{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,6]],"date-time":"2026-09-06T17:15:32Z","timestamp":1788714932455,"version":"build-2803163510"},"reference-count":76,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2022,5,19]],"date-time":"2022-05-19T00:00:00Z","timestamp":1652918400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key R&amp;D Program of China","award":["2018YFC1706500"],"award-info":[{"award-number":["2018YFC1706500"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>(1) Background: The aerial part of G. uralensis had pharmacological effects against chronic non-bacterial prostatitis (CNP), and flavonoids are the main efficacy components. The purpose of this study was to obtain the pharmacokinetics, prostate distribution and metabolic characteristics of some flavonoids in rats. (2) Methods: The prototype flavones and the metabolites of four representative flavonoids, namely puerarin, luteolin, kaempferol and pinocembrin in plasma, prostate, urine and feces of rats were analyzed by UPLC-Q-Exactive Orbitrap-MS. In addition, the pharmacokinetic parameters in plasma and distribution of prostate of four components were analyzed by HPLC-MS\/MS. (3) Results: In total, 22, 17, 22 and 11 prototype flavones were detected in the prostate, plasma, urine and feces, respectively. The metabolites of puerarin in the prostate are hydrolysis and glucose-conjugated products, the metabolites of kaempferol and luteolin in the prostate are methylation and glucuronidation, and the metabolites of pinocembrin in the prostate are naringenin, oxidation, sulfation, methylation and glucuronidation products. The t1\/2 of puerarin, luteolin, kaempferol and pinocembrin was 6.43 \u00b1 0.20, 31.08 \u00b1 1.17, 18.98 \u00b1 1.46 and 13.18 \u00b1 0.72 h, respectively. The concentrations of the four flavonoids in prostate were ranked as kaempferol &gt; pinocembrin &gt; luteolin &gt; puerarin. (4) Conclusions: Methylation and glucuronidation metabolites were the main metabolites detected in the prostate. A sensitive and validated HPLC\u2013MS\/MS method for simultaneous determination of puerarin, luteolin, kaempferol and pinocembrin in rat plasma and prostate was described, and it was successfully applied to the pharmacokinetic and prostate distribution studies.<\/jats:p>","DOI":"10.3390\/molecules27103245","type":"journal-article","created":{"date-parts":[[2022,5,20]],"date-time":"2022-05-20T00:18:11Z","timestamp":1653005891000},"page":"3245","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Pharmacokinetics, Prostate Distribution and Metabolic Characteristics of Four Representative Flavones after Oral Administration of the Aerial Part of Glycyrrhiza uralensis in Rats"],"prefix":"10.3390","volume":"27","author":[{"given":"Haifan","family":"Liu","sequence":"first","affiliation":[{"name":"Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanhua","family":"Chang","sequence":"additional","affiliation":[{"name":"Beijing Wehand-Bio Pharmaceutical Co., Ltd., Beijing 102629, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenquan","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China"},{"name":"Engineering Research Center of Good Agricultural Practice for Chinese Crude Drugs, Ministry of Education, Beijing 100102, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zuen","family":"Ji","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory for Reserch of Licorice and Products, Korla 841011, China"},{"name":"Xinjiang Quanan Pharmaceutical Co., Ltd., Korla 841011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Cui","sequence":"additional","affiliation":[{"name":"Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifeng","family":"Peng","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory for Reserch of Licorice and Products, Korla 841011, China"},{"name":"Xinjiang Quanan Pharmaceutical Co., Ltd., Korla 841011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Liu, H., Cui, J., Zhang, L., Chang, G., and Wang, W. 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