima University, Hiroshima 739-8529, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7103-5698","authenticated-orcid":false,"given":"Tran Dang","family":"Xuan","sequence":"additional","affiliation":[{"name":"Transdisciplinary Science and Engineering Program, Graduate School of Advanced Science and Engineering, Hiroshima University, Hiroshima 739-8529, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6681-8927","authenticated-orcid":false,"given":"Ichiro","family":"Hanamura","sequence":"additional","affiliation":[{"name":"Division of Hematology, Department of Internal Medicine, Aichi Medical University School of Medicine, Nagakute 480-1195, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2983-2129","authenticated-orcid":false,"given":"Kaori","family":"Uchino","sequence":"additional","affiliation":[{"name":"Division of Hematology, Department of Internal Medicine, Aichi Medical University School of Medicine, Nagakute 480-1195, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sivasundaram","family":"Karnan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Aichi Medical University, Nagakute 480-1195, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1822-9976","authenticated-orcid":false,"given":"Akiyoshi","family":"Takami","sequence":"additional","affiliation":[{"name":"Division of Hematology, Department of Internal Medicine, Aichi Medical University School of Medicine, Nagakute 480-1195, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"394","DOI":"10.3322\/caac.21492","article-title":"Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries","volume":"68","author":"Bray","year":"2018","journal-title":"CA Cancer J. Clin."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"209","DOI":"10.3322\/caac.21660","article-title":"Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries","volume":"71","author":"Sung","year":"2021","journal-title":"CA Cancer J. Clin."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Hanamura, I. (2021). Gain\/amplification of chromosome Arm 1q21 in multiple myeloma. Cancers, 13.","DOI":"10.3390\/cancers13020256"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1200\/JCO.2015.63.5904","article-title":"Mantle cell lymphoma","volume":"34","author":"Cheah","year":"2016","journal-title":"J. Clin. Oncol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/bs.podrm.2020.07.005","article-title":"Validation of in-vitro bioassay methods: Application in herbal drug research","volume":"Volume 46","year":"2021","journal-title":"Profiles of Drug Substances, Excipients and Related Methodology"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1038\/s41420-021-00446-8","article-title":"CD52 is a novel target for the treatment of FLT3-ITD-mutated myeloid leukemia","volume":"7","author":"Karnan","year":"2021","journal-title":"Cell Death Discov."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2012\/853175","article-title":"Oxidative stress and inflammation: Essential partners in alcoholic liver disease","volume":"2012","author":"Ambade","year":"2012","journal-title":"Int. J. Hepatol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1097\/FPC.0b013e32833ffb56","article-title":"Doxorubicin pathways: Pharmacodynamics and adverse effects","volume":"21","author":"Thorn","year":"2011","journal-title":"Pharmacogenet. Genom."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Xu, D.P., Li, Y., Meng, X., Zhou, T., Zhou, Y., Zheng, J., Zhang, J.J., and Li, H.B. (2017). Natural antioxidants in foods and medicinal plants: Extraction, assessment and resources. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18010096"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"4","DOI":"10.12970\/2308-8044.2020.08.02","article-title":"Active nature based ingredients for drug discovery with pivotal role of clinical efficacy: Review and prospective","volume":"8","author":"Teschke","year":"2020","journal-title":"J. Mod. Med. Chem."},{"key":"ref_11","first-page":"332","article-title":"Chemical constituents of Millettia speciosa","volume":"6","author":"Ding","year":"2014","journal-title":"Chin. Herb. Med."},{"key":"ref_12","first-page":"385","article-title":"Chemical constituents from roots of Millettia speciosa","volume":"8","author":"Fu","year":"2016","journal-title":"Chin. Herb. Med."