{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T17:16:50Z","timestamp":1787678210938,"version":"build-2784847793"},"reference-count":51,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2022,4,26]],"date-time":"2022-04-26T00:00:00Z","timestamp":1650931200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"UAE University","award":["21M129"],"award-info":[{"award-number":["21M129"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Inflammatory bowel diseases (IBDs) such as ulcerative colitis (UC) and Crohn\u2019s disease (CD) are diseases of the gastrointestinal system involving genetic and environmental factors attributed to oxidative stress and inflammation. Targeting oxidative stress and inflammation by novel dietary compounds of natural origin convincingly appears to be one of the important therapeutic strategies to keep the disease in remission. As there is no permanent cure for IBD except for chronic long-term treatment or surgery, it is therefore imperative to investigate plant-based agents that are receiving attention for their therapeutic benefits to overcome the debilitating clinical conditions of IBD. Lycopodium (LYCO), a plant of tropical and subtropical origin and known by numerous names such as ground pine, club moss, or devil\u2019s claw, has been popularly used for centuries in traditional medicine including Chinese and Indian medicines. In the present study, the effect of LYCO has been investigated in an acetic acid (AA)-induced colitis model in Wistar rats. LYCO was orally administered at the dose of 50 mg\/kg\/day either 3 days before or 30 min after the induction of IBD and continued for 7 days by intrarectal administration of AA. The changes in body weight and macroscopic and microscopic analysis of the colon of rats of different experimental groups were observed on days 0, 2, 4, and 7. The levels of myeloperoxidase (MPO), reduced glutathione (GSH), and malondialdehyde (MDA) were measured. AA caused a significant reduction in body weight and increased macroscopic and microscopic ulcer scores along with a significant decline in antioxidant enzymes, superoxide dismutase (SOD), and catalase and antioxidant substrate, glutathione (GSH). There was a concomitant increased formation of malondialdehyde (MDA), a marker of lipid peroxidation, and raised myeloperoxidase (MPO) activity, a marker of neutrophil activation. Treatment with LYCO significantly improved IBD-induced reduction in body weight, improved histology, inhibited MDA formation, and restored antioxidants along with reduced MPO activity. AA also caused the release of proinflammatory cytokines such as interleukin-1\u03b2 (IL-1\u03b2) and interleukin-23 (IL-23). Furthermore, AA also increased the levels of calprotectin, a protein released by neutrophils under inflammatory conditions of the gastrointestinal tract. LYCO treatment significantly reduced the release of calprotectin and proinflammatory cytokines. The results demonstrate that LYCO treatment has the potential to improve disease activity by inhibiting oxidative stress, lipid peroxidation, and inflammation along with histological preservation of colonic tissues.<\/jats:p>","DOI":"10.3390\/molecules27092774","type":"journal-article","created":{"date-parts":[[2022,4,26]],"date-time":"2022-04-26T21:37:53Z","timestamp":1651009073000},"page":"2774","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Lycopodium Mitigates Oxidative Stress and Inflammation in the Colonic Mucosa of Acetic Acid-Induced Colitis in Rats"],"prefix":"10.3390","volume":"27","author":[{"given":"Salim M. A.","family":"Bastaki","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Therapeutics, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain P.O. Box 15551, United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2141-3210","authenticated-orcid":false,"given":"Naheed","family":"Amir","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Therapeutics, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain P.O. Box 15551, United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1439-1562","authenticated-orcid":false,"given":"Ernest","family":"Adeghate","sequence":"additional","affiliation":[{"name":"Department of Anatomy, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain P.O. Box 15551, United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7801-2966","authenticated-orcid":false,"given":"Shreesh","family":"Ojha","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Therapeutics, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain P.O. Box 15551, United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1155\/2015\/136960","article-title":"Characterization of Inflammatory Bowel Disease in Elderly Patients: A Review of Epidemiology, Current Practices and Outcomes of Current Management Strategies","volume":"29","author":"Stepaniuk","year":"2015","journal-title":"Can. J. Gastroenterol. Hepatol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1627","DOI":"10.1016\/S0140-6736(07)60750-8","article-title":"Inflammatory bowel disease: Cause and immunobiology","volume":"369","author":"Baumgart","year":"2007","journal-title":"Lancet"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.1016\/j.suc.2015.07.006","article-title":"Inflammatory Bowel Disease: Historical Perspective, Epidemiology, and Risk Factors","volume":"95","author":"Malik","year":"2015","journal-title":"Surg. Clin. N. Am."