{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T20:35:39Z","timestamp":1786480539944,"version":"build-2736575974"},"reference-count":36,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2022,4,23]],"date-time":"2022-04-23T00:00:00Z","timestamp":1650672000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science and Technology (Taiwan)","award":["107-2320-B-038-019."],"award-info":[{"award-number":["107-2320-B-038-019."]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Pinus taiwanensis Hayata (Pinaceae) is an endemic plant in Taiwan. According to the Chinese Materia Medica Grand Dictionary, the Pinus species is mainly used to relieve pain, and eliminate pus and toxicity. In this study, nineteen compounds were isolated from the ethyl acetate layer of the ethanolic extract of P. taiwanensis Hayata twigs using bioassay-guided fractionation, and their anti-melanoma effects were investigated through a B16-F10 mouse melanoma cell model. The structures of the purified compounds were identified by 2D-NMR, MS, and IR, including 1 triterpenoid, 9 diterpenoids, 2 lignans, 4 phenolics, 1 phenylpropanoid, 1 flavonoid, and 1 steroid. Among them, compound 3 was found to be a new diterpene. Some of the compounds (2, 5, 6, 17, 18) showed moderate cytotoxicity effects. On the other hand, the anti-melanoma effect was no better than that from the original ethyl acetate layer. We presumed it resulted from the synergistic effect, although further experimentation needs to be performed.<\/jats:p>","DOI":"10.3390\/molecules27092731","type":"journal-article","created":{"date-parts":[[2022,4,23]],"date-time":"2022-04-23T21:39:45Z","timestamp":1650749985000},"page":"2731","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Cytotoxicity Effect of Constituents of Pinus taiwanensis Hayata Twigs on B16-F10 Melanoma Cells"],"prefix":"10.3390","volume":"27","author":[{"given":"Man-Hsiu","family":"Chu","sequence":"first","affiliation":[{"name":"Graduate Institute of Pharmacognosy, Taipei Medical University, 250 Wu Xin Street, Taipei 11031, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sui-Wen","family":"Hsiao","sequence":"additional","affiliation":[{"name":"Ph.D. Program in Drug Discovery and Development Industry, College of Pharmacy, Taipei Medical University, 250 Wu Xin Street, Taipei 110301, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Chen","family":"Kao","sequence":"additional","affiliation":[{"name":"Graduate Institute of Pharmacy, Taipei Medical University, 250 Wu Xin Street, Taipei 11031, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hwa-Wen","family":"Yin","sequence":"additional","affiliation":[{"name":"Forestry Research Institute, Council of Agriculture, Executive Yuan, 53, Nanhai Road, Taipei 10066, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5935-6755","authenticated-orcid":false,"given":"Yueh-Hsiung","family":"Kuo","sequence":"additional","affiliation":[{"name":"Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Pharmacy, China Medical University, Taichung 40402, Taiwan"},{"name":"Department of Biotechnology, Asia University, Taichung 41354, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ching-Kuo","family":"Lee","sequence":"additional","affiliation":[{"name":"Graduate Institute of Pharmacognosy, Taipei Medical University, 250 Wu Xin Street, Taipei 11031, Taiwan"},{"name":"Ph.D. Program in Drug Discovery and Development Industry, College of Pharmacy, Taipei Medical University, 250 Wu Xin Street, Taipei 110301, Taiwan"},{"name":"Graduate Institute of Pharmacy, Taipei Medical University, 250 Wu Xin Street, Taipei 11031, Taiwan"},{"name":"Department of Chemistry, Chung Yuan Christian University, Zhongbei Road, Zhongli District, Taoyuan 32023, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,23]]},"reference":[{"key":"ref_1","unstructured":"(2005, November 12). 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