{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T12:06:53Z","timestamp":1787314013559,"version":"build-2736575974"},"reference-count":56,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2022,5,10]],"date-time":"2022-05-10T00:00:00Z","timestamp":1652140800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Universidad Central de Chile","award":["CIP2019015 (MAVV)"],"award-info":[{"award-number":["CIP2019015 (MAVV)"]}]},{"name":"Universidad Central de Chile","award":["CI no. 05\/06"],"award-info":[{"award-number":["CI no. 05\/06"]}]},{"name":"Universidad de Valpara\u00edso","award":["CIP2019015 (MAVV)"],"award-info":[{"award-number":["CIP2019015 (MAVV)"]}]},{"name":"Universidad de Valpara\u00edso","award":["CI no. 05\/06"],"award-info":[{"award-number":["CI no. 05\/06"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The cancer-preventive agent Resveratrol (RSV) [3,5,4\u2032-trihydroxytrans-stilbene] is a widely recognized antioxidant molecule with antitumoral potential against several types of cancers, including prostate, hepatic, breast, skin, colorectal, and pancreatic. Herein, we studied the effect of RSV on the cell viability and invasion potential of gastric cancer cells. AGS and MKN45 cells were treated with different doses of RSV (0\u2013200 \u03bcM) for 24 h. Cell viability was determined using the Sulphorhodamine B dye (SRB) assay. For invasion assays, gastric cells were pre-treated with RSV (5\u201325 \u03bcM) for 24 h and then seeded in a Transwell chamber with coating Matrigel. The results obtained showed that RSV inhibited invasion potential in both cell lines. Moreover, to elucidate the mechanism implicated in this process, we analyzed the effects of RSV on SOD, heparanase, and NF-\u03baB transcriptional activity. The results indicated that RSV increased SOD activity in a dose-dependent manner. Conversely, RSV significantly reduced the DNA-binding activity of NF-\u03baB and the enzymatic activity of heparanase in similar conditions, which was determined using ELISA-like assays. In summary, these results show that RSV increases SOD activity but decreases NF-kB transcriptional activity and heparanase enzymatic activity, which correlates with the attenuation of invasion potential in gastric cancer cells. To our knowledge, no previous study has described the effect of RSV on heparanase activity. This article proposes that heparanase could be a key effector in the invasive events occurring during gastric cancer metastasis.<\/jats:p>","DOI":"10.3390\/molecules27103047","type":"journal-article","created":{"date-parts":[[2022,5,10]],"date-time":"2022-05-10T21:52:11Z","timestamp":1652219531000},"page":"3047","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Resveratrol Decreases the Invasion Potential of Gastric Cancer Cells"],"prefix":"10.3390","volume":"27","author":[{"given":"Daniel","family":"Rojo","sequence":"first","affiliation":[{"name":"Magister en Ciencias M\u00e9dicas Menci\u00f3n Biolog\u00eda Celular y Molecular, Centro de Investigaciones Biom\u00e9dicas, Escuela de Medicina, Universidad de Valpara\u00edso, Angamos 655, Re\u00f1aca, Vi\u00f1a del Mar 2340000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8977-7032","authenticated-orcid":false,"given":"Alejandro","family":"Madrid","sequence":"additional","affiliation":[{"name":"Laboratorio de Productos Naturales y S\u00edntesis Org\u00e1nica (LPNSO), Departamento de Qu\u00edmica, Facultad de Ciencias Naturales y Exactas, Universidad de Playa Ancha, Avda. Leopoldo Carvallo 270, Playa Ancha, Valpara\u00edso 2340000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebasti\u00e1n San","family":"Mart\u00edn","sequence":"additional","affiliation":[{"name":"Magister en Ciencias M\u00e9dicas Menci\u00f3n Biolog\u00eda Celular y Molecular, Centro de Investigaciones Biom\u00e9dicas, Escuela de Medicina, Universidad de Valpara\u00edso, Angamos 655, Re\u00f1aca, Vi\u00f1a del Mar 2340000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3365-3070","authenticated-orcid":false,"given":"Mario","family":"P\u00e1rraga","sequence":"additional","affiliation":[{"name":"Magister en Ciencias M\u00e9dicas Menci\u00f3n Biolog\u00eda Celular y Molecular, Centro de Investigaciones Biom\u00e9dicas, Escuela de Medicina, Universidad de Valpara\u00edso, Angamos 655, Re\u00f1aca, Vi\u00f1a del Mar 2340000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Aparecida","family":"Silva Pinhal","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica, Universidade Federal de S\u00e3o Paulo (UNIFESP), S\u00e3o Paulo 04044-020, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3336-1454","authenticated-orcid":false,"given":"Joan","family":"Villena","sequence":"additional","affiliation":[{"name":"Magister en Ciencias M\u00e9dicas Menci\u00f3n Biolog\u00eda Celular y Molecular, Centro de Investigaciones Biom\u00e9dicas, Escuela de Medicina, Universidad de Valpara\u00edso, Angamos 655, Re\u00f1aca, Vi\u00f1a del Mar 2340000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8833-7382","authenticated-orcid":false,"given":"Manuel","family":"Valenzuela-Valderrama","sequence":"additional","affiliation":[{"name":"Laboratorio de Microbiolog\u00eda Celular, Instituto de Investigaci\u00f3n y Postgrado, Universidad Central de Chile, Lord Cochrane 418, Santiago 8330546, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"394","DOI":"10.3322\/caac.21492","article-title":"Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries","volume":"68","author":"Bray","year":"2018","journal-title":"CA Cancer J. 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