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/s12944-015-0165-4","article-title":"Inflammatory bowel disease as a disorder of an imbalance between pro- and anti-inflammatory molecules and deficiency of resolution bioactive lipids","volume":"15","author":"Das","year":"2016","journal-title":"Lipids Health Dis."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1258\/ebm.2011.011358","article-title":"Oxidative stress and redox signaling mechanisms of inflammatory bowel disease: Updated experimental and clinical evidence","volume":"237","author":"Zhu","year":"2012","journal-title":"Exp. Biol. Med."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"3","DOI":"10.4103\/1319-3767.173753","article-title":"The implications of oxidative stress and antioxidant therapies in Inflammatory Bowel Disease: Clinical aspects and animal models","volume":"22","author":"Balmus","year":"2016","journal-title":"Saudi J. Gastroenterol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1152\/physrev.00040.2012","article-title":"Oxidative Stress: An Essential Factor in the Pathogenesis of Gastrointestinal Mucosal Diseases","volume":"94","author":"Bhattacharyya","year":"2014","journal-title":"Physiol. Rev."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"126","DOI":"10.3109\/08923973.2014.998368","article-title":"Anti-inflammatory and immunomodulatory effects of Spirulina platensis in comparison to Dunaliella salina in acetic acid-induced rat experimental colitis","volume":"37","author":"Farouk","year":"2015","journal-title":"Immunopharmacol. Immunotoxicol."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Jia, L., Xue, K., Liu, J., Habotta, O.A., Hu, L., and Abdel Moneim, A.E. (2020). Anticolitic Effect of Berberine in Rat Experimental Model: Impact of PGE2\/p38 MAPK Pathways. Mediat. Inflamm.","DOI":"10.1155\/2020\/9419085"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"493012","DOI":"10.1155\/2015\/493012","article-title":"Inflammatory Bowel Disease: An Overview of Immune Mechanisms and Biological Treatments","volume":"2015","author":"Garcia","year":"2015","journal-title":"Mediat. Inflamm"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.coph.2015.11.003","article-title":"Drug resistance in inflammatory bowel diseases","volume":"25","author":"Moreau","year":"2015","journal-title":"Curr. Opin. Pharmacol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"179616","DOI":"10.1155\/2015\/179616","article-title":"Botanical Drugs as an Emerging Strategy in Inflammatory Bowel Disease: A Review","volume":"2015","author":"Algieri","year":"2015","journal-title":"Mediat. Inflamm."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1053\/j.gastro.2016.10.004","article-title":"Complementary and Alternative Medicines Used by Patients With Inflammatory Bowel Diseases","volume":"152","author":"Cheifetz","year":"2017","journal-title":"Gastroenterology"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"42","DOI":"10.4103\/jrms.JRMS_590_17","article-title":"Phytotherapies in inflammatory bowel disease","volume":"24","year":"2019","journal-title":"J. Res. Med Sci."},{"key":"ref_15","first-page":"210","article-title":"Herbal and plant therapy in patients with inflammatory bowel disease","volume":"28","author":"Triantafyllidi","year":"2015","journal-title":"Ann. Gastroenterol."},{"key":"ref_16","first-page":"1883","article-title":"Complementary and Alternative Medicine Use Is Prevalent Among Patients with Gastrointestinal Diseases","volume":"60","author":"Hung","year":"2015","journal-title":"Am. J. Dig. Dis."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1055\/s-0043-104933","article-title":"Medicinal Plants in the Treatment of Colitis: Evidence from Preclinical Studies","volume":"83","author":"Santana","year":"2017","journal-title":"Planta Med."