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1016\/j.ijbiomac.2019.12.166","article-title":"A novel polysaccharide from the roots of Millettia speciosa Champ.: Preparation, structural characterization and immunomodulatory activity","volume":"145","author":"Huang","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"3391","DOI":"10.1002\/elps.200800174","article-title":"Interactions between thrombin and natural products of Millettia speciosa Champ. using capillary zone electrophoresis","volume":"29","author":"Zhang","year":"2008","journal-title":"Electrophoresis"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"479","DOI":"10.4314\/tjpr.v14i3.17","article-title":"Antifatigue effect of Millettiae speciosae Champ. (leguminosae) extract in mice","volume":"14","author":"Zhao","year":"2015","journal-title":"Trop. J. Pharm. Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1686","DOI":"10.1016\/j.jsps.2020.10.015","article-title":"A review on genus Millettia: Traditional uses, phytochemicals and pharmacological activities","volume":"28","author":"Jena","year":"2020","journal-title":"Saudi Pharm. J."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"580","DOI":"10.2174\/1573412914666180608095922","article-title":"Characterization and identification of isoflavonoids in the roots of Millettia speciosa Champ. by UPLC-Q-TOF-MS\/MS","volume":"15","author":"Yu","year":"2019","journal-title":"Curr. Pharm. Anal."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Ayesh, B.M., Abed, A.A., and Faris, D.M. (2014). In vitro inhibition of human leukemia THP-1 cells by Origanum syriacum L. and Thymus vulgaris L. extracts. BMC Res. Notes, 7.","DOI":"10.1186\/1756-0500-7-612"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1186\/1749-8546-7-15","article-title":"Evaluation of the anticancer potential of six herbs against a hepatoma cell line","volume":"7","author":"Weerapreeyakul","year":"2012","journal-title":"Chin. Med."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Pfeffer, C., and Singh, A. (2018). Apoptosis: A target for anticancer therapy. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19020448"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.taap.2008.06.004","article-title":"Progeric effects of catalase inactivation in human cells","volume":"232","author":"Koepke","year":"2008","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1603","DOI":"10.1016\/j.freeradbiomed.2010.09.006","article-title":"Oxidative stress, inflammation, and cancer: How are they linked?","volume":"49","author":"Reuter","year":"2010","journal-title":"Free Radic. Biol. Med."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/j.foodchem.2004.02.003","article-title":"Antioxidant activity and phenolic content of selected fruit seeds","volume":"88","author":"Soong","year":"2004","journal-title":"Food Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"605","DOI":"10.3390\/molecules24030605","article-title":"Antioxidant, \u03b1-amylase and \u03b1-glucosidase inhibitory activities and potential constituents of Canarium tramdenum Bark","volume":"24","author":"Quan","year":"2019","journal-title":"Molecules"},{"key":"ref_25","first-page":"142","article-title":"Antifungal and antioxidative potential of oil and extracts derived from leaves of Indian spice plant Cinnamomum tamala","volume":"58","author":"Pandey","year":"2012","journal-title":"Cell. Mol. Biol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1021\/np9904509","article-title":"Flavonoids as antioxidants","volume":"63","author":"Pietta","year":"2000","journal-title":"J. Nat. Prod."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Chaouhan, H.S., Li, X., Sun, K.-T., Wang, I.-K., Yu, T.-M., Yu, S.-H., Chen, K.-B., Lin, W.-Y., and Li, C.-Y. (2022). Calycosin alleviates paraquat-induced neurodegeneration by improving mitochondrial functions and regulating autophagy in a drosophila model of Parkinson\u2019s disease. Antioxidants, 11.","DOI":"10.3390\/antiox11020222"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"13914","DOI":"10.1038\/srep13914","article-title":"Structural characterization and evaluation of the antioxidant activity of phenolic compounds from Astragalus taipaishanensis and their structure-activity relationship","volume":"5","author":"Pu","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"355","DOI":"10.4103\/1735-5362.192484","article-title":"Pharmacological characterization of liquiritigenin, a chiral flavonoid in licorice","volume":"11","author":"Alrushaid","year":"2016","journal-title":"Res. Pharm. Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"69","DOI":"10.3390\/plants10010069","article-title":"Antioxidant, anti-tyrosinase, anti-\u03b1-amylase, and cytotoxic potentials of the invasive weed Andropogon virginicus","volume":"10","author":"Anh","year":"2020","journal-title":"Plants"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Anh, L.H., Xuan, T.D., Thuy, N.T.D., Quan, N.V., and Trang, L.T. (2020). Antioxidant and \u03b1-amylase inhibitory activities and phytocompounds of Clausena indica Fruits. Medicines, 7.","DOI":"10.3390\/medicines7030010"},{"key":"ref_32","unstructured":"Watson, R.R., Preedy, V.R., and Zibadi, S. (2014). Colorectal cancer prevention by wheat consumption. Wheat and Rice in Disease Prevention and Health, Academic Press."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.3109\/13880209.2013.879188","article-title":"Pharmaceutical properties of calycosin, the major bioactive isoflavonoid in the dry root extract of Radix astragali","volume":"52","author":"Gao","year":"2014","journal-title":"Pharm. Biol."},{"key":"ref_34","first-page":"1","article-title":"Ergosterol peroxide: An effect-directed detecting method from Anoectochilus elwesii and evaluation of anticancer activity","volume":"13","author":"Lin","year":"2021","journal-title":"Nat. Prod. Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"17956","DOI":"10.1038\/s41598-018-36411-2","article-title":"Ergosterol peroxide from marine fungus Phoma sp. induces ROS-dependent apoptosis and autophagy in human lung adenocarcinoma cells","volume":"8","author":"Wu","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"L\u00f3pez-Biedma, A., S\u00e1nchez-Quesada, C., Beltr\u00e1n, G., Delgado-Rodr\u00edguez, M., and Gaforio, J.J. (2016). Phytoestrogen (+)-pinoresinol exerts cytotoxic activity in breast cancer cells with different oestrogen receptor statuses. BMC Complement. Altern. Med., 16.","DOI":"10.1186\/s12906-016-1233-7"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1093\/carcin\/bgm255","article-title":"Chemopreventive properties of pinoresinol-rich olive oil involve a selective activation of the ATM-p53 cascade in colon cancer cell lines","volume":"29","author":"Fini","year":"2008","journal-title":"Carcinogenesis"},{"key":"ref_38","first-page":"1183","article-title":"Induction of apoptosis by maackiain and trifolirhizin (maackiain glycoside) isolated from sanzukon (Sophora Subprostrate Chen et T. Chen) in human promyelotic leukemia HL-60 cells","volume":"12","author":"Aratanechemuge","year":"2004","journal-title":"Oncol. Rep."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"e1465","DOI":"10.1038\/cddis.2014.429","article-title":"Medicarpin, a legume phytoalexin sensitizes myeloid leukemia cells to TRAIL-induced apoptosis through the induction of DR5 and activation of the ROS-JNK-CHOP pathway","volume":"5","author":"Trivedi","year":"2014","journal-title":"Cell Death Dis."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Justino, G.C. (2017). Flavonoids: Anticancer properties. Flavonoids: From Biosynthesis to Human Health, InTech.","DOI":"10.5772\/65575"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1111\/j.1742-7843.2009.00471.x","article-title":"Betulin elicits anti-cancer effects in tumour primary cultures and cell lines in vitro","volume":"105","author":"Rzeski","year":"2009","journal-title":"Basic Clin. Pharmacol. Toxicol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1016\/j.foodchem.2008.10.019","article-title":"Availability of polyphenols in fruit beverages subjected to in vitro gastrointestinal digestion and their effects on proliferation, cell-cycle and apoptosis in human colon cancer Caco-2 cells","volume":"114","author":"Cilla","year":"2009","journal-title":"Food Chem."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Quan, N.V., Xuan, T.D., and Teschke, R. (2020). Potential hepatotoxins found in herbal medicinal products: A systematic review. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21145011"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Quan, N.V., Xuan, T.D., Anh, L.H., and Tran, H.-D. (2019). Bio-guided isolation of prospective bioactive constituents from roots of Clausena indica (Dalzell) Oliv. Molecules, 24.","DOI":"10.3390\/molecules24244442"}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/7\/2322\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:49:28Z","timestamp":1760136568000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/7\/2322"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,3]]},"references-count":44,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2022,4]]}},"alternative-id":["molecules27072322"],"URL":"https:\/\/doi.org\/10.3390\/molecules27072322","relation":{},"ISSN":["1420-3049"],"issn-type":[{"type":"electronic","value":"1420-3049"}],"subject":[],"published":{"date-parts":[[2022,4,3]]}}}