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As \u03b2CDPEGs display different physicochemical properties than their precursors, they could also trigger distinct cellular responses. To investigate the biological behavior of \u03b2CDPEGs in comparison to their parent compounds, we performed broad toxicological assays on RAW 264.7 macrophages, MC3T3-E1 osteoblasts, and MDCK cells. By analyzing ROS and NO2\u2212 overproduction in macrophages, we found that \u03b2CDPEGs induced a moderate stress response without affecting cell viability. Although MC3T3-E1 osteoblasts were more sensitive than MDCK cells to \u03b2CDPEGs and the parent compounds, a similar pattern was observed: the effect of \u03b2CDPEG5 on cell viability and cell cycle progression was larger than that of \u03b2CDPEG2; PEG2 affected cell viability and cell cycle more than \u03b2CDPEG2; cell post-treatment recovery was favorable in all cases, and the compounds had similar behaviors regarding ROS generation. The effect on MDCK cell migration followed a similar pattern. In contrast, for osteoblasts, the interference of \u03b2CDPEG5 with cell migration was smaller than that of \u03b2CDPEG2; likewise, the effect of PEG2 was shorter than its conjugate. Overall, the covalent conjugation of \u03b2CD and PEGs, particularly to yield \u03b2CDPEG2, improved the biocompatibility profile, evidencing that a favorable biological response can be tuned through a thoughtful combination of materials. Moreover, this is the first time that an in vitro evaluation of \u03b2CD and PEG has been presented for MC3T3-E1 and MDCK cells, thus providing valuable knowledge for designing biocompatible nanomaterials constructed from \u03b2CD and PEGs.<\/jats:p>","DOI":"10.3390\/molecules27093026","type":"journal-article","created":{"date-parts":[[2022,5,8]],"date-time":"2022-05-08T21:31:20Z","timestamp":1652045480000},"page":"3026","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Exploring In Vitro Biological Cellular Responses of Pegylated \u03b2-Cyclodextrins"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5766-5631","authenticated-orcid":false,"given":"Juliana","family":"Rinc\u00f3n-L\u00f3pez","sequence":"first","affiliation":[{"name":"Laboratorio de Materiales Supramoleculares (SupraMatLab), Instituto de Investigaciones en Materiales, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Circuito Exterior S\/N, Ciudad Universitaria, Coyoac\u00e1n 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3570-6367","authenticated-orcid":false,"given":"Miguelina","family":"Mart\u00ednez-Aguilera","sequence":"additional","affiliation":[{"name":"Laboratorio de Materiales Supramoleculares (SupraMatLab), Instituto de Investigaciones en Materiales, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Circuito Exterior S\/N, Ciudad Universitaria, Coyoac\u00e1n 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Guadarrama","sequence":"additional","affiliation":[{"name":"Laboratorio de Materiales Supramoleculares (SupraMatLab), Instituto de Investigaciones en Materiales, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Circuito Exterior S\/N, Ciudad Universitaria, Coyoac\u00e1n 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6171-8601","authenticated-orcid":false,"given":"Karla","family":"Juarez-Moreno","sequence":"additional","affiliation":[{"name":"Centro de F\u00edsica Aplicada y Tecnolog\u00eda Avanzada, Universidad Nacional Aut\u00f3noma de M\u00e9xico, (CFATA-UNAM), Blvd. Juriquilla #3001 Col. Jurica La Mesa CP, Quer\u00e9taro 76230, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5650-8637","authenticated-orcid":false,"given":"Yareli","family":"Rojas-Aguirre","sequence":"additional","affiliation":[{"name":"Laboratorio de Materiales Supramoleculares (SupraMatLab), Instituto de Investigaciones en Materiales, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Circuito Exterior S\/N, Ciudad Universitaria, Coyoac\u00e1n 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Dodziuk, H. (2006). Molecules with Holes\u2014Cyclodextrins. Cyclodextrins and Their Complexes, Wiley-VCH Verlag GmbH & Co. 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