{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T01:23:03Z","timestamp":1760232183509,"version":"build-2065373602"},"reference-count":38,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2022,4,27]],"date-time":"2022-04-27T00:00:00Z","timestamp":1651017600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Danish Council for Independent Research, Technology, and Production Sciences","award":["0602-01911B (11-107077)"],"award-info":[{"award-number":["0602-01911B (11-107077)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Background: [18F]FDG and [11C]methionine accumulate in lymph nodes draining S. aureus -infected foci. The lymph nodes were characterized by weight, [11C]methionine- and [18F]FDG-positron emissions tomography (PET)\/computed tomography (CT), and immunohistochemical (IHC)-staining. Methods: 20 pigs inoculated with S. aureus into the right femoral artery were PET\/CT-scanned with [18F]FDG, and nine of the pigs were additionally scanned with [11C]methionine. Mammary, medial iliac, and popliteal lymph nodes from the left and right hind limbs were weighed. IHC-staining for calculations of area fractions of Ki-67, L1, and IL-8 positive cells was done in mammary and popliteal lymph nodes from the nine pigs. Results: The pigs developed one to six osteomyelitis foci. Some pigs developed contiguous infections of peri-osseous tissue and inoculation-site abscesses. Weights of mammary and medial iliac lymph nodes and their [18F]FDG maximum Standardized Uptake Values (SUVFDGmax) showed a significant increase in the inoculated limb compared to the left limb. Popliteal lymph node weight and their FDG uptake did not differ significantly between hind limbs. Area fractions of Ki-67 and IL-8 in the right mammary lymph nodes and SUVMetmax in the right popliteal lymph nodes were significantly increased compared with the left side. Conclusion: The PET-tracers [18F]FDG and [11C]methionine, and the IHC- markers Ki-67 and IL-8, but not L1, showed increased values in lymph nodes draining soft tissues infected with S. aureus. The increase in [11C]methionine may indicate a more acute lymph node response, whereas an increase in [18F]FDG may indicate a more chronic response.<\/jats:p>","DOI":"10.3390\/molecules27092792","type":"journal-article","created":{"date-parts":[[2022,4,27]],"date-time":"2022-04-27T22:20:20Z","timestamp":1651098020000},"page":"2792","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Lymph Nodes Draining Infections Investigated by PET and Immunohistochemistry in a Juvenile Porcine Model"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1512-4981","authenticated-orcid":false,"given":"Pia","family":"Afzelius","sequence":"first","affiliation":[{"name":"Department of Clinical Physiology and Nuclear Medicine Zealand Koege, University Hospital of Copenhagen, 4600 Copenhagen, Denmark"},{"name":"Department of Nuclear Medicine, Aalborg University Hospital, 9000 Aalborg, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4819-7397","authenticated-orcid":false,"given":"Malene Kjelin","family":"Morsing","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences, University of Copenhagen, 1870 Frederiksberg C, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2678-6622","authenticated-orcid":false,"given":"Ole Lerberg","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences, University of Copenhagen, 1870 Frederiksberg C, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0084-9122","authenticated-orcid":false,"given":"Aage Kristian Olsen","family":"Alstrup","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine and PET, Aarhus University Hospital, Skejby, 8200 Aarhus, Denmark"},{"name":"Department of Clinical Medicine, Aarhus University, Skejby, 8200 Aarhus, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8575-3703","authenticated-orcid":false,"given":"Svend Borup","family":"Jensen","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine, Aalborg University Hospital, 9000 Aalborg, Denmark"},{"name":"Department of Chemistry and Biosciences, Aalborg University, 9220 Aalborg, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6811-0172","authenticated-orcid":false,"given":"Lars","family":"J\u00f8dal","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine, Aalborg University Hospital, 9000 Aalborg, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"623036","DOI":"10.1155\/2013\/623036","article-title":"The Use of 18F-FDG-PET\/CT for Diagnosis and Treatment Monitoring of Inflammatory and Infectious Diseases","volume":"2013","author":"Glaudemans","year":"2013","journal-title":"Clin. 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