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1007\/s11010-005-9065-7","article-title":"Protective potentials of a potentized homeopathic drug, Lycopodium-30, in ameliorating azo dye induced hepatocarcinogenesis in mice","volume":"285","author":"Pathak","year":"2006","journal-title":"Mol. Cell. Biochem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1049\/iet-nbt.2017.0106","article-title":"Evaluation of potential anti-cancer activity of cationic liposomal nanoformulated","volume":"12","author":"Paramita","year":"2018","journal-title":"IET Nanobiotechnol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Jayaraj, R.L., Beiram, R., Azimullah, S., Meeran, M.F.N., Ojha, S.K., Adem, A., and Jalal, F.Y. (2019). Lycopodium Attenuates Loss of Dopaminergic Neurons by Suppressing Oxidative Stress and Neuroinflammation in a Rat Model of Parkinson\u2019s Disease. Molecules, 24.","DOI":"10.3390\/molecules24112182"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.jep.2011.10.042","article-title":"Investigation of the in vitro and ex vivo acetylcholinesterase and antioxidant activities of traditionally used Lycopodium species from South America on alkaloid extracts","volume":"139","author":"Konrath","year":"2012","journal-title":"J. Ethnopharmacol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.micpath.2017.09.026","article-title":"Beneficial immunomodulatory and neuro digestive effect in Trypanosoma cruzi infection after Lycopodium clavatum 13c treatment","volume":"112","author":"Ferraz","year":"2017","journal-title":"Microb. Pathog."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.jep.2006.07.018","article-title":"Appraisal of anti-inflammatory potential of the clubmoss, Lycopodium clavatum L.","volume":"109","author":"Orhan","year":"2007","journal-title":"J. Ethnopharmacol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1039\/b409720n","article-title":"The Lycopodium alkaloids","volume":"21","author":"Ma","year":"2004","journal-title":"Nat. Prod. Rep."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"103632","DOI":"10.1016\/j.bioorg.2020.103632","article-title":"Serratane triterpenoids isolated from Lycopodium clavatum by bioactivity-guided fractionation attenuate the production of inflammatory mediators","volume":"96","author":"Jo","year":"2020","journal-title":"Bioorganic Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1111\/j.1365-2036.1994.tb00343.x","article-title":"Another model of acetic acid-induced colitis in the rat","volume":"8","author":"Warren","year":"1994","journal-title":"Aliment. Pharmacol. Ther."},{"key":"ref_27","first-page":"G527","article-title":"An orally active inhibitor of leukotriene synthesis accelerates healing in a rat model of colitis","volume":"258","author":"Wallace","year":"1990","journal-title":"Am. J. Physiol. Content"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1159\/000245984","article-title":"Co-existence of Leukoderma With Features of Dowling-Degos Disease: Reticulate Acropigmentation of Kitamura Spectrum in Five Unrelated Patients","volume":"195","author":"Lestringant","year":"1997","journal-title":"Dermatology"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.1084\/jem.182.5.1281","article-title":"Antibodies to interleukin 12 abrogate established experimental colitis in mice","volume":"182","author":"Neurath","year":"1995","journal-title":"J. Exp. Med."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.fct.2018.03.002","article-title":"Ziziphus spina-christi fruit extract suppresses oxidative stress and p38 MAPK expression in ulcerative colitis in rats via induction of Nrf2 and HO-1 expression","volume":"115","author":"Almeer","year":"2018","journal-title":"Food Chem. Toxicol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1159\/000198104","article-title":"Experimental Production of Diffuse Colitis in Rats","volume":"17","author":"MacPherson","year":"1978","journal-title":"Digestion"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1756","DOI":"10.1053\/j.gastro.2011.02.016","article-title":"Proinflammatory cytokines in the pathogenesis of inflammatory bowel diseases","volume":"140","author":"Strober","year":"2011","journal-title":"Gastroenterology"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"5276130","DOI":"10.1155\/2016\/5276130","article-title":"Role of Antioxidants and Natural Products in Inflammation","volume":"2016","author":"Arulselvan","year":"2016","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_34","first-page":"1792","article-title":"Protective effect of Lagerstroemia speciosa against dextran sulfate sodium induced ulcerative colitis in C57BL\/6 mice","volume":"9","author":"Chaudhary","year":"2017","journal-title":"Am. J. Transl. Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1016\/j.jep.2016.06.016","article-title":"Mentha longifolia protects against acetic-acid induced colitis in rats","volume":"190","author":"Murad","year":"2016","journal-title":"J. Ethnopharmacol."},{"key":"ref_36","first-page":"205","article-title":"Effect of Coriandrum sativum hydroalcoholic extract and its essential oil on acetic acid- induced acute colitis in rats","volume":"6","author":"Heidari","year":"2016","journal-title":"Avicenna J. Phytomed."},{"key":"ref_37","first-page":"4210","article-title":"Menthol inhibits oxidative stress and inflammation in acetic acid-induced colitis in rat colonic mucosa","volume":"10","author":"Bastaki","year":"2018","journal-title":"Am. J. Transl. Res."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Bastaki, S.M.A., Al Ahmed, M.M., Al Zaabi, A., Amir, N., and Adeghate, E. (2016). Effect of turmeric on colon histology, body weight, ulcer, IL-23, MPO and glutathione in acetic-acid-induced inflammatory bowel disease in rats. BMC Complement. Altern. Med., 16.","DOI":"10.1186\/s12906-016-1057-5"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1159\/000171465","article-title":"The Role of Blood Flow in Gastric Mucosal Defence, Damage and Healing","volume":"12","author":"Svanes","year":"1994","journal-title":"Dig. Dis."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1016\/j.bpg.2010.08.008","article-title":"The integrity of the esophageal mucosa. Balance between offensive and defensive mechanisms","volume":"24","author":"Orlando","year":"2010","journal-title":"Best Pract. Res Clin Gastroenterol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1189\/jlb.1204697","article-title":"Myeloperoxidase: Friend and foe","volume":"77","author":"Klebanoff","year":"2005","journal-title":"J. Leukoc. Biol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1016\/S0009-9120(02)00361-2","article-title":"Oxidative stress and antioxidant capacity in patients with inflammatory bowel disease","volume":"35","author":"Erdil","year":"2002","journal-title":"Clin. Biochem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"187","DOI":"10.4103\/1319-3767.36750","article-title":"Lipid peroxides in patients with inflammatory bowel disease","volume":"13","author":"Alzoghaibi","year":"2007","journal-title":"Saudi. J. Gastroenterol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"4280","DOI":"10.3748\/wjg.14.4280","article-title":"Role of cytokines in inflammatory bowel disease","volume":"14","year":"2008","journal-title":"World J. Gastroenterol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1016\/0016-5085(94)90614-9","article-title":"Cytokines in intestinal inflammation: Pathophysiological and clinical considerations","volume":"106","author":"Sartor","year":"1994","journal-title":"Gastroenterology"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1344","DOI":"10.1016\/0016-5085(84)90202-6","article-title":"Quantitative assay for acute intestinal inflammation based on myeloperoxidase activity. Assessment of inflammation in rat and hamster models","volume":"87","author":"Krawisz","year":"1984","journal-title":"Gastroenterology"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/0160-5402(85)90029-4","article-title":"Myeloperoxidase activity as a quantitative assessment of neutrophil infiltration into ischemic myocardium","volume":"14","author":"Mullane","year":"1985","journal-title":"J. Pharm. Methods"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"403","DOI":"10.3892\/br.2016.751","article-title":"Calprotectin as a diagnostic tool for inflammatory bowel diseases","volume":"5","author":"Chatzikonstantinou","year":"2016","journal-title":"Biomed. Rep."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"104181","DOI":"10.1016\/j.fitote.2019.104181","article-title":"Serratene triterpenoids and their biological activities from Lycopodiaceae plants","volume":"136","author":"Thasana","year":"2019","journal-title":"Fitoterapia"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"351","DOI":"10.3389\/fphar.2019.00351","article-title":"Plant-Derived Alkaloids: The Promising Disease-Modifying Agents for Inflammatory Bowel Disease","volume":"10","author":"Peng","year":"2019","journal-title":"Front. Pharmacol."},{"key":"ref_51","first-page":"1","article-title":"Appendix to plants used by the Saraguros","volume":"1","author":"Ellemann","year":"1990","journal-title":"Bot. Inst. Aarhus Univ. Aarhus"}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/9\/2774\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:01:37Z","timestamp":1760137297000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/9\/2774"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,26]]},"references-count":51,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2022,5]]}},"alternative-id":["molecules27092774"],"URL":"https:\/\/doi.org\/10.3390\/molecules27092774","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,4,26]]}}}